ID O54593_AMYMD Unreviewed; 3413 AA.
AC O54593;
DT 01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JUN-1998, sequence version 1.
DT 19-JAN-2010, entry version 65.
DE SubName: Full=RifE;
DE SubName: Full=Rifamycin polyketide synthase, type 1;
GN Name=rifE;
OS Amycolatopsis mediterranei (Nocardia mediterranei).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=33910;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98174059; PubMed=9512878; DOI=10.1016/S1074-5521(98)90141-7;
RA August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W.,
RA Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R.,
RA Floss H.G.;
RT "Biosynthesis of the ansamycin antibiotic rifamycin: deductions from
RT the molecular analysis of the rif biosynthetic gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Chem. Biol. 5:69-79(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98165773; PubMed=9497318; DOI=10.1074/jbc.273.11.6030;
RA Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G.;
RT "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the
RT formation of the precursor of mC7N units in rifamycin and related
RT antibiotics.";
RL J. Biol. Chem. 273:6030-6040(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=21201076; PubMed=11278540; DOI=10.1074/jbc.M009667200;
RA Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G.,
RA Leistner E., Floss H.G.;
RT "Mutational analysis and reconstituted expression of the biosynthetic
RT genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the
RT starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei
RT S699.";
RL J. Biol. Chem. 276:12546-12555(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M.,
RA Adman E., Floss H.G.;
RT "Rifamycin insusceptibility: exploring the rif gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R.,
RA Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M.,
RA Hutchinson C.R., Floss H.G.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=LBG A3136;
RA Schupp T., Toupet C., Engel N., Goff S.;
RT "Cloning and sequence analysis of the putative rifamycin polyketide
RT synthase gene cluster from Amycolatopsis mediterranei.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF040570; AAC01714.1; -; Genomic_DNA.
DR EMBL; AJ223012; CAA11039.1; -; Genomic_DNA.
DR PIR; T17467; T17467.
DR HSSP; P25715; 1MLA.
DR SMR; O54593; 30-900, 1592-1684, 2945-3283, 3319-3409.
DR GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR InterPro; IPR001227; Ac_transferase_dom.
DR InterPro; IPR009081; Acyl_carrier_prot-like.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR InterPro; IPR000794; Beta-ketoacyl_synthase.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR006163; Phsphopanteth_bd.
DR InterPro; IPR020842; PKS/FAS_KR.
DR InterPro; IPR020801; PKS_acyl_transferase.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR006162; PPantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR016038; Thiolase-like_subgr.
DR Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 2.
DR Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 2.
DR Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 4.
DR PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF00106; adh_short; 2.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00826; PKS_DH; 2.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 2.
DR PROSITE; PS50075; ACP_DOMAIN; 2.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine; Transferase.
FT CHAIN 1 1701 polyketide synthase module 9.
FT /FTId=PRO_5000053972.
FT CHAIN 31 453 ketoacyl synthase.
FT /FTId=PRO_5000053973.
FT CHAIN 557 875 malonyl acyltransferase.
FT /FTId=PRO_5000053974.
FT CHAIN 887 1087 dehydratase.
FT /FTId=PRO_5000053975.
FT CHAIN 1338 1577 ketoreductase.
FT /FTId=PRO_5000053976.
FT CHAIN 1610 1684 acyl carrier protein.
FT /FTId=PRO_5000053977.
FT CHAIN 1702 3413 polyketide synthase module 10.
FT /FTId=PRO_5000053979.
FT CHAIN 1702 2126 ketoacyl synthase.
FT /FTId=PRO_5000053978.
FT CHAIN 2231 2544 methylmalonyl acyltransferase.
FT /FTId=PRO_5000053980.
FT CHAIN 2554 2754 dehydratase.
FT /FTId=PRO_5000053981.
FT CHAIN 3054 3303 ketoreductase.
FT /FTId=PRO_5000053982.
FT CHAIN 3336 3410 acyl carrier protein.
FT /FTId=PRO_5000053983.
SQ SEQUENCE 3413 AA; 353733 MW; FFA25AC4A3920AA4 CRC64;
MATDEKLLKY LKRVTAELHS LRKQGARHAD EPLAVVGMAC RFPGGVSSPE DLWQLVAGGV
DALSDFPDDR GWELDGLFDP DPDHPGTSYT SQGGFLRGAG LFDAGLFGIS PREALVMDPQ
QRVLLETSWE ALEDAGVDPL SLKGSDVGVF SGVFTQGYGA GAITPDLEAF AGIGAASSVA
SGRVSYVFGL EGPAVTIDTA CSSSLVAIHL AAQALRAGEC SMALAGGATV MPTPGTFVAF
SRQRVLAADG RSKAFSSTAD GTGWAEGAGV LVLERLSVAQ ERGHRILAVL RGSAVNQDGA
SNGLTAPNGP SQQRVIRKAL AGAGLVASDV DVVEAHGTGT ALGDPIEAQA LLATYGQGRE
RPLWLGSVKS NFGHTQAAAG VAGVIKMVQA LRHGAMPPTL HVAEPTPEVD WSAGAVELLT
EPREWPAGDR PRRAGVSAFG ISGTNAHLIL EEAPPADAVA EEPEFKGPVP LVVSAGSPTS
LAAQAGRLAE VLASGGVSRA RLASGLLSGR ALLGDRAVVV AGTDEDAVAG LRALARGDRA
PGVLTGSAKH GKVVYVFPGQ GSQRLGMGRE LYDRYPVFAT AFDEACEQLD VCLAGRAGHR
VRDVVLGEVP AETGLLNQTV FTQAGLFAVE SALFRLAESW GVRPDVVLGH SIGEITAAYA
AGVFSLPDAA RIVAARGRLM QALAPGGAMV AVAASEAEVA ELLGDGVELA AVNGPSAVVL
SGDADAVVAA AARMRERGHK TKQLKVSHAF HSARMAPMLA EFAAELAGVT WREPEIPVVS
NVTGRFAEPG ELTEPGYWAE HVRRPVRFAE GVAAATESGG SLFVELGPGA ALTALVEETA
EVTCVAALRD DRPEVTALIT AVAELFVRGV AVDWPALLPP VTGFVDLPKY AFDQQHYWLQ
PAAQATDAAS LGQVAADHPL LGAVVRLPQS DGLVFTSRLS LKSHPWLADH VIGGVVLVAG
TGLVELAVRA GDEAGCPVLE ELVIEAPLVV PDHGGVRIQV VVGAPGETGS RAVEVYSLRE
DAGAEVWARH ATGFLAATPS QHKPFDFTAW PPPGVERVDV EDFYDGLVDR GYAYGPSFRG
LRAVWRRGDE VFAEVALAED DRADAARFGI HPGLLDAALH AGMAGATTTE EPGRPVLPFA
WNGLVLHAAG ASALRVRLAP SGPDALSVEA ADEAGGLVVT ADSLVSRPVS AEQLGAAANH
DALFRVEWTE ISSAGDVPAD HVEVLEAVGE DPLELTGRVL EAVQTWLADA ADDARLVVVT
RGAVHEVTDP AGAAVWGLIR AAQAENPDRI VLLDTDGEVP LGRVLATGEP QTAVRGATLF
APRLARAEAA EAPAVTGGTV LISGAGSLGA LTARHLVARH GVRRLVLVSR RGPDADGMAE
LTAELIAQGA EVAVVACDLA DRDQVRVLLA EHRPNAVVHT AGVLDDGVFE SLTRERLAKV
FAPKVTAANH LDELTRELDL RAFVVFSSAS GVFGSAGQGN YAAANAYLDA VVANRRAAGL
PGTSLAWGLW EQTDGMTAHL GDADQARASR GGVLAISPAE GMELFDAAPD GLVVPVKLDL
RKTRAGGTVP HLLRGLVRPG RQQARPASTV DNGLAGRLAG LAPAEQEALL LDVVRTQVAL
VLGHAGPEAV RADTAFKDTG FDSLTSVELR NRLREASGLK LPATLVFDYP TPVALARYLR
DELGDTVATT PVATAAAADA GEPIAIVGMA CRLPGGVTDP EGLWRLVRDG LEGLSPFPED
RGWDLENLFD DDPDRSGTTY TSRGGFLDGA GLFDAGFFGI SPREALAMDP QQRLLLEAAW
EALEGTGVDP GSLKGADVGV FAGVSNQGYG MGADPAELAG YASTAGASSV VSGRVSYVFG
FEGPAVTIDT ACSSSLVAMH LAGQALRQGE CSMALAGGVT VMGTPGTFVE FAKQRGLAGD
GRCKAYAEGA DGTGWAEGVG VVVLERLSVA RERGHRVLAV LRGSAVNSDG ASNGLTAPNG
PSQQRVIRRA LAGAGLEPSD VDIVEGHGTG TALGDPIEAQ ALLATYGKDR DPETPLWLGS
VKSNFGHTQS AAGVAGVIKM VQALRHGVMP PTLHVDRPTS QVDWSAGAVE VLTEAREWPR
NGRPRRAGVS SFGISGTNAH LIIEEAPAEP QLAGPPPDGG VVPLVVSARS PGALAGQARR
LATFLGDGPL SDVAGALTSR ALFGERAVVV ADSAEEARAG LGALARGEDA PGLVRGRVPA
SGLPGKLVWV FPGQGTQWVG MGRELLEESP VFAERIAECA AALEPWIGWS LFDVLRGDGD
LDRVDVLQPA CFAVMVGLAA VWSSAGVVPD AVLGHSQGEI AAACVSGALS LEDAAKVVAL
RSQAIAAKLS GRGGMASVAL GEADVVSRLA DGVEVAAVNG PASVVIAGDA QALDETLEAL
SGAGIRARRV AVDYASHTRH VEDIEDTLAE ALAGIDARAP LVPFLSTLTG EWIRDEGVVD
GGYWYRNLRG RVRFGPAVEA LLAQGHGVFV ELSAHPVLVQ PITELTDETA AVVTGSLRRD
DGGLRRLLTS MAELFVRGVE VDWTSLVPPA RADLPTYAFD HEHYWLRAAD TASDAVSLGL
AGADHPLLGA VVQLPQSDGL VFTSRLSLRS HPWLADHAVR DVVIVPGTGL VELAVRAGDE
AGCPVLDELV IEAPLVVPRR GGVRVQVALG GPADDGSRTV DVFSLREDAD SWLRHATGVL
VPENRPRGTA AFDFAAWPPP EAKPVDLTGA YDVLADVGYG YGPTFRAVRA VWRRGSGNTT
ETFAEIALPE DARAEAGRFG IHPALLDAAL HSTMVSAAAD TESYGDEVRL PFAWNGLRLH
AAGASVLRVR VAKPERDSLS LEAVDESGGL VVTLDSLVGR PVSNDQLTTA AGPAGAGSLY
RVDWTPLSSV DTSGRVPSWL PVATAEEVAT LADDVLTGAT EAPAVAVMEA VADEGSVLAL
TVRVLDVVQC WLAGGGLEGT KLAIVTRGAV PAGDGVVHDP AAAAVWGLVR AAQAENPDRI
VLLDVEPEAD VPPLLGSVLA DGEPQVAVRG TTLSIPRLAR AARPDPAAGF KTRGPVLVTG
GTGSLGGLVA RHLVERHGVR QLVLASRRGL DAEGAKDLVT DLTALGADVA VAACDVADRD
QVAALLTEHR PSAVVHTAGV PDAGVIGTVT PDRLAEVFAP KVTAARHLDE LTRDLDLDSF
VVYSSVSAVF MGAGSGSYAA ANAYLDGLMA HRRAAGLPGQ SLAWGLWDQT TGGMAAGTDE
AGRARMTRRG GLVAMKPAAG LDLFDAAIGS GEPLLVPAQL DLRGLRAEAA GGTEVPHLLR
GLVRAGRQQA RAASTVEENW AGRLAGLEPA ERGQVLLELV RAQVAGVLGY RAAHQVDPDQ
GLFEIGFDSL TAIELRNRLR ARTERKISPG VVFDHPTPAL LAAHLNELLR KKV
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