ID O54666_AMYMD Unreviewed; 4735 AA.
AC O54666;
DT 01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JUN-1998, sequence version 1.
DT 19-JAN-2010, entry version 62.
DE SubName: Full=RifA;
DE SubName: Full=Rifamycin polyketide synthase, type 1;
GN Name=rifA;
OS Amycolatopsis mediterranei (Nocardia mediterranei).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=33910;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98174059; PubMed=9512878; DOI=10.1016/S1074-5521(98)90141-7;
RA August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W.,
RA Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R.,
RA Floss H.G.;
RT "Biosynthesis of the ansamycin antibiotic rifamycin: deductions from
RT the molecular analysis of the rif biosynthetic gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Chem. Biol. 5:69-79(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=98165773; PubMed=9497318; DOI=10.1074/jbc.273.11.6030;
RA Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G.;
RT "3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the
RT formation of the precursor of mC7N units in rifamycin and related
RT antibiotics.";
RL J. Biol. Chem. 273:6030-6040(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RX MEDLINE=21201076; PubMed=11278540; DOI=10.1074/jbc.M009667200;
RA Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G.,
RA Leistner E., Floss H.G.;
RT "Mutational analysis and reconstituted expression of the biosynthetic
RT genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the
RT starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei
RT S699.";
RL J. Biol. Chem. 276:12546-12555(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M.,
RA Adman E., Floss H.G.;
RT "Rifamycin insusceptibility: exploring the rif gene cluster of
RT Amycolatopsis mediterranei S699.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S699;
RA Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R.,
RA Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M.,
RA Hutchinson C.R., Floss H.G.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=LBG A3136;
RA Schupp T., Toupet C., Engel N., Goff S.;
RT "Cloning and sequence analysis of the putative rifamycin polyketide
RT synthase gene cluster from Amycolatopsis mediterranei.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AF040570; AAC01710.1; -; Genomic_DNA.
DR EMBL; AJ223012; CAA11035.1; -; Genomic_DNA.
DR PIR; T17463; T17463.
DR HSSP; P08659; 1LCI.
DR GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR InterPro; IPR001227; Ac_transferase_dom.
DR InterPro; IPR009081; Acyl_carrier_prot-like.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR000794; Beta-ketoacyl_synthase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR006163; Phsphopanteth_bd.
DR InterPro; IPR020842; PKS/FAS_KR.
DR InterPro; IPR020801; PKS_acyl_transferase.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR006162; PPantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR016038; Thiolase-like_subgr.
DR Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 3.
DR Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 2.
DR Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 6.
DR PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 4.
DR PROSITE; PS50075; ACP_DOMAIN; 4.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine; Transferase.
FT CHAIN 1 631 polyketide synthase loading domain.
FT /FTId=PRO_5000053932.
FT CHAIN 632 2194 3-AHBA CoA ligase module 1.
FT /FTId=PRO_5000053934.
FT CHAIN 632 1054 ketoacyl synthase.
FT /FTId=PRO_5000053933.
FT CHAIN 1147 1456 methylmalonyl acyltransferase.
FT /FTId=PRO_5000053935.
FT CHAIN 1467 1622 dehydratase.
FT /FTId=PRO_5000053936.
FT CHAIN 1829 2075 ketoreductase.
FT /FTId=PRO_5000053937.
FT CHAIN 2104 2178 acyl carrier protein.
FT /FTId=PRO_5000053938.
FT CHAIN 2195 3171 polyketide synthase module 2.
FT /FTId=PRO_5000053940.
FT CHAIN 2195 2618 ketoacyl synthase.
FT /FTId=PRO_5000053939.
FT CHAIN 2718 3022 malonyl acyltransferase.
FT /FTId=PRO_5000053941.
FT CHAIN 3081 3155 acyl carrier protein.
FT /FTId=PRO_5000053942.
FT CHAIN 3172 4735 polyketide synthase module 3.
FT /FTId=PRO_5000053944.
FT CHAIN 3172 3596 ketoacyl synthase.
FT /FTId=PRO_5000053943.
FT CHAIN 3697 4011 methylmalonyl acyltransferase.
FT /FTId=PRO_5000053945.
FT CHAIN 4307 4548 ketoreductase.
FT /FTId=PRO_5000053946.
FT CHAIN 4580 4654 acyl carrier protein.
FT /FTId=PRO_5000053947.
SQ SEQUENCE 4735 AA; 495219 MW; 0B717178FB68C39C CRC64;
MRTDLIKPLH VALLENATRF AGKPAFADDH RTVTYGDLEA RTRRLAGHLA GLGVRHGDRV
AICLGNRVST VESYFAILRA GAVGVPLNPG SATAELEHPL TDSGATVVVT DAAQAARLRL
APHVELLVTG DDVPEGAHSY DELALSEPAE PAADDLELDE PAWMFYTSGT TGRPKGVVST
QRNCLWSVAS CYVPFPGLSD QDRVLWPLPL FHSLSHIACV LSATVVGASV RIADGSSADD
VMRLIEAESS TFLAGVPTTY HHLVRAARQR GFSAPSLRIG LAGGAVLGAG LRSEFEETFG
VPLIDAYGST ETCGAITMNP PDGARVEGSC GLAVPGVDVR VVDPDTGLDV PAGEEGEVWV
SGPNVMLGYH NSPEATAAAM RDGWFRTGDL ARRDDAGYFT ICGRIKELII RGGANIHPGE
VEAVLRTVDG VADAAVGGVP HDTLGEVPVA YVIPGPTGFD PAALIEKCRE QLSAYKVPDR
ILEVAHIPRT ASGKIRRGLL TDEPAQLRYA ATEHEEQSRH ADESVAAALR ARLSGLDERA
QCELLEDLVR TQAADVLGQP VPDGRAFRDL GFTSLAIVEL RNRLTEHTGL WLPASAVFDH
PTPAALAARV RAELLGITQA VAEPVVAADP GEPIAIVGMA CRLPGGVASP EDLWRLVAER
VDAVSEFPGD RGWDLDSLID PDRERAGTSY VGQGGFLHDA GEFDAGFFGI SPREAVAMDP
QQRLLLETSW EALENAGVDP IALKGTDTGV FSGLMGQGYG SGAVAPELEG FVTTGVASSV
ASGRVSYVLG LEGPAVTVDT ACSSSLVAMH LAAQALRQGE CSMALAGGVT VMATPGSFVE
FSRQRALAPD GRCKAFAAAA DGTGWSEGVG VVVLERLSVA RERGHRILAV LRGSAVNQDG
ASNGLTAPNG LSQQRVIRRA LAAAGLAPSD VDVVEAHGTG TTLGDPIEAQ ALLATYGQER
KQPLWLGSLK SNIGHAQAAA GVAGVIKMVQ ALRHETLPPT LHVDKPTLEV DWSAGAIELL
TEARAWPRNG RPRRAGVSSF GVSGTNAHLI LEEAPAEEPV AAPELPVVPL VVSARSTESL
SGQAERLASL LEGDVSLTEV AGALVSRRAV LDERAVVVAG SREEAVTGLR ALNTAGSGTP
GKVVWVFPGQ GTQWAGMGRE LLAESPVFAE RIAECAAALA PWIDWSLVDV LRGEGDLGRV
DVLQPACFAV MVGLAAVWES VGVRPDAVVG HSQGEIAAAC VSGALSLEDA AKVVALRSQA
IAAELSGRGG MASVALGEDD VVSRLVDGVE VAAVNGPSSV VIAGDAHALD ATLEILSGEG
IRVRRVAVDY ASHTRHVEDI RDTLAETLAG ISAQAPAVPF YSTVTSEWVR DAGVLDGGYW
YRNLRNQVRF GAAATALLEQ GHTVFVEVSA HPVTVQPLSE LTGDAIGTLR REDGGLRRLL
ASMGELFVRG IDVDWTAMVP AAGWVDLPTY AFEHRHYWLE PAEPASAGDP LLGTVVSTPG
SDRLTAVAQW SRRAQPWAVD GLVPNAALVE AAIRLGDLAG TPVVGELVVD APVVLPRRGS
REVQLIVGEP GEQRRRPIEV FSREADEPWT RHAHGTLAPA AAAVPEPAAA GDATDVTVAG
LRDADRYGIH PALLDAAVRT VVGDDLLPSV WTGVSLLASG ATAVTVTPTA TGLRLTDPAG
QPVLTVESVR GTPFVAEQGT TDALFRVDWP EIPLPTAETA DFLPYEATSA EATLSALQAW
LADPAETRLA VVTGDCTEPG AAAIWGLVRS AQSEHPGRIV LADLDDPAVL PAVVASGEPQ
VRVRNGVASV PRLTRVTPRQ DARPLDPEGT VLITGGTGTL GALTARHLVT AHGVRHLVLV
SRRGEAPELQ EELTALGASV AIAACDVADR AQLEAVLRAI PAEHPLTAVI HTAGVLDDGV
VTELTPDRLA TVRRPKVDAA RLLDELTREA DLAAFVLFSS AAGVLGNPGQ AGYAAANAEL
DALARQRNSL DLPAVSIAWG YWATVSGMTE HLGDADLRRN QRIGMSGLPA DEGMALLDAA
IATGGTLVAA KFDVAALRAT AKAGGPVPPL LRGLAPLPRR AAAKTASLTE RLAGLAETEQ
AAALLDLVRR HAAEVLGHSG AESVHSGRTF KDAGFDSLTA VELRNRLAAA TGLTLSPAMI
FDYPKPPALA DHLRAKLFGS AANRPAEIGT AAAEEPIAIV AMACRFPGGV HSPEDLWRLV
ADGADAVTEF PADRGWDTDR LYHEDPDHEG TTYVRHGAFL DDAAGFDAAF FGISPNEALA
MDPQQRLLLE TSWELFERAA IDPTTLAGQD IGVFAGVNSH DYSMRMHRAA GVEGFRLTGG
SASVLSGRVA YHFGVEGPAV TVDTACSSSL VALHMAVQAL QRGECSMALA GGVMVMGTVE
TFVEFSRQRG LAPDGRCKAF ADGADGTGWS EGVGLLLVER LSEAQRRGHQ VLAVVRGSAV
NSDGASNGLT APNGPSQQRV IRKALAAAGL STSDVDAVEA HGTGTTLGDP IEAEALLATY
GQNRETPLWL GSVKSNLGHT QAAAGVAGVI KMVMAMRHGV LPRTLHVDRP SSYVDWSAGA
VELLTEARDW VSNGHPRRAG VSSFGIGGTN AHVVLEEVAA PITTPQPEPA EFLVPVLVSA
RTAAGLRGQA GRLAAFLGDR TDVRVPDAAY ALATTRAQLD HRAVVLASDR AQLCADLAAF
GSGVVTGTPV DGKLAVLFTG QGSQWAGMGR ELAETFPVFR DAFEAACEAV DTHLRERPLR
EVVFDDSALL DQTMYTQGAL FAVETALFRL FESWGVRPGL LAGHSIGELA AAHVSGVLDL
ADAGELVAAR GRLMQALPAG GAMVAVQATE DEVAPLLDGT VCVAAVNGPD SVVLSGTEAA
VLAVADELAG RGRKTRRLAV SHAFHSPLME PMLDDFRAVA ERLTYRAGSL PVVSTLTGEL
AALDSPDYWV GQVRNAVRFS DAVTALGAQG ASTFLELGPG GALAAMALGT LGGPEQSCVA
TLRKNGAEVP DVLTALAELH VRGVGVDWTT VLDEPATAVG TVLPTYAFQH QRFWVDVDET
AAVSVTPPPA EPIVDRPVQD VLELVRESAA VVLGHRDAGS FDLDRSFKDH GFDSLSAVKL
RNRLRDFTGV ELPSTLIFDY PNPAVLADHL RAELLGERPA APAPVTRDVS DEPIAIVGMS
TRLPGGADSP EELWKLVAEG RDAVSGFPVD RGWDLDGLYH PDPAHAGTSY TRSGGFLHDA
AQFDAGLFGI SPREALAMDP QQRLLLETSW EALERAGVDP LSARGSDVGV FTGIVHHDYV
TRLREVPEDV QGYTMTGTAS SVASGRVAYV FGFEGPAVTV DTACSSSLVA MHLAAQALRQ
GECSMALAGG ATVMASPDAF LEFSRQRGLS ADGRCKAYAE GADGTGWAEG VGVVVLERLS
VARERGHRVL AVLRGSAVNQ DGASNGLTAP NGPSQQRVIR GALASAGLAP SDVDVVEGHG
TGTALGDPIE VQALLATYGQ EREQPLWLGS LKSNLGHTQA AAGVVGVIKM IMAMRHGVMP
ATLHVDERTS QVDWSAGAIE VLTEAREWPR TGRPRRAGVS SFGASGTNAH LIIEEGPAEE
AVDEEVASVV PLVVSARSAG SLAGQAGRLA AVLENESLAG VAGALVSGRA TLNERAVVIA
GSRDEAQDGL QALARGENAP GVVTGTAGKP GKVVWVFPGQ GSQWMGMGRD LLDSSPVFAA
RIKECAAALE QWTDWSLLDV LRGDADLLDR VDVVQPASFA MMVGLAAVWT SLGVTPDAVL
GHSQGEIAAA CVSGALSLDD AAKVVALRSQ AIAGELAGRG GMASVALSEE DAVARLTPWA
NRVEVAAVNS PSSVVIAGDA QALDEALEAL AGDGVRVRRV AVDYASHTRH VEAIAETLAK
TLAGIDARVP AIPFYSTVLG TWIEQAVVDA GYWYRNLRQQ VRFGPSVADL AGLGHTVFVE
ISAHPVLVQP LSEISDDAVV TGSLRRDDGG LRRLLASAAE LYVRGVAVDW TAAVPAAGWV
DLPTYAFDRR HFWLHEAETA EAAEGMDGEF WTAIEQSDVD SLAELLELVP EQRGALSTVV
PVLAQWRDRR RERSTAEKLR YQVTWQPLER EAAGVPGGRW LAVVPAGTTD ALLKELTGQG
LDIVRLEIEE ASRAQLAEQL RNVLAEHDLT GVLSLLALDG GPADAAEITA STLALVQALG
DTTTSAPLWC LTSGAVNIGI QDAVTAPAQA AVWGLGRAVA LERLDRWGGL VDLPAAIDAR
TAQALLGVLN GAAGEDQLAV RRSGVYRRRL VRKPVPESAT SRWEPRGTVL VTGGAEGLGR
HASVWLAQSG AERLIVTGTD GVDELTAELA EFGTTVEFCA DTDRDAIAQL VADSEVTAVV
HAADIAQTSS VDDTGVADLD EVFAAKVTTA VWLDQLFEDT PLDAFVVFSS IAGIWGGGGQ
GPAGAANAVL DALVEWRRAR GLKATSIAWG ALDQIGIGMD EAALAQLRRR GVIPMAPPLA
VTAMVQAVAG NEKAVAVADM DWAAFIPAFT SVRPSPLFAD LPEAKAILRA AQDDGEDGDT
ASSLADSLRA VPDAEQNRIL LKLVRGHAST VLGHSGAEGI GPRQAFQEVG FDSLAAVNLR
NSLHAATGLR LPATLIFDYP TPEALVGYLR VELLREADDG LDGREDDLRR VLAAVPFARF
KEAGVLDTLL GLADTGTEPG TDAETTEAAP AADDAELIDA LDISGLVQRA LGQTS
//
