ID Q53670_STRAA Unreviewed; 150 AA.
AC Q53670;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 19-JAN-2010, entry version 27.
DE SubName: Full=Cyclase;
GN Name=mtmX;
OS Streptomyces argillaceus.
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Streptomycineae; Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=41951;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=96257259; PubMed=8654997; DOI=10.1016/0378-1119(96)00029-7;
RA Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A.;
RT "Characterization of Streptomyces argillaceus genes encoding a
RT polyketide synthase involved in the biosynthesis of the antitumor
RT mithramycin.";
RL Gene 172:87-91(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=96335144; PubMed=8757400; DOI=10.1007/s004380050218;
RA Fernandez E., Lombo F., Mendez C., Salas J.A.;
RT "An ABC transporter is essential for resistance to the antitumor agent
RT mithramycin in the producer Streptomyces argillaceus.";
RL Mol. Gen. Genet. 251:692-698(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=97294479; PubMed=9150235;
RA Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A.;
RT "Cloning and insertional inactivation of Streptomyces argillaceus
RT genes involved in the earliest steps of biosynthesis of the sugar
RT moieties of the antitumor polyketide mithramycin.";
RL J. Bacteriol. 179:3354-3357(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=98406047; PubMed=9733697;
RA Fernandez E., Weissbach U., Sanchez Reillo C., Brana A.F., Mendez C.,
RA Rohr J., Salas J.A.;
RT "Identification of two genes from Streptomyces argillaceus encoding
RT glycosyltransferases involved in transfer of a disaccharide during
RT biosynthesis of the antitumor drug mithramycin.";
RL J. Bacteriol. 180:4929-4937(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=99200478; PubMed=10102355; DOI=10.1007/s004380050960;
RA Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A.;
RT "Analysis of two chromosomal regions adjacent to genes for a type II
RT polyketide synthase involved in the biosynthesis of the antitumor
RT polyketide mithramycin in Streptomyces argillaceus.";
RL Mol. Gen. Genet. 261:216-225(1999).
RN [6]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=99111236; PubMed=9889148; DOI=10.1016/S1074-5521(99)80017-9;
RA Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M.,
RA Brana A.F., Mendez C., Rohr J., Salas J.A.;
RT "Oxidative cleavage of premithramycin B is one of the last steps in
RT the biosynthesis of the antitumor drug mithramycin.";
RL Chem. Biol. 6:19-30(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=20123456; PubMed=10660060; DOI=10.1007/PL00008667;
RA Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U.,
RA Kunzel E., Rohr J., Mendez C., Salas J.A.;
RT "Characterization of two glycosyltransferases involved in early
RT glycosylation steps during biosynthesis of the antitumor polyketide
RT mithramycin by Streptomyces argillaceus.";
RL Mol. Gen. Genet. 262:991-1000(2000).
RN [8]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=21148776; PubMed=11254130; DOI=10.1007/s004380000372;
RA Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L.,
RA Brana A.F., Kunzel E., Rohr J., Mendez C., Salas J.A.;
RT "The mtmVUC genes of the mithramycin gene cluster in Streptomyces
RT argillaceus are involved in the biosynthesis of the sugar moieties.";
RL Mol. Gen. Genet. 264:827-835(2001).
RN [9]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=22619809; PubMed=12733914; DOI=10.1021/ja034162h;
RA Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J.,
RA Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J.;
RT "Mithramycin SK, a novel antitumor drug with improved therapeutic
RT index, mithramycin SA, and demycarosyl-mithramycin SK: three new
RT products generated in the mithramycin producer Streptomyces
RT argillaceus through combinatorial biosynthesis.";
RL J. Am. Chem. Soc. 125:5745-5753(2003).
RN [10]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=99115752; PubMed=9917296; DOI=10.1021/np980355k;
RA Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J.;
RT "The structure of mithramycin reinvestigated.";
RL J. Nat. Prod. 62:119-121(1999).
RN [11]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=21843297; PubMed=11853433; DOI=10.1021/ja0105156;
RA Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U.,
RA Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J.;
RT "Ketopremithramycins and ketomithramycins, four new aureolic acid-type
RT compounds obtained upon inactivation of two genes involved in the
RT biosynthesis of the deoxysugar moieties of the antitumor drug
RT mithramycin by Streptomyces argillaceus, reveal novel insights into
RT post-PKS tailoring steps of the mithramycin biosynthetic pathway.";
RL J. Am. Chem. Soc. 124:1606-1614(2002).
RN [12]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=20119254; PubMed=10652287; DOI=10.1074/jbc.275.5.3065;
RA Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E.,
RA Sanchez C., Mendez C., Rohr J., Salas J.A.;
RT "Characterization of two polyketide methyltransferases involved in the
RT biosynthesis of the antitumor drug mithramycin by Streptomyces
RT argillaceus.";
RL J. Biol. Chem. 275:3065-3074(2000).
RN [13]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=20243461; PubMed=10779713;
RX DOI=10.1111/j.1574-6968.2000.tb09082.x;
RA Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A.;
RT "Insertional inactivation of mtrX and mtrY genes from the mithramycin
RT gene cluster affects production and growth of the producer organism
RT Streptomyces argillaceus.";
RL FEMS Microbiol. Lett. 186:61-65(2000).
RN [14]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=22017994; PubMed=12022840; DOI=10.1021/ja017385l;
RA Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F.,
RA Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A.;
RT "Rationally designed glycosylated premithramycins: hybrid aromatic
RT polyketides using genes from three different biosynthetic pathways.";
RL J. Am. Chem. Soc. 124:6056-6062(2002).
RN [15]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=22697398; PubMed=12813091;
RX DOI=10.1128/JB.185.13.3962-3965.2003;
RA Rodriguez D., Quiros L.M., Brana A.F., Salas J.A.;
RT "Purification and characterization of a monooxygenase involved in the
RT biosynthetic pathway of the antitumor drug mithramycin.";
RL J. Bacteriol. 185:3962-3965(2003).
RN [16]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=97113238; PubMed=8807845; DOI=10.1016/S1074-5521(96)90262-8;
RA Blanco G., Fu H., Mendez C., Khosla C., Salas J.A.;
RT "Deciphering the biosynthetic origin of the aglycone of the aureolic
RT acid group of anti-tumor agents.";
RL Chem. Biol. 3:193-196(1996).
RN [17]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX PubMed=14660589; DOI=10.1074/jbc.M312351200;
RA Rodriguez D., Quiros L.M., Salas J.A.;
RT "MtmMII-mediated C-methylation during biosynthesis of the antitumor
RT drug mithramycin is essential for biological activity and DNA-drug
RT interaction.";
RL J. Biol. Chem. 279:8149-8158(2004).
RN [18]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX MEDLINE=98042823; PubMed=9375253; DOI=10.1016/S1074-5521(97)90313-6;
RA Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C.,
RA Ylihonko K., Mantsala P., Salas J.;
RT "Folding of the polyketide chain is not dictated by minimal polyketide
RT synthase in the biosynthesis of mithramycin and anthracycline.";
RL Chem. Biol. 4:751-755(1997).
RN [19]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX PubMed=15712316; DOI=10.1002/cbic.200400309;
RA Nur-e-Alam M., Mendez C., Salas J.A., Rohr J.;
RT "Elucidation of the glycosylation sequence of mithramycin
RT biosynthesis: isolation of 3A-deolivosylpremithramycin B and its
RT conversion to premithramycin B by glycosyltransferase MtmGII.";
RL Chembiochem 6:632-636(2005).
RN [20]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RX PubMed=10760873;
RX DOI=10.1002/(SICI)1521-3773(20000218)39:4<796::AID-ANIE796>3.0.CO;2-N;
RA Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J.,
RA Bearden D., Mendez C., Salas J.A., Rohr J.;
RT "The Novel Hybrid Antitumor Compound Premithramycinone H Provides
RT Indirect Evidence for a Tricyclic Intermediate of the Biosynthesis of
RT the Aureolic Acid Antibiotic Mithramycin.";
RL Angew. Chem. Int. Ed. 39:796-799(2000).
RN [21]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956;
RA Lombo F.;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; X89899; CAA61988.1; -; Genomic_DNA.
DR PIR; S58172; S58172.
DR SMR; Q53670; 17-148.
DR GO; GO:0005622; C:intracellular; IEA:InterPro.
DR GO; GO:0006810; P:transport; IEA:InterPro.
DR InterPro; IPR002075; NTF2.
DR Pfam; PF02136; NTF2; 1.
PE 4: Predicted;
SQ SEQUENCE 150 AA; 16494 MW; 83766BA1597FE00D CRC64;
MTAPTETQPH SSAGFGSLYA EIQQFYARHM QLLDGGAADE WAADFTEDAS FSVPTVPAPV
VGRDALAAAV RRTAESLAST GEQHRHWPGV FDVRPRRDGS VVVHSYTTVY ASPRGGESRV
HRVCTCTDVL VREDGRLLVR SREVIRDDLR
//
