ID   Q8KUH3_ACTPA            Unreviewed;      4684 AA.
AC   Q8KUH3;
DT   01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2002, sequence version 1.
DT   19-JAN-2010, entry version 41.
DE   SubName: Full=Polyketide synthase;
GN   Name=asmA;
OS   Actinosynnema pretiosum subsp. auranticum.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Actinosynnemataceae; Actinosynnema.
OX   NCBI_TaxID=42198;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 31565;
RX   MEDLINE=22056096; PubMed=12060743; DOI=10.1073/pnas.092697199;
RA   Yu T.W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J.,
RA   Moss S.J., Leistner E., Floss H.G.;
RT   "The biosynthetic gene cluster of the maytansinoid antitumor agent
RT   ansamitocin from Actinosynnema pretiosum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:7968-7973(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 31565;
RA   Yu T.-W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q.,
RA   Xu J., Moss S.J., Leistner E., Floss H.G.;
RL   Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AF453501; AAM54075.1; -; Genomic_DNA.
DR   HSSP; P96202; 1PQW.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR011032; GroES-like.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 2.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 3.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 4.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 2.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00106; adh_short; 2.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF00109; ketoacyl-synt; 2.
DR   Pfam; PF02801; Ketoacyl-synt_C; 2.
DR   Pfam; PF00550; PP-binding; 3.
DR   SMART; SM00827; PKS_AT; 2.
DR   SMART; SM00826; PKS_DH; 2.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00822; PKS_KR; 2.
DR   SMART; SM00825; PKS_KS; 2.
DR   SMART; SM00823; PKS_PP; 3.
DR   PROSITE; PS50075; ACP_DOMAIN; 3.
DR   PROSITE; PS00455; AMP_BINDING; 1.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   4684 AA;  483399 MW;  C36307EEAFEFE059 CRC64;
     MLRSDLIRPL PELLREHAAE RGDRVAFRDA RRAVTWVDLE LRTRRLAGHL AARRLLPGDR
     AVILLGNRVE TVECYLALVR AGAVGVPLNP VSTDAELGAL LDDSGARLVL TDAAHADQLA
     GLRATRPALE VVHVDRDYEA LAGVDAPLPA HDALELDDLA WMLYTSGTTG APKGVLSTQR
     NCLWSVAACY APVLGLTPDD RVLWPLPLFH SLSHIAGVLA TTAVGATTRV VDGRSPQDVL
     AALHEERSTV LAGVPTTYHH LVRAAGERGF LAPDLRVGLV GGAITTAELR AAVEQRFGVP
     LIDAYGSTET CGSITVNWPT GPRVEGSSGL PVPGLAVRLV DPDTGLDAPT GAEGEVWVRG
     PSVMVGYHNR PEATAAALRD GWYRTGDLAT RDESGFHAVT GRIDDVVVRG GEKVHPAEVE
     AVLRAVPGVA DAAVVGRPHD VLGEVPVAFL VPGEGFDPAQ VLAVCRERLS YHKVPEELYQ
     IESVPRTASG KITRRVLLDT PARLRASGER GALFRTDWVP LAAAPGGATA PGAVAGSGAA
     TASSAVTGAG AATGPARATT PAAATAPATT PAEASTPVAA ARPGGHPVAV MAEPTAPAPG
     GAPVVLDLRG DDAPALSGRP DLVLTRGAVA VDGGPVRLGQ LAAWTAACRA GITAVDADDD
     GAVDPVGLLA AGEPRLLVRS GVVLVPRLHE VPGSVVGRGA LDPRRAVAVT GADSGTAAAL
     ARHLRGAHGV RDLLLITAPH GRARAGALRD DLVASGARVV LAVAGPDALD RALSRAPSPL
     GALVHTGDGD PEALPRLLGA RVELVVCATA FDSAAAEDVV LRRRAAGMPA RCLAWGPWEA
     ETAQARPGLA VLPLRDGLAA FDAALGADEP VVSVLRPDPD LVAGDVPAPL RDLLHVVAAE
     PEPVRVAELR ASLAGRGERE RRRRLLDLVR AETAALRPAE FLVDGHERGF AELGFTSATS
     VALRNRLTAA TGLALPATAA FEHPTPTALA ALLHDELFGA SRTPVAPARA VERPVERSGE
     PIAVVAAGCR LPGGVASPED LWEVVRSGAD VVSPFPVDRG WDLDALRGAC AGGGFLADAA
     LFDAALFGVH PREALAMDPQ QRLLLEVAWE VFERAGIDPR SVAGDGTGVF AGLMGHDYGS
     RPGLDRQLVE GYLSTGNAGS VASGRIAYAF GLTGPALTVD TACSSSLVAV HLAVQSLRRG
     ECSMALAGGA TVMATPADFV EFARQGALAP DGRCKPFSAS ADGTAWSEGA GLVLLERLSD
     ARRNGRRVLA VIRGSAVNSD GASNGLTAPS GAAQQRVIRA ALADAGLSTS DVDAVEAHGT
     GTRLGDPIEA RALVATYGRD RDGEPLWLGS LKSNFGHPQG AAGIAGLIKL VQALRHGVLP
     RTLHVDAPTP EVDWDGVEVL AEQRAWPEVD RPRRAAVSSF GVSGTNAHVV LEQAPAETAA
     PAAPERGPVP LPVSAVDAAA LRAQARRLAD FLAPSDVTAA ELAGALRAGR ADLGERAVVV
     AADRAGALAA LEALAADEPS PALVTGRADV TGRTVLVFPG QGGQWVGMGA RLLDESPVFA
     ERVGECAAAV DPLVDFRVVD VLRGGDLSRV DVVQPVAFVV MVALARLWRS VGVEPDAVVG
     HSQGEIAAAC VAGALSLADA ARVVVLRSRA IAAGLNGGGM ASIPLPQAQV EELVAGTGVC
     VAAVNGPGSV VVSGEVDALV ARVEGARRLP VDYASHSPAV DVLREGLLAD LAPITPRPAA
     IPMLSTVTGK WLDGAELDAD YWFANLRHPV RFADATALLV ADGFRAFVEV GAHPVLTAPV
     QDLLAGAGPS VATGTLRRDD GGLDRFLRSA AELHVRGVPV DLSPLAPPPV RALDLPTYPF
     QRARYWLDAD PTAASALRHP LLTSATDLAG GEDLVCQARL STARHPWLAD HAVRGSAVLP
     GTCWAELAIR AGDEVGAPAL DELLVEAPLV LPERGAVEVQ LRIGAPDAAG RRPLEAHARA
     EGGAWTRHAS GSLAADRSTP VPLTEWPPTG AREVDLAGFY DSRAAEGYDY GPALRGLRRA
     WTRDGEVFAE VELPAPLSPD GWGLHPALFD AALHATGLLP PARVAADEVL LPFSWRGTRL
     RATGATALRV RLTGTADDHV LRAYDPEGAP VVEVDALRLR PAPATALVGS GALFRVEWVD
     LDLPVSTPDS TPGAPVLDRP VLDLPGGDAR TLVGLTLEAL REHDELTVVT RGAAEDPAAA
     AVWGLLRTAQ TERPGRFAVV DLDDDPASRA LLPALPATGE PQVRVRAGRA SAPRLARTRD
     DGLPLPAAPT WRLTCDGGTF TDLAATPSPA ANRPLEPGEV RVGVRAAGVN FRDAAVVLNA
     VPGQRGLGGE AAGVVLETGP GVTGLAVGDR GLGTFDRDFG AFGPVAVTDA RLLAPVPPGW
     GFARAASVPV AFLTALYGLR DLAQVGPGDR VLVHAGAGGV GGAAVRLAAL LGAEVFATAS
     PAKWPVLRAL GLDDAHLASS RDTGFAERFP NVDVVLNSLT GELVDASLRL LAPGGRFLEL
     GKVDVRDLVR ADVTYRAYDL RDAGPDRTAE LLAEVLGLFA AGRLEPLPLT VRDVRHAPEA
     LRHVASGGHV GKVVLTVPRP LDPEGTVLVH GGGALGALTA RHLVSAHGAR HLLITSRRGP
     DAPGAAELAA DLAELGATTR FERCDAADRD RVAALLADLG GRPLTAVVHT AGALDDALLA
     DQDAARVDAV LRPKLDGALL LDELTRDADL AAFVLFSSAA GTLGNAGQAN YAAANAALDA
     LAARRRSAGL PATSIAWGHW EHTSALTAGL TAADLARGAR HGAGSLTTTE ALALLDAALA
     SPEPAVLAAA LDPRRAAEVP PLLRDLAGPR RAVAAQAVAG ADRPLIDVVR AHTATVLGHR
     DASAVDANRA FKELGVDSLT AVELRNRLAA ATGLALAATS VFDHPTPALL TAHLLAELGH
     APAAGTTAPP ATAAVTADAG EPIAIVSMAC RFPGDVSSPE DLWDLVAGGG DALTPFPADR
     GWDLAALFAP DPDRAGRSHA REGGFLADVA GFDADLFGIS PREAIGTDPQ QRLLLEVAWE
     LFERAGIAPD SLRGTDTGVF AGVIAHGYPV LPAHSDQGLE GHRVTGVSGS VVSGRVAYAF
     GLQGPAMTVD TACSSSLVGV HLAVRALRGG ECSLALAGGV TVLSTPDLFV DFSRQRGLAA
     DGRCKAFGAG ADGTGFGEGV GLVLLERLWD ARRNGRRVLG LVRGSAVNSD GASNGLTAPS
     GPAQERVIGR ALADAGLSAA DVDLVEAHGT GTRLGDPIEA RALVATYGRD RGGEPVWLGS
     VKSNIGHTQA AAGVAGLIKA VQALRHGVLP ATLHAAEPTP EVEWDGVAVL AEQRDWPEVG
     RPRRAAVSSF GVSGTNAHVV LEQAPEELSP EGRIPEVRIP DRRTSEGRVP EGRAPEGRTS
     GGRAPEGQTS EGRAFGGRVP EDQTSEDRTS EGQASMGRAP EGQAPLGQVS ESRAPDHRVP
     AGPDRDVPGH LPLVLSAHDP AALRATAARL LPVAERVPLP DLALSLVTGR AALPCRAVIP
     ARDRGQALAA LRDLASGATA PAERDGGGVA FLLSGQGSQR AGTGRLLERR FPVFRDALRE
     VCALLDRRVV GGPGVRAALD DPGLLADTRY AQAGLFAVQV ALVRLLDALG VRPDLLAGHS
     VGEIAVAHAA GVLSLEDAST LVAARGALMR ELPPGVMVAV RAGEDEARAA LVDGVELAAV
     NGPRATVLTG DEAAVTEVAA RLGRATRLRV AHAFHSAAVD AVLPAFADVL ARLDFRPPRV
     PVATTAPGAL DAPEYWLGQA REPVRFADAV TDLHRRGATA FVEVGPGGGL AAAAQECLDG
     HDVLCAPLLH RDRDEDHSLI TALGALHARG ARVDFAPLLA DAGGRTTDLP TYPFQRRRFW
     LDPAPPTTDA APLEHPLLTG ELEIPGSDTA AFTGRAATGA HPWLADHRLL DAVVVPGTAL
     LDLVRAAGRQ LGLPEVAELV NEVPLVLGDG AALLRVTAAA PGPDGRREVA VHSRPESAGP
     RSPWTRHVTG QLAPESGPLA FDLAQWPPPG ALPLPVADHR AAMAATGHGY GPAFLGLRAA
     WKLDDEVLVE AVLPDEVADD GHAVHPALLD AVLQAIALTG VEDGSQVRVP FSWRSARGAG
     RAGRAVRASL RPDGDGGVRV RVACPRGRPL LEIASLVSRP VTPDRLRPGV LLRQAWTEVG
     PPPGAAEPVP LREVGERVPP VVRVDAGHVR DLLPLLQEVV TEPRWESTRL LVTTSGAVGP
     RAGRAEGAAV WGLVRSAAAE HPGRFVLADL DDRPESAALL PALATGALPE AVLRAGRVLV
     PGLHRVSAPG AGGAVLDPGG AALITGGSGA LAALLAEHLV RAHGVRCLVL LSRSGRGPDV
     PGADVRHVRG DVANRADVVA ALAAVPPDRP LTLVAHLAGV LDDGALTALT PERLDAVLRP
     KAEGARHLDE LTRDRDLAAF VLFSSVAGAL GHAGQANYAA ANAHLDGLAH ERHALGLPAT
     ALAWGPWTEG MARGRGTLSP QVALGLFDAA LRTGLPALVP LDREPERRVP APRAATPAPA
     GPSLADRLRP MPPAERRAHL LELVTAEALG VLGHDDGAAL PAGQAFRDAG FDSITAMALR
     NRLVRLAGVR LPASVVFDHP TPAATAERLE AELFPADRLP GPRHGDDFDD MAADELVRRA
     LGGE
//