ID   Q8KUH4_ACTPA            Unreviewed;      3073 AA.
AC   Q8KUH4;
DT   01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2002, sequence version 1.
DT   19-JAN-2010, entry version 44.
DE   SubName: Full=Polyketide synthase;
GN   Name=asmB;
OS   Actinosynnema pretiosum subsp. auranticum.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Actinosynnemataceae; Actinosynnema.
OX   NCBI_TaxID=42198;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 31565;
RX   MEDLINE=22056096; PubMed=12060743; DOI=10.1073/pnas.092697199;
RA   Yu T.W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q., Xu J.,
RA   Moss S.J., Leistner E., Floss H.G.;
RT   "The biosynthetic gene cluster of the maytansinoid antitumor agent
RT   ansamitocin from Actinosynnema pretiosum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:7968-7973(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 31565;
RA   Yu T.-W., Bai L., Clade D., Hoffmann D., Toelzer S., Trinh K.Q.,
RA   Xu J., Moss S.J., Leistner E., Floss H.G.;
RL   Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; AF453501; AAM54076.1; -; Genomic_DNA.
DR   HSSP; P25715; 1MLA.
DR   SMR; Q8KUH4; 28-986, 1310-1630, 1758-2630, 2912-2992.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0005044; F:scavenger receptor activity; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR001190; Srcr_rcpt.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 2.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 4.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 2.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00109; ketoacyl-synt; 2.
DR   Pfam; PF02801; Ketoacyl-synt_C; 2.
DR   Pfam; PF00550; PP-binding; 2.
DR   SMART; SM00827; PKS_AT; 2.
DR   SMART; SM00826; PKS_DH; 1.
DR   SMART; SM00822; PKS_KR; 1.
DR   SMART; SM00825; PKS_KS; 2.
DR   SMART; SM00823; PKS_PP; 2.
DR   PROSITE; PS50075; ACP_DOMAIN; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
DR   PROSITE; PS50287; SRCR_2; 1.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   3073 AA;  313428 MW;  66DC2DA015727548 CRC64;
     MTTREQLLDA LRASLRENER LRATGGAAEP VAIVGAACRF PGGVTSPEEL WDLVVGEVDA
     AGPFPGDRGW DLADLFDPDP DRVGRSYVRH GAFLRDMAWF EPGFFGISPR EALVIDPQHR
     LLLEVAWEAF ERAGIAPDSV RGRDVGVFGG VLSHDHGSRF RPVPPELEGY LGTGSSGSAA
     IGRVAYTLGL EGPAITVDTA CSSSLVAIHL AVRALQRGEC SMALAGGVTV MSTPDTFVEF
     SRQRALARDG RCKAFSADAD GTAWGEGAGL VLLQPLSAAR RDGRRVLAVV RGSAVNSDGA
     SNGLTAPSGP AQQRVIRAAL ADAGLSAADV DLLEAHGTGT VLGDPIEAQA LLETYGRGRA
     GAGPLWLGSV KSNIGHTQGA AGVAGVLKVV QALRNGVLPP TLHAATPTTE VDWDAGAVRV
     LDRALPWRGG GEPGSALGDA AGGLEGGAAG GRPGGAAGGV AGGVGGGAAA VRLRRGAVSA
     FGASGTNAHL ILEEAPVAEA VAEAVAPATT GSPAAPGAVG APGVTAAPGT FAATAAVPSA
     PLPAAASDAP VPVPVPAPVP VVVSGATTAG LRAQAARLAG HLRERPALGP EDVARPLLLS
     RAQRERRAVV VARDREALLG GLDALAGGEA GRRLVSGAAD VTGRVVLVFP GQGAHWTGVA
     ERLWREAPAF ADSMARCADV LRDLAGWELR EVLVDPVALE RVDVLQPVSF AVVVSLAALW
     ASVGVRPDAV VGHSQGEVAA AHVAGALTLA EAARIVVLRS ALIARELSGR GAMLTVVADV
     ERVTALLAGF EGRVCVAAVN GPASVTVSGE DAAVREFERV LSARRVLRWR LPGVDFAGHS
     PQVDALRAEL LAALGDVAAR EPEIPLLSTV TGRPATRLDA EHWYRNLREP VRFADAVTAL
     LDRGHRVFVE VSPHPVLTTS VVDLAAPHRT AVVGTLRRDE GGLDRFLLSA GELHVRGVPV
     DLARHAGAGT AEVPTTVFQR ERYWLDAPRP SGDARAAGLV PSGHPLLGAL VPLADGAVLT
     GRLSLSAQPW LADHRVLGAV VVPGVALVEL AVRAGDEVGC PALEELVVAA PLVVPESGDV
     AVRVVVGPRG ADGTRPVDVL SGDQPPVLHA RGVLGPPRAP EPVGGQWPPA GAEPVDLAGL
     YPDFAAKGLE YGPHFQRLTR LWRRGDEVFG EVELTGEQRR AAGFALHPAL LECALHVSSF
     TGQRVAGDGG AVGLPFSFSR VALHRSGVDA VRVRAARVGD GVVSLDLADG AGRPVARIGE
     LAFRRAGGIR SGPPGVLLET AWRPVELPEA PPPADVTTVV GDSVERVLAA LRETLAGDGR
     LLVLTGDPGA PIGTAVGGAV WGLVRSAQLE EPGRIVLADL DTAPERAGAV LDRVLASGEP
     QVAIRGGAVL VPRLTPVPVV AGPAGLDPAK TVVLTGGTGA LGRLVARHLV TEHGARRLLL
     LGRGGPERAR DLVAELTGLG AEVEALACDV ADRGALSTAL AGRALTAVVH LAGALDDGVV
     TALTPERLAA AFAPKALAAR HLDELTRDQD LAAFVLFSSV ASVLGSAGQA NYAAANGFLD
     GLAHERHALG LPATSIAWGL WDVDSALVGE RGAADRERLA ASGMRPLPAP AALDLLDAAL
     ACGRPAVTAL ALARAADRAP AALGEVVASR RAPAPAAPVP GGPAALLALV RAEAAAVLAL
     PVAGVDERRA FRDLGVDSLT GVELRNRLAE ATGLRLPATL VFDHPTPVEL AAALLERVGG
     TSGPAGGASA PAPASAPGEP IAIVGMACRA PGGVASPEDL WDLVAGGVDA VGPFPADRGW
     DLERLVHPDP DHEGSTYVDR AAFLDDPAGF DADFFGISPR EALAMDPQQR LLLEVAWEAF
     ERAGIAPDSV RGTDVGVFAG VTNHDYDQVL PRGDDGYRIT GVSGSVVSGR IAYALGLEGP
     ALSVDTACSA SLVAIHLAVR ALRGGECSMA LAGGATVMAT PRGFVEFARQ RGLAPDGRCK
     PFSASADGTA WSEGAGLVLL ERLSDARRNG RRVLAVVRGS AVNSDGASNG LTAPSGAAQQ
     RVIRAALADA GLSTSDVDAV EAHGTGTRLG DPIEARALVA TYGRDRDGEP LWLGSLKSNL
     GHMQGAAGIG GVVKTVQALR HGVLPRTLHV DAPTPEVEWD GVALLAEQRA WPEVDRPRRA
     AVSSFGVSGT NAHVVLEQAP AERTAAENPG GNAAGRVGDF PGVLPLVLSA RSPAALRAQA
     ARLADVLDDL PLRDVASTLA RGRAAHEHRA VLVAADRDAL RAGLRALAGG RPHAAARTGV
     RAPGGLAFAF SGQGSQFVGM GRGLHAAFPA FAEVFDRVCA ALDERLGELT GRSVRDVVLD
     GPAELLAETV HAQPALFALE VALARLLEGW GVVPDVVLGH SVGELAAAHV AGALSEADAC
     ALVAARARAM HGARRGGAML AVSATEEQVA PLLGGGLALA AVNGPGAVVL SGDGDAVERA
     AAECAGRGWR TRALRVSHAF HSAHLDGVLG ELAVAARRVE VRPPRVPLLS TLTGAPADVE
     LLADPDYWAR QAREAVRFGD GVARLGELGV TRVLEVGPGG ALAALVREQV APGAAVARGL
     VDGDEVVGLL SGIGDLFASG APVDWTALLG GPGAVVDLPT YPFQRTRFWP AAQAPGAWCE
     VRLERLPAPR GGGSATVLEV GPTASAEELA SVADVLAEHS GPLLVVTGDG AVAGLVRSLP
     DRADVVLADV DDDPASVALL PALHGCGEPE LVVRAGEVLA PRLRRVTATG AAPSGPVLVV
     GEAARGVVAA CGVREPVVLP ADSGRAELVA ALDDHRPDWV VLAVAPDDAG LALAALLDEL
     TRDRPPALFA LCGPAGALGD AARPEDAVAA GVLAALVRRR RGDGLPATLL CAGPGTPPLS
     PELGVVVFAR TPEVVLPEPR TAQPEPDAVD LTGPDRDRVL LALVRDHAAA VLGRADGPVD
     GRAAFRDLGF DSMTAVELRD RLAAATGLTL PATLLFDHPT AAEVAAVLAG LLDGGDPVTS
     GLDRLEAALA DPPADPALRA SAAARLRALL AALSDEGDGR DGAGQDGAAA LRDAGVAELF
     EFIDTRLGRA ERA
//