ID   Q93NX8_9ACTO            Unreviewed;      5644 AA.
AC   Q93NX8;
DT   01-DEC-2001, integrated into UniProtKB/TrEMBL.
DT   01-DEC-2001, sequence version 1.
DT   19-JAN-2010, entry version 45.
DE   SubName: Full=AmphJ;
GN   Name=amphJ;
OS   Streptomyces nodosus.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=40318;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=21344785; PubMed=11451671; DOI=10.1016/S1074-5521(01)00046-1;
RA   Caffrey P., Lynch S., Flood E., Finnan S., Oliynyk M.;
RT   "Amphotericin biosynthesis in Streptomyces nodosus: deductions from
RT   analysis of polyketide synthase and late genes.";
RL   Chem. Biol. 8:713-723(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=16079135; DOI=10.1074/jbc.M506689200;
RA   Carmody M., Murphy B., Byrne B., Power P., Rai D., Rawlings B.,
RA   Caffrey P.;
RT   "Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl
RT   groups.";
RL   J. Biol. Chem. 280:34420-34426(2005).
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DR   EMBL; AF357202; AAK73502.1; -; Genomic_DNA.
DR   HSSP; P96202; 1PQW.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR011032; GroES-like.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 3.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 4.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 6.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 3.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   Pfam; PF00109; ketoacyl-synt; 3.
DR   Pfam; PF02801; Ketoacyl-synt_C; 3.
DR   Pfam; PF08659; KR; 2.
DR   Pfam; PF00550; PP-binding; 3.
DR   SMART; SM00827; PKS_AT; 3.
DR   SMART; SM00826; PKS_DH; 3.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00822; PKS_KR; 3.
DR   SMART; SM00825; PKS_KS; 3.
DR   SMART; SM00823; PKS_PP; 3.
DR   PROSITE; PS50075; ACP_DOMAIN; 3.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 3.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   5644 AA;  585795 MW;  CF6A35E3BA0D2658 CRC64;
     MEQTMNAPAE NVVAALRAAV KETERLRRRN RTIVAAAREP IAVVGMGCRF PGGVDSPQAL
     WEMVAGGTDV ISEFPDDRGW DLEALRTSGI DDRDTSVSQR GGFLDSIADF DPGFFGISPR
     EAVTMDPQQR LLLETAWEAI ERARIDATRL RGTRTGTFIG TNGQDYAYLL VRSLDDATGD
     VGTGIAASAV SGRLSYTFGL EGPAITVDTA CSSSLVALHL AVQSLRNGEC TLALAGGVNV
     MSTPGSLVEF SRQGGLAGDG RCKAFSDSAD GTGWSEGAAV LALERLSDAQ RNGHPVLAVI
     RGSAVNQDGA SNGFTAPNGP SQQRVIRQAL SNAGLNPADV DVVEAHGTGT PLGDPIEAQS
     ILATYGQDRE QPLLLGSIKS NIGHTQSAAS GVAGIMKMIM AMRNEVLPKT LHVDRPSTHV
     DWTAGKVELL TENRPWPTAP DRPRRSGVSS FGVSGTNAHV IVEQAPQTPA HQPEEAPADP
     SDEAPAAPRT AGVLPWVLSA RSAAALREQA AALLAHLDAP GAPGALDTGY SLATTRASLE
     HRLAVVTGAD GTAGREALTS WLAGDPAPDA HEGRPVGRTR SAFLFSGQGA QRLGMGRELH
     ARFPVFAEAL DQVLDLLDEE LDASLGDIIW GEEEAPLNET GFTQPALFAV EVALYRLVES
     WGVTPDFVAG HSIGEIAAAH VAGVFSLEDA CRLVAARAGL MQALPSGGAM VAVEATEDEV
     LPLLTEGVAV AAVNGPTSVV VSGEEKATLA VAEQLAAKGR RTSRLRVSHA FHSPLMDPML
     DDFRAVAETL SYDEPQLPVV SNLTGTLAAD GQLTSPEYWV RHVREAVRFA DGVRALAEAG
     ADVLLELGPD GVLAALAQQS ATALTVPFLR KDRPEEHSAV TALARLHTAG VTVDWAAFHD
     GTGARTGELP TYAFQHERYW PKATATAVDA TGLGLASADH PLLGAAMSVA GSDELLLTGT
     LSLATHPWLA DHSVDGMVVF PGTGFLELAV RAADQAGCDR VQELTVATPL VLPATGAVQM
     QISVGAAGED GSRELRFFTR PGEDFDAEWT QHATGRIASG EHVLGFDTTV WPPRDSEAVD
     IEELFDRFAS DGLEYGPVFR GLRAAWRQDD TVYAEVELPD SVEDAGAFGL HPALLHAALH
     GTAFLSDDSG LLPFAWEGVS LHADGASTLR VRIASCGEDT VEIAAVDPAG QPVLSVESLT
     LRAADSGAGA ASRREEANSL FRVDWTPRTV AAPAAPATWA VLGEDPFGLT AALAGDSEAV
     AGVHAPAATL EELAARSGAV PDMVAVTVRG DADGGPEAAR ELTREVLALV QGWLAEPAFA
     SSRLVVVTRD AVADGERGAV DLAAAPVWGL VRSAQSENPG RLLLADVDDT ADSLARLPLL
     AGLFDAEEPQ AVVREGTVRV GRLARLESGD SSARALDPEG TVLVTGGTGG LASALARHLV
     AEHDIRHLLL TSRRGPDAPG AADLVQALAE LGAEARVAAC DVADREALAA LLASVPAEHP
     LTAVVHTAGV LDDGIFPSLT PDRLDSVMRP KVDAAWHLHD LTRDLDLAAF VLYSSTAGVM
     GSPGQANYAA GNTFLDALAA HRQSLGLPAT SLAWGAWEQG VGMTGQLSGQ DARRISDAGG
     MPLLSVERGL ALYDAAMLAD EPLVVPLGLG GGGPLPAGAG VPAILRGLVR TGGRRARAAT
     AAVARAGLAE RLAVLPEEQR RPFVVDLVRA EAAAVLGHGS ADAVDSRREF RGLGFDSLTA
     IELRNRLGKA TGLTLPATLV FDYPTPEQLA DHLLDELLGA DAIEVFAASQ SAADVHDDPV
     VIVGMGCRFP GGVGSPEDLW DLLASGSDAI TGFPADREWE SSRLVAGEAG GVSAQGGFLS
     DIAGFDADFF GISPREALAM DPQQRILLEV TWEAIERAGV DPTALRGSRT GVFMGVNGQD
     YSSLVMGSRD DVAGHATAGL AVSVVSGRLS YALGLEGPAL SVDTACSSSL VSLHLAAQAL
     RSGECSMALV GGVTVMTTPA NFAGFSRMGG LAQDGRCKAF SDSADGTGWS EGAAVLVVER
     LSDARRAGHR VLAVVRGSAV NQDGASNGLT APNGPSQQRV IRQALANAGL RPGDVDAVEA
     HGTGTPPGDP IEAQALLATY GSDRDPQQPL LLGSVKSNIG HTQAAAGVAG LVKMVMAMRN
     GVLPRTLHIT EPSTHVDWSL GAVQVLTEET AWPETGRVRR AGVSSFGISG TNAHVILEGA
     PDEPVPAPVA DRPVPGAVAW PVSAKSEGAL DDQAERLRES ADALPALDTA YTLATGRADF
     EHRAVLLAAD GTLTEVARGV AEPHRSAFLF SGQGAQRLGM GRELHARFPV FAEAFDSVTA
     LLESELDTSV REVMWGTDEG ALNATAFTQP ALFAVEVALY RLVESWGVTP DFVAGHSVGE
     IAAAHVAGVF SLEDACRLVA ARAGLMQALP SGGAMVAVEA TEDEVLPLLT EGVAVAAVNG
     PTSVVVSGEE QATLAVAEQL AAQGRRTSRL RVSHAFHSPL MDPMLEDFRA VAETLSYHEP
     RIPVVSNLTG EVAAAGVHTH PDYWVRHVRE AVRFADGVRG LADRGVTALL EIGPDGVLSA
     LAAASLTDTD TVVVPALRKD RDETVSVLSG VARLYVAGVD VDWSAPLSGA GARIADVPTY
     AFQHERYWPK AAPAALDATG LGLASADHPL LGAAMSVAGS DELLLTGSLS AATHPWLADH
     VVGGMIFFPG TGFLELAVRA ADQAGCDRVE ELMIAAPLVL PATGAVQVQI SVGAADEEGS
     RELRFFTRPG EDFDAEWTQH ATGRIGSGEQ VIDFDATVWP PRDAEAIDID GMFERYAADG
     LEYGPVFRGL RAVWRQDDTV YAEVALPESV EDADAFGLHP ALFDAALHST VFLSAEGDTR
     SLLPFAWEGV SLHADGASTL RVRIASCGED TVQIAAVDPG GQPVVSVESL TLRAAGPGDA
     AEPRRDDSNS LLRVDWTART LGAPAAPATW AVLGEDPFGL TAALAGDSEA VAGVHAPAAT
     IEELAARSGA VPDMVAVTVR GDADAGPDDA HELAHEVLAL VQGWLAEPAF ASSRLVVVTR
     NAVADGERGA VDLAAAPVWG LVRSAQSENP GRLLLADVDD TADSLARLPL LAGLFDAEEP
     QAVVREGTVR VGRLARLESG TSLVPPAGTP WRLGCRAKGS LDGLALLPYP EAVTPLTGRE
     VRIGIRAAGL NFRDVLNALG MYPGEAGLFG SEGAGVVSEV GPDVTGLAPG DRVMGMVFGG
     FGPLGVADER LLTRVPDDWS WETASSVPLV FLTAYHALKD LAGLRPGEKI LIHAGAGGVG
     MAAIQIAHHL GAEVFATASE GKWDVLRSLG VADDHIASSR TLDFETAFTE VAGDKGLDVV
     LNALAGEFVD ASMRLLGTGG RFLEMGKTDI RDSDAASDGI TYRFFDLGMV DPDHIQQMLL
     DLVDLFERDV LSPLPVRAWD VRRSREAFRF MSMAKHIGKI VLTMPRAMDP EGTVLVTGGT
     GGLASALARH LVAEHGIKHL LLTSRRGPDA PGAADLVQAL AELGAEARVA ACDVADRDAL
     AGLLASVPAE RPLTAVVHTA GVLDDGILAS LTPDRLDTVM RPKVDAAWHL HDLTRDLDLA
     AFVLYSSTSG VFGSPGQANY AAGNTFLDAL AAHRQSLGLP ATSLAWNAWE QGSGMTSGLS
     DEDMRRINDN SGMPLLSVER GLALYDAATL ADEPLVVPLG LGGGGSLPPG MSVPAILRGL
     VRTGGRRAKA GAAAVARAGL AERLAVLPEE QRLPFVVDLV RAEAATVLGH GSADAVDARR
     EFRGLGFDSL TAIELRNRLG KASGLTLTAT LVFDYPTPQQ LAEHLLDELL GADAAEAFAA
     PQTAAAASDD DPVVIVGMGC RFPGGVGSPE ELWDLVASGT DAITGFPADR EWESSTIGGE
     PGDLSGQGGF LSDIADFDAD FFGIAPREAL AMDPQQRILL EVTWEAVERA GLDPTALRGS
     RTGVFMGVSG QDYSGLVMRS RDDIASHATT GLAVSVVSGR LSYTLGLEGP ALSVDTACSS
     SLVSLHLAAQ ALRSGECTMA LAGGVTVMTT PANFTGFSKM GGLAHDGRCK AFSDSADGTG
     WSEGAAVLVL ERLSDARRAG HRVLAVVRGS AVNQDGASNG LTAPNGPSQQ RVIRQALANA
     GLRPGDVDAV EAHGTGTPLG DPIEAQALIA TYGSDRDPQQ PLLLGSVKSN IGHTQSAAGA
     AGLVKMVMAM HQGTLPRTLH VTEPSTHVDW SLGAVRLLTE ETAWPETGRV RRAGVSSFGI
     SGTNAHVILE GAPEPTADDR SAEDTVTPAV TPWVVSARSE QALDAQLERL RAHAAAHPEL
     SGADIGLSLV TSRPSFEHRA VLLAGPDGIT EAARAEAGTA RTPAFLFSGQ GAQRLGMGRE
     LHARFPVFAE VFDSVTALLE SELGTSVREV MWGTDEAALN STAFTQPALF AVEVALYRLV
     ESWGVTPDFV AGHSVGEIAA AHVAGVFSLE DACRLVAARA RLMDALPRGG AMAAVEATED
     EVLPLLDDGV AVAAVNGPTS VVVSGPEDGV DQLVALLESD GRRTTRLRVS HAFHSSLMDP
     MLEDFRAVAE TLSYHEPRIP VVSNLTGEVA SAGTHTHPDY WVRHVREAVR FADGVRALAD
     RGVTAFLEIG PDGVLSALAA ASLPDTGTVV VPALRKDRDE TVSVLTALAR LHTAGLDTDW
     SAHFAGTGAR TVELPTYAFQ GTRFWPDTTA APGDAGGLGL DAGGHPLLTA ATSVAGSDET
     LLSGRLSAAA QPWLTGRTEN GTTILPTAVL AELALHAAEA CDRTTVENLT VGAPLALTGN
     RPQRLQVLVG VPDETGRRTL TVHTRADGDD APWVERATAM LTDAPAAATP DTVWPPADAT
     PVDELPEPTG PSVLRAAWRR GGDVFAEVEI TEQSPAEQAF ALHPALLDTA VRAAVLLEGD
     GGGSGDDTLD AVAWDGLVLH AAHPVLLRVR LTATGDDTWA LEATDPQGGP VLSVASVTLG
     ATVAAPVTGA PATDDAALLA LDWVAPAPAP RSGDSGPWTV LGDALPGLDT ALAAVDNVLV
     TRADSLAELL DSGAPLPSLM LLPVEGGPTA GHDLPAAVRA ATTRVLDLLR RWTSDPRTAD
     SRLAIVTRGA VAAGREDVTD LAAAAVWGLV RSAQSENPGC FLLLDLDPAD AAEATDAAVL
     ASLPALFDAG ETQAAVRGGA LTVARLTRTE ATPAPAADQV RAWDRDGTVL ITGGTGGLGA
     VLARHLVTGH GIKHLLLAGR RGPDAPGATA LSKELSALGA EVTVRACDVS DRSAVDALLA
     GLPAEHPLTA VVHTAGVLDD ATIGTLTAEQ LDTVLRPKAD AAWHLHQATR ALPLAGFVLY
     SSVAGVTGGP GQGNYAAANT FLDALAAHRA AQGLPALSLA WGPWGQGAGM TGTLSDADLE
     RMERSGMPPL TERQGLALFD AANGHDEALA VAIRVSRSAA APDAGEVPAV LRSLVRARRR
     AAATAGADGL TRRLAGLGAE QRHETLVGLV RQETAGVLGH SGADAVPADR DFSRLGFDSL
     MAVELRTRLS AATGVRLPST LVFDHPTPAA VARHLADSLT GQDRSGTAAS PLAALDRLEA
     ELSADGVDEA VRRGVEGRLR RLLAAWDGTG SDGNGPAVEE RIEAASAEEI FAFIDNELGR
     SSDS
//