ID   Q9L4W4_9ACTO            Unreviewed;      3192 AA.
AC   Q9L4W4;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   19-JAN-2010, entry version 55.
DE   SubName: Full=NysB;
GN   Name=nysB;
OS   Streptomyces noursei.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1971;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 11455;
RX   MEDLINE=20334850; PubMed=10873841; DOI=10.1016/S1074-5521(00)00120-4;
RA   Brautaset T., Sekurova O.N., Sletta H., Ellingsen T.E., StrLm A.R.,
RA   Valla S., Zotchev S.B.;
RT   "Biosynthesis of the polyene antifungal antibiotic nystatin in
RT   Streptomyces noursei ATCC 11455: analysis of the gene cluster and
RT   deduction of the biosynthetic pathway.";
RL   Chem. Biol. 7:395-403(2000).
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DR   EMBL; AF263912; AAF71775.1; -; Genomic_DNA.
DR   HSSP; P43677; 1NQ4.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR015083; Polyketide_synth_docking.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 2.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 2.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 4.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 2.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF08990; Docking; 1.
DR   Pfam; PF00109; ketoacyl-synt; 2.
DR   Pfam; PF02801; Ketoacyl-synt_C; 2.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF00550; PP-binding; 2.
DR   SMART; SM00827; PKS_AT; 2.
DR   SMART; SM00822; PKS_KR; 2.
DR   SMART; SM00825; PKS_KS; 2.
DR   SMART; SM00823; PKS_PP; 2.
DR   PROSITE; PS50075; ACP_DOMAIN; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   3192 AA;  333922 MW;  F88F8864F086DA64 CRC64;
     MQEPQQGQPD QQEKIVDYLR RVTSDLRRAR RRIGELESKD NEPIAIVGMG CRLPGGVNSP
     ESLWDLVRSG GDAISGFPVD RGWDLETLTG NGDGSSATHE GGFLYDAAEF DAAFFGISPR
     EATAMDPQQR LLLEVAWEAL ERAGIAPTAL RGSRSGVFVG SYHWGAPSAD AATELHGHAL
     TGTAASVLSG RLAYTLGLEG PAVTVDTACS SSLVALHLAA QSLRVGESSL AVIGGVTILT
     EPSVFVEFSA QGGLAPDGRC KAFSDAADGT GWAEGVGVLV AERLSDAQRN GHPVLAVLRG
     SAVNQDGASN GLTAPNGPSQ ERVIQQALAR TGLTPADIDA VEAHGTGTRL GDPIEAQALL
     ATYGQGHTPD QPLWLGSLKS NIGHTQAAAG VAGVIKMVMA LRHGHLPPTL HADAPSSHVD
     WSAGSVRLLT EGQQWPETGR PRRAAVSSFG ISGTNAHALL EQAPHPADTA DAGDDAAPTE
     PAGAPAALPW IVSGHSPQAL RDQAAALAAR VETDPALRPQ DIGHTLHTAR ALLERRAVVV
     APDRAELLAA THELAAGRSA NAVVEGLADV EGRTVFVFPG QGSQWVGMGA QLLDESAVFA
     ERIAECAAAL AEFTDWSLVD VLRGVVGAPS LERVDVVQPA SFAVMVSLAA LWGSRGVLPD
     AVVGHSQGEI AAAVVSGALS LRDGARVVAL RSQAIGRALA GRGGMMSVAL SVDVLEPRLV
     EFEGRVSVAA VNGPRSVVVA GEPEALDALH ARLTADDIRA RRIAVDYASH SHQVEDLHEE
     LLEVLAELAP RTSEVPFFST VTGDWLDTAR MDAGYWFRNL RGRVRFADAV ADLLAAEYRA
     FVEVSSHPVL TMAVLDLIEE AGVTAVATGT LRRDQGGAGR FLLSAAEVFV RGVDVDWAGA
     FEGTGAARVD LPTYAFQRER YWNTRTAADR TPADAPMDAE FWAAVEQADV SALTAALGTD
     EDSVAAILPG LTSWRRARSQ RTTLDSWRYR VTWTPLAQVP RATLTGTWLL VTTDGIDDTD
     VAGALESYGA EVRRLVLDEE CTDRAVLRER LAGAEDVTGI VSVLAAAEDD AARHPGLTRG
     LALTVSLVQA LGDAEATAPL WFLTRGAFAT GPSDPVTRPL QSQIAGVGWT TALEHPQRWG
     GTVDLPDTLD ARAAQRLAAA LSGALGAEDQ LAVRAAGVLA RRIVRAGHRA GRPARTWAPR
     GTTLITGGSG TLAPQLARWL AERGAEHVVL VSRRGADAPG APELIAEAAE SGTEVTVAAC
     DITDRDAVAA LLADLTADGR TLRTVIHAAA AIELSALADT TVAEFADVVH AKVTGARILD
     ELLDDAELDD FVLYSSTAGM WGSGVHAAYV AGNAYLSALA EQRRARGLRT TSIHWGKWPD
     DRARELADPH RIRRSGLEYL DPELALTALQ HVLDDDETVI GLMDIDWDTY HDVFTAGRPA
     HLFDQIPEVR RRLDQASVPD PAGPAADGLA ARLHGLAAAE QDRLLLTLVR TEAAAVLGHA
     SAESFPERRA FRDLGFDSVT AVDLRNRLVA GTGLRLPSTM VFDHPNCAAL AAFLKTTALG
     VPGAAPQQHA ATGTPADDDP IAVIGMSCRY PGGAATPEEL LRLALDGADV ISEFPADRGW
     DARGLYDPDP DRPGHTYSVQ GGFLHEAAGF DPGFFGISPR EAVAMDPQQR LLLETSWEAF
     ERAGIDPASL RGSAAGTFFG ASYQDYSSTV QNGTGESEAH MVTGTAASVL SGRVSYLLGL
     EGPAVTVDTA CSSSLVALHL ACQSLRDGES SLALAGGAAV MATPHAFVGF SRQRALAKDG
     RCKPFSDTAD GMTLAEGVGV VLLERLSHAR ANGHRVLAVI RGSAVNQDGA SNGLTAPNGP
     SQQRVIRQAL ANAGLTGADV DAVEAHGTGT KLGDPIEAQA LLATYGQDRD AERPLLLGSV
     KSNIGHTQAA AGVAGVIKMV LAMDAGELPG TLHLDAPSSH VDWTAGAVEL LRGRTPWPES
     GRPRRAGVSS FGISGTNAHL ILEQAPATEP PADPDRLRDT ATDTVVPWPL AAKSPAALRA
     QAARLLATVE HDPDLPPAPV GHALATTRAA LEHRAVVVGE RREDFLRGLA ALSTGASTAG
     LVSGIAGPDP EGAVFVFPGQ GSQWWGMGRE LLATSEVFRT AIDDCATALA PYVDWSLHDV
     LAGEGDPALL ERVDVVQPAL FAMMVGLSAL WRSHGVVPAA VVGHSQGEIA AACVAGALSL
     ADAARVVALR SQALPQLSGR GGMMSVSAPV ERVTALLAPW QEALSVAAVN GPSSVVVSGD
     TDALDALHTA CQEQGVRARK VSVDYASHGR HVEAVRDELA RVLAPVDPRA PEVPFYSTVT
     GDRVDDAAFD GAYWYTNLRQ TVRMEEATRA LLAAGHRVFI EVSPHPVLAA PIQETQEAVA
     EATGGSAVVL GSLRRDEGGP RRFLTSLAEA HTHGAPVDWT TTFARSAYQP VDLPTYPFQR
     QDFWPEARPA TPAAGADASD AAFWQLVENQ DLAALADALG VPADDEHTAL GTVLPALSAW
     RAKAQARTRI DELRYHVQWT RVAEPAAAPT TGRLLVAVPP DHADAPWVAA ALDALGTDTV
     RFEAKGTDRA GWAAQIAQLV EDGEEFTGVV SLLAAAEDLH PDFGSVPLGL GQTLVLVQAL
     GDAGLTAPLW CLTRGAVATG RDDALDSPTQ GALWGLGRVV ALEHPDRWGG LIDLPATLDA
     RAAARLTGLL ADPAGEDQLA VRATGVLARR MVHAAPSAPR TGRRWRGRGT CLITGGTGGI
     GGRVARWMAE HGAAHLVLTS RRGPDAPGAA ALRAELEALG ARVTLAACDV ADRDALAALL
     ADLPADQPLT SVFHSAGVAD GDARAADLTL DQLDALLRAK LTAAHHLHEL TAPLDLDAFV
     LFSSGAAVWG SGGQPGYAAA NAYLDALAAH RRSLDLPGAS VAWGTWGEVG MATVPEVHER
     LHRQGVRAME PDHAIGALQQ MLEDDDTTLA VTLMDWEAFA PSFTATRPSA LFSTVPEAVR
     AVTGDPGTTA GDDVDSATPP LRRHLEELSA AERGRALVEA VRAEASATLG HDTPDAIPAG
     RAFRDVGFDS VTAVELRNRL RTALGLPLPA ALVFDHPTPT ALAGHLGALL FGTAPEDAGT
     GRPDDPDARI REALATVPIG RLRKAGLLDM VLKLADGDAT DAPAPEADAP SESLDDMDAE
     ALLRLATENS AN
//