ID   Q9L4X1_9ACTO            Unreviewed;      2066 AA.
AC   Q9L4X1;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   19-JAN-2010, entry version 56.
DE   SubName: Full=NysK;
GN   Name=nysK;
OS   Streptomyces noursei.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1971;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 11455;
RX   MEDLINE=20334850; PubMed=10873841; DOI=10.1016/S1074-5521(00)00120-4;
RA   Brautaset T., Sekurova O.N., Sletta H., Ellingsen T.E., StrLm A.R.,
RA   Valla S., Zotchev S.B.;
RT   "Biosynthesis of the polyene antifungal antibiotic nystatin in
RT   Streptomyces noursei ATCC 11455: analysis of the gene cluster and
RT   deduction of the biosynthetic pathway.";
RL   Chem. Biol. 7:395-403(2000).
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DR   EMBL; AF263912; AAF71768.1; -; Genomic_DNA.
DR   HSSP; P25715; 1MLA.
DR   SMR; Q9L4X1; 4-910, 948-993, 1301-1647, 1412-1779, 1793-2059.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR020802; PKS_thioesterase.
DR   InterPro; IPR015083; Polyketide_synth_docking.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR001031; Thioesterase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 2.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF08990; Docking; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   Pfam; PF00975; Thioesterase; 1.
DR   SMART; SM00827; PKS_AT; 1.
DR   SMART; SM00826; PKS_DH; 1.
DR   SMART; SM00822; PKS_KR; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SMART; SM00823; PKS_PP; 1.
DR   SMART; SM00824; PKS_TE; 1.
DR   PROSITE; PS50075; ACP_DOMAIN; 1.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   2066 AA;  214573 MW;  CB02F5991FB2B56E CRC64;
     MPDEKKLVDY LKWVTKDLHQ TRQRLQEVEA GRHEPVAIVG MACRFPGGVR SPEDLWELLS
     AGRDGIGPFP ADRGWDLAAL AGDGPGRSAT QEGGFLPDAA AFDPGFFDIS PREALAMDPQ
     QRLLLETAWE AVERSGIDPA GLRGSRTGVF VGTNGQDYAH LVLAAQDDMG GYAGNGLAAS
     VLSGRLAFAL GLEGPAVTLD TACSSSLVTL HLAAQAVRAG ECGLALAGGV TVMTTSSSFA
     GFSLQGGLAP DGRCKAFAEA ADGTGWSEGI GLLLVERLSD AQRNGHPVLA VLRGSAVNQD
     GASNGLSAPN GPSQQRVIRQ ALAGAGLVPG DVDAVEAHGT GTRLGDPIEA GALLATYGQD
     RPADRPLWLG SVKSNLGHTQ AAAGVAGVIK MVLALRHGVL PQTLHVDAPS SHVDWESGAV
     RLLTAPVAWS EGDDRVRRAG VSSFGISGTN AHVILEQAPD QPEPTAEETA AAAPGGTAEE
     RAAAPVAPRA VPWPVAARTA GALDAQLVRV RALTTAPGRT AADVGHALAT ARTPFEHRAL
     LVHEGGAVTE VARGAVPTGD RGGLAVLFSG QGSQRPGMGR ELHARYPVFA AAFDETVALL
     DARLGTSLRD IVWDQDRTRL DDTRHTQPAL FAVEVALYRL LASWGIRPDH VTGHSIGEIT
     AAHVAGVLTL ADACTLVAAR ATAMSELPPG GAMVALEATE DEVRPLLTDD LAIAAVNAPR
     SVVVAGAEDA ALAVRRHFDD LGRRTTRLPV SHAFHSPLMD PMLDAFRTAL APLTFAEPEI
     PVVSNLTGLP ATAEELATPH YWVCHVRQAV RFGDGVRALA DRGVRTFLEL GPDGVLSALV
     RENLPEPGLV AVPVLRKERP EETTVLAALG TLWAHGADVD WDAVFAGTRT PQADPVELPT
     YAFQRARYWP TLGARHGDPA DLGQTAAAHP LLGAAVTLAD ADETVLTGRL ALPSHPWLGD
     HRSDGRITVP GVAFAELAVR AGDLSGTPHL ARLDLPAPLT LGDGDTVTLQ VRVGAPDPAG
     HRPLTVHARL AATEDAPWTT CATGLLAPDA PEAPADPIGP ADAGWPPRDA RPVPVADLDA
     AATAAGRHYG PHFQGLTGLW RRDGEVFAEV ALPTATAADR AFGIHPALLA TALRATAALD
     DDHTAGHTPE PTGITGLALH ATGATALRVR LTATGPDTVA LAAADATGGA VLTADTVTLG
     SPQDRPAPAP AGHTGQGGLF HLDWVPVDPG SRATGTRWAV VGDDELDLGY ALHRADETVS
     AYAASLGGAI GDSGLAPDVF LVPVVGGPDA GPDAVHAVTA RALGLLQEWL NEPRLAGARL
     VFVTRGAVAV PGETVTDPAG AAVWGLLRSA QTENPGSLLL VDLDDAFRSA GMLPHVLTLD
     EQQLVVRDHA VRAARLARLP EPAAGTAPAR AWDPDGTVLI TGGTGGLGAA LARHLVTVRG
     ARHLLLAGRR GPEAPGAGEL VAELTAQGAD VRVAACDVGD RTALDALLAT VPAAHPLTAV
     VHTAGVLDDA LIGSLTPDQL ATVLRPKADA AWHLHDATRG LDLAGFVLYS SVSGVLGSPG
     QGNYAAANAY LDALARHRAD QGLPALSLAW GPWGRGSGMT ASVSDADLER MARGGLPPLT
     VEDGLALFDA AVGRPEPALV PSRINVAGLR DQQALPALWR DLVPRARRTA ATADRSPVTV
     RERLRHLDET GQEQLLIDLV VGYTAGLLGH PDPTAVDPER GFLELGFDSL VSVGLRNQLA
     EILGLRLPSS IVFDSKSPVK LARWLHQELA NGPQPGATGP AAADARPAVR SDDTLEGLFY
     NAVRGGKLVE AMRMLKAVAN TRPMFDTPAE LEELSEPVTL ADGPGRPRLI FVSAPGATGG
     VHQYARIAAH FRGSRHVSAL PLMGFAPGEL LPATSEAAAR IVAESVLMAS EGEPFVMVGH
     STGGSLAYLA AGVLEDTWDV RPEAVVLLDT ASIRYNPGEG NDLDRTTRFY LADIDSPSVT
     LNSARMSAMA HWFMAMTDIQ APAPTAPTLL VRAARALDGF RLDTSSVPAD EVRDIDADHL
     SLAKEHSALT AQAIEGWLAE LPDPAA
//