ID   Q9L4X2_9ACTO            Unreviewed;      5435 AA.
AC   Q9L4X2;
DT   01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2000, sequence version 1.
DT   19-JAN-2010, entry version 48.
DE   SubName: Full=NysJ;
GN   Name=nysJ;
OS   Streptomyces noursei.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1971;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 11455;
RX   MEDLINE=20334850; PubMed=10873841; DOI=10.1016/S1074-5521(00)00120-4;
RA   Brautaset T., Sekurova O.N., Sletta H., Ellingsen T.E., StrLm A.R.,
RA   Valla S., Zotchev S.B.;
RT   "Biosynthesis of the polyene antifungal antibiotic nystatin in
RT   Streptomyces noursei ATCC 11455: analysis of the gene cluster and
RT   deduction of the biosynthetic pathway.";
RL   Chem. Biol. 7:395-403(2000).
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DR   EMBL; AF263912; AAF71767.1; -; Genomic_DNA.
DR   HSSP; P96202; 1PQW.
DR   GO; GO:0000036; F:acyl carrier activity; IEA:InterPro.
DR   GO; GO:0048037; F:cofactor binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR001227; Ac_transferase_dom.
DR   InterPro; IPR009081; Acyl_carrier_prot-like.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPlipase.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR011032; GroES-like.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006163; Phsphopanteth_bd.
DR   InterPro; IPR020842; PKS/FAS_KR.
DR   InterPro; IPR020801; PKS_acyl_transferase.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_reg.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR006162; PPantetheine_attach_site.
DR   InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR016038; Thiolase-like_subgr.
DR   Gene3D; G3DSA:3.40.366.10; Ac_transferase_reg; 3.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 4.
DR   Gene3D; G3DSA:3.40.47.10; Thiolase-like_subgr; 6.
DR   PANTHER; PTHR11712; Ketoacyl_synth; 1.
DR   Pfam; PF00698; Acyl_transf_1; 3.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   Pfam; PF00109; ketoacyl-synt; 3.
DR   Pfam; PF02801; Ketoacyl-synt_C; 3.
DR   Pfam; PF08659; KR; 2.
DR   Pfam; PF00550; PP-binding; 3.
DR   SMART; SM00827; PKS_AT; 3.
DR   SMART; SM00826; PKS_DH; 2.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00822; PKS_KR; 3.
DR   SMART; SM00825; PKS_KS; 3.
DR   SMART; SM00823; PKS_PP; 3.
DR   PROSITE; PS50075; ACP_DOMAIN; 3.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 3.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
DR   PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE   4: Predicted;
KW   Phosphopantetheine; Transferase.
SQ   SEQUENCE   5435 AA;  562677 MW;  AA55465DF087A38C CRC64;
     MNAPENPETP ENNVVAALRA AVKETDRLRR QNRMLVAAAK EPIAVVGMAC RFPGAVDSPE
     ALWEMVATGT DVISGFPDDR GWDLEALRNS GTDARDTDVS QRGGFLDCIA DFDPGFFGIS
     PREAVTMDPQ QRLLLTTAWE AVERAGIDAT TLRATRTGAF IGTNGQDYAY LLVRSLDDAT
     GDVGTGIAAS AASGRLSYTL GLEGPALTVD TACSSSLVAL HLAVQALRNG ECGMALAGGV
     NVMATPGSLV EFSRQGGLAR DGRCKAFADA ADGTGWSEGA GVLLLERLSD AQRNGHPVLA
     VVRGSAVNQD GASNGFTAPN GPSQQRVIRQ ALANAGLATG DIDAVEAHGT GTPLGDPIEA
     QSILATYGQD RAHPVLLGSI KSNMGHTQAA SGVAGVIKMI MAMRHGVLPR TLHVDRPSTH
     VDWTTGSVEL LTDAHPWPET GRPRRTGISS FGVSGTNAHV IVEQAPDTPA EAADDTPPRT
     PRTLPWLLSA RTGAALRDQA TALLDHLDRP DGDRGPTALD TAFSLATTRA ALEHRLAVVT
     GTDGTAGRDA LTAWLAHGTA PDAHEGHAAG RTRCAALFSG QGAQRLGMGR ELHARFPVFA
     RALDTAVDLL DAELGGTLRE VIWGTDDAPL NETGFTQPAL FAVEVALYRL IESWGVAPDF
     VAGHSIGEIA AAHVAGVFSL EDACTLVAAR AGLMQALPRG GAMVAVEATE DEVSPLLTDG
     VAIAAINGPT SLVVSGDETA TLAVAARLAE QGRRTTRLRV SHAFHSPLMD PMLAEFRAVA
     EGLSYGEPQI PVVSNLTGAV ADGTLLGTAD YWVRHVREAV RFADGIRALT DAGVGAFLEL
     GPDGTLAALA QQSAPDAVSV PVLRKDRDEE PAAVAALARL HTAGVPVDWT AFYAGTGAHR
     TDLPTYAFQY ERYWPKATYR PADATGLGLT AADHPLLGAA MSVAGSDELL LTGTLSLATH
     PWLADHVVGG MVFFPGTGFL ELAVRAADQV GCDRVEELML AAPLILPATG TVQMQIAVGA
     ADDDGGRDLR FFTRPGDDPD AAWAQHATGR ITEGERVLAL DTTTWPPRDA EPVDIDGLYD
     RYRANGLDYG PVFRGLRAVW RRDTEIYAEV ALPEGTADAD AFGLHPALFD AVLHSTLFAS
     ADGDDRSLLP FAWNGVSLHA AGADALRVRI TSCGPDAVEI TAVDPQGRPV VSVESLTLRA
     AGPDAGTADH RADAGSLFRM DWTPRTVHAP ATPATWAVLG TDPIGLTEAL TAAGPDTVTG
     LRDGVDALGE LTAGDDRPVP DVVAVPLRGA TDHGPAGAHD LTRTVLALLQ EWLAEERFAR
     SRLLLVTRGA VADGERGPLD LAAAPVWGLV RSAQSENPGR LLLVDLDDTA ESAAQLPLLP
     ALLDADEPQA VVREGTVRVG RLARLDSGRG LVPPPGTPWR LGSRAKGSLD GLALLPHPEA
     RRPLTGHEVR VGIRAAGLNF RDVLNALGMY PGDAGLFGSE AAGVVVEVGP EVTGLAPGDR
     VMGMLFGGFG PLGIADARLL TPVPADWSWE TGASVPLVFL TAYYALKELG GLRAGEKVLV
     HAGAGGVGMA AIQIARHVGA EVFATASEGK WDVLRSLGVA DDHIASSRTL DFEAAFAEVA
     GDRGLDVVLN ALSGEFVDAS MRLLGDGGRF LEMGKTDIRA ADSVPDGLSY HSFDLGMVDP
     EHIQRMLLDL VELFDRGALA ALPVRSWDVR RAGEAFRFMS LAQHIGKIVL TVPQPLDPDG
     TVLLTGGTGG LAGLLARHLV TEHGARHLLL AGRRGPDAPG AAALHAELTA LGAEVTVAAC
     DVADRTALAA LLATVPAEHP LTAVVHTAGV LDDGTLTALN PDRLATVLRP KVDAAWHLHD
     LTRHLDLAAF VLYSSTAGVM GGPGQANYAA GNTFLDALAA HRHALGLPAT SLAWGAWEQG
     AGMTGALTDH DLRRVSDAGG QPLLTAERGL ALYDAATAAD EPLIVPLGLT GGALPAGVGV
     PAVLRGLVRT AGRRARAGTA GVSRAGLAER LAALPEEERT PFLVELVRTE AATVLGHGST
     DPVDARREFR QLGFDSLTAI ELRNRLGKAT GLTLPATLIF DYPTPDRLAV HLHDELLGAD
     APVTVTAAAQ AADPEHDPVV IVGMSCRFPG GVSSPEELWD LVASGTDAIT GFPADRAWDR
     HPQLAGAPGA RTGQGGFLRD IADFDAAFFG ISPREALAMD PQQRILLEVA WEAAERAGID
     PQTLRGSDTG VFMGVSGQDY AGLVMRSRDD IAGHATTGLA VSVVSGRLAY ALGLEGPALS
     VDTACSSSLV SLHLAAQALR AGECTMALAG GVTVMTTAAN FTGFSRMGGL AQDGRCKAFS
     DSADGTGWSE GAAVLVLERL SDARRAGHRV LAVVRGSAVN QDGASNGLTA PNGPAQQRVI
     RQALANAGLT PVDVDAVEAH GTGTPLGDPI EAQALIAAYG TDRDPEHPLL LGSVKSNIGH
     TQSAAGAAGL VKMVMAMRHG ILPQTLHLTE PSSHVDWSAG TVRLLTERTA WPRTDRPRRA
     GVSSFGISGT NAHVILEQPP AEPTPAADPG RPAPTVVAWP VSAQTPAALD AQLDRLRTAA
     ALAPLDTAHT LATGRSLFEH RAVLLATVGD PATGAPDLPE VARGAATPHR TAFLFSGQGA
     QRSGMGRELH AAFPVFAAAF DEVVAVLDAE LGSDADGGVS LREVMWGGGS ELLDRTRFTQ
     PALFAVEVAL FRLVASWGVG PEFVAGHSVG EIAAAHVAGV FSLVDACRLV VARASLMDAL
     PVGGVMVAVE AAEAEVVPLL VDGVAIAAVN GPVSVVVSGV EAAVGQVVDQ LVERGRRVRR
     LAVSHAFHSP LMDPMLDAFR AVAEGLEYHQ PRIPVVSNVT GEVAAAEELC AADYWVRHVR
     ATVRFADGVR TLAERGATAF LEIGPDGVLS ALARGVLPAE ALVTPTLRKD RDEESALLAG
     LARLHVAGVT VDWSAALTGT GARGTDLPTY AFQRERYWPE LAAEPAGGGA DAADAEFWAA
     VERADATALA AHLDIDGDQL GAVLPALSAW RTRRRTTSAT NALRHRESWE PLSLAGTPHT
     GGVLVLVPAA ATTDPWVADV VAALGPDARR VDVPADGTDR AALAALLTEA ADDTAPTAVV
     SLLALDETSG DDAVPAGTTA TAALVQALAD TGAPAPLWAL TRGAVAALPD EQPTAPAQAA
     VWGLGRIAAL ELPRHWGGLV DLPADLDERT ARRLPAALAD AGDEDQLALR ATGAYGRRIT
     PAPAPDDAPG TGWQPTGTVL ITGGTGALGR HTARWLAAHG AEHLLLLSRS GPDAPGAAEL
     TTELTALGAR VTLVACDAAD REQLTRVLAE VPRDCPLTGV VHTAGVLDDG VLTGLTPDRF
     ATVFRAKVAS AVLLDELTRD TDLAVFALFS SVAGAVGNPG QAGYAAANAV LDALAARRRA
     QGLAGTSIAW GAWAGDGMAA RHTRPGAEPV GLLDPDLAVP ALARAVTEPQ PTLVLADLQQ
     PRLLESLLAL RPSPLLSRLP AARTAARAVQ EADRRRAGAA ADLRDQLAGT APADRHAVLL
     RLVRTTAAAV LGHTGADAIR ADKPFRDLGF DSLTAVELSS ALAAATGLAL PPSLVFDHPS
     PRALADHLRA ELTGDRPESA PAAPPAPVPA ADDDPIVVVG MACRFPGGVT TPEEFWQLLA
     EGRDGIDAFP TDRGWDLDVL GRRRPGPQRP PRSAASSYDA AAFDPGFFDI SPREALAMDP
     QQRLLLETAW EAVERTGTDP TRLRGSRTGV FVGTNGQDYA GLVLRAQEDV EGHAGTGLAA
     SVISGRLAYA FGFEGPAVTV DTACSSSLVA LHWAVQALRA GECSLALAGG VTVMTTSTSF
     AGFTRQGGLA PDGHCKAFSD SADGTGWSEG VGVLVVERRS DALRNGHEIL AVVRGSAVNQ
     DGASNGLTAP NGPAQQRVIR QALANAGLAP GDVDAVEAHG TGTVLGDPIE AQALLATYGQ
     DRPADRPLWL GSVKSNIGHT QAAAGAAGLM KMVLALQHGT LPRTLHVTEP STRVDWSAGA
     VRLLTERTVW PRTDRPRRAG VSSFGISGTN AHVILEQPPA EPTPTAPADR PTRTPAVLPW
     VVSARSATAL DAQLARLRAF AAERPDLPPA DVAHSLVTSR ATFEHRAVLL AAPDGITAAA
     RAEARERSTA FLFSGQGAQR SGMGRELHAA FPVFAAAFDE VVAVLDAELA TGSGGGVSLR
     EVMWGGGSEL LDRTRFTQPA LFAVEVALFR LVASWGVGPE FVAGHSVGEI AAAYVAGVFS
     LVDACRLVVA RASLMDALPV GGVMVAVEAA EAEVVPLLVD GVAIAAVNGP VSVVVSGVEA
     AVGQVVDQLV ERGRRVRRLA VSHAFHSPLM DPMLDAFRAV AEGLEYHQPR IPVVSNVTGE
     VAAAEELCAA DYWVRHVRAT VRFADGVRTL AERGATAFLE IGPDGVLSAL AAACLFDTDA
     EVVPALRKGR PEEHTALTAA AQLHVAGVDI DWTAVLAGTG GRRIALPTYA FQRERYWPSL
     AAQAPGDAGG LGLEAGRHPL LGAATTVAGS AEILLTGRLS TTAQPWLAVY EADGRTVLPA
     AVLAELAVRA GDQADCPTVA ELTVAAPLVL TGAAAQRLQV RVAAPDDTGR RALSVHARPD
     DSPDSPWTLH ATAVLTHDTP QPPAPDTGWP PERAVPLDAL PTATGPARIA AAWQWGDELC
     AEIELPEPGP AERAFALHPA LLDTAVRAGG LLDGDATLDA LGWRGLALHA ASATALRVRL
     TPDGTDTWAL EATDPQGAPV VSVTGLTLGT PTVDRSGAGA ADDGATLLDL EWVPAPQAAP
     TGGDHLPYAV LGDQLAELDG QLRIAGDGPG RVASLAALLD GGAPLPRLVL APVLGVPTGE
     GDLPAAVRGT TTAVLELLQR WTADARTADS HLVIVTRGAV AAGAEDVHDL AAAPVWGLVR
     SAQSEHPGSF LLLDLDPADP AGASRAAAPA TLAALLDAGE TQAAVRADTL TVARLTRAAD
     GPEATAGHPV RDWDRDGTVL ITGGTGGLGG LLARHLVTGH GIKHLLLAGR RGPDAPGARA
     LRDELAALGA EVTVAACDVA DRAALDRLLA QLPPEHPLTA VVHTAGVLDD ATVGTLTPER
     LDTVLRAKAD AAWHLHDATR DRDLAGFVLY SSVAGVTGGP GQGNYAAGNT FLDALAAHRA
     AQGLPGLSLA WGPWGQDAGM TGTLGAADLA RLERSGMPPL TPEQGLALFD AAGARGDGFA
     VAVRLARGAA APGADEVPAV LRALVRGRRR TAAAAGHAGV LARRLAALDA EQRHQALLDL
     VRTETAAVLG HSGADAVPAE RDFNRLGFDS LMAVELRTRL ATATGARLPA TLVFDHPTPD
     AVARHLASTL PGGTAAGPDR SPLAELDRIA AELSPEGADD ATRQGVVGRL RHLLAQWDGT
     RQDGGGTTVD DRIEAASAEE VLAFIDHELG RQADS
//