KEGG   ENZYME: 1.14.12.9
Entry
EC 1.14.12.9                Enzyme                                 

Name
4-chlorophenylacetate 3,4-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)
Reaction(IUBMB)
4-chlorophenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + chloride + NAD+ [RN:R03306]
Reaction(KEGG)
R03306
Substrate
4-chlorophenylacetate [CPD:C03077];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxyphenylacetate [CPD:C01161];
chloride [CPD:C00698];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase and an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+. Also acts on 4-bromophenyl acetate.
History
EC 1.14.12.9 created 1989 as EC 1.13.99.4, transferred 1992 to EC 1.14.12.9
Pathway
ec00350  Tyrosine metabolism
ec01120  Microbial metabolism in diverse environments
Reference
1  [PMID:3745216]
  Authors
Markus A, Krekel D, Lingens F.
  Title
Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS.
  Journal
J Biol Chem 261:12883-8 (1986)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.9
IUBMB Enzyme Nomenclature: 1.14.12.9
ExPASy - ENZYME nomenclature database: 1.14.12.9
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.9
BRENDA, the Enzyme Database: 1.14.12.9
CAS: 105006-00-6

KEGG   REACTION: R03306
Entry
R03306                      Reaction                               

Name
4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)
Definition
4-Chlorophenylacetate + Oxygen + NADH + H+ <=> 3,4-Dihydroxyphenylacetate + Hydrochloric acid + NAD+
Equation
Reaction class
RC00001  C00003_C00004
RC00901  C01161_C03077
Enzyme
Pathway
rn00350  Tyrosine metabolism
rn01120  Microbial metabolism in diverse environments
Other DBs
RHEA: 14692

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