KEGG   ENZYME: 1.14.20.13
Entry
EC 1.14.20.13               Enzyme                                 

Name
6beta-hydroxyhyoscyamine epoxidase;
hydroxyhyoscyamine dioxygenase;
(6S)-6-hydroxyhyoscyamine,2-oxoglutarate oxidoreductase (epoxide-forming)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
Sysname
(6S)-6beta-hydroxyhyoscyamine,2-oxoglutarate:oxygen oxidoreductase (epoxide-forming)
Reaction(IUBMB)
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 = scopolamine + succinate + CO2 + H2O [RN:R03737]
Reaction(KEGG)
R03737
Substrate
(6S)-6beta-hydroxyhyoscyamine;
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
scopolamine [CPD:C01851];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
Requires Fe2+ and ascorbate.
History
EC 1.14.20.13 created 1992 as EC 1.14.11.14, transferred 2018 to EC 1.14.20.13
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Reference
1
  Authors
Hashimoto, T., Kohno, J. and Yamada, Y.
  Title
6beta-Hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger.
  Journal
Phytochemistry 28:1077-1082 (1989)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.20.13
IUBMB Enzyme Nomenclature: 1.14.20.13
ExPASy - ENZYME nomenclature database: 1.14.20.13
BRENDA, the Enzyme Database: 1.14.20.13
CAS: 121479-53-6

KEGG   REACTION: R03737
Entry
R03737                      Reaction                               

Name
(6S)-6beta-hydroxyhyoscyamine,2-oxoglutarate:oxygen oxidoreductase (epoxide-forming)
Definition
(6S)-Hydroxyhyoscyamine + 2-Oxoglutarate + Oxygen <=> Scopolamine + Succinate + CO2 + H2O
Equation
Reaction class
RC00989  C01851_C03325
Enzyme
Pathway
rn00960  Tropane, piperidine and pyridine alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Other DBs
RHEA: 12800

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