Entry |
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Name |
GDP-mannose 4,6-dehydratase;
guanosine 5'-diphosphate-D-mannose oxidoreductase;
guanosine diphosphomannose oxidoreductase;
guanosine diphosphomannose 4,6-dehydratase;
GDP-D-mannose dehydratase;
GDP-D-mannose 4,6-dehydratase;
Gmd;
GDP-mannose 4,6-hydro-lyase;
GDP-mannose 4,6-hydro-lyase (GDP-4-dehydro-6-deoxy-D-mannose-forming)
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Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
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Sysname |
GDP-alpha-D-mannose 4,6-hydro-lyase (GDP-4-dehydro-alpha-D-rhamnose-forming)
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Reaction(IUBMB) |
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O [RN: R00888]
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Reaction(KEGG) |
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Substrate |
GDP-alpha-D-mannose [CPD: C00096]
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Product |
GDP-4-dehydro-alpha-D-rhamnose [CPD: C01222];
H2O [CPD: C00001]
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Comment |
The bacterial enzyme requires bound NAD+. This enzyme forms the first step in the biosynthesis of GDP-alpha-D-rhamnose and GDP-beta-L-fucose. In Aneurinibacillus thermoaerophilus L420-91T, this enzyme acts as a bifunctional enzyme, catalysing the above reaction as well as the reaction catalysed by EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase [5]. Belongs to the short-chain dehydrogenase/reductase enzyme family, having homologous structures and a conserved catalytic triad of Lys, Tyr and Ser/Thr residues [6].
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History |
EC 4.2.1.47 created 1972, modified 2004
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Pathway |
ec00051 | Fructose and mannose metabolism |
ec00520 | Amino sugar and nucleotide sugar metabolism |
ec00541 | O-Antigen nucleotide sugar biosynthesis |
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Orthology |
K01711 | GDPmannose 4,6-dehydratase |
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Genes |
» show all
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Reference |
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Authors |
ELBEIN AD, HEATH EC. |
Title |
THE BIOSYNTHESIS OF CELL WALL LIPOPOLYSACCHARIDE IN ESCHERICHIA COLI. II. GUANOSINE DIPHOSPHATE 4-KETO-6-DEOXY-D-MANNOSE, AN INTERMEDIATE IN THE BIOSYNTHESIS OF GUANOSINE DIPHOSPHATE COLITOSE. |
Journal |
J Biol Chem 240:1926-31 (1965) |
Reference |
|
Authors |
Liao TH, Barber GA. |
Title |
Purification of guanosine 5'-diphosphate D-mannose oxidoreductase from Phaseolus vulgaris. |
Journal |
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Reference |
|
Authors |
Melo A, Elliott WH, Glaser L. |
Title |
The mechanism of 6-deoxyhexose synthesis. I. Intramolecular hydrogen transfer catalyzed by deoxythymidine diphosphate D-glucose oxidoreductase. |
Journal |
J Biol Chem 243:1467-74 (1968) |
Reference |
|
Authors |
Sullivan FX, Kumar R, Kriz R, Stahl M, Xu GY, Rouse J, Chang XJ, Boodhoo A, Potvin B, Cumming DA. |
Title |
Molecular cloning of human GDP-mannose 4,6-dehydratase and reconstitution of GDP-fucose biosynthesis in vitro. |
Journal |
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Sequence |
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Reference |
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Authors |
Kneidinger B, Graninger M, Adam G, Puchberger M, Kosma P, Zayni S, Messner P |
Title |
Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T. |
Journal |
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Reference |
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Authors |
Mulichak AM, Bonin CP, Reiter WD, Garavito RM. |
Title |
Structure of the MUR1 GDP-mannose 4,6-dehydratase from Arabidopsis thaliana: implications for ligand binding and specificity. |
Journal |
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Sequence |
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Other DBs |
ExplorEnz - The Enzyme Database: | 4.2.1.47 |
ExPASy - ENZYME nomenclature database: | 4.2.1.47 |
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