Entry |
|
Name |
mannuronan 5-epimerase;
algG (gene name);
alginate epimerase;
C5-mannuronan epimerase;
mannuronan C-5-epimerase
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Class |
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
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Sysname |
[mannuronan]-beta-D-mannuronate 5-epimerase
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Reaction(IUBMB) |
[mannuronan]-beta-D-mannuronate = [alginate]-alpha-L-guluronate
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Reaction(KEGG) |
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Substrate |
[mannuronan]-beta-D-mannuronate
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Product |
[alginate]-alpha-L-guluronate
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Comment |
The enzyme epimerizes the C-5 bond in some beta-D-mannuronate residues in mannuronan, converting them to alpha-L-guluronate residues, and thus modifying the mannuronan into alginate. It is found in brown algae and alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
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History |
EC 5.1.3.37 created 2015
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Pathway |
ec00051 | Fructose and mannose metabolism |
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Orthology |
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Genes |
» show all
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Reference |
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Authors |
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE |
Title |
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. |
Journal |
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Sequence |
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Reference |
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Authors |
Morea A, Mathee K, Franklin MJ, Giacomini A, O'Regan M, Ohman DE |
Title |
Characterization of algG encoding C5-epimerase in the alginate biosynthetic gene cluster of Pseudomonas fluorescens. |
Journal |
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Reference |
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Authors |
Nyvall P, Corre E, Boisset C, Barbeyron T, Rousvoal S, Scornet D, Kloareg B, Boyen C |
Title |
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata. |
Journal |
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Reference |
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Authors |
Jain S, Franklin MJ, Ertesvag H, Valla S, Ohman DE |
Title |
The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa. |
Journal |
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Reference |
|
Authors |
Douthit SA, Dlakic M, Ohman DE, Franklin MJ |
Title |
Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix. |
Journal |
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Reference |
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Authors |
Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codee JD, Klassen JS, Howell PL |
Title |
Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG. |
Journal |
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Sequence |
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Other DBs |
ExplorEnz - The Enzyme Database: | 5.1.3.37 |
ExPASy - ENZYME nomenclature database: | 5.1.3.37 |
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