| Entry |
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| Name |
L-rhamnose isomerase;
rhamnose isomerase;
L-rhamnose ketol-isomerase
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| Class |
Isomerases;
Intramolecular oxidoreductases;
Interconverting aldoses and ketoses, and related compounds
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| Sysname |
L-rhamnose aldose-ketose-isomerase
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| Reaction(IUBMB) |
L-rhamnopyranose = L-rhamnulose [RN: R02437]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Contains two divalent metal ions located at different metal-binding sites within the active site. The enzyme binds the closed ring form of the substrate and catalyses ring opening to generate a form of open-chain conformation that is coordinated to one of the metal sites. Isomerization proceeds via a hydride-shift mechanism. While the enzyme from the bacterium Escherichia coli is specific for L-rhamnose, the enzyme from the bacterium Pseudomonas stutzeri has broad substrate specificity and catalyses the interconversion of L-mannose and L-fructose, L-lyxose and L-xylulose, D-ribose and D-ribulose, and D-allose and D-psicose [2].
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| History |
EC 5.3.1.14 created 1965
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| Pathway |
| ec00051 | Fructose and mannose metabolism |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K01820 | L-rhamnose isomerase / sugar isomerase |
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| Genes |
» show all
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| Reference |
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| Authors |
DOMAGK GF, ZECH R. |
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| Title |
[ON THE DECOMPOSITION OF DESOXY SUGARS BY BACTERIAL ENZYMES. I. L-RHAMNOSE ISOMERASE FROM LACTOBACILLUS PLANTARUM.] |
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| Journal |
Biochem Z 339:145-53 (1963) |
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| Reference |
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| Authors |
Leang K, Takada G, Ishimura A, Okita M, Izumori K |
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| Title |
Cloning, nucleotide sequence, and overexpression of the L-rhamnose isomerase gene from Pseudomonas stutzeri in Escherichia coli. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Korndorfer IP, Fessner WD, Matthews BW |
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| Title |
The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Yoshida H, Yamada M, Ohyama Y, Takada G, Izumori K, Kamitori S |
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| Title |
The structures of L-rhamnose isomerase from Pseudomonas stutzeri in complexes with L-rhamnose and D-allose provide insights into broad substrate specificity. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 5.3.1.14 |
| ExPASy - ENZYME nomenclature database: | 5.3.1.14 |
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