| Entry |
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| Name |
xylose isomerase;
D-xylose isomerase;
D-xylose ketoisomerase;
D-xylose ketol-isomerase
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| Class |
Isomerases;
Intramolecular oxidoreductases;
Interconverting aldoses and ketoses, and related compounds
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| Sysname |
D-xylose aldose-ketose-isomerase
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| Reaction(IUBMB) |
D-xylopyranose = D-xylulose [RN: R01432]
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| Reaction(KEGG) |
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| Substrate |
D-xylopyranose
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| Product |
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| Comment |
Contains two divalent metal ions, preferably magnesium, located at different metal-binding sites within the active site. The enzyme catalyses the interconversion of aldose and ketose sugars with broad substrate specificity. The enzyme binds the closed form of its sugar substrate (in the case of glucose, only the alpha anomer) and catalyses ring opening to generate a form of open-chain conformation that is coordinated to one of the metal sites. Isomerization proceeds via a hydride-shift mechanism.
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| History |
EC 5.3.1.5 created 1961 (EC 5.3.1.18 created 1972, part incorporated 1978)
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| Pathway |
| ec00040 | Pentose and glucuronate interconversions |
| ec00051 | Fructose and mannose metabolism |
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| Orthology |
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| Genes |
» show all
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| Reference |
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| Authors |
HOCHSTER RM, WATSON RW. |
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| Title |
Enzymatic isomerization of D-xylose to D-xylulose. |
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| Journal |
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| Reference |
2 |
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| Authors |
Slein, M.W. |
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| Title |
Xylose isomerase from Pasteurella pestis, strain A-1122. |
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| Journal |
J Am Chem Soc 77:1663-1667 (1955) |
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| Reference |
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| Authors |
Yamanaka K. |
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| Title |
Purification, crystallization and properties of the D-xylose isomerase from Lactobacillus brevis. |
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| Journal |
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| Reference |
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| Authors |
Carrell HL, Glusker JP, Burger V, Manfre F, Tritsch D, Biellmann JF |
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| Title |
X-ray analysis of D-xylose isomerase at 1.9 A: native enzyme in complex with substrate and with a mechanism-designed inactivator. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Collyer CA, Blow DM |
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| Title |
Observations of reaction intermediates and the mechanism of aldose-ketose interconversion by D-xylose isomerase. |
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| Journal |
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| Reference |
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| Authors |
Whitlow M, Howard AJ, Finzel BC, Poulos TL, Winborne E, Gilliland GL |
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| Title |
A metal-mediated hydride shift mechanism for xylose isomerase based on the 1.6 A Streptomyces rubiginosus structures with xylitol and D-xylose. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Allen KN, Lavie A, Glasfeld A, Tanada TN, Gerrity DP, Carlson SC, Farber GK, Petsko GA, Ringe D |
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| Title |
Role of the divalent metal ion in sugar binding, ring opening, and isomerization by D-xylose isomerase: replacement of a catalytic metal by an amino acid. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 5.3.1.5 |
| ExPASy - ENZYME nomenclature database: | 5.3.1.5 |
| BRENDA, the Enzyme Database: | 5.3.1.5 |
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