| Entry |
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| Name |
pyruvate dehydrogenase (quinone);
pyruvate dehydrogenase (ambiguous);
pyruvic dehydrogenase (ambiguous);
pyruvic (cytochrome b1) dehydrogenase (incorrect);
pyruvate:ubiquinone-8-oxidoreductase;
pyruvate oxidase (ambiguous);
pyruvate dehydrogenase (cytochrome) (incorrect)
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| Class |
Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With a quinone or similar compound as acceptor
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| Sysname |
pyruvate:ubiquinone oxidoreductase
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| Reaction(IUBMB) |
pyruvate + ubiquinone + H2O = acetate + CO2 + ubiquinol
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Flavoprotein (FAD) [1]. This bacterial enzyme is located on the inner surface of the cytoplasmic membrane and coupled to the respiratory chain via ubiquinone [2,3]. Does not accept menaquinone. Activity is greatly enhanced by lipids [4,5,6]. Requires thiamine diphosphate [7]. The enzyme can also form acetoin [8].
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| History |
EC 1.2.5.1 created 2010
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| Pathway |
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| Orthology |
| K00156 | pyruvate dehydrogenase (quinone) |
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| Genes |
» show all
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| Reference |
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| Authors |
Recny MA, Hager LP |
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| Title |
Reconstitution of native Escherichia coli pyruvate oxidase from apoenzyme monomers and FAD. |
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| Journal |
J Biol Chem 257:12878-86 (1982) |
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| Sequence |
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| Reference |
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| Authors |
Cunningham CC, Hager LP |
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| Title |
Reactivation of the lipid-depleted pyruvate oxidase system from Escherichia coli with cell envelope neutral lipids. |
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| Journal |
J Biol Chem 250:7139-46 (1975) |
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| Reference |
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| Authors |
Koland JG, Miller MJ, Gennis RB |
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| Title |
Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase. |
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| Journal |
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| Reference |
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| Authors |
Grabau C, Cronan JE Jr |
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| Title |
In vivo function of Escherichia coli pyruvate oxidase specifically requires a functional lipid binding site. |
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| Journal |
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| Reference |
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| Authors |
Wang AY, Chang YY, Cronan JE Jr |
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| Title |
Role of the tetrameric structure of Escherichia coli pyruvate oxidase in enzyme activation and lipid binding. |
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| Journal |
J Biol Chem 266:10959-66 (1991) |
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| Reference |
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| Authors |
Chang YY, Cronan JE Jr |
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| Title |
Sulfhydryl chemistry detects three conformations of the lipid binding region of Escherichia coli pyruvate oxidase. |
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| Journal |
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| Reference |
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| Authors |
O'Brien TA, Schrock HL, Russell P, Blake R 2nd, Gennis RB |
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| Title |
Preparation of Escherichia coli pyruvate oxidase utilizing a thiamine pyrophosphate affinity column. |
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| Journal |
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| Reference |
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| Authors |
Bertagnolli BL, Hager LP |
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| Title |
Role of flavin in acetoin production by two bacterial pyruvate oxidases. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 1.2.5.1 |
| ExPASy - ENZYME nomenclature database: | 1.2.5.1 |
| BRENDA, the Enzyme Database: | 1.2.5.1 |
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