KEGG   ORTHOLOGY: K00227Help
Entry
K00227                      KO                                     

Name
SC5DL, ERG3
Definition
Delta7-sterol 5-desaturase [EC:1.14.19.20]
Pathway
ko00100  Steroid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01130  Biosynthesis of antibiotics
Module
M00101  Cholesterol biosynthesis, squalene 2,3-epoxide => cholesterol
M00102  Ergocalciferol biosynthesis
Disease
H01281  Lathosterolosis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K00227  SC5DL, ERG3; Delta7-sterol 5-desaturase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.20  Delta7-sterol 5(6)-desaturase
     K00227  SC5DL, ERG3; Delta7-sterol 5-desaturase
BRITE hierarchy
Other DBs
RN: R07215 R07486 R07491 R07505
GO: 0050046
Genes
HSA: 6309(SC5D)
PTR: 451624(SC5D)
PPS: 100979606(SC5D)
GGO: 101151866(SC5D)
PON: 100459805(SC5D)
NLE: 100604476(SC5D)
MCC: 706827(SC5D)
MCF: 101867112(SC5D)
CSAB: 103248668(SC5D)
RRO: 104657234(SC5D)
RBB: 108523218(SC5D)
CJC: 100387284(SC5D)
SBQ: 101038292(SC5D)
MMU: 235293(Sc5d)
MCAL: 110301807(Sc5d)
MPAH: 110327921(Sc5d) 110334695
RNO: 114100(Sc5d)
MUN: 110540918(Sc5d)
CGE: 100752960(Sc5d)
NGI: 103727913(Sc5d)
HGL: 101713479(Sc5d)
CCAN: 109698547(Sc5d)
OCU: 100358493(SC5D)
TUP: 102481940(SC5D)
CFA: 610411(SC5D)
VVP: 112920309(SC5D)
AML: 100467645(SC5D)
UMR: 103662293(SC5D)
UAH: 113259940(SC5D)
ORO: 101367504(SC5D)
ELK: 111153897
FCA: 101099997(SC5D)
PTG: 102956406(SC5D)
PPAD: 109278494(SC5D)
AJU: 106976671(SC5D)
BTA: 525154(SC5D)
BOM: 102283021(SC5D)
BIU: 109569702(SC5D)
BBUB: 102394495(SC5D)
CHX: 102180515(SC5D)
OAS: 101109313 101117697(SC5D)
SSC: 100520925(SC5D)
CFR: 102520592(SC5D)
CDK: 105097554(SC5D)
BACU: 103004682(SC5D)
LVE: 103083016(SC5D)
OOR: 101282178(SC5D)
DLE: 111167467(SC5D)
PCAD: 102994174(SC5D)
ECB: 100063543(SC5D)
EPZ: 103543812(SC5D)
EAI: 106842373(SC5D)
MYB: 102246367(SC5D)
MYD: 102754805(SC5D)
MNA: 107534112(SC5D)
HAI: 109395291(SC5D)
DRO: 112301615 112301887(SC5D)
PALE: 102891666(SC5D)
RAY: 107519049(SC5D)
MJV: 108389489 108409205(SC5D)
LAV: 100661853(SC5D)
TMU: 101351645
MDO: 100030841(SC5D)
SHR: 100932949(SC5D)
PCW: 110207244(SC5D)
OAA: 100073392(SC5D)
GGA: 100857756(SC5D)
MGP: 100538497(SC5D)
CJO: 107324219(SC5D)
NMEL: 110387590(SC5D)
APLA: 101803446(SC5D)
ACYG: 106039809(SC5D)
TGU: 100226384(SC5D)
LSR: 110477544(SC5D)
SCAN: 103823051(SC5D)
GFR: 102043549(SC5D)
FAB: 101811093(SC5D)
PHI: 102109366(SC5D)
PMAJ: 107214298(SC5D)
CCAE: 111939317(SC5D)
CCW: 104692196(SC5D)
ETL: 114066789(SC5D) 114072326
FPG: 101920444(SC5D)
FCH: 102057522(SC5D)
CLV: 102095349(SC5D)
EGZ: 104125957(SC5D)
NNI: 104016662(SC5D)
ACUN: 113488442(SC5D)
PADL: 103916253(SC5D)
AAM: 106490218(SC5D) 106491711
ASN: 102385911(SC5D)
AMJ: 102571716(SC5D)
PSS: 102463426(SC5D)
CMY: 102931181(SC5D)
CPIC: 101952975(SC5D)
ACS: 100566753(sc5d)
PVT: 110078414(SC5D)
PBI: 103051481(SC5D)
PMUR: 107285543(SC5D)
TSR: 106551450(SC5D)
PMUA: 114585161(SC5D)
GJA: 107125348(SC5D)
XLA: 108697607(sc5d.S) 447026(sc5d.L)
XTR: 100490845(sc5d)
NPR: 108797036(SC5D)
DRE: 447891(sc5d)
SANH: 107703513
IPU: 100528129(sc5d)
PHYP: 113541626(sc5d)
AMEX: 103032395(sc5d)
EEE: 113574240(sc5d)
TRU: 101067117(sc5d)
LCO: 104921101(sc5d) 104938430
ONL: 100695064(sc5d) 100696185
OLA: 101173647(sc5d)
XMA: 102219081(sc5d)
XCO: 114153517(sc5d)
PRET: 103476328(sc5d)
CVG: 107081853(sc5d)
NFU: 107387205(sc5d)
KMR: 108238026(sc5d)
CSEM: 103395378(sc5d)
POV: 109624686 109635651(sc5d)
LCF: 108891924(sc5d) 108901922
SDU: 111225164 111233090(sc5d)
SLAL: 111656091(sc5d) 111666108
HCQ: 109515690(sc5d) 109526775
MALB: 109959324(sc5d)
SASA: 100195087(sc5d) 106576971
OTW: 112253002 112257983(sc5d)
SALP: 111952019 111980773(sc5d)
ELS: 105023255 105024195(sc5d)
SFM: 108936096(sc5d)
PKI: 111844810(sc5d)
LCM: 102365779(SC5D)
CMK: 103190397(sc5d)
RTP: 109938738(sc5d)
SPU: 588561
SKO: 100375039
NVL: 108559476
BMOR: 101737328
BTAB: 109032888
FCD: 110862568
CSCU: 111625209
PCAN: 112567998
MYI: 110461770
PDAM: 113668803
SPIS: 111331638
DGT: 114523409
AQU: 100635449
ATH: AT3G02580(STE1) AT3G02590
CPAP: 110807494
CIT: 102630557
TCC: 18602021
GRA: 105788590
GAB: 108489860
EGR: 104422113
VRA: 106765343
VAR: 108345518
VUN: 114181961
CCAJ: 109791074
CAM: 101511716
LJA: Lj0g3v0103419.1(Lj0g3v0103419.1)
FVE: 101292472
RCN: 112174786
PPER: 18783760
PMUM: 103342673
PAVI: 110763174
CSV: 101204738
CMO: 103487532
MCHA: 111016898
RCU: 8261209
JCU: 105638767
CANN: 107860069
SIND: 105177929
OSA: 4325687
DOSA: Os01t0134500-01(Os01g0134500)
OBR: 102707173
BDI: 100823877
ATS: 109738537(LOC109738537)
SBI: 8061354
SITA: 101780951
DCT: 110108874
PEQ: 110026229
ATR: 18432187
PPP: 112288804
MNG: MNEG_2125
APRO: F751_6009
SCE: YLR056W(ERG3)
ERC: Ecym_1501
KMX: KLMA_20308(ERG3)
NDI: NDAI_0C06430(NDAI0C06430)
TPF: TPHA_0D00440(TPHA0D00440) TPHA_0P00250(TPHA0P00250)
TBL: TBLA_0D02520(TBLA0D02520)
TDL: TDEL_0C00670(TDEL0C00670)
KAF: KAFR_0D01010(KAFR0D01010)
PIC: PICST_28450(ERG3)
SPAA: SPAPADRAFT_48108(ERG3)
CAL: CAALFM_C104770CA(ERG3)
CDU: CD36_04520(ERG3)
SLB: AWJ20_2868(ERG3)
NTE: NEUTE1DRAFT116882(NEUTE1DRAFT_116882) NEUTE1DRAFT78153(NEUTE1DRAFT_78153)
MGR: MGG_08832
SSCK: SPSK_02588
BFU: BCIN_16g04720(Bcerg3)
MBE: MBM_05730
ANG: ANI_1_28134(An15g00150) ANI_1_406144(An16g02930)
PTE: PTT_15444
ZTR: MYCGRDRAFT_74506(ERG3)
SPO: SPAC1687.16c(erg31) SPBC27B12.03c(erg32)
CNE: CNA05010
CNB: CNBA4830
LBC: LACBIDRAFT_190564(ERG3)
ABP: AGABI1DRAFT59529(AGABI1DRAFT_59529)
ABV: AGABI2DRAFT218236(AGABI2DRAFT_218236)
MGL: MGL_0204
MRT: MRET_1111
DFA: DFA_02096
SMIN: v1.2.034753.t1(symbB.v1.2.034753.t1) v1.2.037901.t1(symbB.v1.2.037901.t1)
HCH: HCH_01597
 » show all
TaxonomyKoalaUniProt
Reference
PMID:8976377
  Authors
Matsushima M, Inazawa J, Takahashi E, Suzumori K, Nakamura Y
  Title
Molecular cloning and mapping of a human cDNA (SC5DL) encoding a protein homologous to fungal sterol-C5-desaturase.
  Journal
Cytogenet Cell Genet 74:252-4 (1996)
DOI:10.1159/000134427
  Sequence
[hsa:6309]
Reference
PMID:1864507
  Authors
Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M
  Title
Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.
  Journal
Gene 102:39-44 (1991)
DOI:10.1016/0378-1119(91)90535-J
  Sequence
[sce:YLR056W]

KEGG   ENZYME: 1.14.19.20Help
Entry
EC 1.14.19.20               Enzyme                                 

Name
Delta7-sterol 5(6)-desaturase;
lathosterol oxidase;
Delta7-sterol Delta5-dehydrogenase;
Delta7-sterol 5-desaturase;
Delta7-sterol-C5(6)-desaturase;
5-DES;
SC5DL (gene name);
ERG3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
BRITE hierarchy
Sysname
Delta7-sterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating
Reaction(IUBMB)
a Delta7-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a Delta5,7-sterol + 2 ferricytochrome b5 + 2 H2O
Reaction(KEGG)
Substrate
Delta7-sterol;
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
Delta5,7-sterol;
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
This enzyme, found in eukaryotic organisms, catalyses the introduction of a double bond between the C5 and C6 carbons of the B ring of Delta7-sterols, to yield the corresponding Delta5,7-sterols. The enzymes from yeast, plants and vertebrates act on avenasterol, episterol, and lathosterol, respectively. The enzyme is located at the endoplasmic reticulum and is membrane bound.
History
EC 1.14.19.20 created 1972 as EC 1.3.3.2, transferred 2005 to EC 1.14.21.6, transferred 2015 to EC 1.14.19.20
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K00227  Delta7-sterol 5-desaturase
Genes
HSA: 6309(SC5D)
PTR: 451624(SC5D)
PPS: 100979606(SC5D)
GGO: 101151866(SC5D)
PON: 100459805(SC5D)
NLE: 100604476(SC5D)
MCC: 706827(SC5D)
MCF: 101867112(SC5D)
CSAB: 103248668(SC5D)
RRO: 104657234(SC5D)
RBB: 108523218(SC5D)
CJC: 100387284(SC5D)
SBQ: 101038292(SC5D)
MMU: 235293(Sc5d)
MCAL: 110301807(Sc5d)
MPAH: 110327921(Sc5d) 110334695
RNO: 114100(Sc5d)
MUN: 110540918(Sc5d)
CGE: 100752960(Sc5d)
NGI: 103727913(Sc5d)
HGL: 101713479(Sc5d)
CCAN: 109698547(Sc5d)
OCU: 100358493(SC5D)
TUP: 102481940(SC5D)
CFA: 610411(SC5D)
VVP: 112920309(SC5D)
AML: 100467645(SC5D)
UMR: 103662293(SC5D)
UAH: 113259940(SC5D)
ORO: 101367504(SC5D)
ELK: 111153897
FCA: 101099997(SC5D)
PTG: 102956406(SC5D)
PPAD: 109278494(SC5D)
AJU: 106976671(SC5D)
BTA: 525154(SC5D)
BOM: 102283021(SC5D)
BIU: 109569702(SC5D)
BBUB: 102394495(SC5D)
CHX: 102180515(SC5D)
OAS: 101109313 101117697(SC5D)
SSC: 100520925(SC5D)
CFR: 102520592(SC5D)
CDK: 105097554(SC5D)
BACU: 103004682(SC5D)
LVE: 103083016(SC5D)
OOR: 101282178(SC5D)
DLE: 111167467(SC5D)
PCAD: 102994174(SC5D)
ECB: 100063543(SC5D)
EPZ: 103543812(SC5D)
EAI: 106842373(SC5D)
MYB: 102246367(SC5D)
MYD: 102754805(SC5D)
MNA: 107534112(SC5D)
HAI: 109395291(SC5D)
DRO: 112301615 112301887(SC5D)
PALE: 102891666(SC5D)
RAY: 107519049(SC5D)
MJV: 108389489 108409205(SC5D)
LAV: 100661853(SC5D)
TMU: 101351645
MDO: 100030841(SC5D)
SHR: 100932949(SC5D)
PCW: 110207244(SC5D)
OAA: 100073392(SC5D)
GGA: 100857756(SC5D)
MGP: 100538497(SC5D)
CJO: 107324219(SC5D)
NMEL: 110387590(SC5D)
APLA: 101803446(SC5D)
ACYG: 106039809(SC5D)
TGU: 100226384(SC5D)
LSR: 110477544(SC5D)
SCAN: 103823051(SC5D)
GFR: 102043549(SC5D)
FAB: 101811093(SC5D)
PHI: 102109366(SC5D)
PMAJ: 107214298(SC5D)
CCAE: 111939317(SC5D)
CCW: 104692196(SC5D)
ETL: 114066789(SC5D) 114072326
FPG: 101920444(SC5D)
FCH: 102057522(SC5D)
CLV: 102095349(SC5D)
EGZ: 104125957(SC5D)
NNI: 104016662(SC5D)
ACUN: 113488442(SC5D)
PADL: 103916253(SC5D)
AAM: 106490218(SC5D) 106491711
ASN: 102385911(SC5D)
AMJ: 102571716(SC5D)
PSS: 102463426(SC5D)
CMY: 102931181(SC5D)
CPIC: 101952975(SC5D)
ACS: 100566753(sc5d)
PVT: 110078414(SC5D)
PBI: 103051481(SC5D)
PMUR: 107285543(SC5D)
TSR: 106551450(SC5D)
PMUA: 114585161(SC5D)
GJA: 107125348(SC5D)
XLA: 108697607(sc5d.S) 447026(sc5d.L)
XTR: 100490845(sc5d)
NPR: 108797036(SC5D)
DRE: 447891(sc5d)
SANH: 107703513
IPU: 100528129(sc5d)
PHYP: 113541626(sc5d)
AMEX: 103032395(sc5d)
EEE: 113574240(sc5d)
TRU: 101067117(sc5d)
LCO: 104921101(sc5d) 104938430
ONL: 100695064(sc5d) 100696185
OLA: 101173647(sc5d)
XMA: 102219081(sc5d)
XCO: 114153517(sc5d)
PRET: 103476328(sc5d)
CVG: 107081853(sc5d)
NFU: 107387205(sc5d)
KMR: 108238026(sc5d)
CSEM: 103395378(sc5d)
POV: 109624686 109635651(sc5d)
LCF: 108891924(sc5d) 108901922
SDU: 111225164 111233090(sc5d)
SLAL: 111656091(sc5d) 111666108
HCQ: 109515690(sc5d) 109526775
MALB: 109959324(sc5d)
SASA: 100195087(sc5d) 106576971
OTW: 112253002 112257983(sc5d)
SALP: 111952019 111980773(sc5d)
ELS: 105023255 105024195(sc5d)
SFM: 108936096(sc5d)
PKI: 111844810(sc5d)
LCM: 102365779(SC5D)
CMK: 103190397(sc5d)
RTP: 109938738(sc5d)
SPU: 588561
SKO: 100375039
NVL: 108559476
BMOR: 101737328
BTAB: 109032888
FCD: 110862568
CSCU: 111625209
PCAN: 112567998
MYI: 110461770
PDAM: 113668803
SPIS: 111331638
DGT: 114523409
AQU: 100635449
ATH: AT3G02580(STE1) AT3G02590
CPAP: 110807494
CIT: 102630557
TCC: 18602021
GRA: 105788590
GAB: 108489860
EGR: 104422113
VRA: 106765343
VAR: 108345518
VUN: 114181961
CCAJ: 109791074
CAM: 101511716
LJA: Lj0g3v0103419.1(Lj0g3v0103419.1)
FVE: 101292472
RCN: 112174786
PPER: 18783760
PMUM: 103342673
PAVI: 110763174
CSV: 101204738
CMO: 103487532
MCHA: 111016898
RCU: 8261209
JCU: 105638767
CANN: 107860069
SIND: 105177929
OSA: 4325687
DOSA: Os01t0134500-01(Os01g0134500)
OBR: 102707173
BDI: 100823877
ATS: 109738537(LOC109738537)
SBI: 8061354
SITA: 101780951
DCT: 110108874
PEQ: 110026229
ATR: 18432187
PPP: 112288804
MNG: MNEG_2125
APRO: F751_6009
SCE: YLR056W(ERG3)
ERC: Ecym_1501
KMX: KLMA_20308(ERG3)
NDI: NDAI_0C06430(NDAI0C06430)
TPF: TPHA_0D00440(TPHA0D00440) TPHA_0P00250(TPHA0P00250)
TBL: TBLA_0D02520(TBLA0D02520)
TDL: TDEL_0C00670(TDEL0C00670)
KAF: KAFR_0D01010(KAFR0D01010)
PIC: PICST_28450(ERG3)
SPAA: SPAPADRAFT_48108(ERG3)
CAL: CAALFM_C104770CA(ERG3)
CDU: CD36_04520(ERG3)
SLB: AWJ20_2868(ERG3)
NTE: NEUTE1DRAFT116882(NEUTE1DRAFT_116882) NEUTE1DRAFT78153(NEUTE1DRAFT_78153)
MGR: MGG_08832
SSCK: SPSK_02588
BFU: BCIN_16g04720(Bcerg3)
MBE: MBM_05730
ANG: ANI_1_28134(An15g00150) ANI_1_406144(An16g02930)
PTE: PTT_15444
ZTR: MYCGRDRAFT_74506(ERG3)
SPO: SPAC1687.16c(erg31) SPBC27B12.03c(erg32)
CNE: CNA05010
CNB: CNBA4830
LBC: LACBIDRAFT_190564(ERG3)
ABP: AGABI1DRAFT59529(AGABI1DRAFT_59529)
ABV: AGABI2DRAFT218236(AGABI2DRAFT_218236)
MGL: MGL_0204
MRT: MRET_1111
DFA: DFA_02096
SMIN: v1.2.034753.t1(symbB.v1.2.034753.t1) v1.2.037901.t1(symbB.v1.2.037901.t1)
HCH: HCH_01597
 » show all
Taxonomy
Reference
1  [PMID:14189869]
  Authors
DEMPSEY ME, SEATON JD, SCHROEPFER GJ Jr, TROCKMAN RW.
  Title
THE INTERMEDIARY ROLE OF DELTA-5,7-CHOLESTADIEN-3-BETA-OL IN CHOLESTEROL BIOSYNTHESIS.
  Journal
J Biol Chem 239:1381-7 (1964)
Reference
2  [PMID:4019441]
  Authors
Honjo K, Ishibashi T, Imai Y
  Title
Partial purification and characterization of lathosterol 5-desaturase from rat liver microsomes.
  Journal
J Biochem 97:955-9 (1985)
Reference
3  [PMID:1864507]
  Authors
Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M
  Title
Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.
  Journal
Gene 102:39-44 (1991)
DOI:10.1016/0378-1119(91)90535-J
  Sequence
[sce:YLR056W]
Reference
4  [PMID:8561508]
  Authors
Taton M, Rahier A.
  Title
Plant sterol biosynthesis: identification and characterization of higher plant delta 7-sterol C5(6)-desaturase.
  Journal
Arch Biochem Biophys 325:279-88 (1996)
DOI:10.1006/abbi.1996.0035
Reference
5  [PMID:9056262]
  Authors
Nishino H, Nakaya J, Nishi S, Kurosawa T, Ishibashi T.
  Title
Temperature-induced differential kinetic properties between an initial burst and the following steady state in membrane-bound enzymes: studies on lathosterol 5-desaturase.
  Journal
Arch Biochem Biophys 339:298-304 (1997)
DOI:10.1006/abbi.1996.9871
Reference
6  [PMID:10651635]
  Authors
Taton M, Husselstein T, Benveniste P, Rahier A.
  Title
Role of highly conserved residues in the reaction catalyzed by recombinant Delta7-sterol-C5(6)-desaturase studied by site-directed mutagenesis.
  Journal
Biochemistry 39:701-11 (2000)
DOI:10.1021/bi991467t
  Sequence
[ath:AT3G02580]
Reference
7  [PMID:22982564]
  Authors
Poklepovich TJ, Rinaldi MA, Tomazic ML, Favale NO, Turkewitz AP, Nudel CB, Nusblat AD
  Title
The cytochrome b5 dependent C-5(6) sterol desaturase DES5A from the endoplasmic reticulum of Tetrahymena thermophila complements ergosterol biosynthesis mutants  in Saccharomyces cerevisiae.
  Journal
Steroids 77:1313-20 (2012)
DOI:10.1016/j.steroids.2012.08.015
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.20
IUBMB Enzyme Nomenclature: 1.14.19.20
ExPASy - ENZYME nomenclature database: 1.14.19.20
BRENDA, the Enzyme Database: 1.14.19.20
CAS: 37255-37-1

KEGG   REACTION: R07505Help
Entry
R07505                      Reaction                               

Name
episterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating
Definition
Episterol + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> 5,7,24(28)-Ergostatrienol + 2 Ferricytochrome b5 + 2 H2O
Equation
C15777 + 2 C00999 + C00007 + 2 C00080 <=> C15778 + 2 C00996 + 2 C00001
Reaction class
RC00904  C15777_C15778
Enzyme
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00102  Ergocalciferol biosynthesis
Orthology
K00227  Delta7-sterol 5-desaturase [EC:1.14.19.20]
Other DBs
RHEA: 33466

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