KEGG   ORTHOLOGY: K00317
Entry
K00317                      KO                                     

Name
dmd-tmd
Definition
dimethylamine/trimethylamine dehydrogenase [EC:1.5.8.1 1.5.8.2]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K00317  dmd-tmd; dimethylamine/trimethylamine dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.8  With a flavin or flavoprotein as acceptor
    1.5.8.1  dimethylamine dehydrogenase
     K00317  dmd-tmd; dimethylamine/trimethylamine dehydrogenase
    1.5.8.2  trimethylamine dehydrogenase
     K00317  dmd-tmd; dimethylamine/trimethylamine dehydrogenase
Other DBs
RN: R01588 R02511
COG: COG1902
GO: 0047133 0050470
Genes
PMEX: H4W19_11290
RHD: R2APBS1_0788
DJI: CH75_07125
DTX: ATSB10_15590
XBA: C7S18_14515
PRE: PCA10_47330
PFC: PflA506_4693(tmd)
POI: BOP93_22725
TSN: W908_00015
THIN: CRN91_01375
RSO: RSp0682
RSN: RSPO_m01017(stcD)
RSE: F504_4539
RSY: RSUY_38890(tmd)
BPH: Bphy_5960
PSPU: NA29_08505
CFU: CFU_2684
CPRA: CPter91_1920(tmd)
MEH: M301_1400
MEP: MPQ_2403
SME: SMa0257
SMI: BN406_04939(tmd)
SMER: DU99_24725
SMD: Smed_1285
OAN: Oant_3262
OAH: DR92_3227(hdh)
OCH: CES85_4558(tmd)
XAU: Xaut_0917
BLAG: BLTE_16710
PLEO: OHA_1_00468(tmd_1) OHA_1_00478(tmd_2)
MCG: GL4_2663
RDE: RD1_4141(tmd)
RLI: RLO149_c003340(tmd)
PAMN: pAMV3p0110(dmd) pAMV3p0194(tmd)
OAT: OAN307_c42970(tmd)
OAR: OA238_c47830(tmd1) OA238_c47840(tmd2) OA238_c47850(tmd3)
OTM: OSB_15670(tmd)
RMM: ROSMUCSMR3_03636(tmd)
RID: RIdsm_00662(tmd_1) RIdsm_04243(tmd_2) RIdsm_04244(tmd_3)
ROH: FIU89_18495(tmd)
MALU: KU6B_04850
SMIC: SmB9_20160
MVQ: MYVA_5919
TPR: Tpau_0381
PSIM: KR76_16005
RCA: Rcas_1934
 » show all
Reference
  Authors
Jones M, Talfournier F, Bobrov A, Grossmann JG, Vekshin N, Sutcliffe MJ, Scrutton NS
  Title
Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.
  Journal
J Biol Chem 277:8457-65 (2002)
DOI:10.1074/jbc.M111105200
Reference
PMID:7556160
  Authors
Yang CC, Packman LC, Scrutton NS
  Title
The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition.
  Journal
Eur J Biochem 232:264-71 (1995)
DOI:10.1111/j.1432-1033.1995.tb20808.x
  Sequence
[hdn:Hden_0194]

KEGG   ENZYME: 1.5.8.2
Entry
EC 1.5.8.2                  Enzyme                                 

Name
trimethylamine dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a flavin or flavoprotein as acceptor
Sysname
trimethylamine:electron-transfer flavoprotein oxidoreductase (demethylating)
Reaction(IUBMB)
trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein [RN:R02511]
Reaction(KEGG)
R02511
Substrate
trimethylamine [CPD:C00565];
H2O [CPD:C00001];
electron-transfer flavoprotein [CPD:C04253]
Product
dimethylamine [CPD:C00543];
formaldehyde [CPD:C00067];
reduced electron-transfer flavoprotein [CPD:C04570]
Comment
A number of alkyl-substituted derivatives of trimethylamine can also act as electron donors; phenazine methosulfate and 2,6-dichloroindophenol can act as electron acceptors. Contains FAD and a [4Fe-4S] cluster.
History
EC 1.5.8.2 created 1976 as EC 1.5.99.7, transferred 2002 to EC 1.5.8.2
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00317  dimethylamine/trimethylamine dehydrogenase
Genes
PMEX: H4W19_11290
RHD: R2APBS1_0788
DJI: CH75_07125
DTX: ATSB10_15590
XBA: C7S18_14515
PRE: PCA10_47330
PFC: PflA506_4693(tmd)
POI: BOP93_22725
TSN: W908_00015
THIN: CRN91_01375
RSO: RSp0682
RSN: RSPO_m01017(stcD)
RSE: F504_4539
RSY: RSUY_38890(tmd)
BPH: Bphy_5960
PSPU: NA29_08505
CFU: CFU_2684
CPRA: CPter91_1920(tmd)
MEH: M301_1400
MEP: MPQ_2403
SME: SMa0257
SMI: BN406_04939(tmd)
SMER: DU99_24725
SMD: Smed_1285
OAN: Oant_3262
OAH: DR92_3227(hdh)
OCH: CES85_4558(tmd)
XAU: Xaut_0917
BLAG: BLTE_16710
PLEO: OHA_1_00468(tmd_1) OHA_1_00478(tmd_2)
MCG: GL4_2663
RDE: RD1_4141(tmd)
RLI: RLO149_c003340(tmd)
PAMN: pAMV3p0110(dmd) pAMV3p0194(tmd)
OAT: OAN307_c42970(tmd)
OAR: OA238_c47830(tmd1) OA238_c47840(tmd2) OA238_c47850(tmd3)
OTM: OSB_15670(tmd)
RMM: ROSMUCSMR3_03636(tmd)
RID: RIdsm_00662(tmd_1) RIdsm_04243(tmd_2) RIdsm_04244(tmd_3)
ROH: FIU89_18495(tmd)
MALU: KU6B_04850
SMIC: SmB9_20160
MVQ: MYVA_5919
TPR: Tpau_0381
PSIM: KR76_16005
RCA: Rcas_1934
 » show all
Reference
1  [PMID:5116569]
  Authors
Colby J, Zatman LJ.
  Title
The purification and properties of a bacterial trimethylamine dehydrogenase.
  Journal
Biochem J 121:9P-10P (1971)
DOI:10.1042/bj1210009p
Reference
2  [PMID:204297]
  Authors
Steenkamp DJ, Singer TP.
  Title
Participation of the iron-sulphur cluster and of the covalently bound coenzyme of trimethylamine dehydrogenase in catalysis.
  Journal
Biochem J 169:361-9 (1978)
DOI:10.1042/bj1690361
Reference
3  [PMID:7592591]
  Authors
Huang L, Rohlfs RJ, Hille R.
  Title
The reaction of trimethylamine dehydrogenase with electron transferring flavoprotein.
  Journal
J Biol Chem 270:23958-65 (1995)
DOI:10.1074/jbc.270.41.23958
Reference
4  [PMID:11756429]
  Authors
Jones M, Talfournier F, Bobrov A, Grossmann JG, Vekshin N, Sutcliffe MJ, Scrutton NS
  Title
Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.
  Journal
J Biol Chem 277:8457-65 (2002)
DOI:10.1074/jbc.M111105200
Reference
5  [PMID:11192721]
  Authors
Scrutton NS, Sutcliffe MJ.
  Title
Trimethylamine dehydrogenase and electron transferring flavoprotein.
  Journal
Subcell Biochem 35:145-81 (2000)
DOI:10.1007/0-306-46828-x_5
Other DBs
ExplorEnz - The Enzyme Database: 1.5.8.2
IUBMB Enzyme Nomenclature: 1.5.8.2
ExPASy - ENZYME nomenclature database: 1.5.8.2
UM-BBD (Biocatalysis/Biodegradation Database): 1.5.8.2
BRENDA, the Enzyme Database: 1.5.8.2
CAS: 39307-09-0

KEGG   REACTION: R02511
Entry
R02511                      Reaction                               

Name
trimethylamine:electron-transferring flavoprotein oxidoreductase(demethylating)
Definition
Trimethylamine + H2O + Electron-transferring flavoprotein <=> Dimethylamine + Formaldehyde + Reduced electron-transferring flavoprotein
Equation
Reaction class
RC00185  C00067_C00565
RC00732  C00543_C00565
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Orthology
K00317  dimethylamine/trimethylamine dehydrogenase [EC:1.5.8.1 1.5.8.2]
Other DBs
RHEA: 11867

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