KEGG   ORTHOLOGY: K00320
Entry
K00320                      KO                                     

Name
mer
Definition
5,10-methylenetetrahydromethanopterin reductase [EC:1.5.98.2]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00567  Methanogenesis, CO2 => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.98  With other, known, physiological acceptors
    1.5.98.2  5,10-methylenetetrahydromethanopterin reductase
     K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
Other DBs
RN: R04464
COG: COG2141
GO: 0018537
Genes
ZAL: AZF00_03740
HALC: EY643_08545
KIM: G3T16_19900
PSPW: BJG93_33595
DOE: DENOEST_1262
BIC: LMTR13_24555 LMTR13_24680
RPB: RPB_4412 RPB_4431
NRE: BES08_26800
SWI: Swit_0724
SPHD: HY78_07885 HY78_23075
SPKC: KC8_06245
MPA: MAP_0675
MAO: MAP4_3189
MAVI: RC58_15805
MAVU: RE97_15835
MYO: OEM_23460
MMC: Mmcs_3824
MKM: Mkms_3898
MXE: MYXE_08020(hmd)
MGI: Mflv_0410
MHAS: MHAS_00010(fgd1_1)
MCHT: MCHIJ_11280(hmd_2)
MAIC: MAIC_27490
MMIN: MMIN_35190
MHIB: MHIB_00990(hmd)
GBR: Gbro_3836
GOR: KTR9_3774
GOM: D7316_01986 D7316_02156(fgd2_4)
SGB: WQO_00840
SFI: SFUL_290
SALU: DC74_6554
SALL: SAZ_34125
SLE: sle_06190(sle_06190) sle_10720(sle_10720)
SMAL: SMALA_8438
SGE: DWG14_02663(fgd2_1)
IDO: I598_2061(fgd1_2)
CFL: Cfla_3359
CFI: Celf_0191
NDK: I601_3217
KFL: Kfla_5429
SRO: Sros_4162
MMAR: MODMU_5441
KRA: Krad_3725
AOI: AORI_1327(hmd) AORI_2959
AMQ: AMETH_1112(hmd)
AORI: SD37_34555
PSEE: FRP1_24510
PSEH: XF36_06775
AMI: Amir_2778
SESP: BN6_46520
ACTI: UA75_05060
ACAD: UA74_04945
MIL: ML5_2085
ASE: ACPL_431
ACTN: L083_0261
AFS: AFR_28030
ACTS: ACWT_0316
CWO: Cwoe_5617
ATM: ANT_14450
MJA: MJ_1534
MMP: MMP0058(mer)
MMD: GYY_00295
MMAK: MMKA1_00600(mer)
MMAO: MMOS7_00840(mer)
MMAD: MMJJ_10380
MAE: Maeo_0749
MVO: Mvol_1374
METF: CFE53_02485(mer)
MTH: MTH_1752
MMG: MTBMA_c03270(mer)
METC: MTCT_1602
MWO: MWSIV6_0292(mer)
METE: tca_01698
METK: FVF72_02910(mer)
MTHM: FZP57_01345(mer)
MST: Msp_1128(mer)
MRU: mru_0569(mer)
MSI: Msm_0542
MEB: Abm4_1417(mer)
MMIL: sm9_0419(mer)
MEYE: TL18_02180
MOL: YLM1_0233
METH: MBMB1_1520(mer)
MFC: BRM9_0745(mer)
MFI: DSM1535_0704(mer)
MCUB: MCBB_1769(mer)
METT: CIT01_07510(mer)
METO: CIT02_10410(mer)
MFV: Mfer_0274
MKA: MK0524(mer)
AFU: AF_1066
FPL: Ferp_0743
GAC: GACE_0389
GAH: GAH_01105
MBAR: MSBR2_2654
MAC: MA_3733(mer)
MMA: MM_0628
MMAC: MSMAC_0682
METM: MSMTP_0746
MTHR: MSTHT_2388
MTHE: MSTHC_0892
MHOR: MSHOH_0799
MFZ: AOB57_003480(mer)
MBU: Mbur_2372(mer)
MMET: MCMEM_0517
MMH: Mmah_1513
MPY: Mpsy_2035
MZI: HWN40_11975(mer)
MTP: Mthe_0205
MCJ: MCON_2394(mer)
MHI: Mhar_0856
MHU: Mhun_2257
MLA: Mlab_0076
MBG: BN140_1674(mer)
MEMA: MMAB1_2155(mer)
MPI: Mpet_2180
MBN: Mboo_0528
MPL: Mpal_0327
MPD: MCP_2681(mer)
MEZ: Mtc_0135(mer)
RCI: RCIX564(mer)
HHB: Hhub_1106(mer)
HSU: HLASF_0416(mer)
HSF: HLASA_0415(mer)
HMA: rrnAC2437(mer1)
HHI: HAH_2870(mer3)
NPH: NP_1056A(mer)
NMO: Nmlp_1133(mer)
HUT: Huta_1395
HTI: HTIA_1288
HMU: Hmuk_0341
HALL: LC1Hm_2870(mer)
HWA: HQ_3154A(mer)
HWC: Hqrw_3697(mer)
HVO: HVO_1937(mer)
HME: HFX_2030(mer)
HLA: Hlac_1680
HDF: AArcSl_2514(mer)
HTU: Htur_3573
NMG: Nmag_1582(mer)
NAT: NJ7G_2905
SALI: L593_00135
NVN: NVIE_004930(mer)
NEV: NTE_01814
NCV: NCAV_1415
CCAI: NAS2_0789
BARC: AOA65_0782(mer_2)
 » show all
Reference
PMID:2298726
  Authors
te Brommelstroet BW, Hensgens CM, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.
  Journal
J Biol Chem 265:1852-7 (1990)
  Sequence
[mth:MTH_1752]
Reference
PMID:2379499
  Authors
Ma K, Thauer RK
  Title
Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur J Biochem 191:187-93 (1990)
DOI:10.1111/j.1432-1033.1990.tb19109.x
  Sequence

KEGG   ENZYME: 1.5.98.2
Entry
EC 1.5.98.2                 Enzyme                                 

Name
5,10-methylenetetrahydromethanopterin reductase;
5,10-methylenetetrahydromethanopterin cyclohydrolase;
N5,N10-methylenetetrahydromethanopterin reductase;
methylene-H4MPT reductase;
coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase;
N5,N10-methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With other, known, physiological acceptors
Sysname
5-methyltetrahydromethanopterin:coenzyme-F420 oxidoreductase
Reaction(IUBMB)
5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420 [RN:R04464]
Reaction(KEGG)
R04464
Substrate
5-methyltetrahydromethanopterin [CPD:C04488];
oxidized coenzyme F420 [CPD:C00876]
Product
5,10-methylenetetrahydromethanopterin [CPD:C04377];
reduced coenzyme F420 [CPD:C01080]
Comment
Catalyses an intermediate step in methanogenesis from CO2 and H2 in methanogenic archaea.
History
EC 1.5.98.2 created 2000 as EC 1.5.99.11, modified 2004, transferred to EC 1.5.98.2 2014
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00320  5,10-methylenetetrahydromethanopterin reductase
Genes
ZAL: AZF00_03740
HALC: EY643_08545
KIM: G3T16_19900
PSPW: BJG93_33595
DOE: DENOEST_1262
BIC: LMTR13_24555 LMTR13_24680
RPB: RPB_4412 RPB_4431
NRE: BES08_26800
SWI: Swit_0724
SPHD: HY78_07885 HY78_23075
SPKC: KC8_06245
MPA: MAP_0675
MAO: MAP4_3189
MAVI: RC58_15805
MAVU: RE97_15835
MYO: OEM_23460
MMC: Mmcs_3824
MKM: Mkms_3898
MXE: MYXE_08020(hmd)
MGI: Mflv_0410
MHAS: MHAS_00010(fgd1_1)
MCHT: MCHIJ_11280(hmd_2)
MAIC: MAIC_27490
MMIN: MMIN_35190
MHIB: MHIB_00990(hmd)
GBR: Gbro_3836
GOR: KTR9_3774
GOM: D7316_01986 D7316_02156(fgd2_4)
SGB: WQO_00840
SFI: SFUL_290
SALU: DC74_6554
SALL: SAZ_34125
SLE: sle_06190(sle_06190) sle_10720(sle_10720)
SMAL: SMALA_8438
SGE: DWG14_02663(fgd2_1)
IDO: I598_2061(fgd1_2)
CFL: Cfla_3359
CFI: Celf_0191
NDK: I601_3217
KFL: Kfla_5429
SRO: Sros_4162
MMAR: MODMU_5441
KRA: Krad_3725
AOI: AORI_1327(hmd) AORI_2959
AMQ: AMETH_1112(hmd)
AORI: SD37_34555
PSEE: FRP1_24510
PSEH: XF36_06775
AMI: Amir_2778
SESP: BN6_46520
ACTI: UA75_05060
ACAD: UA74_04945
MIL: ML5_2085
ASE: ACPL_431
ACTN: L083_0261
AFS: AFR_28030
ACTS: ACWT_0316
CWO: Cwoe_5617
ATM: ANT_14450
MJA: MJ_1534
MMP: MMP0058(mer)
MMD: GYY_00295
MMAK: MMKA1_00600(mer)
MMAO: MMOS7_00840(mer)
MMAD: MMJJ_10380
MAE: Maeo_0749
MVO: Mvol_1374
METF: CFE53_02485(mer)
MTH: MTH_1752
MMG: MTBMA_c03270(mer)
METC: MTCT_1602
MWO: MWSIV6_0292(mer)
METE: tca_01698
METK: FVF72_02910(mer)
MTHM: FZP57_01345(mer)
MST: Msp_1128(mer)
MRU: mru_0569(mer)
MSI: Msm_0542
MEB: Abm4_1417(mer)
MMIL: sm9_0419(mer)
MEYE: TL18_02180
MOL: YLM1_0233
METH: MBMB1_1520(mer)
MFC: BRM9_0745(mer)
MFI: DSM1535_0704(mer)
MCUB: MCBB_1769(mer)
METT: CIT01_07510(mer)
METO: CIT02_10410(mer)
MFV: Mfer_0274
MKA: MK0524(mer)
AFU: AF_1066
FPL: Ferp_0743
GAC: GACE_0389
GAH: GAH_01105
MBAR: MSBR2_2654
MAC: MA_3733(mer)
MMA: MM_0628
MMAC: MSMAC_0682
METM: MSMTP_0746
MTHR: MSTHT_2388
MTHE: MSTHC_0892
MHOR: MSHOH_0799
MFZ: AOB57_003480(mer)
MBU: Mbur_2372(mer)
MMET: MCMEM_0517
MMH: Mmah_1513
MPY: Mpsy_2035
MZI: HWN40_11975(mer)
MTP: Mthe_0205
MCJ: MCON_2394(mer)
MHI: Mhar_0856
MHU: Mhun_2257
MLA: Mlab_0076
MBG: BN140_1674(mer)
MEMA: MMAB1_2155(mer)
MPI: Mpet_2180
MBN: Mboo_0528
MPL: Mpal_0327
MPD: MCP_2681(mer)
MEZ: Mtc_0135(mer)
RCI: RCIX564(mer)
HHB: Hhub_1106(mer)
HSU: HLASF_0416(mer)
HSF: HLASA_0415(mer)
HMA: rrnAC2437(mer1)
HHI: HAH_2870(mer3)
NPH: NP_1056A(mer)
NMO: Nmlp_1133(mer)
HUT: Huta_1395
HTI: HTIA_1288
HMU: Hmuk_0341
HALL: LC1Hm_2870(mer)
HWA: HQ_3154A(mer)
HWC: Hqrw_3697(mer)
HVO: HVO_1937(mer)
HME: HFX_2030(mer)
HLA: Hlac_1680
HDF: AArcSl_2514(mer)
HTU: Htur_3573
NMG: Nmag_1582(mer)
NAT: NJ7G_2905
SALI: L593_00135
NVN: NVIE_004930(mer)
NEV: NTE_01814
NCV: NCAV_1415
CCAI: NAS2_0789
BARC: AOA65_0782(mer_2)
 » show all
Reference
1  [PMID:2379499]
  Authors
Ma K, Thauer RK
  Title
Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur J Biochem 191:187-93 (1990)
DOI:10.1111/j.1432-1033.1990.tb19109.x
  Sequence
Reference
2  [PMID:1911853]
  Authors
te Brommelstroet BW, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri.
  Journal
Biochim Biophys Acta 1079:293-302 (1991)
DOI:10.1016/0167-4838(91)90072-8
Reference
3  [PMID:1696553]
  Authors
Ma K, Thauer RK.
  Title
Single step purification of methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum by specific binding to blue sepharose CL-6B.
  Journal
FEBS Lett 268:59-62 (1990)
DOI:10.1016/0014-5793(90)80972-L
Reference
4  [PMID:2298726]
  Authors
te Brommelstroet BW, Hensgens CM, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.
  Journal
J Biol Chem 265:1852-7 (1990)
  Sequence
[mth:MTH_1752]
Reference
5  [PMID:2298699]
  Authors
te Brommelstroet BW, Hensgens CM, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD.
  Title
Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri.
  Journal
J Bacteriol 172:564-71 (1990)
DOI:10.1128/JB.172.2.564-571.1990
Other DBs
ExplorEnz - The Enzyme Database: 1.5.98.2
IUBMB Enzyme Nomenclature: 1.5.98.2
ExPASy - ENZYME nomenclature database: 1.5.98.2
BRENDA, the Enzyme Database: 1.5.98.2

KEGG   REACTION: R04464
Entry
R04464                      Reaction                               

Name
5,10-Methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase
Definition
5,10-Methylenetetrahydromethanopterin + Reduced coenzyme F420 <=> 5-Methyl-5,6,7,8-tetrahydromethanopterin + Coenzyme F420
Equation
Reaction class
RC01607  C04377_C04488
RC02628  C00876_C01080
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00567  Methanogenesis, CO2 => methane
Orthology
K00320  5,10-methylenetetrahydromethanopterin reductase [EC:1.5.98.2]
Other DBs
RHEA: 21147

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