| Entry |
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| Name |
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT1 (gene name);
N-acetylglucosaminyltransferase I;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase;
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I;
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I;
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTI;
GlcNAc-T I;
UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
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| Class |
Transferases;
Glycosyltransferases;
Hexosyltransferases
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| Sysname |
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
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| Reaction(IUBMB) |
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] [RN: R05983]
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| Reaction(KEGG) |
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| Substrate |
UDP-N-acetyl-alpha-D-glucosamine [CPD: C00043];
Man5GlcNAc2-[protein]
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| Product |
UDP [CPD: C00015];
Man5GlcNAc3-[protein]
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| Comment |
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its action is required before the other N-acetylglucosaminyltransferases involved in the process (GlcNAcT-II through VI) can act. While the natural substrate (produced by EC 3.2.1.113, mannosyl-oligosaccharide 1,2-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->3)-beta-D-Man-R.
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| History |
EC 2.4.1.101 created 1983, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
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| Pathway |
| ec00513 | Various types of N-glycan biosynthesis |
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| Orthology |
| K00726 | alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase |
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| Genes |
» show all
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| Reference |
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| Authors |
Harpaz N, Schachter H. |
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| Title |
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity. |
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| Journal |
J Biol Chem 255:4885-93 (1980) |
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| Reference |
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| Authors |
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M. |
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| Title |
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa. |
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| Journal |
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| Reference |
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| Authors |
Oppenheimer CL, Hill RL. |
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| Title |
Purification and characterization of a rabbit liver alpha 1 goes to 3 mannoside beta 1 goes to 2 N-acetylglucosaminyltransferase. |
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| Journal |
J Biol Chem 256:799-804 (1981) |
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| Reference |
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| Authors |
Oppenheimer CL, Eckhardt AE, Hill RL. |
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| Title |
The nonidentity of porcine N-acetylglucosaminyltransferases I and II. |
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| Journal |
J Biol Chem 256:11477-82 (1981) |
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| Reference |
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| Authors |
Miyagi T, Tsuiki S. |
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| Title |
Studies on UDP-N-acetylglucosamine : alpha-mannoside beta-N-acetylglucosaminyltransferase of rat liver and hepatomas. |
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| Journal |
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| Reference |
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| Authors |
Schachter H, Narasimhan S, Gleeson P, Vella G. |
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| Title |
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type. |
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| Journal |
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| Reference |
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| Authors |
Vella GJ, Paulsen H, Schachter H. |
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| Title |
Control of glycoprotein synthesis. IX. A terminal Man alpha l-3Man beta 1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: alpha-D-mannoside (GlcNAc to Man alpha 1-3) beta 2-N-acetylglucosaminyltransferase I. |
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| Journal |
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| Reference |
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| Authors |
Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM. |
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| Title |
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.4.1.101 |
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