KEGG   ORTHOLOGY: K00727
Entry
K00727                      KO                                     

Name
GCNT1
Definition
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase [EC:2.4.1.102]
Pathway
ko00512  Mucin type O-glycan biosynthesis
ko01100  Metabolic pathways
Module
M00056  O-glycan biosynthesis, mucin type core
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09107 Glycan biosynthesis and metabolism
   00512 Mucin type O-glycan biosynthesis
    K00727  GCNT1; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01003 Glycosyltransferases
    K00727  GCNT1; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.102  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
     K00727  GCNT1; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Glycosyltransferases [BR:ko01003]
 Glycan extension
  O-Glycan
   K00727  GCNT1; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Other DBs
RN: R05910 R05912
GO: 0003829
CAZy: GT14
Genes
HSA: 2650(GCNT1)
PTR: 472959(GCNT1)
PPS: 100990909(GCNT1)
GGO: 101144738(GCNT1)
PON: 100453351(GCNT1)
NLE: 100590764(GCNT1)
MCC: 704639(GCNT1)
MCF: 102121951(GCNT1)
CSAB: 103219612(GCNT1)
RRO: 104682177(GCNT1)
RBB: 108542654(GCNT1)
CJC: 100397450(GCNT1)
SBQ: 101041046(GCNT1)
MMU: 14537(Gcnt1)
MCAL: 110285874(Gcnt1)
MPAH: 110334399(Gcnt1)
RNO: 64043(Gcnt1)
MUN: 110557756(Gcnt1)
CGE: 100767124(Gcnt1)
NGI: 103730640
HGL: 101713056(Gcnt1)
CCAN: 109693363(Gcnt1)
OCU: 100347030(GCNT1)
TUP: 102474618(GCNT1)
CFA: 484158(GCNT1)
VVP: 112922094(GCNT1)
AML: 100481771(GCNT1)
UMR: 103663275(GCNT1)
UAH: 113269836(GCNT1)
ORO: 101386761(GCNT1)
ELK: 111153608
FCA: 101093969(GCNT1)
PTG: 102952027(GCNT1)
PPAD: 109275372(GCNT1)
AJU: 106975313(GCNT1)
BTA: 281778(GCNT1)
BOM: 102285997(GCNT1)
BIU: 109562970(GCNT1)
BBUB: 102389154(GCNT1)
CHX: 102187590(GCNT1)
OAS: 101121316(GCNT1)
SSC: 106508649(GCNT1)
CFR: 102509033(GCNT1)
CDK: 105087407(GCNT1)
BACU: 103015488(GCNT1)
LVE: 103084201(GCNT1)
OOR: 101290151(GCNT1)
DLE: 111174579(GCNT1)
PCAD: 102994842(GCNT1)
ECB: 100061416(GCNT1)
EPZ: 103548068(GCNT1)
EAI: 106825720(GCNT1)
MYB: 102248045(GCNT1)
MYD: 102759783(GCNT1)
MNA: 107544975(GCNT1)
HAI: 109385744(GCNT1)
DRO: 112300312(GCNT1)
PALE: 102898105(GCNT1)
RAY: 107517197(GCNT1)
MJV: 108405009(GCNT1)
LAV: 100658096(GCNT1)
TMU: 101353425
MDO: 100011966(GCNT1)
SHR: 100921847(GCNT1)
PCW: 110214553(GCNT1)
OAA: 100075656(GCNT1)
GGA: 427260(GCNT1)
MGP: 100544057(GCNT1)
CJO: 107306219(GCNT1)
NMEL: 110390082(GCNT1)
APLA: 101796875(GCNT1)
ACYG: 106044482(GCNT1)
TGU: 100221628(GCNT1)
LSR: 110470687(GCNT1)
SCAN: 103819436(GCNT1)
GFR: 102041616(GCNT1)
FAB: 101808578(GCNT1)
PHI: 102103854(GCNT1)
PMAJ: 107198206(GCNT1)
CCAE: 111940833(GCNT1)
CCW: 104691986(GCNT1)
ETL: 114072064(GCNT1)
FPG: 101912116(GCNT1)
FCH: 102047162(GCNT1)
CLV: 102085044(GCNT1)
EGZ: 104123222(GCNT1)
NNI: 104020877(GCNT1)
ACUN: 113490172(GCNT1)
PADL: 103920382(GCNT1)
AAM: 106489914
ASN: 102385228(GCNT1)
AMJ: 102563225(GCNT1)
PSS: 102449351
CMY: 102941735(GCNT1)
CPIC: 101952241(GCNT1)
ACS: 100565751(gcnt1)
PVT: 110076011(GCNT1)
PBI: 103048092(GCNT1)
PMUR: 107286167(GCNT1)
TSR: 106544714(GCNT1)
PMUA: 114606431(GCNT1)
GJA: 107108744(GCNT1)
XLA: 380501(gcnt1.L) 495090
XTR: 100490337(gcnt1)
NPR: 108788489(GCNT1)
IPU: 108265617(gcnt1)
PHYP: 113533424
AMEX: 103024919(gcnt1) 111189883
EEE: 113567652(gcnt1)
TRU: 101066002
TNG: GSTEN00029414G001
LCO: 104920349 104920350
MZE: 101481222 101481511 101485293 112435648
ONL: 100695077 100695236 100695500 100711034 102079779 109194425 109201782 109204432
OLA: 101157299(gcnt1)
XMA: 102217069
PRET: 103470242(gcnt1)
CVG: 107087256
NFU: 107394005 107394006 107394008
KMR: 108234497 108234507
ALIM: 106523800 106523801
AOCE: 111575565 111576639
POV: 109638064(gcnt1)
LCF: 108878093(gcnt1)
SDU: 111230816 111230818
SLAL: 111652911(gcnt1)
HCQ: 109520688(gcnt1)
SASA: 106585626(gcnt1)
OTW: 112249449 112249463
SFM: 108938500(gcnt1)
PKI: 111839312(gcnt1)
LCM: 102357208(GCNT1)
CMK: 103186097(gcnt1) 103188128
RTP: 109928202(gcnt1)
APLC: 110984114
SKO: 100372443 100372744 100373957 100375210 100379011
CEL: CELE_C54C8.11(gly-15) CELE_T15D6.2(gly-16)
CBR: CBG04363(Cbr-gly-15)
NAI: NECAME_01847 NECAME_01861 NECAME_07494
HRO: HELRODRAFT_106314 HELRODRAFT_141556 HELRODRAFT_72329
LGI: LOTGIDRAFT_104647 LOTGIDRAFT_108047 LOTGIDRAFT_163866 LOTGIDRAFT_54803 LOTGIDRAFT_73452
PCAN: 112553289 112553290 112554440 112561191 112562773 112562774 112562775 112568345 112568760 112568761 112568762 112568892 112570429 112571042 112574428 112575127
CRG: 105334548 105336560 105336571 105336746 105336756
MYI: 110445188 110449075 110455573 110456784 110458069 110465295
OBI: 106873990 106881820 106881829 106883007
LAK: 106150782 106155088 106155707 106155709 106174181 106181126
SMM: Smp_009810 Smp_154680
SHX: MS3_06656 MS3_06659
EGL: EGR_09263
DGT: 114537562 114537592
TAD: TRIADDRAFT_51949
VG: 15486285(EE46) 20098546(EE46) 26196520(E4)
 » show all
Reference
PMID:1329093
  Authors
Bierhuizen MF, Fukuda M
  Title
Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.
  Journal
Proc Natl Acad Sci U S A 89:9326-330 (1992)
DOI:10.1073/pnas.89.19.9326
  Sequence
[hsa:2650]

  All links  
Ontology (5)   
   KEGG BRITE (3)   
   GO (1)   
   CAZY (1)   
Pathway (5)   
   KEGG PATHWAY (4)   
   KEGG MODULE (1)   
Chemical reaction (3)   
   KEGG ENZYME (1)   
   KEGG REACTION (2)   
Gene (562)   
   KEGG GENES (223)   
   KEGG MGENES (54)   
   RefGene (282)   
   OC (3)   
Literature (1)   
   PubMed (1)   
All databases (576)   

Download RDF
KEGG   ENZYME: 2.4.1.102
Entry
EC 2.4.1.102                Enzyme                                 

Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I;
beta6-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-mucin beta-(1->6)-acetylglucosaminyltransferase;
core 2 acetylglucosaminyltransferase;
core 6-beta-GlcNAc-transferase A;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to N-acetyl-D-galactosamine of beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R) beta-1,6-N-acetyl-D-glucosaminyltransferase;
GCNT1;
GCNT3;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to N-acetyl-D-galactosamine of beta-D-galactosyl-(1->3)-N-acetyl-D-galactosaminyl-R) 6-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-alpha-D-glucosamine:O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl/threonyl-[protein] = UDP + O3-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04575 R05912]
Reaction(KEGG)
R04575 R05912(G);
(other) R05910(G)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl/threonyl-[protein]
Product
UDP [CPD:C00015];
O3-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Comment
The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 1 structure of O-glycans forming core 2.
History
EC 2.4.1.102 created 1983, modified 2018
Pathway
ec00512  Mucin type O-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00727  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Genes
HSA: 2650(GCNT1)
PTR: 472959(GCNT1)
PPS: 100990909(GCNT1)
GGO: 101144738(GCNT1)
PON: 100453351(GCNT1)
NLE: 100590764(GCNT1)
MCC: 704639(GCNT1)
MCF: 102121951(GCNT1)
CSAB: 103219612(GCNT1)
RRO: 104682177(GCNT1)
RBB: 108542654(GCNT1)
CJC: 100397450(GCNT1)
SBQ: 101041046(GCNT1)
MMU: 14537(Gcnt1)
MCAL: 110285874(Gcnt1)
MPAH: 110334399(Gcnt1)
RNO: 64043(Gcnt1)
MUN: 110557756(Gcnt1)
CGE: 100767124(Gcnt1)
NGI: 103730640
HGL: 101713056(Gcnt1)
CCAN: 109693363(Gcnt1)
OCU: 100347030(GCNT1)
TUP: 102474618(GCNT1)
CFA: 484158(GCNT1)
VVP: 112922094(GCNT1)
AML: 100481771(GCNT1)
UMR: 103663275(GCNT1)
UAH: 113269836(GCNT1)
ORO: 101386761(GCNT1)
ELK: 111153608
FCA: 101093969(GCNT1)
PTG: 102952027(GCNT1)
PPAD: 109275372(GCNT1)
AJU: 106975313(GCNT1)
BTA: 281778(GCNT1)
BOM: 102285997(GCNT1)
BIU: 109562970(GCNT1)
BBUB: 102389154(GCNT1)
CHX: 102187590(GCNT1)
OAS: 101121316(GCNT1)
SSC: 106508649(GCNT1)
CFR: 102509033(GCNT1)
CDK: 105087407(GCNT1)
BACU: 103015488(GCNT1)
LVE: 103084201(GCNT1)
OOR: 101290151(GCNT1)
DLE: 111174579(GCNT1)
PCAD: 102994842(GCNT1)
ECB: 100061416(GCNT1)
EPZ: 103548068(GCNT1)
EAI: 106825720(GCNT1)
MYB: 102248045(GCNT1)
MYD: 102759783(GCNT1)
MNA: 107544975(GCNT1)
HAI: 109385744(GCNT1)
DRO: 112300312(GCNT1)
PALE: 102898105(GCNT1)
RAY: 107517197(GCNT1)
MJV: 108405009(GCNT1)
LAV: 100658096(GCNT1)
TMU: 101353425
MDO: 100011966(GCNT1)
SHR: 100921847(GCNT1)
PCW: 110214553(GCNT1)
OAA: 100075656(GCNT1)
GGA: 427260(GCNT1)
MGP: 100544057(GCNT1)
CJO: 107306219(GCNT1)
NMEL: 110390082(GCNT1)
APLA: 101796875(GCNT1)
ACYG: 106044482(GCNT1)
TGU: 100221628(GCNT1)
LSR: 110470687(GCNT1)
SCAN: 103819436(GCNT1)
GFR: 102041616(GCNT1)
FAB: 101808578(GCNT1)
PHI: 102103854(GCNT1)
PMAJ: 107198206(GCNT1)
CCAE: 111940833(GCNT1)
CCW: 104691986(GCNT1)
ETL: 114072064(GCNT1)
FPG: 101912116(GCNT1)
FCH: 102047162(GCNT1)
CLV: 102085044(GCNT1)
EGZ: 104123222(GCNT1)
NNI: 104020877(GCNT1)
ACUN: 113490172(GCNT1)
PADL: 103920382(GCNT1)
AAM: 106489914
ASN: 102385228(GCNT1)
AMJ: 102563225(GCNT1)
PSS: 102449351
CMY: 102941735(GCNT1)
CPIC: 101952241(GCNT1)
ACS: 100565751(gcnt1)
PVT: 110076011(GCNT1)
PBI: 103048092(GCNT1)
PMUR: 107286167(GCNT1)
TSR: 106544714(GCNT1)
PMUA: 114606431(GCNT1)
GJA: 107108744(GCNT1)
XLA: 380501(gcnt1.L) 495090
XTR: 100490337(gcnt1)
NPR: 108788489(GCNT1)
IPU: 108265617(gcnt1)
PHYP: 113533424
AMEX: 103024919(gcnt1) 111189883
EEE: 113567652(gcnt1)
TRU: 101066002
TNG: GSTEN00029414G001
LCO: 104920349 104920350
MZE: 101481222 101481511 101485293 112435648
ONL: 100695077 100695236 100695500 100711034 102079779 109194425 109201782 109204432
OLA: 101157299(gcnt1)
XMA: 102217069
PRET: 103470242(gcnt1)
CVG: 107087256
NFU: 107394005 107394006 107394008
KMR: 108234497 108234507
ALIM: 106523800 106523801
AOCE: 111575565 111576639
POV: 109638064(gcnt1)
LCF: 108878093(gcnt1)
SDU: 111230816 111230818
SLAL: 111652911(gcnt1)
HCQ: 109520688(gcnt1)
SASA: 106585626(gcnt1)
OTW: 112249449 112249463
SFM: 108938500(gcnt1)
PKI: 111839312(gcnt1)
LCM: 102357208(GCNT1)
CMK: 103186097(gcnt1) 103188128
RTP: 109928202(gcnt1)
APLC: 110984114
SKO: 100372443 100372744 100373957 100375210 100379011
CEL: CELE_C54C8.11(gly-15) CELE_T15D6.2(gly-16)
CBR: CBG04363(Cbr-gly-15)
NAI: NECAME_01847 NECAME_01861 NECAME_07494
HRO: HELRODRAFT_106314 HELRODRAFT_141556 HELRODRAFT_72329
LGI: LOTGIDRAFT_104647 LOTGIDRAFT_108047 LOTGIDRAFT_163866 LOTGIDRAFT_54803 LOTGIDRAFT_73452
PCAN: 112553289 112553290 112554440 112561191 112562773 112562774 112562775 112568345 112568760 112568761 112568762 112568892 112570429 112571042 112574428 112575127
CRG: 105334548 105336560 105336571 105336746 105336756
MYI: 110445188 110449075 110455573 110456784 110458069 110465295
OBI: 106873990 106881820 106881829 106883007
LAK: 106150782 106155088 106155707 106155709 106174181 106181126
SMM: Smp_009810 Smp_154680
SHX: MS3_06656 MS3_06659
EGL: EGR_09263
DGT: 114537562 114537592
TAD: TRIADDRAFT_51949
 » show all
Reference
1  [PMID:6226356]
  Authors
Brockhausen I, Rachaman ES, Matta KL, Schachter H.
  Title
The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.
  Journal
Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1
Reference
2  [PMID:6449508]
  Authors
Williams D, Longmore G, Matta KL, Schachter H.
  Title
Mucin synthesis. II. Substrate specificity and product identification studies on canine submaxillary gland UDP-GlcNAc:Gal beta 1-3GalNAc(GlcNAc leads to GalNAc) beta 6-N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 255:11253-61 (1980)
Reference
3  [PMID:6449507]
  Authors
Williams D, Schachter H.
  Title
Mucin synthesis. I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates.
  Journal
J Biol Chem 255:11247-52 (1980)
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.102
IUBMB Enzyme Nomenclature: 2.4.1.102
ExPASy - ENZYME nomenclature database: 2.4.1.102
BRENDA, the Enzyme Database: 2.4.1.102
CAS: 95978-15-7

  All links  
Pathway (5)   
   KEGG PATHWAY (4)   
   KEGG MODULE (1)   
Chemical substance (10)   
   KEGG COMPOUND (4)   
   KEGG GLYCAN (6)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (560)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (223)   
   KEGG MGENES (54)   
   RefGene (282)   
Protein sequence (105)   
   UniProt (43)   
   SWISS-PROT (19)   
   RefSeq(pep) (32)   
   PDBSTR (10)   
   PMD (1)   
DNA sequence (84)   
   RefSeq(nuc) (35)   
   GenBank (29)   
   EMBL (20)   
3D Structure (3)   
   PDB (3)   
Protein domain (1)   
   InterPro (1)   
All databases (773)   

Download RDF
KEGG   REACTION: R05910
Entry
R05910                      Reaction                               

Definition
UDP-N-acetyl-D-glucosamine + Tn antigen <=> UDP + G00031
Equation
Enzyme
2.4.1.102       2.4.1.148
Pathway
rn00512  Mucin type O-glycan biosynthesis
rn01100  Metabolic pathways
Module
M00056  O-glycan biosynthesis, mucin type core
Orthology
K00727  beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase [EC:2.4.1.102]

  All links  
Ontology (2)   
   KEGG BRITE (2)   
Pathway (5)   
   KEGG PATHWAY (4)   
   KEGG MODULE (1)   
Chemical substance (4)   
   KEGG GLYCAN (4)   
Chemical reaction (2)   
   KEGG ENZYME (2)   
Gene (224)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (223)   
All databases (237)   

Download RDF
DBGET integrated database retrieval system