KEGG   ORTHOLOGY: K00808
Entry
K00808                      KO                                     

Name
hss
Definition
homospermidine synthase [EC:2.5.1.44]
Pathway
ko00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00960 Tropane, piperidine and pyridine alkaloid biosynthesis
    K00808  hss; homospermidine synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.44  homospermidine synthase
     K00808  hss; homospermidine synthase
Other DBs
RN: R00018
GO: 0047296
Genes
PAU: PA14_35790
PAP: PSPA7_3061
PDK: PADK2_14350
PAES: SCV20265_3124
PAEU: BN889_06565
PST: PSPTO_1868(hss)
PSK: U771_27265
PCZ: PCL1606_20560
PSEM: TO66_21620
PANR: A7J50_5695
SAZ: Sama_3337
SKH: STH12_03268(hss)
AMAL: I607_20267
AMAE: I876_01355
AMAO: I634_01455
AMAI: I635_21559
ASIP: AQUSIP_02550(hss_1) AQUSIP_19380(hss_2)
LPN: lpg2495(hss)
LPH: LPV_2823
LPO: LPO_2687
LPM: LP6_2525(hss)
LPF: lpl2416
LPP: lpp2562
LPC: LPC_1979(hss)
LPA: lpa_03638
LPE: lp12_2487
LLO: LLO_3342(hss)
LHA: LHA_0258(hss) LHA_0415(hss)
LOK: Loa_01233
LCD: clem_13100(hss_1) clem_13880(hss_3)
LLG: 44548918_00308(hss)
LJR: NCTC11533_00329(hss)
LCJ: NCTC11976_00392(hss)
TMC: LMI_1387(hss)
REH: H16_A0043(hss)
CGD: CR3_2929(hss)
CBX: Cenrod_1499(hss)
OFO: BRW83_1089(hss)
BBAG: E1O_25610
PBH: AAW51_2240(hss)
NEU: NE1498(hss)
MLO: mlr3014
MHUA: MCHK_4530
MES: Meso_2727
AMIH: CO731_02153(hss)
PLA: Plav_1354
SME: SMc04016(hss)
SMX: SM11_chr2965(hss)
SMI: BN406_02658(hss)
SMEL: SM2011_c04016(hss)
SMER: DU99_15480
SMD: Smed_2688
SFD: USDA257_c52690(hss)
SAME: SAMCFNEI73_Ch3223(hss)
EAD: OV14_0297
ATU: Atu3768
ARA: Arad_3814(hss)
AVI: Avi_3707
RET: RHE_CH03553(hss)
REL: REMIM1_CH03624(hss)
REP: IE4803_CH03928(hss)
REI: IE4771_CH03866(hss)
RLE: RL4073(hss)
RLG: Rleg_3602
RIR: BN877_II0721(hss)
RHL: LPU83_3541(hss)
RGA: RGR602_CH03450(hss)
RHN: AMJ98_CH03777(hss)
RPHA: AMC79_CH03729(hss)
RHT: NT26_2725(hss)
RHX: AMK02_CH03666(hss)
RHK: Kim5_CH03896(hss)
REZ: AMJ99_CH03830(hss)
NEN: NCHU2750_27630(hss)
SHZ: shn_15290
BMEL: DK63_2103(hss)
BMEE: DK62_2173(hss)
BMF: BAB2_0077
BABO: DK55_3182(hss)
BABR: DO74_2368(hss)
BABT: DK49_2186(hss)
BABB: DK48_2615(hss)
BABU: DK53_3056(hss)
BABS: DK51_2540(hss)
BABC: DO78_3101(hss)
BMS: BRA0078
BSZ: DK67_2110(hss)
BOV: BOV_A0073
BCS: BCAN_B0081(hss)
BCAR: DK60_2965(hss)
BCAS: DA85_10890
BMR: BMI_II80
BPP: BPI_II80
BPV: DK65_2381(hss)
OAN: Oant_4263
OAH: DR92_3986(hss)
OCH: CES85_5524(hss)
BJA: blr7762
BRA: BRADO6264(hss)
BBT: BBta_1345(hss)
BRS: S23_63280
AOL: S58_64330
BRAD: BF49_3189
BRO: BRAD285_6208(hss)
RPA: RPA0865
RPB: RPB_4552
RPC: RPC_4834
RPD: RPD_0850
RPE: RPE_4799
RPT: Rpal_0933
NWI: Nwi_0921
NHA: Nham_3423
OCA: OCAR_4912
OCG: OCA5_c30420(hss)
OCO: OCA4_c29900(hss)
VGO: GJW-30_1_02467(hss)
XAU: Xaut_2138
AZC: AZC_0296
SNO: Snov_3872
MEA: Mex_1p2024(hss)
MDI: METDI2807(hss) METDI4480(hss)
MEX: Mext_2042
MPO: Mpop_2003
MET: M446_0451
MNO: Mnod_1646
MOR: MOC_5748
META: Y590_09740
BID: Bind_1078
MSL: Msil_1154
MTUN: MTUNDRAET4_0656(hss)
BBAR: RHAL1_00959(hss)
BVR: BVIR_716
BLAG: BLTE_24040
MSC: BN69_1957
PLEO: OHA_1_03491(hss)
MMED: Mame_01224(hss)
HDI: HDIA_4115
JAN: Jann_0956
DSH: Dshi_2947(hss)
LAQU: R2C4_00225
MALG: MALG_02794
LABT: FIU93_17085(hss)
ROH: FIU89_02840(hss)
MALU: KU6B_22460
SHUM: STHU_29010
RCE: RC1_1718(hss)
MAG: amb2266
MGY: MGMSRv2__1441(hss)
MGRY: MSR1_08010(hss)
MAGX: XM1_3089(hss)
MAGN: WV31_07945
ALI: AZOLI_1158(hss)
ABS: AZOBR_140285(hss)
TMO: TMO_3104
HADH: FRZ61_44510(hss)
MAI: MICA_471(hss)
MAN: A11S_454
AMYY: YIM_37955(hss)
OBG: Verru16b_00850(hss)
PPH: Ppha_0878
MBAR: MSBR2_0084
MBAK: MSBR3_0145
MAC: MA_1636
MMA: MM_0166
MMAC: MSMAC_2495
MHOR: MSHOH_1357
MBN: Mboo_1047
 » show all

KEGG   ENZYME: 2.5.1.44
Entry
EC 2.5.1.44                 Enzyme                                 

Name
homospermidine synthase;
putrescine:putrescine 4-aminobutyltransferase (ammonia-forming)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
putrescine/spermidine:putrescine 4-aminobutyltransferase
Reaction(IUBMB)
(1) 2 putrescine = sym-homospermidine + NH3 [RN:R00018];
(2) spermidine + putrescine = sym-homospermidine + propane-1,3-diamine [RN:R01919]
Reaction(KEGG)
R00018 R01919
Substrate
putrescine [CPD:C00134];
spermidine [CPD:C00315]
Product
sym-homospermidine [CPD:C06366];
NH3 [CPD:C00014];
propane-1,3-diamine [CPD:C00986]
Comment
The reaction of this bacterial enzyme occurs in three steps, with some of the intermediates presumably remaining enzyme-bound: (a) NAD+-dependent dehydrogenation of either putrescine or spermidine, forming 4-iminobutan-1-amine or (E)-(4-aminobutylidene)(3-aminopropyl)amine, respectively, (b) attack by water forming 4-aminobutanal (and releasing ammonia or propane-1,3-diamine, respectively), and (c) condensation of 4-aminobutanal with putrescine, which forms homospermidine and restores NAD+. Differs from the eukaryotic enzyme EC 2.5.1.45, homospermidine synthase (spermidine-specific), which cannot use putrescine as donor of the aminobutyl group.
History
EC 2.5.1.44 created 1999, modified 2001
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K00808  homospermidine synthase
Genes
PAU: PA14_35790
PAP: PSPA7_3061
PDK: PADK2_14350
PAES: SCV20265_3124
PAEU: BN889_06565
PST: PSPTO_1868(hss)
PSK: U771_27265
PCZ: PCL1606_20560
PSEM: TO66_21620
PANR: A7J50_5695
SAZ: Sama_3337
SKH: STH12_03268(hss)
AMAL: I607_20267
AMAE: I876_01355
AMAO: I634_01455
AMAI: I635_21559
ASIP: AQUSIP_02550(hss_1) AQUSIP_19380(hss_2)
LPN: lpg2495(hss)
LPH: LPV_2823
LPO: LPO_2687
LPM: LP6_2525(hss)
LPF: lpl2416
LPP: lpp2562
LPC: LPC_1979(hss)
LPA: lpa_03638
LPE: lp12_2487
LLO: LLO_3342(hss)
LHA: LHA_0258(hss) LHA_0415(hss)
LOK: Loa_01233
LCD: clem_13100(hss_1) clem_13880(hss_3)
LLG: 44548918_00308(hss)
LJR: NCTC11533_00329(hss)
LCJ: NCTC11976_00392(hss)
TMC: LMI_1387(hss)
REH: H16_A0043(hss)
CGD: CR3_2929(hss)
CBX: Cenrod_1499(hss)
OFO: BRW83_1089(hss)
BBAG: E1O_25610
PBH: AAW51_2240(hss)
NEU: NE1498(hss)
MLO: mlr3014
MHUA: MCHK_4530
MES: Meso_2727
AMIH: CO731_02153(hss)
PLA: Plav_1354
SME: SMc04016(hss)
SMX: SM11_chr2965(hss)
SMI: BN406_02658(hss)
SMEL: SM2011_c04016(hss)
SMER: DU99_15480
SMD: Smed_2688
SFD: USDA257_c52690(hss)
SAME: SAMCFNEI73_Ch3223(hss)
EAD: OV14_0297
ATU: Atu3768
ARA: Arad_3814(hss)
AVI: Avi_3707
RET: RHE_CH03553(hss)
REL: REMIM1_CH03624(hss)
REP: IE4803_CH03928(hss)
REI: IE4771_CH03866(hss)
RLE: RL4073(hss)
RLG: Rleg_3602
RIR: BN877_II0721(hss)
RHL: LPU83_3541(hss)
RGA: RGR602_CH03450(hss)
RHN: AMJ98_CH03777(hss)
RPHA: AMC79_CH03729(hss)
RHT: NT26_2725(hss)
RHX: AMK02_CH03666(hss)
RHK: Kim5_CH03896(hss)
REZ: AMJ99_CH03830(hss)
NEN: NCHU2750_27630(hss)
SHZ: shn_15290
BMEL: DK63_2103(hss)
BMEE: DK62_2173(hss)
BMF: BAB2_0077
BABO: DK55_3182(hss)
BABR: DO74_2368(hss)
BABT: DK49_2186(hss)
BABB: DK48_2615(hss)
BABU: DK53_3056(hss)
BABS: DK51_2540(hss)
BABC: DO78_3101(hss)
BMS: BRA0078
BSZ: DK67_2110(hss)
BOV: BOV_A0073
BCS: BCAN_B0081(hss)
BCAR: DK60_2965(hss)
BCAS: DA85_10890
BMR: BMI_II80
BPP: BPI_II80
BPV: DK65_2381(hss)
OAN: Oant_4263
OAH: DR92_3986(hss)
OCH: CES85_5524(hss)
BJA: blr7762
BRA: BRADO6264(hss)
BBT: BBta_1345(hss)
BRS: S23_63280
AOL: S58_64330
BRAD: BF49_3189
BRO: BRAD285_6208(hss)
RPA: RPA0865
RPB: RPB_4552
RPC: RPC_4834
RPD: RPD_0850
RPE: RPE_4799
RPT: Rpal_0933
NWI: Nwi_0921
NHA: Nham_3423
OCA: OCAR_4912
OCG: OCA5_c30420(hss)
OCO: OCA4_c29900(hss)
VGO: GJW-30_1_02467(hss)
XAU: Xaut_2138
AZC: AZC_0296
SNO: Snov_3872
MEA: Mex_1p2024(hss)
MDI: METDI2807(hss) METDI4480(hss)
MEX: Mext_2042
MPO: Mpop_2003
MET: M446_0451
MNO: Mnod_1646
MOR: MOC_5748
META: Y590_09740
BID: Bind_1078
MSL: Msil_1154
MTUN: MTUNDRAET4_0656(hss)
BBAR: RHAL1_00959(hss)
BVR: BVIR_716
BLAG: BLTE_24040
MSC: BN69_1957
PLEO: OHA_1_03491(hss)
MMED: Mame_01224(hss)
HDI: HDIA_4115
JAN: Jann_0956
DSH: Dshi_2947(hss)
LAQU: R2C4_00225
MALG: MALG_02794
LABT: FIU93_17085(hss)
ROH: FIU89_02840(hss)
MALU: KU6B_22460
SHUM: STHU_29010
RCE: RC1_1718(hss)
MAG: amb2266
MGY: MGMSRv2__1441(hss)
MGRY: MSR1_08010(hss)
MAGX: XM1_3089(hss)
MAGN: WV31_07945
ALI: AZOLI_1158(hss)
ABS: AZOBR_140285(hss)
TMO: TMO_3104
HADH: FRZ61_44510(hss)
MAI: MICA_471(hss)
MAN: A11S_454
AMYY: YIM_37955(hss)
OBG: Verru16b_00850(hss)
PPH: Ppha_0878
MBAR: MSBR2_0084
MBAK: MSBR3_0145
MAC: MA_1636
MMA: MM_0166
MMAC: MSMAC_2495
MHOR: MSHOH_1357
MBN: Mboo_1047
 » show all
Reference
1  [PMID:437275]
  Authors
Tait GH.
  Title
The formation of homospermidine by an enzyme from Rhodopseudomonas viridis [proceedings]
  Journal
Biochem Soc Trans 7:199-201 (1979)
DOI:10.1042/bst0070199
Reference
2  [PMID:8407874]
  Authors
Yamamoto S, Nagata S, Kusaba K.
  Title
Purification and characterization of homospermidine synthase in Acinetobacter tartarogenes ATCC 31105.
  Journal
J Biochem (Tokyo) 114:45-9 (1993)
DOI:10.1093/oxfordjournals.jbchem.a124137
Reference
3
  Authors
Ober, D., Tholl, D., Martin, W. and Hartmann, T.
  Title
Homospermidine synthase of Rhodopseudomonas viridis: Substrate specificity and effects of the heterologously expressed enzyme on polyamine metabolism of Escherichia coli.
  Journal
J Gen Appl Microbiol 42:411-419 (1996)
Reference
4  [PMID:26776105]
  Authors
Krossa S, Faust A, Ober D, Scheidig AJ
  Title
Comprehensive Structural Characterization of the Bacterial Homospermidine Synthase-an Essential Enzyme of the Polyamine Metabolism.
  Journal
Sci Rep 6:19501 (2016)
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.44
IUBMB Enzyme Nomenclature: 2.5.1.44
ExPASy - ENZYME nomenclature database: 2.5.1.44
BRENDA, the Enzyme Database: 2.5.1.44
CAS: 76106-84-8

KEGG   REACTION: R00018
Entry
R00018                      Reaction                               

Name
putrescine:putrescine 4-aminobutyltransferase (ammonia-forming)
Definition
2 Putrescine <=> sym-Homospermidine + Ammonia
Equation
Comment
homospermidine synthase
Reaction class
RC00053  C00134_C06366
Enzyme
Pathway
rn00960  Tropane, piperidine and pyridine alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K00808  homospermidine synthase [EC:2.5.1.44]
Other DBs
RHEA: 18648

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