| Entry |
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| Name |
homocitrate synthase;
2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase (CoA-acetylating);
acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase;
homocitrate synthetase;
HCS
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| Class |
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
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| Sysname |
acetyl-CoA:2-oxoglutarate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming)
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| Reaction(IUBMB) |
acetyl-CoA + H2O + 2-oxoglutarate = (2R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA [RN: R00271]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
(2R)-2-hydroxybutane-1,2,4-tricarboxylate;
CoA [CPD: C00010]
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| Comment |
Belongs in the alpha-aminoadipate pathway of lysine synthesis, along with EC 4.2.1.36, homoaconitate hydratase. The enzyme also acts with oxaloacetate as substrate, but more slowly [2,3].
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| History |
EC 2.3.3.14 created 1972 as EC 4.1.3.21, transferred 2002 to EC 2.3.3.14
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| Pathway |
| ec01110 | Biosynthesis of secondary metabolites |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K02594 | homocitrate synthase NifV |
| K10977 | methanogen homocitrate synthase |
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| Genes |
» show all
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| Reference |
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| Authors |
Strassman M, Ceci LN. |
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| Title |
Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis. |
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| Journal |
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| Reference |
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| Authors |
Wulandari AP, Miyazaki J, Kobashi N, Nishiyama M, Hoshino T, Yamane H. |
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| Title |
Characterization of bacterial homocitrate synthase involved in lysine biosynthesis. |
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| Journal |
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| Reference |
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| Authors |
Andi B, West AH, Cook PF. |
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| Title |
Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.3.3.14 |
| ExPASy - ENZYME nomenclature database: | 2.3.3.14 |
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