KEGG   ORTHOLOGY: K01795
Entry
K01795                      KO                                     

Name
algG
Definition
mannuronan 5-epimerase [EC:5.1.3.37]
Pathway
ko00051  Fructose and mannose metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00051 Fructose and mannose metabolism
    K01795  algG; mannuronan 5-epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.3  Acting on carbohydrates and derivatives
    5.1.3.37  mannuronan 5-epimerase
     K01795  algG; mannuronan 5-epimerase
Other DBs
RN: R08693
COG: COG3420
Genes
PAE: PA3545(algG)
PAEV: N297_3668(algG)
PAEI: N296_3668(algG)
PAU: PA14_18500(algG)
PAP: PSPA7_1600(algG)
PAG: PLES_14881(algG)
PAF: PAM18_1441(algG)
PNC: NCGM2_4670(algG)
PAEB: NCGM1900_2739(algG)
PDK: PADK2_06640
PAEP: PA1S_07695
PAEM: U769_07170
PAEL: T223_07410
PAEU: BN889_03916(algG)
PAEG: AI22_26210
PAEC: M802_3665(algG)
PAEO: M801_3533(algG)
PMK: MDS_1077
PRE: PCA10_11830(algG) PCA10_38180(algG)
PCQ: PcP3B5_50730(algG)
PPU: PP_1283(algG)
PPF: Pput_4442
PPT: PPS_4408
PPI: YSA_03103
PPX: T1E_3163
PPUH: B479_22175
PPUT: L483_27260
PPUN: PP4_07950(algG)
PPUD: DW66_4799
PMON: X969_21710
PMOT: X970_21345
PST: PSPTO_1238(algG)
PSB: Psyr_1058
PSYR: N018_20380
PSP: PSPPH_1113(algG)
PVD: CFBP1590__4282(algG)
PFL: PFL_1019(algG)
PPRC: PFLCHA0_c10390(algG)
PPRO: PPC_1057
PFO: Pfl01_0955(algG)
PFS: PFLU_0985(algG)
PFC: PflA506_0965(algG)
PFB: VO64_4066
PMAN: OU5_2452
PFW: PF1751_v1c09430(algG)
PEN: PSEEN3463 PSEEN4540(algG)
PPUU: PputUW4_04476(algG)
PKC: PKB_0963(algG)
PSES: PSCI_0726
PSEM: TO66_05185
PSOS: POS17_1025
PANR: A7J50_0987
PSIL: PMA3_24610
AVN: Avin_10920(algG)
AVL: AvCA_10920(algG)
AVD: AvCA6_10920(algG)
ACX: Achr_31230(algG)
ABO: ABO_0394(algG)
PLG: NCTC10937_04288(algG)
NIS: NIS_0188
MMED: Mame_02308(algG)
MALG: MALG_02780
SECH: B18_00690
SEN: SACE_3182
CBAL: M667_16165
CBAT: M666_16150
NEV: NTE_02401
TAA: NMY3_01609(algG)
NFN: NFRAN_2449(algG)
 » show all
Reference
PMID:8144447
  Authors
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE
  Title
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.
  Journal
J Bacteriol 176:1821-30 (1994)
DOI:10.1128/JB.176.7.1821-1830.1994
  Sequence
[pae:PA3545]

KEGG   ENZYME: 5.1.3.37
Entry
EC 5.1.3.37                 Enzyme                                 

Name
mannuronan 5-epimerase;
algG (gene name);
alginate epimerase;
C5-mannuronan epimerase;
mannuronan C-5-epimerase
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
Sysname
[mannuronan]-beta-D-mannuronate 5-epimerase
Reaction(IUBMB)
[mannuronan]-beta-D-mannuronate = [alginate]-alpha-L-guluronate
Reaction(KEGG)
(other) R08693
Substrate
[mannuronan]-beta-D-mannuronate
Product
[alginate]-alpha-L-guluronate
Comment
The enzyme epimerizes the C-5 bond in some beta-D-mannuronate residues in mannuronan, converting them to alpha-L-guluronate residues, and thus modifying the mannuronan into alginate. It is found in brown algae and alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
History
EC 5.1.3.37 created 2015
Pathway
ec00051  Fructose and mannose metabolism
ec01100  Metabolic pathways
Orthology
K01795  mannuronan 5-epimerase
Genes
PAE: PA3545(algG)
PAEV: N297_3668(algG)
PAEI: N296_3668(algG)
PAU: PA14_18500(algG)
PAP: PSPA7_1600(algG)
PAG: PLES_14881(algG)
PAF: PAM18_1441(algG)
PNC: NCGM2_4670(algG)
PAEB: NCGM1900_2739(algG)
PDK: PADK2_06640
PAEP: PA1S_07695
PAEM: U769_07170
PAEL: T223_07410
PAEU: BN889_03916(algG)
PAEG: AI22_26210
PAEC: M802_3665(algG)
PAEO: M801_3533(algG)
PMK: MDS_1077
PRE: PCA10_11830(algG) PCA10_38180(algG)
PCQ: PcP3B5_50730(algG)
PPU: PP_1283(algG)
PPF: Pput_4442
PPT: PPS_4408
PPI: YSA_03103
PPX: T1E_3163
PPUH: B479_22175
PPUT: L483_27260
PPUN: PP4_07950(algG)
PPUD: DW66_4799
PMON: X969_21710
PMOT: X970_21345
PST: PSPTO_1238(algG)
PSB: Psyr_1058
PSYR: N018_20380
PSP: PSPPH_1113(algG)
PVD: CFBP1590__4282(algG)
PFL: PFL_1019(algG)
PPRC: PFLCHA0_c10390(algG)
PPRO: PPC_1057
PFO: Pfl01_0955(algG)
PFS: PFLU_0985(algG)
PFC: PflA506_0965(algG)
PFB: VO64_4066
PMAN: OU5_2452
PFW: PF1751_v1c09430(algG)
PEN: PSEEN3463 PSEEN4540(algG)
PPUU: PputUW4_04476(algG)
PKC: PKB_0963(algG)
PSES: PSCI_0726
PSEM: TO66_05185
PSOS: POS17_1025
PANR: A7J50_0987
PSIL: PMA3_24610
AVN: Avin_10920(algG)
AVL: AvCA_10920(algG)
AVD: AvCA6_10920(algG)
ACX: Achr_31230(algG)
ABO: ABO_0394(algG)
PLG: NCTC10937_04288(algG)
NIS: NIS_0188
MMED: Mame_02308(algG)
MALG: MALG_02780
SECH: B18_00690
SEN: SACE_3182
CBAL: M667_16165
CBAT: M666_16150
NEV: NTE_02401
TAA: NMY3_01609(algG)
NFN: NFRAN_2449(algG)
 » show all
Reference
1  [PMID:8144447]
  Authors
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE
  Title
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.
  Journal
J Bacteriol 176:1821-30 (1994)
DOI:10.1128/JB.176.7.1821-1830.1994
  Sequence
[pae:PA3545]
Reference
2  [PMID:11707327]
  Authors
Morea A, Mathee K, Franklin MJ, Giacomini A, O'Regan M, Ohman DE
  Title
Characterization of algG encoding C5-epimerase in the alginate biosynthetic gene  cluster of Pseudomonas fluorescens.
  Journal
Gene 278:107-14 (2001)
DOI:10.1016/S0378-1119(01)00685-0
Reference
3  [PMID:14526115]
  Authors
Nyvall P, Corre E, Boisset C, Barbeyron T, Rousvoal S, Scornet D, Kloareg B, Boyen C
  Title
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria  digitata.
  Journal
Plant Physiol 133:726-35 (2003)
DOI:10.1104/pp.103.025981
Reference
4  [PMID:12581364]
  Authors
Jain S, Franklin MJ, Ertesvag H, Valla S, Ohman DE
  Title
The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa.
  Journal
Mol Microbiol 47:1123-33 (2003)
DOI:10.1046/j.1365-2958.2003.03361.x
Reference
5  [PMID:15968068]
  Authors
Douthit SA, Dlakic M, Ohman DE, Franklin MJ
  Title
Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix.
  Journal
J Bacteriol 187:4573-83 (2005)
DOI:10.1128/JB.187.13.4573-4583.2005
Reference
6  [PMID:24398681]
  Authors
Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codee JD, Klassen JS, Howell PL
  Title
Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG.
  Journal
J Biol Chem 289:6006-19 (2014)
DOI:10.1074/jbc.M113.533158
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.37
IUBMB Enzyme Nomenclature: 5.1.3.37
ExPASy - ENZYME nomenclature database: 5.1.3.37
BRENDA, the Enzyme Database: 5.1.3.37

KEGG   REACTION: R08693
Entry
R08693                      Reaction                               

Name
Poly(beta-D-mannuronate) C5-epimerase
Definition
(beta-D-Mannuronate)n <=> (Alginate)n
Equation
Reaction class
RC00509  C01768_C05144
Enzyme
Pathway
rn00051  Fructose and mannose metabolism
rn01100  Metabolic pathways
Orthology
K01795  mannuronan 5-epimerase [EC:5.1.3.37]
Other DBs
RHEA: 45575

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