KEGG   ORTHOLOGY: K03333Help
Entry
K03333                      KO                                     

Name
choD
Definition
cholesterol oxidase [EC:1.1.3.6]
Pathway
ko00984  Steroid degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K03333  choD; cholesterol oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.3  With oxygen as acceptor
    1.1.3.6  cholesterol oxidase
     K03333  choD; cholesterol oxidase
BRITE hierarchy
Other DBs
RN: R01459
GO: 0016995
Genes
AHN: NCTC12129_02598(choD_1) NCTC12129_02600(choD_2)
SFO: Z042_05240
LUE: DCD74_03480
DYE: EO087_09985
LRZ: BJI69_21015
VOW: A9237_01175
PFO: Pfl01_5219
PFE: PSF113_5443
PMAN: OU5_3960
PCG: AXG94_13095
PSOS: POS17_5672
ACX: Achr_21660(choD)
PSYC: DABAL43B_0238(pteG)
PSYA: AOT82_644
ACB: A1S_1119
ABY: ABAYE2620
ABN: AB57_1203
ABB: ABBFA_02393(choD)
ABAZ: P795_11715
ABAU: IX87_05230
ABAA: IX88_07845
ACC: BDGL_000403(choB)
AUG: URS_2767
PSEN: PNC201_17550(choD)
MVS: MVIS_1634
CJA: CJA_0537(choB)
LLG: 44548918_01159(choD)
HCH: HCH_01642
HBE: BEI_3106(choD)
ABO: ABO_1259
ADI: B5T_02424
APAC: S7S_08800
AXE: P40_11770
CVI: CV_2218
RSO: RSp1167
RSE: F504_4662
REH: H16_A1655(betA3)
CNC: CNE_1c16780(betA2)
BMA: BMAA2022
BMAL: DM55_3781
BMAE: DM78_4398
BMAQ: DM76_4478
BMAI: DM57_06010
BMAF: DM51_3693
BMAZ: BM44_3740
BMAB: BM45_4516
BPS: BPSS2278
BPSE: BDL_5753
BPSM: BBQ_3826
BPSU: BBN_5774
BPSD: BBX_4921
BPK: BBK_5058
BPSH: DR55_5452
BPSA: BBU_3711
BPSO: X996_5223
BUT: X994_4772
BTQ: BTQ_5597
BTJ: BTJ_4252
BTZ: BTL_5062
BTV: BTHA_5160
BTHE: BTN_4720
BTHM: BTRA_4986
BTHA: DR62_4827
BTHL: BG87_5054
BOK: DM82_4050
BOC: BG90_3977
BVE: AK36_5548
BUB: BW23_3407
BXE: Bxe_B1816
BXB: DR64_7122
BPH: Bphy_5113
BFN: OI25_4267
HYF: DTO96_101266(choA)
RFR: Rfer_0318
LCH: Lcho_3191
DAL: Dalk_4295
DWD: DSCW_59550(choB)
ADE: Adeh_3081
SCL: sce0787
HOH: Hoch_5037
BBA: Bd0736(choA) Bd2646(choD)
BBAT: Bdt_0702(choA) Bdt_2561(choD)
BMX: BMS_1931(choD)
AVI: Avi_3364
MCG: GL4_0515
SSAN: NX02_01130
SHUM: STHU_37530
MTU: Rv3409c(choD)
MTC: MT3517
MRA: MRA_3449(choD)
MTUR: CFBS_3613(choD)
MTO: MTCTRI2_3481(choD)
MTD: UDA_3409c(choD)
MTN: ERDMAN_3730(choD)
MTUC: J113_23855
MTUE: J114_18265
MTUL: TBHG_03346
MTUT: HKBT1_3600(choD)
MTUU: HKBT2_3607(choD)
MTQ: HKBS1_3610(choD)
MBO: BQ2027_MB3443C(choD)
MBB: BCG_3479c(choD)
MBT: JTY_3479(choD)
MBM: BCGMEX_3477c(choD)
MBX: BCGT_3281
MAF: MAF_34230(choD)
MMIC: RN08_3762
MCE: MCAN_34331(choD)
MCQ: BN44_80068(choD)
MCV: BN43_70062(choD)
MCX: BN42_50067(choD)
MCZ: BN45_70054(choD)
MLE: ML0389(choD)
MLB: MLBr00389(choD)
MAV: MAV_4354
MIT: OCO_42200
MIA: OCU_42130
MID: MIP_06364
MYO: OEM_42560
MIR: OCQ_43470
MLP: MLM_3514
MUL: MUL_0903(choD)
MMC: Mmcs_1170
MKM: Mkms_1187
MJL: Mjls_1197
MMI: MMAR_1140(choD)
MMAE: MMARE11_10990(choD)
MMM: W7S_21155
MLI: MULP_01281(choD)
MHAD: B586_18165
MSHG: MSG_01041(choD)
MSG: MSMEI_1565(choD)
MVA: Mvan_1514
MPHL: MPHLCCUG_01453(choD)
MVQ: MYVA_1266
MTHN: 4412656_00282(choA) 4412656_00477(choD_1) 4412656_01056(choD_2)
MHAS: MHAS_00386(choD)
MAB: MAB_3719c
MSAL: DSM43276_01094(choD_1) DSM43276_03517(choD_2)
MJD: JDM601_0848(choD) JDM601_3221(choD)
MTER: 4434518_00810(choD_1) 4434518_03208(choD_4)
CJK: jk0629(choE)
CUR: cu1318
CUA: CU7111_1299(choE)
CRD: CRES_0700(choD)
CVA: CVAR_1073
CGY: CGLY_10185(choD)
COA: DR71_1512(choD)
NFR: ERS450000_01481(choD_1) ERS450000_01614(choD_2) ERS450000_02934(choA_1) ERS450000_03933(choA_2) ERS450000_05565(choD_4)
RFA: A3L23_00184(choD_1) A3L23_01299(choA_1) A3L23_01313(choB) A3L23_02371(choD_2) A3L23_02942(choA_2)
RHS: A3Q41_00381(choA_1) A3Q41_00448(choD_1) A3Q41_00996(choD_2) A3Q41_02056(choA_2) A3Q41_03230(choD_3)
RHU: A3Q40_00355(choA) A3Q40_01763(choB) A3Q40_02219(choD_1) A3Q40_03498(choD_2)
RRT: 4535765_00444(choD_1) 4535765_00558(choA_1) 4535765_00723(choA_2) 4535765_00837(choA_3) 4535765_00906(choD_2) 4535765_03467(choD_4)
GRU: GCWB2_05790(choD1) GCWB2_08510(choD2) GCWB2_13315(choB) GCWB2_21170(choD5) GCWB2_22830(choA)
GOM: D7316_00928(choB_1) D7316_00929(choA) D7316_02214(choD_1) D7316_02835(choD_2) D7316_03176(choB_2)
TPR: Tpau_0908
SRT: Srot_2942
SCO: SCO4781(SCD63.13)
SALB: XNR_3818
SMA: SAVERM_5017(choD)
SCT: SCAT_3673
SBH: SBI_04801(choD) SBI_06081
SHY: SHJG_5880
SDV: BN159_3636(choD) BN159_6302(choA)
SLV: SLIV_14455(choD)
SAMB: SAM23877_4595(choD)
SRW: TUE45_05301(choD)
SLE: sle_28920(sle_28920) sle_59900(sle_59900)
SRN: A4G23_03453(choD)
STRD: NI25_15015
SMAL: SMALA_3374
SLX: SLAV_02815(choA1) SLAV_04375(choB) SLAV_15530(choD) SLAV_26540(choA2)
SGE: DWG14_03354(choD) DWG14_06198(choA)
KSK: KSE_30980(choD)
LXL: KDY119_00245(choD)
ART: Arth_1255
XCE: Xcel_2031
CFL: Cfla_3546
DCO: SAMEA4475696_0485(choD)
NCA: Noca_3632
NDK: I601_0323(choD)
TFU: Tfu_2332
NDA: Ndas_0464
NAL: B005_3333(choD)
STRR: EKD16_19755(choD)
FAL: FRAAL6768(choA)
NML: Namu_2922
SEN: SACE_6417(pteG) SACE_6705(choD)
SACC: EYD13_02650(choD) EYD13_17010(choA)
AJA: AJAP_05245(choA) AJAP_12575(choD1) AJAP_18180(choB) AJAP_31290(choD2) AJAP_35850(choD3)
AMQ: AMETH_0745(choD) AMETH_1909(pteG) AMETH_3345(choD) AMETH_5766(pteG) AMETH_6096(choD)
AMYY: YIM_25865(choA1) YIM_36455(choB) YIM_39985(choD1) YIM_43735(choD2)
PSEA: WY02_04695
PSEH: XF36_12045
PAUT: Pdca_10940
SESP: BN6_33640 BN6_78530(choD)
ACTI: UA75_11025
ACAD: UA74_10940
AHG: AHOG_10280(choD)
ASE: ACPL_5525(choD) ACPL_7034(choD)
ACTN: L083_2883 L083_6596(choD)
AFS: AFR_34765
TBI: Tbis_0628
CWO: Cwoe_4226
NSH: GXM_09575
CALH: IJ00_26400
PBF: CFX0092_B0471(choD)
MFF: MFFC18_09650(choB)
PLS: VT03_10745(choD)
LIC: LIC_13194(choB) LIC_13195
LBL: LBL_0185
LBI: LEPBI_I0286(choB)
LBF: LBF_0277
ABAS: ACPOL_1081
SUS: Acid_5726
ABAC: LuPra_02327(choA)
FLM: MY04_1104
CHZ: CHSO_0975(choA)
TJE: TJEJU_0790(choD)
KAN: IMCC3317_44990(choD)
CABY: Cabys_1692
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Brzostek A, Dziadek B, Rumijowska-Galewicz A, Pawelczyk J, Dziadek J
  Title
Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis.
  Journal
FEMS Microbiol Lett 275:106-12 (2007)
DOI:10.1111/j.1574-6968.2007.00865.x
  Sequence
[mtu:Rv3409c]

KEGG   ORTHOLOGY: K16045Help
Entry
K16045                      KO                                     

Name
hsd
Definition
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]
Pathway
ko00984  Steroid degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16045  hsd; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.145  3beta-hydroxy-Delta5-steroid dehydrogenase
     K16045  hsd; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 5. Isomerases
  5.3  Intramolecular oxidoreductases
   5.3.3  Transposing C=C bonds
    5.3.3.1  steroid Delta-isomerase
     K16045  hsd; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
BRITE hierarchy
Other DBs
RN: R09920
COG: COG0451
GO: 0003854 0004769
Genes
MTU: Rv1106c
MTV: RVBD_1106c
MTC: MT1137
MRA: MRA_1117
MTF: TBFG_11128
MTB: TBMG_02877
MTK: TBSG_02893
MTZ: TBXG_002857
MTG: MRGA327_06925
MTI: MRGA423_06915
MTUR: CFBS_1174
MTD: UDA_1106c
MTUC: J113_07785
MTUE: J114_05980
MTUH: I917_07855
MTUL: TBHG_01091
MTUT: HKBT1_1172
MTUU: HKBT2_1178
MBB: BCG_1166c
MBT: JTY_1139
MBX: BCGT_0934
MAF: MAF_11210
MMIC: RN08_1242
MLE: ML1942
MLB: MLBr01942
MPA: MAP_2688
MAO: MAP4_1128
MAVI: RC58_05605
MAVU: RE97_05600
MAV: MAV_1225
MIT: OCO_11340
MIA: OCU_11320
MID: MIP_01824
MYO: OEM_11450
MIR: OCQ_11350
MLP: MLM_1173
MUL: MUL_0172
MMC: Mmcs_4109
MKM: Mkms_4185
MJL: Mjls_4340
MMI: MMAR_4359
MMM: W7S_05545
MHAD: B586_07090
MSHG: MSG_03923
MVA: Mvan_4635
MGI: Mflv_2075
MVQ: MYVA_4444
MHAS: MHAS_03213
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Yang X, Dubnau E, Smith I, Sampson NS
  Title
Rv1106c from Mycobacterium tuberculosis is a 3beta-hydroxysteroid dehydrogenase.
  Journal
Biochemistry 46:9058-67 (2007)
DOI:10.1021/bi700688x
  Sequence
[mtu:Rv1106c]
Reference
  Authors
Yang X, Gao J, Smith I, Dubnau E, Sampson NS
  Title
Cholesterol is not an essential source of nutrition for Mycobacterium tuberculosis during infection.
  Journal
J Bacteriol 193:1473-6 (2011)
DOI:10.1128/JB.01210-10
  Sequence
[mtu:Rv1106c]

KEGG   ENZYME: 1.1.1.145Help
Entry
EC 1.1.1.145                Enzyme                                 

Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]
Product
3-oxo-Delta5-steroid [CPD:C01034];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
ec00140  Steroid hormone biosynthesis
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA: 3283(HSD3B1) 3284(HSD3B2)
PTR: 457165 469446
PPS: 100982464(HSD3B2) 100983009 100990187
GGO: 101141070 101141801 101146563(HSD3B2)
PON: 100451655 100452394
NLE: 100596087 100605995(HSD3B2) 100606563 100607955
MCC: 712686(HSD3B2) 713091
MCF: 102129497 102133592(HSD3B1) 102134747 102135110
CSAB: 103224104 103224109(HSD3B2)
RRO: 104654599 104654602 104654605
MMU: 100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
HGL: 101707488(Hsd3b1) 101707858
TUP: 102470981
CFA: 483146(HSD3B2)
VVP: 112916789
AML: 100464280(HSD3B1)
UMR: 103677123
UAH: 113244362
ORO: 101378904
ELK: 111143436
FCA: 100616070(HSD3B2)
PTG: 102957262
PPAD: 109272160
AJU: 106978496
BTA: 281824(HSD3B1)
BOM: 102274088
BIU: 109555859
BBUB: 102413429
CHX: 100860869(3BHSD)
OAS: 100190978(HSD3B1)
SSC: 445539(HSD3B1)
CFR: 102512447
CDK: 105089475
BACU: 103015346
LVE: 103083046
OOR: 101284283
DLE: 111179902
PCAD: 102987751
ECB: 100034078(HSD3B2)
EPZ: 103548379
EAI: 106832920(HSD3B2)
MYB: 106723550(HSD3B2)
MNA: 107545288(HSD3B2)
HAI: 109389868(HSD3B2)
DRO: 112314004(HSD3B2)
PALE: 102891879
RAY: 107518830(HSD3B2)
MJV: 108390800
LAV: 100659049(HSD3B2)
TMU: 101358325
MDO: 100012668
SHR: 100913530(HSD3B2)
PCW: 110219672(HSD3B2)
OAA: 100080135
GGA: 396015(HSD3B1)
MGP: 104915630
CJO: 107319373(3beta-HSD)
NMEL: 110398845
APLA: 101803865
ACYG: 106045741
TGU: 751770(HSD3B1)
LSR: 110480744
SCAN: 103827136
GFR: 102039409
FAB: 101817697
PHI: 102101526
PMAJ: 107211459
CCAE: 111938581
CCW: 104687106
ETL: 114058198
FPG: 101921564
FCH: 102052697
CLV: 102087460
EGZ: 104126518
NNI: 104008615
ACUN: 113485872
PADL: 103921223
AAM: 106497227
ASN: 102367786(HSD3B1)
AMJ: 109280168(HSD3B1)
PSS: 102453952
CMY: 102934871
CPIC: 101946936
ACS: 100561899(hsd3b1)
PVT: 110076926
PBI: 103067418
PMUR: 107285816
TSR: 106542700
PMUA: 114587929(HSD3B2)
GJA: 107114856
XLA: 734818(hsd3b1.L)
XTR: 100490622
NPR: 108802532
DRE: 373131(hsd3b2) 553532(hsd3b1)
SRX: 107712267
SANH: 107658243
SGH: 107569293
CCAR: 109052439
IPU: 100304475(HSD3B)
PHYP: 113524942(hsd3b1)
AMEX: 103027852(hsd3b1)
EEE: 113587753
TRU: 101069884(hsd3b1)
LCO: 104921488(hsd3b1)
MZE: 101485719
ONL: 100534568
OLA: 100144358(3bhsd)
XMA: 102228303
XCO: 114147930
PRET: 103477979
CVG: 107087352
NFU: 107389612(hsd3b1)
KMR: 108244192(hsd3b1)
ALIM: 106531920
AOCE: 111570570(hsd3b1)
CSEM: 103391994(hsd3b1)
POV: 109637945
LCF: 108902437
SDU: 111218347
SLAL: 111652102
HCQ: 109522071
BPEC: 110156192(hsd3b1)
MALB: 109951646
SASA: 106586600
OTW: 112240651
SALP: 111978723
ELS: 105016430
SFM: 108937872
PKI: 111860692
LCM: 102345588
CMK: 103180179
RTP: 109918392
DME: Dmel_CG7724(CG7724)
DER: 6543694
DSI: Dsimw501_GD14693(Dsim_GD14693)
DPE: 6603899
DMN: 108165559
DWI: 6645028
DAZ: 108612818
DNV: 108651018
DHE: 111602930
MDE: 101899660
LCQ: 111691019
AAG: 5566270
NVI: 100120136
CSOL: 105366624
CLEC: 106661338
ZNE: 110831195
FCD: 110862702
PVM: 113815316
TUT: 107363285
DPTE: 113794090
CSCU: 111622487
PTEP: 107437550
LAK: 106151343
MTU: Rv1106c
MTC: MT1137
MRA: MRA_1117
MTUR: CFBS_1174
MTD: UDA_1106c
MTUC: J113_07785
MTUE: J114_05980
MTUH: I917_07855
MTUL: TBHG_01091
MTUT: HKBT1_1172
MTUU: HKBT2_1178
MBB: BCG_1166c
MBT: JTY_1139
MBX: BCGT_0934
MAF: MAF_11210
MMIC: RN08_1242
MLE: ML1942
MLB: MLBr01942
MPA: MAP_2688
MAO: MAP4_1128
MAVI: RC58_05605
MAVU: RE97_05600
MAV: MAV_1225
MIT: OCO_11340
MIA: OCU_11320
MID: MIP_01824
MYO: OEM_11450
MIR: OCQ_11350
MLP: MLM_1173
MUL: MUL_0172
MMC: Mmcs_4109
MKM: Mkms_4185
MJL: Mjls_4340
MMI: MMAR_4359
MMM: W7S_05545
MHAD: B586_07090
MSHG: MSG_03923
MVA: Mvan_4635
MGI: Mflv_2075
MVQ: MYVA_4444
MHAS: MHAS_03213
 » show all
Taxonomy
Reference
1  [PMID:4226148]
  Authors
Cheatum SG, Watten JC.
  Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
  Journal
Biochim Biophys Acta 122:1-13 (1966)
Reference
2  [PMID:14220672]
  Authors
KORITZ SB.
  Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
  Journal
Biochemistry 3:1098-102 (1964)
Reference
3
  Authors
Neville, A.M., Orr, J.C. and Engel, L.L.
  Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
  Journal
Biochem J 107:20 (1968)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.145
IUBMB Enzyme Nomenclature: 1.1.1.145
ExPASy - ENZYME nomenclature database: 1.1.1.145
BRENDA, the Enzyme Database: 1.1.1.145
CAS: 9044-85-3

KEGG   ENZYME: 1.1.3.6Help
Entry
EC 1.1.3.6                  Enzyme                                 

Name
cholesterol oxidase;
cholesterol- O2 oxidoreductase;
3beta-hydroxy steroid oxidoreductase;
3beta-hydroxysteroid:oxygen oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
cholesterol:oxygen oxidoreductase
Reaction(IUBMB)
cholesterol + O2 = cholest-5-en-3-one + H2O2 [RN:R01459]
Reaction(KEGG)
Substrate
cholesterol [CPD:C00187];
O2 [CPD:C00007]
Product
cholest-5-en-3-one;
H2O2 [CPD:C00027]
Comment
Contains FAD. Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyse the first two steps in the degradation of cholesterol. The enzyme catalyses the oxidation of the 3beta-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Delta5 position to Delta6 position (cf. EC 5.3.3.1, steroid Delta-isomerase).
History
EC 1.1.3.6 created 1961, modified 1982, modified 2012
Pathway
ec00984  Steroid degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03333  cholesterol oxidase
Genes
AHN: NCTC12129_02598(choD_1) NCTC12129_02600(choD_2)
SFO: Z042_05240
LUE: DCD74_03480
DYE: EO087_09985
LRZ: BJI69_21015
VOW: A9237_01175
PFO: Pfl01_5219
PFE: PSF113_5443
PMAN: OU5_3960
PCG: AXG94_13095
PSOS: POS17_5672
ACX: Achr_21660(choD)
PSYC: DABAL43B_0238(pteG)
PSYA: AOT82_644
ACB: A1S_1119
ABY: ABAYE2620
ABN: AB57_1203
ABB: ABBFA_02393(choD)
ABAZ: P795_11715
ABAU: IX87_05230
ABAA: IX88_07845
ACC: BDGL_000403(choB)
AUG: URS_2767
PSEN: PNC201_17550(choD)
MVS: MVIS_1634
CJA: CJA_0537(choB)
LLG: 44548918_01159(choD)
HCH: HCH_01642
HBE: BEI_3106(choD)
ABO: ABO_1259
ADI: B5T_02424
APAC: S7S_08800
AXE: P40_11770
CVI: CV_2218
RSO: RSp1167
RSE: F504_4662
REH: H16_A1655(betA3)
CNC: CNE_1c16780(betA2)
BMA: BMAA2022
BMAL: DM55_3781
BMAE: DM78_4398
BMAQ: DM76_4478
BMAI: DM57_06010
BMAF: DM51_3693
BMAZ: BM44_3740
BMAB: BM45_4516
BPS: BPSS2278
BPSE: BDL_5753
BPSM: BBQ_3826
BPSU: BBN_5774
BPSD: BBX_4921
BPK: BBK_5058
BPSH: DR55_5452
BPSA: BBU_3711
BPSO: X996_5223
BUT: X994_4772
BTQ: BTQ_5597
BTJ: BTJ_4252
BTZ: BTL_5062
BTV: BTHA_5160
BTHE: BTN_4720
BTHM: BTRA_4986
BTHA: DR62_4827
BTHL: BG87_5054
BOK: DM82_4050
BOC: BG90_3977
BVE: AK36_5548
BUB: BW23_3407
BXE: Bxe_B1816
BXB: DR64_7122
BPH: Bphy_5113
BFN: OI25_4267
HYF: DTO96_101266(choA)
RFR: Rfer_0318
LCH: Lcho_3191
DAL: Dalk_4295
DWD: DSCW_59550(choB)
ADE: Adeh_3081
SCL: sce0787
HOH: Hoch_5037
BBA: Bd0736(choA) Bd2646(choD)
BBAT: Bdt_0702(choA) Bdt_2561(choD)
BMX: BMS_1931(choD)
AVI: Avi_3364
MCG: GL4_0515
SSAN: NX02_01130
SHUM: STHU_37530
MTU: Rv3409c(choD)
MTC: MT3517
MRA: MRA_3449(choD)
MTUR: CFBS_3613(choD)
MTO: MTCTRI2_3481(choD)
MTD: UDA_3409c(choD)
MTN: ERDMAN_3730(choD)
MTUC: J113_23855
MTUE: J114_18265
MTUL: TBHG_03346
MTUT: HKBT1_3600(choD)
MTUU: HKBT2_3607(choD)
MTQ: HKBS1_3610(choD)
MBO: BQ2027_MB3443C(choD)
MBB: BCG_3479c(choD)
MBT: JTY_3479(choD)
MBM: BCGMEX_3477c(choD)
MBX: BCGT_3281
MAF: MAF_34230(choD)
MMIC: RN08_3762
MCE: MCAN_34331(choD)
MCQ: BN44_80068(choD)
MCV: BN43_70062(choD)
MCX: BN42_50067(choD)
MCZ: BN45_70054(choD)
MLE: ML0389(choD)
MLB: MLBr00389(choD)
MAV: MAV_4354
MIT: OCO_42200
MIA: OCU_42130
MID: MIP_06364
MYO: OEM_42560
MIR: OCQ_43470
MLP: MLM_3514
MUL: MUL_0903(choD)
MMC: Mmcs_1170
MKM: Mkms_1187
MJL: Mjls_1197
MMI: MMAR_1140(choD)
MMAE: MMARE11_10990(choD)
MMM: W7S_21155
MLI: MULP_01281(choD)
MHAD: B586_18165
MSHG: MSG_01041(choD)
MSG: MSMEI_1565(choD)
MVA: Mvan_1514
MPHL: MPHLCCUG_01453(choD)
MVQ: MYVA_1266
MTHN: 4412656_00282(choA) 4412656_00477(choD_1) 4412656_01056(choD_2)
MHAS: MHAS_00386(choD)
MAB: MAB_3719c
MSAL: DSM43276_01094(choD_1) DSM43276_03517(choD_2)
MJD: JDM601_0848(choD) JDM601_3221(choD)
MTER: 4434518_00810(choD_1) 4434518_03208(choD_4)
CJK: jk0629(choE)
CUR: cu1318
CUA: CU7111_1299(choE)
CRD: CRES_0700(choD)
CVA: CVAR_1073
CGY: CGLY_10185(choD)
COA: DR71_1512(choD)
NFR: ERS450000_01481(choD_1) ERS450000_01614(choD_2) ERS450000_02934(choA_1) ERS450000_03933(choA_2) ERS450000_05565(choD_4)
RFA: A3L23_00184(choD_1) A3L23_01299(choA_1) A3L23_01313(choB) A3L23_02371(choD_2) A3L23_02942(choA_2)
RHS: A3Q41_00381(choA_1) A3Q41_00448(choD_1) A3Q41_00996(choD_2) A3Q41_02056(choA_2) A3Q41_03230(choD_3)
RHU: A3Q40_00355(choA) A3Q40_01763(choB) A3Q40_02219(choD_1) A3Q40_03498(choD_2)
RRT: 4535765_00444(choD_1) 4535765_00558(choA_1) 4535765_00723(choA_2) 4535765_00837(choA_3) 4535765_00906(choD_2) 4535765_03467(choD_4)
GRU: GCWB2_05790(choD1) GCWB2_08510(choD2) GCWB2_13315(choB) GCWB2_21170(choD5) GCWB2_22830(choA)
GOM: D7316_00928(choB_1) D7316_00929(choA) D7316_02214(choD_1) D7316_02835(choD_2) D7316_03176(choB_2)
TPR: Tpau_0908
SRT: Srot_2942
SCO: SCO4781(SCD63.13)
SALB: XNR_3818
SMA: SAVERM_5017(choD)
SCT: SCAT_3673
SBH: SBI_04801(choD) SBI_06081
SHY: SHJG_5880
SDV: BN159_3636(choD) BN159_6302(choA)
SLV: SLIV_14455(choD)
SAMB: SAM23877_4595(choD)
SRW: TUE45_05301(choD)
SLE: sle_28920(sle_28920) sle_59900(sle_59900)
SRN: A4G23_03453(choD)
STRD: NI25_15015
SMAL: SMALA_3374
SLX: SLAV_02815(choA1) SLAV_04375(choB) SLAV_15530(choD) SLAV_26540(choA2)
SGE: DWG14_03354(choD) DWG14_06198(choA)
KSK: KSE_30980(choD)
LXL: KDY119_00245(choD)
ART: Arth_1255
XCE: Xcel_2031
CFL: Cfla_3546
DCO: SAMEA4475696_0485(choD)
NCA: Noca_3632
NDK: I601_0323(choD)
TFU: Tfu_2332
NDA: Ndas_0464
NAL: B005_3333(choD)
STRR: EKD16_19755(choD)
FAL: FRAAL6768(choA)
NML: Namu_2922
SEN: SACE_6417(pteG) SACE_6705(choD)
SACC: EYD13_02650(choD) EYD13_17010(choA)
AJA: AJAP_05245(choA) AJAP_12575(choD1) AJAP_18180(choB) AJAP_31290(choD2) AJAP_35850(choD3)
AMQ: AMETH_0745(choD) AMETH_1909(pteG) AMETH_3345(choD) AMETH_5766(pteG) AMETH_6096(choD)
AMYY: YIM_25865(choA1) YIM_36455(choB) YIM_39985(choD1) YIM_43735(choD2)
PSEA: WY02_04695
PSEH: XF36_12045
PAUT: Pdca_10940
SESP: BN6_33640 BN6_78530(choD)
ACTI: UA75_11025
ACAD: UA74_10940
AHG: AHOG_10280(choD)
ASE: ACPL_5525(choD) ACPL_7034(choD)
ACTN: L083_2883 L083_6596(choD)
AFS: AFR_34765
TBI: Tbis_0628
CWO: Cwoe_4226
NSH: GXM_09575
CALH: IJ00_26400
PBF: CFX0092_B0471(choD)
MFF: MFFC18_09650(choB)
PLS: VT03_10745(choD)
LIC: LIC_13194(choB) LIC_13195
LBL: LBL_0185
LBI: LEPBI_I0286(choB)
LBF: LBF_0277
ABAS: ACPOL_1081
SUS: Acid_5726
ABAC: LuPra_02327(choA)
FLM: MY04_1104
CHZ: CHSO_0975(choA)
TJE: TJEJU_0790(choD)
KAN: IMCC3317_44990(choD)
CABY: Cabys_1692
 » show all
Taxonomy
Reference
1  [PMID:4757363]
  Authors
Richmond W.
  Title
Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.
  Journal
Clin Chem 19:1350-6 (1973)
Reference
2  [PMID:13143010]
  Authors
STADTMAN TC, CHERKES A, ANFINSEN CB.
  Title
Studies on the microbiological degradation of cholesterol.
  Journal
J Biol Chem 206:511-23 (1954)
Reference
3  [PMID:10822008]
  Authors
MacLachlan J, Wotherspoon AT, Ansell RO, Brooks CJ
  Title
Cholesterol oxidase: sources, physical properties and analytical applications.
  Journal
J Steroid Biochem Mol Biol 72:169-95 (2000)
DOI:10.1016/S0960-0760(00)00044-3
Reference
4  [PMID:20213543]
  Authors
Vrielink A
  Title
Cholesterol oxidase: structure and function.
  Journal
Subcell Biochem 51:137-58 (2010)
DOI:10.1007/978-90-481-8622-8_5
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.6
IUBMB Enzyme Nomenclature: 1.1.3.6
ExPASy - ENZYME nomenclature database: 1.1.3.6
BRENDA, the Enzyme Database: 1.1.3.6
CAS: 9028-76-6

KEGG   ENZYME: 5.3.3.1Help
Entry
EC 5.3.3.1                  Enzyme                                 

Name
steroid Delta-isomerase;
hydroxysteroid isomerase;
steroid isomerase;
Delta5-ketosteroid isomerase;
Delta5(or Delta4)-3-keto steroid isomerase;
Delta5-steroid isomerase;
3-oxosteroid isomerase;
Delta5-3-keto steroid isomerase;
Delta5-3-oxosteroid isomerase
Class
Isomerases;
Intramolecular oxidoreductases;
Transposing C=C bonds
BRITE hierarchy
Sysname
3-oxosteroid Delta5-Delta4-isomerase
Reaction(IUBMB)
a 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid [RN:R02644]
Reaction(KEGG)
Substrate
3-oxo-Delta5-steroid [CPD:C01034]
Product
3-oxo-Delta4-steroid [CPD:C00619]
Comment
This activity is catalysed by several distinct enzymes (cf. EC 1.1.3.6, cholesterol oxidase and EC 1.1.1.145, 3-hydroxy-5-steroid dehydrogenase).
History
EC 5.3.3.1 created 1961
Pathway
ec00140  Steroid hormone biosynthesis
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K01822  steroid Delta-isomerase
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA: 3283(HSD3B1) 3284(HSD3B2)
PTR: 457165 469446
PPS: 100982464(HSD3B2) 100983009 100990187
GGO: 101141070 101141801 101146563(HSD3B2)
PON: 100451655 100452394
NLE: 100596087 100605995(HSD3B2) 100606563 100607955
MCC: 712686(HSD3B2) 713091
MCF: 102129497 102133592(HSD3B1) 102134747 102135110
CSAB: 103224104 103224109(HSD3B2)
RRO: 104654599 104654602 104654605
MMU: 100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
HGL: 101707488(Hsd3b1) 101707858
TUP: 102470981
CFA: 483146(HSD3B2)
VVP: 112916789
AML: 100464280(HSD3B1)
UMR: 103677123
UAH: 113244362
ORO: 101378904
ELK: 111143436
FCA: 100616070(HSD3B2)
PTG: 102957262
PPAD: 109272160
AJU: 106978496
BTA: 281824(HSD3B1)
BOM: 102274088
BIU: 109555859
BBUB: 102413429
CHX: 100860869(3BHSD)
OAS: 100190978(HSD3B1)
SSC: 445539(HSD3B1)
CFR: 102512447
CDK: 105089475
BACU: 103015346
LVE: 103083046
OOR: 101284283
DLE: 111179902
PCAD: 102987751
ECB: 100034078(HSD3B2)
EPZ: 103548379
EAI: 106832920(HSD3B2)
MYB: 106723550(HSD3B2)
MNA: 107545288(HSD3B2)
HAI: 109389868(HSD3B2)
DRO: 112314004(HSD3B2)
PALE: 102891879
RAY: 107518830(HSD3B2)
MJV: 108390800
LAV: 100659049(HSD3B2)
TMU: 101358325
MDO: 100012668
SHR: 100913530(HSD3B2)
PCW: 110219672(HSD3B2)
OAA: 100080135
GGA: 396015(HSD3B1)
MGP: 104915630
CJO: 107319373(3beta-HSD)
NMEL: 110398845
APLA: 101803865
ACYG: 106045741
TGU: 751770(HSD3B1)
LSR: 110480744
SCAN: 103827136
GFR: 102039409
FAB: 101817697
PHI: 102101526
PMAJ: 107211459
CCAE: 111938581
CCW: 104687106
ETL: 114058198
FPG: 101921564
FCH: 102052697
CLV: 102087460
EGZ: 104126518
NNI: 104008615
ACUN: 113485872
PADL: 103921223
AAM: 106497227
ASN: 102367786(HSD3B1)
AMJ: 109280168(HSD3B1)
PSS: 102453952
CMY: 102934871
CPIC: 101946936
ACS: 100561899(hsd3b1)
PVT: 110076926
PBI: 103067418
PMUR: 107285816
TSR: 106542700
PMUA: 114587929(HSD3B2)
GJA: 107114856
XLA: 734818(hsd3b1.L)
XTR: 100490622
NPR: 108802532
DRE: 373131(hsd3b2) 553532(hsd3b1)
SRX: 107712267
SANH: 107658243
SGH: 107569293
CCAR: 109052439
IPU: 100304475(HSD3B)
PHYP: 113524942(hsd3b1)
AMEX: 103027852(hsd3b1)
EEE: 113587753
TRU: 101069884(hsd3b1)
LCO: 104921488(hsd3b1)
MZE: 101485719
ONL: 100534568
OLA: 100144358(3bhsd)
XMA: 102228303
XCO: 114147930
PRET: 103477979
CVG: 107087352
NFU: 107389612(hsd3b1)
KMR: 108244192(hsd3b1)
ALIM: 106531920
AOCE: 111570570(hsd3b1)
CSEM: 103391994(hsd3b1)
POV: 109637945
LCF: 108902437
SDU: 111218347
SLAL: 111652102
HCQ: 109522071
BPEC: 110156192(hsd3b1)
MALB: 109951646
SASA: 106586600
OTW: 112240651
SALP: 111978723
ELS: 105016430
SFM: 108937872
PKI: 111860692
LCM: 102345588
CMK: 103180179
RTP: 109918392
DME: Dmel_CG7724(CG7724)
DER: 6543694
DSI: Dsimw501_GD14693(Dsim_GD14693)
DPE: 6603899
DMN: 108165559
DWI: 6645028
DAZ: 108612818
DNV: 108651018
DHE: 111602930
MDE: 101899660
LCQ: 111691019
AAG: 5566270
NVI: 100120136
CSOL: 105366624
CLEC: 106661338
ZNE: 110831195
FCD: 110862702
PVM: 113815316
TUT: 107363285
DPTE: 113794090
CSCU: 111622487
PTEP: 107437550
LAK: 106151343
PRE: PCA10_13780(ksi)
PKC: PKB_4244
PSET: THL1_1429
SPL: Spea_2174
SHL: Shal_2145
GPB: HDN1F_02730(ksi)
REH: H16_B0602(h16_B0602)
BFN: OI25_4583
AJS: Ajs_2963
CTES: O987_07595
AZO: azo3316
XAU: Xaut_1492
HNE: HNE_1461
SWI: Swit_3295
SPHD: HY78_09075
SSAN: NX02_14810
SFLA: SPHFLASMR4Y_01939(ksi)
OIH: OB0288
MLE: ML1942
MLB: MLBr01942
MAB: MAB_1206
MABB: MASS_1204
MCHE: BB28_05990
MSTE: MSTE_01173
MSAL: DSM43276_01073(ksi)
ASD: AS9A_0954
NFR: ERS450000_01854(ksi_1) ERS450000_02770(ksi_2)
RER: RER_13740(ksi) RER_59940
ROP: ROP_34080(ksi)
RRT: 4535765_04040(ksi_1) 4535765_04891(ksi_2)
GBR: Gbro_4817
GOR: KTR9_4702
GRU: GCWB2_07285(ksi1) GCWB2_23650(ksi2)
GOM: D7316_02575(ksi_1) D7316_03579(ksi_2)
SGR: SGR_6072
SBH: SBI_01379
NCA: Noca_3954
NDK: I601_0503(ksi)
FAL: FRAAL4373
SEN: SACE_4441
AMYY: YIM_36650(ksi)
SAQ: Sare_2441
 » show all
Taxonomy
Reference
1  [PMID:5867327]
  Authors
Ewald W, Werbin H, Chaikoff IL.
  Title
Evidence for the presence of 17-hydroxypregnenedione isomerase in beef adrenal cortex.
  Journal
Biochim Biophys Acta 111:306-12 (1965)
DOI:10.1016/0304-4165(65)90497-6
Reference
2  [PMID:14404954]
  Authors
KAWAHARA FS, TALALAY P.
  Title
Crystalline Delta 5-3-ketosteroid isomerase.
  Journal
J Biol Chem 235:PC1-2 (1960)
Reference
3  [PMID:13276386]
  Authors
TALALAY P, WANG VS.
  Title
Enzymic isomerization of delta5-3-ketosteroids.
  Journal
Biochim Biophys Acta 18:300-1 (1955)
Reference
4  [PMID:10822008]
  Authors
MacLachlan J, Wotherspoon AT, Ansell RO, Brooks CJ
  Title
Cholesterol oxidase: sources, physical properties and analytical applications.
  Journal
J Steroid Biochem Mol Biol 72:169-95 (2000)
DOI:10.1016/S0960-0760(00)00044-3
Other DBs
ExplorEnz - The Enzyme Database: 5.3.3.1
IUBMB Enzyme Nomenclature: 5.3.3.1
ExPASy - ENZYME nomenclature database: 5.3.3.1
BRENDA, the Enzyme Database: 5.3.3.1
CAS: 9031-36-1

KEGG   REACTION: R01459Help
Entry
R01459                      Reaction                               

Name
Cholesterol:oxygen oxidoreductase
Definition
Cholesterol + Oxygen <=> Cholest-4-en-3-one + Hydrogen peroxide
Equation
Reaction class
RC00146  C00187_C00599
RC02755  C00007_C00027
Enzyme
Pathway
rn00984  Steroid degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K03333  cholesterol oxidase [EC:1.1.3.6]
Other DBs
RHEA: 32186

KEGG   REACTION: R09920Help
Entry
R09920                      Reaction                               

Name
cholesterol:NAD+ 3-oxidoreductase;
cholest-4-en-3-one delta5-delat4-isomerase
Definition
Cholesterol + NAD+ <=> Cholest-4-en-3-one + NADH + H+
Equation
Comment
multi-step reaction (see R03176+R02644)
Reaction class
RC00001  C00003_C00004
RC00146  C00187_C00599
Enzyme
1.1.1.145       5.3.3.1
Pathway
rn00984  Steroid degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]

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