KEGG   ORTHOLOGY: K03396
Entry
K03396                      KO                                     

Name
gfa
Definition
S-(hydroxymethyl)glutathione synthase [EC:4.4.1.22]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K03396  gfa; S-(hydroxymethyl)glutathione synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.4  Carbon-sulfur lyases
   4.4.1  Carbon-sulfur lyases (only sub-subclass identified to date)
    4.4.1.22  S-(hydroxymethyl)glutathione synthase
     K03396  gfa; S-(hydroxymethyl)glutathione synthase
Other DBs
RN: R06982
GO: 0051907
Genes
SMP: SMAC_08712
PAN: PODANSg505
TTT: THITE_49799
TMN: UCRPA7_692
SSCK: SPSK_01984
FGR: FGSG_10776
FPU: FPSE_08822
FVR: FVEG_11444
FOX: FOXG_12705
NHE: NECHADRAFT_69107
MAJ: MAA_09901
ANG: ANI_1_14024(An02g00080)
PTE: PTT_17259
ADP: NCTC12871_00268(gfa)
XCC: XCC3388
XCB: XC_0776
XCA: xcc-b100_0811(gfa)
XCP: XCR_3724(gfa)
XCV: XCV0789
XAX: XACM_0733
XAC: XAC0735
XOM: XOO3646(XOO3646)
XOP: PXO_04414
XPH: XppCFBP6546_16605(XppCFBP6546P_16605)
XVA: C7V42_18175(gfa)
XHY: FZ025_12510(gfa)
XTH: G4Q83_19135(gfa)
DKO: I596_2122
VFI: VF_A0007
VFM: VFMJ11_A0021(gfa)
VSA: VSAL_II0213(gfa)
PPR: PBPRB1570
PCQ: PcP3B5_24880(gfa)
PPX: T1E_5519
PSB: Psyr_2957
PPUU: PputUW4_02203(gfa)
PKC: PKB_4293(gfa)
PSIL: PMA3_14295
ATN: FM020_06240(gfa)
SSE: Ssed_4230
SWD: Swoo_0373
SVO: SVI_3944(gfa)
MVS: MVIS_3174(gfa)
MMAA: FR932_02530(gfa)
SALN: SALB1_1489
BAM: Bamb_5797
AMIM: MIM_c14300(gfa)
VEI: Veis_4024
MMS: mma_2819
MALI: EYF70_29695(gfa)
MUM: FCL38_19970(gfa)
MFLA: GO485_25000(gfa)
MPLI: E1742_23860(gfa)
URU: DSM104443_01527(gfa)
MLO: mlr0874
MHUA: MCHK_2549(gfa)
MJR: EB229_21270(gfa)
MERD: EB233_18455(gfa)
AMIH: CO731_01953(gfa_1) CO731_02450(gfa_2)
SME: SM_b20186
SMI: BN406_06470(gfa)
SMEL: SM2011_b20186(gfa)
SMER: DU99_32400
SMD: Smed_3930
RHI: NGR_b14630(gfa1) NGR_c19300 NGR_c20490(gfa2)
SAME: SAMCFNEI73_Ch0088(gfa)
EAD: OV14_a1033(gfa2)
EMX: FKV68_00780(gfa) FKV68_13155(gfa) FKV68_33100(gfa)
REL: REMIM1_PF00508(gfa)
REP: IE4803_PC00392(gfa)
REI: IE4771_PD00410(gfa)
RLE: pRL120526(gfA)
RLG: Rleg_4832
RHL: LPU83_2360(gfa)
RGA: RGR602_PC00929(gfa-1) RGR602_PC02286(gfa-2)
RHN: AMJ98_PE00477(gfa)
RPHA: AMC79_PD00607(gfa)
RHX: AMK02_PD00340(gfa)
RHK: Kim5_PC00370(gfa)
REZ: AMJ99_PD00477(gfa)
RJG: CCGE525_10885(gfa)
RGR: FZ934_22410(gfa)
RAD: CO657_27365(gfa)
RHT: NT26_3258(gfa)
SHZ: shn_31780
KAI: K32_22190(gfa)
BJA: blr6216(blr6216)
BRA: BRADO5487(gfa)
BBT: BBta_5971(gfa)
BRS: S23_20790
AOL: S58_22210
BRO: BRAD285_1746(gfa)
BRK: CWS35_16195(gfa) CWS35_32580(gfa)
BOT: CIT37_23150(gfa)
BRQ: CIT40_23255(gfa)
BGQ: X265_11175(gfa)
BGZ: XH91_10400(gfa)
BSYM: CIT39_22250(gfa)
BBET: F8237_25275(gfa)
BARH: WN72_34695(gfa)
BVZ: BRAD3257_2931(gfa)
RPC: RPC_0100
RPE: RPE_4679
TRB: HB776_18780(gfa)
BRN: D1F64_20380(gfa)
NOH: G5V57_12155(gfa)
RSP: RSP_2575
PDE: Pden_0015
PYE: A6J80_09895(gfa)
PZH: CX676_13675(gfa)
PARO: CUV01_07510(gfa)
PAMN: JCM7685_0202(gfa)
PMUT: DPM13_08095(gfa)
PARS: DRW48_11880(gfa)
PARR: EOJ32_02150(gfa)
PKD: F8A10_00210(gfa)
PPAN: ESD82_21255(gfa)
YPAC: CEW88_21860(gfa)
SAGU: CDO87_25880(gfa)
HML: HmaOT1_09240(gfa)
OCD: FHY55_11710(gfa)
PPRU: FDP22_18560(gfa)
MALU: KU6B_56540(gfa)
PAMO: BAR1_01170(gfa)
SPHA: D3Y57_02580(gfa)
ACR: Acry_2793
AMV: ACMV_31250(gfa)
SWF: E3E12_02505(gfa)
AZZ: DEW08_23520(gfa)
MAGQ: MGMAQ_0499(gfa)
HTQ: FRZ44_12310(gfa)
HADH: FRZ61_12390(gfa)
AFR: AFE_0696(gfa)
SIV: SSIL_2807
CRN: CAR_c23670(gfa)
 » show all
Reference
  Authors
Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA
  Title
A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy.
  Journal
J Biol Chem 277:3069-72 (2002)
DOI:10.1074/jbc.C100579200
  Sequence
[pde:Pden_0015]

KEGG   ENZYME: 4.4.1.22
Entry
EC 4.4.1.22                 Enzyme                                 

Name
S-(hydroxymethyl)glutathione synthase;
glutathione-dependent formaldehyde-activating enzyme;
Gfa;
S-(hydroxymethyl)glutathione formaldehyde-lyase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
S-(hydroxymethyl)glutathione formaldehyde-lyase (glutathione-forming)
Reaction(IUBMB)
S-(hydroxymethyl)glutathione = glutathione + formaldehyde [RN:R06982]
Reaction(KEGG)
R06982
Substrate
S-(hydroxymethyl)glutathione [CPD:C14180]
Product
glutathione [CPD:C00051];
formaldehyde [CPD:C00067]
Comment
The enzyme from Paracoccus denitrificans accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway.
History
EC 4.4.1.22 created 2005 (EC 1.2.1.1 created 1961, modified 1982, modified 2002, part transferred 2005 to EC 4.4.1.22)
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03396  S-(hydroxymethyl)glutathione synthase
Genes
SMP: SMAC_08712
PAN: PODANSg505
TTT: THITE_49799
TMN: UCRPA7_692
SSCK: SPSK_01984
FGR: FGSG_10776
FPU: FPSE_08822
FVR: FVEG_11444
FOX: FOXG_12705
NHE: NECHADRAFT_69107
MAJ: MAA_09901
ANG: ANI_1_14024(An02g00080)
PTE: PTT_17259
ADP: NCTC12871_00268(gfa)
XCC: XCC3388
XCB: XC_0776
XCA: xcc-b100_0811(gfa)
XCP: XCR_3724(gfa)
XCV: XCV0789
XAX: XACM_0733
XAC: XAC0735
XOM: XOO3646(XOO3646)
XOP: PXO_04414
XPH: XppCFBP6546_16605(XppCFBP6546P_16605)
XVA: C7V42_18175(gfa)
XHY: FZ025_12510(gfa)
XTH: G4Q83_19135(gfa)
DKO: I596_2122
VFI: VF_A0007
VFM: VFMJ11_A0021(gfa)
VSA: VSAL_II0213(gfa)
PPR: PBPRB1570
PCQ: PcP3B5_24880(gfa)
PPX: T1E_5519
PSB: Psyr_2957
PPUU: PputUW4_02203(gfa)
PKC: PKB_4293(gfa)
PSIL: PMA3_14295
ATN: FM020_06240(gfa)
SSE: Ssed_4230
SWD: Swoo_0373
SVO: SVI_3944(gfa)
MVS: MVIS_3174(gfa)
MMAA: FR932_02530(gfa)
SALN: SALB1_1489
BAM: Bamb_5797
AMIM: MIM_c14300(gfa)
VEI: Veis_4024
MMS: mma_2819
MALI: EYF70_29695(gfa)
MUM: FCL38_19970(gfa)
MFLA: GO485_25000(gfa)
MPLI: E1742_23860(gfa)
URU: DSM104443_01527(gfa)
MLO: mlr0874
MHUA: MCHK_2549(gfa)
MJR: EB229_21270(gfa)
MERD: EB233_18455(gfa)
AMIH: CO731_01953(gfa_1) CO731_02450(gfa_2)
SME: SM_b20186
SMI: BN406_06470(gfa)
SMEL: SM2011_b20186(gfa)
SMER: DU99_32400
SMD: Smed_3930
RHI: NGR_b14630(gfa1) NGR_c19300 NGR_c20490(gfa2)
SAME: SAMCFNEI73_Ch0088(gfa)
EAD: OV14_a1033(gfa2)
EMX: FKV68_00780(gfa) FKV68_13155(gfa) FKV68_33100(gfa)
REL: REMIM1_PF00508(gfa)
REP: IE4803_PC00392(gfa)
REI: IE4771_PD00410(gfa)
RLE: pRL120526(gfA)
RLG: Rleg_4832
RHL: LPU83_2360(gfa)
RGA: RGR602_PC00929(gfa-1) RGR602_PC02286(gfa-2)
RHN: AMJ98_PE00477(gfa)
RPHA: AMC79_PD00607(gfa)
RHX: AMK02_PD00340(gfa)
RHK: Kim5_PC00370(gfa)
REZ: AMJ99_PD00477(gfa)
RJG: CCGE525_10885(gfa)
RGR: FZ934_22410(gfa)
RAD: CO657_27365(gfa)
RHT: NT26_3258(gfa)
SHZ: shn_31780
KAI: K32_22190(gfa)
BJA: blr6216(blr6216)
BRA: BRADO5487(gfa)
BBT: BBta_5971(gfa)
BRS: S23_20790
AOL: S58_22210
BRO: BRAD285_1746(gfa)
BRK: CWS35_16195(gfa) CWS35_32580(gfa)
BOT: CIT37_23150(gfa)
BRQ: CIT40_23255(gfa)
BGQ: X265_11175(gfa)
BGZ: XH91_10400(gfa)
BSYM: CIT39_22250(gfa)
BBET: F8237_25275(gfa)
BARH: WN72_34695(gfa)
BVZ: BRAD3257_2931(gfa)
RPC: RPC_0100
RPE: RPE_4679
TRB: HB776_18780(gfa)
BRN: D1F64_20380(gfa)
NOH: G5V57_12155(gfa)
RSP: RSP_2575
PDE: Pden_0015
PYE: A6J80_09895(gfa)
PZH: CX676_13675(gfa)
PARO: CUV01_07510(gfa)
PAMN: JCM7685_0202(gfa)
PMUT: DPM13_08095(gfa)
PARS: DRW48_11880(gfa)
PARR: EOJ32_02150(gfa)
PKD: F8A10_00210(gfa)
PPAN: ESD82_21255(gfa)
YPAC: CEW88_21860(gfa)
SAGU: CDO87_25880(gfa)
HML: HmaOT1_09240(gfa)
OCD: FHY55_11710(gfa)
PPRU: FDP22_18560(gfa)
MALU: KU6B_56540(gfa)
PAMO: BAR1_01170(gfa)
SPHA: D3Y57_02580(gfa)
ACR: Acry_2793
AMV: ACMV_31250(gfa)
SWF: E3E12_02505(gfa)
AZZ: DEW08_23520(gfa)
MAGQ: MGMAQ_0499(gfa)
HTQ: FRZ44_12310(gfa)
HADH: FRZ61_12390(gfa)
AFR: AFE_0696(gfa)
SIV: SSIL_2807
CRN: CAR_c23670(gfa)
 » show all
Reference
1  [PMID:11741920]
  Authors
Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA
  Title
A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy.
  Journal
J Biol Chem 277:3069-72 (2002)
DOI:10.1074/jbc.C100579200
  Sequence
[pde:Pden_0015]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.22
IUBMB Enzyme Nomenclature: 4.4.1.22
ExPASy - ENZYME nomenclature database: 4.4.1.22
UM-BBD (Biocatalysis/Biodegradation Database): 4.4.1.22
BRENDA, the Enzyme Database: 4.4.1.22
CAS: 425642-27-9

KEGG   REACTION: R06982
Entry
R06982                      Reaction                               

Name
S-(hydroxymethyl)glutathione formaldehyde-lyase (glutathione-forming);
S-(hydroxymethyl)glutathione synthase
Definition
Glutathione + Formaldehyde <=> S-(Hydroxymethyl)glutathione
Equation
Comment
spontaneous
This reaction is accelerated by enzyme ec 4.4.1.22.
Reaction class
RC00069  C00051_C14180
RC01707  C00067_C14180
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Orthology
K03396  S-(hydroxymethyl)glutathione synthase [EC:4.4.1.22]
Other DBs
RHEA: 22491

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