KEGG   ORTHOLOGY: K04480
Entry
K04480                      KO                                     

Name
mtaB
Definition
methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase [EC:2.1.1.90]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00356  Methanogenesis, methanol => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K04480  mtaB; methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.90  methanol---corrinoid protein Co-methyltransferase
     K04480  mtaB; methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase
Other DBs
RN: R04384 R09098
GO: 0047152
Genes
DSX: GD604_09795
DTI: Desti_2034
CFM: BJL90_02615
CCL: Clocl_0783
ANR: Ana3638_17590
DKU: Desku_0051
TJR: TherJR_0987
DOR: Desor_2803
DMI: Desmer_2205
ELM: ELI_2003
AWO: Awo_c22760(mttB18)
MTA: Moth_1209
TPZ: Tph_c03590(mtaB)
MST: Msp_0184(mtaB3) Msp_0186(mtaB2) Msp_0188(mtaB1) Msp_0819(mtaB4)
MSI: Msm_0515
MCUB: MCBB_1154(mtaB)
MAC: MA_0455(mtaB1) MA_1616(mtaB3) MA_4392(mtaB2)
MMA: MM_0175(mtaB) MM_1074(mtaB) MM_1647
RCI: RCIX1974(mtaB)
MEAR: Mpt1_c05090(mtaB)
 » show all
Reference
  Authors
Pritchett MA, Metcalf WW
  Title
Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.
  Journal
Mol Microbiol 56:1183-94 (2005)
DOI:10.1111/j.1365-2958.2005.04616.x
  Sequence

KEGG   ENZYME: 2.1.1.90
Entry
EC 2.1.1.90                 Enzyme                                 

Name
methanol---corrinoid protein Co-methyltransferase;
methanol cobalamin methyltransferase;
methanol:5-hydroxybenzimidazolylcobamide methyltransferase;
MT 1 (ambiguous);
methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase;
mtaB (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methanol:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
methanol + a [Co(I) methanol-specific corrinoid protein] = a [methyl-Co(III) methanol-specific corrinoid protein] + H2O [RN:R04384]
Reaction(KEGG)
R04384;
(other) R09098
Substrate
methanol [CPD:C00132];
[Co(I) methanol-specific corrinoid protein]
Product
[methyl-Co(III) methanol-specific corrinoid protein];
H2O [CPD:C00001]
Comment
The enzyme, which catalyses the transfer of methyl groups from methanol to a methanol-specific corrinoid protein (MtaC), is involved in methanogenesis from methanol. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. Free cob(I)alamin can substitute for the corrinoid protein in vitro [2].
Inactivated by oxygen and other oxidizing agents, and reactivated by catalytic amounts of ATP and hydrogen.
History
EC 2.1.1.90 created 1989, modified 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K04480  methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase
Genes
DSX: GD604_09795
DTI: Desti_2034
CFM: BJL90_02615
CCL: Clocl_0783
ANR: Ana3638_17590
DKU: Desku_0051
TJR: TherJR_0987
DOR: Desor_2803
DMI: Desmer_2205
ELM: ELI_2003
AWO: Awo_c22760(mttB18)
MTA: Moth_1209
TPZ: Tph_c03590(mtaB)
MST: Msp_0184(mtaB3) Msp_0186(mtaB2) Msp_0188(mtaB1) Msp_0819(mtaB4)
MSI: Msm_0515
MCUB: MCBB_1154(mtaB)
MAC: MA_0455(mtaB1) MA_1616(mtaB3) MA_4392(mtaB2)
MMA: MM_0175(mtaB) MM_1074(mtaB) MM_1647
RCI: RCIX1974(mtaB)
MEAR: Mpt1_c05090(mtaB)
 » show all
Reference
1  [PMID:6438059]
  Authors
van der Meijden P, te Brommelstroet BW, Poirot CM, van der Drift C, Vogels GD.
  Title
Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri.
  Journal
J Bacteriol 160:629-35 (1984)
DOI:10.1128/JB.160.2.629-635.1984
  Sequence
Reference
2  [PMID:10215883]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin.
  Journal
Eur J Biochem 261:674-81 (1999)
DOI:10.1046/j.1432-1327.1999.00355.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.90
IUBMB Enzyme Nomenclature: 2.1.1.90
ExPASy - ENZYME nomenclature database: 2.1.1.90
BRENDA, the Enzyme Database: 2.1.1.90
CAS: 86611-98-5

KEGG   REACTION: R09098
Entry
R09098                      Reaction                               

Name
methanol:coenzyme M methyltransferase
Definition
Methanol + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + H2O
Equation
Comment
two-step reaction (see R04384+R10000)
Reaction class
RC00035  C03576_C03920
RC02440  C00132_C03920
Enzyme
2.1.1.90        2.1.1.246
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00356  Methanogenesis, methanol => methane
Orthology
K04480  methanol---5-hydroxybenzimidazolylcobamide Co-methyltransferase [EC:2.1.1.90]
K14080  [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.246]
K14081  methanol corrinoid protein

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