KEGG   ORTHOLOGY: K05597
Entry
K05597                      KO                                     

Name
aspQ, ansB, ansA
Definition
glutamin-(asparagin-)ase [EC:3.5.1.38]
Pathway
ko00220  Arginine biosynthesis
ko00250  Alanine, aspartate and glutamate metabolism
ko00471  D-Glutamine and D-glutamate metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko02020  Two-component system
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00250 Alanine, aspartate and glutamate metabolism
    K05597  aspQ, ansB, ansA; glutamin-(asparagin-)ase
   00220 Arginine biosynthesis
    K05597  aspQ, ansB, ansA; glutamin-(asparagin-)ase
  09106 Metabolism of other amino acids
   00471 D-Glutamine and D-glutamate metabolism
    K05597  aspQ, ansB, ansA; glutamin-(asparagin-)ase
 09130 Environmental Information Processing
  09132 Signal transduction
   02020 Two-component system
    K05597  aspQ, ansB, ansA; glutamin-(asparagin-)ase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.38  glutamin-(asparagin-)ase
     K05597  aspQ, ansB, ansA; glutamin-(asparagin-)ase
Other DBs
RN: R00256 R00485 R01579
COG: COG0252
GO: 0050417
Genes
LVE: 103069015 103085390 103091282
PAE: PA1337(ansB)
PAEV: N297_1377(ansB)
PAEI: N296_1377(ansB)
PAU: PA14_46970(ansB)
PAP: PSPA7_4048(ansB)
PAG: PLES_38441(ansB)
PAF: PAM18_3565(ansB)
PNC: NCGM2_2225(ansB)
PAEB: NCGM1900_5234(ansB)
PAEP: PA1S_19220
PAEM: U769_19065
PAEL: T223_19670
PAEU: BN889_01444(ansB)
PAEG: AI22_15380
PAEC: M802_1374(ansB)
PAEO: M801_1376(ansB)
PPU: PP_2453(ansB)
PPF: Pput_3237
PPT: PPS_2020
PPI: YSA_00932
PPX: T1E_3434(ansA)
PPUH: B479_09975
PPUT: L483_21245
PPUN: PP4_19030(ansB)
PPUD: DW66_2273
PMON: X969_08385
PMOT: X970_08045
PFL: PFL_2099(ansB)
PPRC: PFLCHA0_c21450(ansB)
PPRO: PPC_2112(ansB)
PFS: PFLU_4161
PFC: PflA506_3470(ansB)
PFB: VO64_1240
PMAN: OU5_5665(ansB)
PFW: PF1751_v1c36680(ansB)
PEN: PSEEN1951(aspQ)
PPUU: PputUW4_03404(ansB)
PSEM: TO66_10680
PSEC: CCOS191_2092(ansB)
PSOS: POS17_2054(ansB)
PANR: A7J50_3841
PSIL: PMA3_09280
ACB: A1S_1466
ABM: ABSDF2183(aspQ)
ABY: ABAYE2188(aspQ)
ABN: AB57_1697
ABB: ABBFA_01996(ansB)
ABX: ABK1_1959
ABH: M3Q_1853
ABAD: ABD1_14640(aspQ)
ABAZ: P795_9890
ABAU: IX87_02900
ABAA: IX88_09280
ACC: BDGL_000847(aspQ)
ACI: ACIAD2088(aspQ)
AGU: AS4_18910(aspQ)
AUG: URS_2403
REH: H16_A1910(ansA)
CNC: CNE_1c18890(ansB2)
RME: Rmet_1583(ansB)
CTI: RALTA_A1603(ansB1)
CGD: CR3_4545(aspQ)
BMUL: NP80_1014(ansB)
BGO: BM43_2101(ansB)
BGP: BGL_1c06540(ansB1)
BXE: Bxe_B2492
BXB: DR64_4819
HYF: DTO96_101665(ansB_2)
POL: Bpro_4206
PNA: Pnap_0442
AAV: Aave_4182
AJS: Ajs_3863
AAA: Acav_4052
ACRA: BSY15_2021(ansB)
DAC: Daci_0564
VPD: VAPA_1c49020(ansB)
CTES: O987_02085
MPT: Mpe_A1913
HSE: Hsero_1444(ansB)
LCH: Lcho_2689
PBH: AAW51_1003(aspQ)
AZO: azo0438(ansB2)
AZA: AZKH_3046(ansB2)
AOA: dqs_0449
DTR: RSDT_0924(ansB)
MET: M446_5042
MNO: Mnod_5349
MOR: MOC_3938
MAQU: Maq22A_c08785(ansB)
 » show all

KEGG   ENZYME: 3.5.1.38
Entry
EC 3.5.1.38                 Enzyme                                 

Name
glutamin-(asparagin-)ase;
glutaminase-asparaginase;
ansB (gene name);
L-asparagine/L-glutamine amidohydrolase;
L-ASNase/L-GLNase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
L-glutamine(L-asparagine) amidohydrolase
Reaction(IUBMB)
(1) L-glutamine + H2O = L-glutamate + NH3 [RN:R00256];
(2) L-asparagine + H2O = L-aspartate + NH3 [RN:R00485]
Reaction(KEGG)
R00256 R00485;
(other) R01579 R06134
Substrate
L-glutamine [CPD:C00064];
H2O [CPD:C00001];
L-asparagine [CPD:C00152]
Product
L-glutamate [CPD:C00025];
NH3 [CPD:C00014];
L-aspartate [CPD:C00049]
Comment
The enzyme from the bacterium Achromobacter hydrolyses L-asparagine at 0.8 of the rate of L-glutamine; the D-isomers are also hydrolysed, but more slowly. cf. EC 3.5.1.2, glutaminase and EC 3.5.1.1, asparaginase.
History
EC 3.5.1.38 created 1976
Pathway
ec00220  Arginine biosynthesis
ec00250  Alanine, aspartate and glutamate metabolism
ec00471  D-Glutamine and D-glutamate metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05597  glutamin-(asparagin-)ase
Genes
LVE: 103069015 103085390 103091282
PAE: PA1337(ansB)
PAEV: N297_1377(ansB)
PAEI: N296_1377(ansB)
PAU: PA14_46970(ansB)
PAP: PSPA7_4048(ansB)
PAG: PLES_38441(ansB)
PAF: PAM18_3565(ansB)
PNC: NCGM2_2225(ansB)
PAEB: NCGM1900_5234(ansB)
PAEP: PA1S_19220
PAEM: U769_19065
PAEL: T223_19670
PAEU: BN889_01444(ansB)
PAEG: AI22_15380
PAEC: M802_1374(ansB)
PAEO: M801_1376(ansB)
PPU: PP_2453(ansB)
PPF: Pput_3237
PPT: PPS_2020
PPI: YSA_00932
PPX: T1E_3434(ansA)
PPUH: B479_09975
PPUT: L483_21245
PPUN: PP4_19030(ansB)
PPUD: DW66_2273
PMON: X969_08385
PMOT: X970_08045
PFL: PFL_2099(ansB)
PPRC: PFLCHA0_c21450(ansB)
PPRO: PPC_2112(ansB)
PFS: PFLU_4161
PFC: PflA506_3470(ansB)
PFB: VO64_1240
PMAN: OU5_5665(ansB)
PFW: PF1751_v1c36680(ansB)
PEN: PSEEN1951(aspQ)
PPUU: PputUW4_03404(ansB)
PSEM: TO66_10680
PSEC: CCOS191_2092(ansB)
PSOS: POS17_2054(ansB)
PANR: A7J50_3841
PSIL: PMA3_09280
ACB: A1S_1466
ABM: ABSDF2183(aspQ)
ABY: ABAYE2188(aspQ)
ABN: AB57_1697
ABB: ABBFA_01996(ansB)
ABX: ABK1_1959
ABH: M3Q_1853
ABAD: ABD1_14640(aspQ)
ABAZ: P795_9890
ABAU: IX87_02900
ABAA: IX88_09280
ACC: BDGL_000847(aspQ)
ACI: ACIAD2088(aspQ)
AGU: AS4_18910(aspQ)
AUG: URS_2403
REH: H16_A1910(ansA)
CNC: CNE_1c18890(ansB2)
RME: Rmet_1583(ansB)
CTI: RALTA_A1603(ansB1)
CGD: CR3_4545(aspQ)
BMUL: NP80_1014(ansB)
BGO: BM43_2101(ansB)
BGP: BGL_1c06540(ansB1)
BXE: Bxe_B2492
BXB: DR64_4819
HYF: DTO96_101665(ansB_2)
POL: Bpro_4206
PNA: Pnap_0442
AAV: Aave_4182
AJS: Ajs_3863
AAA: Acav_4052
ACRA: BSY15_2021(ansB)
DAC: Daci_0564
VPD: VAPA_1c49020(ansB)
CTES: O987_02085
MPT: Mpe_A1913
HSE: Hsero_1444(ansB)
LCH: Lcho_2689
PBH: AAW51_1003(aspQ)
AZO: azo0438(ansB2)
AZA: AZKH_3046(ansB2)
AOA: dqs_0449
DTR: RSDT_0924(ansB)
MET: M446_5042
MNO: Mnod_5349
MOR: MOC_3938
MAQU: Maq22A_c08785(ansB)
 » show all
Reference
1  [PMID:5017769]
  Authors
Roberts J, Holcenberg JS, Dolowy WC.
  Title
Isolation, crystallization, and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity.
  Journal
J Biol Chem 247:84-90 (1972)
Reference
2  [PMID:3379033]
  Authors
Tanaka S, Robinson EA, Appella E, Miller M, Ammon HL, Roberts J, Weber IT, Wlodawer A
  Title
Structures of amidohydrolases. Amino acid sequence of a glutaminase-asparaginase  from Acinetobacter glutaminasificans and preliminary crystallographic data for an asparaginase from Erwinia chrysanthemi.
  Journal
J Biol Chem 263:8583-91 (1988)
Reference
3  [PMID:8068664]
  Authors
Lubkowski J, Wlodawer A, Ammon HL, Copeland TD, Swain AL
  Title
Structural characterization of Pseudomonas 7A glutaminase-asparaginase.
  Journal
Biochemistry 33:10257-65 (1994)
DOI:10.1021/bi00200a005
  Sequence
Reference
4  [PMID:10684596]
  Authors
Ortlund E, Lacount MW, Lewinski K, Lebioda L
  Title
Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu.
  Journal
Biochemistry 39:1199-204 (2000)
DOI:10.1021/bi991797d
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.38
IUBMB Enzyme Nomenclature: 3.5.1.38
ExPASy - ENZYME nomenclature database: 3.5.1.38
BRENDA, the Enzyme Database: 3.5.1.38
CAS: 39335-03-0

KEGG   REACTION: R00256
Entry
R00256                      Reaction                               

Name
L-glutamine amidohydrolase
Definition
L-Glutamine + H2O <=> L-Glutamate + Ammonia
Equation
Comment
the first step of several ligases (ec 6.3.5.x) using glutamine as amide-N-donor
ec 6.3.5.4 (see R00578, R00256+R00483)
ec 6.3.5.5 (see R00575, R00256+R10948+R10949+R01395)
Reaction class
RC00010  C00025_C00064
RC02798  C00014_C00064
Enzyme
1.4.1.13        1.4.1.14        1.4.7.1         3.5.1.2         
3.5.1.38        4.3.2.10        4.3.3.6         6.3.4.2         
6.3.5.2         6.3.5.4         6.3.5.5         6.3.5.13
Pathway
rn00220  Arginine biosynthesis
rn00250  Alanine, aspartate and glutamate metabolism
rn00471  D-Glutamine and D-glutamate metabolism
rn01100  Metabolic pathways
Orthology
K01425  glutaminase [EC:3.5.1.2]
K01954  carbamoyl-phosphate synthase [EC:6.3.5.5]
K01955  carbamoyl-phosphate synthase large subunit [EC:6.3.5.5]
K01956  carbamoyl-phosphate synthase small subunit [EC:6.3.5.5]
K05597  glutamin-(asparagin-)ase [EC:3.5.1.38]
K11540  carbamoyl-phosphate synthase / aspartate carbamoyltransferase / dihydroorotase [EC:6.3.5.5 2.1.3.2 3.5.2.3]
K11541  carbamoyl-phosphate synthase / aspartate carbamoyltransferase [EC:6.3.5.5 2.1.3.2]
K23265  phosphoribosylformylglycinamidine synthase subunit PurQ / glutaminase [EC:6.3.5.3 3.5.1.2]
Other DBs
RHEA: 15892

DBGET integrated database retrieval system