KEGG   ORTHOLOGY: K05797Help
Entry
K05797                      KO                                     

Name
pchF
Definition
4-cresol dehydrogenase (hydroxylating) flavoprotein subunit [EC:1.17.99.1]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K05797  pchF; 4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.17  Acting on CH or CH2 groups
   1.17.99  With unknown physiological acceptors
    1.17.99.1  4-methylphenol dehydrogenase (hydroxylating)
     K05797  pchF; 4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
BRITE hierarchy
Other DBs
RN: R02675 R11194
GO: 0018695
Genes
PCQ: PcP3B5_13510(pchF_1) PcP3B5_26440(pchF_2) PcP3B5_26540(pchF_3)
PPUT: L483_22020
PDR: H681_14085
PALK: PSAKL28_20190 PSAKL28_30910 PSAKL28_31390
PSOS: POS17_3788(Neut_0635) POS17_5141(pchF)
PSET: THL1_645
PSIL: PMA3_18570
PAT: Patl_1202
AMB: AMBAS45_13615
CYQ: Q91_0490 Q91_0494
NOC: Noc_2406
NHL: Nhal_1759
NWA: Nwat_1784
OCE: GU3_14310
BYI: BYI23_B003260(pchF)
BUE: BRPE67_BCDS05760(pchF)
BXE: Bxe_A2781
BXB: DR64_464(pchF)
BPH: Bphy_1706
BFN: OI25_3960(pchF)
AFQ: AFA_05565
PNA: Pnap_3838
NEU: NE0741
NET: Neut_1656
EBA: ebA3165(pchF) ebA5380
AZA: AZKH_2917
CCF: YSQ_00025
CCOF: VC76_00030(pchF_2)
CCOO: ATE51_00010(pchF)
CURE: CUREO_0963
SULJ: SJPD1_1025
GME: Gmet_2125(pcmI)
GEO: Geob_0119(pcmI)
GEM: GM21_2847
CCX: COCOR_00350(pchF)
SPHR: BSY17_949
AAY: WYH_02883(pchF_1) WYH_03108(pchF_2) WYH_03116(pchF_3) WYH_03119(pchF_4)
RRU: Rru_A3112
RRF: F11_15945
MAGQ: MGMAQ_0999
SALS: SLNWT_0108
SRN: A4G23_02275(pchF)
STRD: NI25_07845
SMAL: SMALA_8211
MMAR: MODMU_2955(vaoA)
ACTN: L083_7156
MPK: VL20_5235
TTF: THTE_3672
SUS: Acid_2038
NDE: NIDE0022(pchF)
AG: AGO01130(pchF)
 » show all
TaxonomyKoalaUniProt
Reference
PMID:7929007
  Authors
Kim J, Fuller JH, Cecchini G, McIntire WS
  Title
Cloning, sequencing, and expression of the structural genes for the cytochrome and flavoprotein subunits of p-cresol methylhydroxylase from two strains of Pseudomonas putida.
  Journal
J Bacteriol 176:6349-61 (1994)
DOI:10.1128/JB.176.20.6349-6361.1994
Reference
  Authors
Chen YF, Chao H, Zhou NY
  Title
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866.
  Journal
Appl Microbiol Biotechnol 98:1349-56 (2014)
DOI:10.1007/s00253-013-5001-z
  Sequence

KEGG   ORTHOLOGY: K20200Help
Entry
K20200                      KO                                     

Name
pchC
Definition
4-cresol dehydrogenase [hydroxylating] cytochrome subunit
Pathway
ko00623  Toluene degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K20200  pchC; 4-cresol dehydrogenase [hydroxylating] cytochrome subunit
BRITE hierarchy
Other DBs
RN: R02675 R11194
Genes
PPUT: L483_22010
PDR: H681_14075
PALK: PSAKL28_20210 PSAKL28_31410
PSOS: POS17_5139
OCE: GU3_14320
EBA: ebB97(pchC)
TaxonomyKoalaUniProt
Reference
PMID:7929007
  Authors
Kim J, Fuller JH, Cecchini G, McIntire WS
  Title
Cloning, sequencing, and expression of the structural genes for the cytochrome and flavoprotein subunits of p-cresol methylhydroxylase from two strains of Pseudomonas putida.
  Journal
J Bacteriol 176:6349-61 (1994)
DOI:10.1128/JB.176.20.6349-6361.1994
  Sequence

KEGG   ENZYME: 1.17.99.1Help
Entry
EC 1.17.99.1                Enzyme                                 

Name
4-methylphenol dehydrogenase (hydroxylating);
p-cresol-(acceptor) oxidoreductase (hydroxylating);
p-cresol methylhydroxylase;
4-cresol dehydrogenase (hydroxylating)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
BRITE hierarchy
Sysname
4-methylphenol:acceptor oxidoreductase (methyl-hydroxylating)
Reaction(IUBMB)
4-methylphenol + 2 acceptor + H2O = 4-hydroxybenzaldehyde + 2 reduced acceptor [RN:R07223]
Reaction(KEGG)
R07223 > R02675;
(other) R11194
Show
Substrate
4-methylphenol [CPD:C01468];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
4-hydroxybenzaldehyde [CPD:C00633];
reduced acceptor [CPD:C00030]
Comment
A flavocytochrome c (FAD). Phenazine methosulfate can act as acceptor. A quinone methide is probably formed as intermediate. The first hydroxylation forms 4-hydroxybenzyl alcohol; a second hydroxylation converts this into 4-hydroxybenzaldehyde.
History
EC 1.17.99.1 created 1983, modified 2001, modified 2011, modified 2015
Pathway
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05797  4-cresol dehydrogenase (hydroxylating) flavoprotein subunit
Genes
PCQ: PcP3B5_13510(pchF_1) PcP3B5_26440(pchF_2) PcP3B5_26540(pchF_3)
PPUT: L483_22020
PDR: H681_14085
PALK: PSAKL28_20190 PSAKL28_30910 PSAKL28_31390
PSOS: POS17_3788(Neut_0635) POS17_5141(pchF)
PSET: THL1_645
PSIL: PMA3_18570
PAT: Patl_1202
AMB: AMBAS45_13615
CYQ: Q91_0490 Q91_0494
NOC: Noc_2406
NHL: Nhal_1759
NWA: Nwat_1784
OCE: GU3_14310
BYI: BYI23_B003260(pchF)
BUE: BRPE67_BCDS05760(pchF)
BXE: Bxe_A2781
BXB: DR64_464(pchF)
BPH: Bphy_1706
BFN: OI25_3960(pchF)
AFQ: AFA_05565
PNA: Pnap_3838
NEU: NE0741
NET: Neut_1656
EBA: ebA3165(pchF) ebA5380
AZA: AZKH_2917
CCF: YSQ_00025
CCOF: VC76_00030(pchF_2)
CCOO: ATE51_00010(pchF)
CURE: CUREO_0963
SULJ: SJPD1_1025
GME: Gmet_2125(pcmI)
GEO: Geob_0119(pcmI)
GEM: GM21_2847
CCX: COCOR_00350(pchF)
SPHR: BSY17_949
AAY: WYH_02883(pchF_1) WYH_03108(pchF_2) WYH_03116(pchF_3) WYH_03119(pchF_4)
RRU: Rru_A3112
RRF: F11_15945
MAGQ: MGMAQ_0999
SALS: SLNWT_0108
SRN: A4G23_02275(pchF)
STRD: NI25_07845
SMAL: SMALA_8211
MMAR: MODMU_2955(vaoA)
ACTN: L083_7156
MPK: VL20_5235
TTF: THTE_3672
SUS: Acid_2038
NDE: NIDE0022(pchF)
 » show all
Taxonomy
Reference
1  [PMID:588247]
  Authors
Hopper DJ, Taylor DG.
  Title
The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida.
  Journal
Biochem J 167:155-62 (1977)
Reference
2  [PMID:7391034]
  Authors
McIntire W, Edmondson DE, Singer TP, Hopper DJ.
  Title
8 alpha-O-Tyrosyl-FAD: a new form of covalently bound flavin from p-cresol methylhydroxylase.
  Journal
J Biol Chem 255:6553-5 (1980)
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.1
IUBMB Enzyme Nomenclature: 1.17.99.1
ExPASy - ENZYME nomenclature database: 1.17.99.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.17.99.1
BRENDA, the Enzyme Database: 1.17.99.1
CAS: 66772-07-4

KEGG   REACTION: R02675Help
Entry
R02675                      Reaction                               

Name
4-cresol:acceptor oxidoreductase (methyl-hydroxylating)
Definition
4-Cresol + 2 Oxidized ferredoxin + H2O <=> 4-Hydroxybenzaldehyde + 2 Reduced ferredoxin
Equation
Comment
multi-step reaction
possibly intermediates (quinone methide and 4-hydroxybenzyl alcohol)
Reaction class
RC00769  C00633_C01468
Enzyme
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K05797  4-cresol dehydrogenase (hydroxylating) flavoprotein subunit [EC:1.17.99.1]
K20200  4-cresol dehydrogenase [hydroxylating] cytochrome subunit

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