| Entry |
|
|---|
| Name |
[amino-group carrier protein]---L-2-aminoadipate ligase;
alpha-aminoadipate-lysW ligase;
lysX (gene name);
LysX (ambiguous);
AAA---LysW ligase;
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming);
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase
|
|---|
| Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
|
|---|
| Sysname |
[amino-group carrier protein]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming)
|
|---|
| Reaction(IUBMB) |
ATP + an [amino-group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + an [amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
ATP [CPD: C00002];
[amino-group carrier protein]-C-terminal-L-glutamate;
L-2-aminoadipate [CPD: C00956]
|
|---|
| Product |
ADP [CPD: C00008];
phosphate [CPD: C00009];
[amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]
|
|---|
| Comment |
The enzymes from the thermophilic bacterium Thermus thermophilus and the thermophilic archaea Sulfolobus acidocaldarius and Sulfolobus tokodaii protect the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW. This reaction is part of the lysine biosynthesis pathway in these organisms.
|
|---|
| History |
EC 6.3.2.43 created 2014, modified 2019
|
|---|
| Pathway |
|
|---|
| Orthology |
| K05827 | [lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase |
|
|---|
| Genes |
» show all
|
|---|
| Reference |
|
|---|
| Authors |
Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S |
|---|
| Title |
Cloning, expression, purification, crystallization and initial crystallographic analysis of the lysine-biosynthesis LysX protein from Thermus thermophilus HB8. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M |
|---|
| Title |
Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M |
|---|
| Title |
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.3.2.43 |
| ExPASy - ENZYME nomenclature database: | 6.3.2.43 |
|
|---|
