KEGG   ORTHOLOGY: K05942
Entry
K05942                      KO                                     

Name
E2.3.3.3
Definition
citrate (Re)-synthase [EC:2.3.3.3]
Pathway
ko00020  Citrate cycle (TCA cycle)
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01210  2-Oxocarboxylic acid metabolism
ko01230  Biosynthesis of amino acids
Module
M00009  Citrate cycle (TCA cycle, Krebs cycle)
M00010  Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00020 Citrate cycle (TCA cycle)
    K05942  E2.3.3.3; citrate (Re)-synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.3  Acyl groups converted into alkyl groups on transfer
    2.3.3.3  citrate (Re)-synthase
     K05942  E2.3.3.3; citrate (Re)-synthase
Other DBs
RN: R00351
COG: COG0119
GO: 0050450
Genes
DVU: DVU0398
DVL: Dvul_2536
DVM: DvMF_1549
DVG: Deval_0360
DDE: Dde_0520
DDS: Ddes_0102
DGG: DGI_0640
DFI: AXF13_13150
DPG: DESPIGER_1798
DEF: CNY67_00565
DTR: RSDT_0090
DFL: DFE_2759
DRT: Dret_2092
SAT: SYN_02536
CAC: CA_C0970
CAE: SMB_G0987(leuA1)
CAY: CEA_G0981
CBO: CBO0417
CBA: CLB_0450
CBH: CLC_0483
CBY: CLM_0496
CBL: CLK_3619
CBB: CLD_0326
CBI: CLJ_B0485
CBF: CLI_0502
CKL: CKL_0973
CKR: CKR_0877
CPAS: Clopa_3429
CPAT: CLPA_c14670(leuA2)
CPAE: CPAST_c14670(leuA2)
CSQ: CSCA_3672
CLD: CLSPO_c04550(leuA)
CTYK: CTK_C06610
CTH: Cthe_3157
HSC: HVS_12360(leuA3)
CCE: Ccel_2177
BPB: bpr_I1100
BHU: bhn_I1070
RIX: RO1_15050
RIM: ROI_22460
BPRO: PMF13cell1_03741(leuA_2)
CPY: Cphy_3097
HSD: SD1D_0728
CPRO: CPRO_08880(leuA_1)
EHL: EHLA_0853
ERT: EUR_16330
ERA: ERE_32270
DET: DET1614
DEH: cbdbA1708
DMC: btf_1554
DMD: dcmb_1500
DMG: GY50_1509
DFO: Dform_00040(nifV)
DGE: Dgeo_2246
DFC: DFI_11390
KST: KSMBR1_2157(cimA)
ALUS: STSP2_00175(leuA_2)
EPO: Epro_0887
CTHI: THC_1672
MBAR: MSBR2_3146
MBAK: MSBR3_3141
MAC: MA_4264
MMAC: MSMAC_0304
METM: MSMTP_0335
MTHR: MSTHT_1587
MTHE: MSTHC_1702
MHOR: MSHOH_0397
MBU: Mbur_1075
MMET: MCMEM_1605
MMH: Mmah_0581
MPY: Mpsy_1028
MTP: Mthe_0336
MPD: MCP_0446
MEZ: Mtc_1389
RCI: RCIX1793(leuA-3)
IHO: Igni_0261
IIS: EYM_04445
CMA: Cmaq_1917
VDI: Vdis_0198
VMO: VMUT_1536
KCR: Kcr_0973
LOKI: Lokiarch_26360(leuA_2) Lokiarch_35110(leuA_4)
 » show all
Reference
  Authors
Li F, Hagemeier CH, Seedorf H, Gottschalk G, Thauer RK
  Title
Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase.
  Journal
J Bacteriol 189:4299-304 (2007)
DOI:10.1128/JB.00198-07
  Sequence
[ckl:CKL_0973]
Reference
  Authors
Marco-Urrea E, Paul S, Khodaverdi V, Seifert J, von Bergen M, Kretzschmar U, Adrian L
  Title
Identification and characterization of a re-citrate synthase in Dehalococcoides strain CBDB1.
  Journal
J Bacteriol 193:5171-8 (2011)
DOI:10.1128/JB.05120-11
  Sequence
[deh:cbdbA1708]

KEGG   ENZYME: 2.3.3.3
Entry
EC 2.3.3.3                  Enzyme                                 

Name
citrate (Re)-synthase;
(R)-citrate synthase;
Re-citrate-synthase;
citrate oxaloacetate-lyase [(pro-3R)-CH2COO-->acetyl-CoA]
Class
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
Sysname
acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-R)-carboxymethyl-forming]
Reaction(IUBMB)
acetyl-CoA + H2O + oxaloacetate = citrate + CoA [RN:R00351]
Reaction(KEGG)
R00351
Substrate
acetyl-CoA [CPD:C00024];
H2O [CPD:C00001];
oxaloacetate [CPD:C00036]
Product
citrate [CPD:C00158];
CoA [CPD:C00010]
Comment
This enzyme is inactivated by oxygen and is found in some anaerobes. Its stereospecificity is opposite to that of EC 2.3.3.1, citrate (Si)-synthase.
History
EC 2.3.3.3 created 1972 as EC 4.1.3.28, transferred 2002 to EC 2.3.3.3
Pathway
ec00020  Citrate cycle (TCA cycle)
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K05942  citrate (Re)-synthase
Genes
DVU: DVU0398
DVL: Dvul_2536
DVM: DvMF_1549
DVG: Deval_0360
DDE: Dde_0520
DDS: Ddes_0102
DGG: DGI_0640
DFI: AXF13_13150
DPG: DESPIGER_1798
DEF: CNY67_00565
DTR: RSDT_0090
DFL: DFE_2759
DRT: Dret_2092
SAT: SYN_02536
CAC: CA_C0970
CAE: SMB_G0987(leuA1)
CAY: CEA_G0981
CBO: CBO0417
CBA: CLB_0450
CBH: CLC_0483
CBY: CLM_0496
CBL: CLK_3619
CBB: CLD_0326
CBI: CLJ_B0485
CBF: CLI_0502
CKL: CKL_0973
CKR: CKR_0877
CPAS: Clopa_3429
CPAT: CLPA_c14670(leuA2)
CPAE: CPAST_c14670(leuA2)
CSQ: CSCA_3672
CLD: CLSPO_c04550(leuA)
CTYK: CTK_C06610
CTH: Cthe_3157
HSC: HVS_12360(leuA3)
CCE: Ccel_2177
BPB: bpr_I1100
BHU: bhn_I1070
RIX: RO1_15050
RIM: ROI_22460
BPRO: PMF13cell1_03741(leuA_2)
CPY: Cphy_3097
HSD: SD1D_0728
CPRO: CPRO_08880(leuA_1)
EHL: EHLA_0853
ERT: EUR_16330
ERA: ERE_32270
DET: DET1614
DEH: cbdbA1708
DMC: btf_1554
DMD: dcmb_1500
DMG: GY50_1509
DFO: Dform_00040(nifV)
DGE: Dgeo_2246
DFC: DFI_11390
KST: KSMBR1_2157(cimA)
ALUS: STSP2_00175(leuA_2)
EPO: Epro_0887
CTHI: THC_1672
MBAR: MSBR2_3146
MBAK: MSBR3_3141
MAC: MA_4264
MMAC: MSMAC_0304
METM: MSMTP_0335
MTHR: MSTHT_1587
MTHE: MSTHC_1702
MHOR: MSHOH_0397
MBU: Mbur_1075
MMET: MCMEM_1605
MMH: Mmah_0581
MPY: Mpsy_1028
MTP: Mthe_0336
MPD: MCP_0446
MEZ: Mtc_1389
RCI: RCIX1793(leuA-3)
IHO: Igni_0261
IIS: EYM_04445
CMA: Cmaq_1917
VDI: Vdis_0198
VMO: VMUT_1536
KCR: Kcr_0973
LOKI: Lokiarch_26360(leuA_2) Lokiarch_35110(leuA_4)
 » show all
Reference
1  [PMID:5808294]
  Authors
Dittbrenner S, Chowdhury AA, Gottschalk G.
  Title
The stereospecificity of the (R)-citrates synthase in the presence of p-chloromercuribenzoate.
  Journal
Biochem Biophys Res Commun 36:802-8 (1969)
DOI:10.1016/0006-291X(69)90680-9
Reference
2  [PMID:4974734]
  Authors
Gottschalk G.
  Title
Partial purification and some properties of the (R)-citrate synthase from Clostridium acidi-urici.
  Journal
Eur J Biochem 7:301-6 (1969)
DOI:10.1111/j.1432-1033.1969.tb19607.x
Reference
3  [PMID:5958189]
  Authors
Gottschalk G, Barker HA.
  Title
Synthesis of glutamate and citrate by Clostridium kluyveri. A new type of citrate synthase.
  Journal
Biochemistry 5:1125-33 (1966)
DOI:10.1021/bi00868a003
Other DBs
ExplorEnz - The Enzyme Database: 2.3.3.3
IUBMB Enzyme Nomenclature: 2.3.3.3
ExPASy - ENZYME nomenclature database: 2.3.3.3
BRENDA, the Enzyme Database: 2.3.3.3
CAS: 9077-70-7

KEGG   REACTION: R00351
Entry
R00351                      Reaction                               

Name
acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
Definition
Citrate + CoA <=> Acetyl-CoA + H2O + Oxaloacetate
Equation
Comment
(pro-S)-carboxymethyl-forming (ec 2.3.3.1)
(pro-R)-carboxymethyl-forming (ec 2.3.3.3)
unknown stereospecificity (ec 2.3.3.16)
Reaction class
RC00004  C00010_C00024
RC00067  C00036_C00158
Enzyme
2.3.3.1         2.3.3.3         2.3.3.16
Pathway
rn00020  Citrate cycle (TCA cycle)
rn00630  Glyoxylate and dicarboxylate metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
rn01210  2-Oxocarboxylic acid metabolism
rn01230  Biosynthesis of amino acids
Module
M00009  Citrate cycle (TCA cycle, Krebs cycle)
M00010  Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate
M00012  Glyoxylate cycle
M00740  Methylaspartate cycle
Orthology
K01647  citrate synthase [EC:2.3.3.1]
K05942  citrate (Re)-synthase [EC:2.3.3.3]
Other DBs
RHEA: 16848

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