KEGG ORTHOLOGY: K07419

ORTHOLOGY: K07419

Entry

K07419 KO

Name

CYP2R1

Definition

vitamin D 25-hydroxylase [EC:1.14.14.24]

Pathway

ko00100

Steroid biosynthesis

ko01100

Metabolic pathways

ko01110

Biosynthesis of secondary metabolites

Module

M00103

Cholecalciferol biosynthesis

Disease

H01143

Vitamin D-dependent rickets

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K07419  CYP2R1; vitamin D 25-hydroxylase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K07419  CYP2R1; vitamin D 25-hydroxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.24  vitamin D 25-hydroxylase
     K07419  CYP2R1; vitamin D 25-hydroxylase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, animal type
  CYP2 family
   K07419  CYP2R1; vitamin D 25-hydroxylase

Other DBs

RN:

R03611

GO:

0030343

Genes

HSA:	120227(CYP2R1)

PTR:	451042(CYP2R1)

PPS:

GGO:

PON:

NLE:

MCC:	698834(CYP2R1)

MCF:

102138775

CSAB:

103239784(CYP2R1)

RRO:

104681937

RBB:

108535517

CJC:	100394527(CYP2R1)

SBQ:

101037293

MMU:	244209(Cyp2r1)

MCAL:

110298348

MPAH:

110332047

RNO:	361631(Cyp2r1)

MUN:

CGE:

NGI:

HGL:

CCAN:

OCU:

TUP:

CFA:	612993(CYP2R1)

VVP:

AML:

UMR:

UAH:

ORO:

ELK:

FCA:

PTG:

PPAD:

AJU:

BTA:	541302(CYP2R1)

BOM:

BIU:

BBUB:

CHX:

OAS:

SSC:	100124375(CYP2R1)

CFR:

CDK:

BACU:

LVE:

OOR:

DLE:

PCAD:

ECB:	100146381(CYP2R1)

EPZ:

EAI:

MYB:

MYD:

MNA:

HAI:

DRO:

PALE:

RAY:

MJV:

LAV:

TMU:

MDO:

SHR:

PCW:

OAA:

GGA:	423065(CYP2R1)

MGP:	100547524 104910999

CJO:

NMEL:

APLA:

ACYG:

TGU:

LSR:

SCAN:

GFR:

FAB:

PHI:

PMAJ:

CCW:

ETL:

FPG:

FCH:

CLV:

EGZ:

NNI:

ACUN:

PADL:

AAM:

ASN:

AMJ:	102564928(CYP2R1)

PSS:

CMY:

CPIC:

ACS:

PVT:

PBI:

PMUR:

TSR:

PMUA:

GJA:

XLA:	108713716(cyp2r1.L) 108715308(cyp2r1.S)

XTR:	100127674(cyp2r1)

NPR:

108802409

DRE:	563369(cyp2r1)

SRX:

107750548

SANH:

107688167 107700616

SGH:	107585944 107594282

CCAR:

109094296

IPU:	108271345(Cyp2r1)

PHYP:

AMEX:

EEE:

TRU:

TNG:	GSTEN00017808G001

LCO:

NCC:

MZE:

ONL:

OLA:

XMA:

XCO:

PRET:

CVG:

NFU:

KMR:

ALIM:

AOCE:

CSEM:

POV:

LCF:

SDU:

SLAL:

HCQ:

BPEC:

MALB:

SASA:

OTW:	112235131 112263818

SALP:

111969491 111975524

ELS:

105022275

SFM:	108934550 108940274

PKI:

111842773

LCM:

102347406

CMK:

103175087

RTP:	109926988 109927197

CSCU:

111619042 111629140 111629227

PCAN:

112556695

MRR:	Moror_16154

» show all

Reference

PMID:15465040

Authors

Shinkyo R, Sakaki T, Kamakura M, Ohta M, Inouye K

Title

Metabolism of vitamin D by human microsomal CYP2R1.

Journal

Biochem Biophys Res Commun 324:451-7 (2004)
DOI:10.1016/j.bbrc.2004.09.073

Reference

PMID:12867411

Authors

Cheng JB, Motola DL, Mangelsdorf DJ, Russell DW

Title

De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxilase.

Journal

J Biol Chem 278:38084-93 (2003)
DOI:10.1074/jbc.M307028200

Sequence

[hsa:120227] [mmu:244209]

ENZYME: 1.14.14.24

Entry

EC 1.14.14.24 Enzyme

Name

vitamin D 25-hydroxylase;
vitamin D2 25-hydroxylase;
vitamin D3 25-hydroxylase;
CYP2R1

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

calciol,NADPH---hemoprotein reductase:oxygen oxidoreductase (25-hydroxylating)

Reaction(IUBMB)

calciol + O2 + [reduced NADPH---hemoprotein reductase] = calcidiol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03611]

Reaction(KEGG)

R03611

Substrate

calciol [CPD:C05443];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]

Product

calcidiol [CPD:C01561];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A microsomal enzyme isolated from human and mouse liver that bioactivates vitamin D3. While multiple isoforms (CYP27A1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) are able to catalyse the reaction in vitro, only CYP2R1 is thought to catalyse the reaction in humans in vivo [4]. The direct electron donor to the enzyme is EC 1.6.2.4, NADPH---hemoprotein reductase.

History

EC 1.14.14.24 created 2012 as EC 1.14.13.159, transferred 2016 to EC 1.14.14.24

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K07419

vitamin D 25-hydroxylase

Genes

HSA:	120227(CYP2R1)

PTR:	451042(CYP2R1)

PPS:

GGO:

PON:

NLE:

MCC:	698834(CYP2R1)

MCF:

102138775

CSAB:

103239784(CYP2R1)

RRO:

104681937

RBB:

108535517

CJC:	100394527(CYP2R1)

SBQ:

101037293

MMU:	244209(Cyp2r1)

MCAL:

110298348

MPAH:

110332047

RNO:	361631(Cyp2r1)

MUN:

CGE:

NGI:

HGL:

CCAN:

OCU:

TUP:

CFA:	612993(CYP2R1)

VVP:

AML:

UMR:

UAH:

ORO:

ELK:

FCA:

PTG:

PPAD:

AJU:

BTA:	541302(CYP2R1)

BOM:

BIU:

BBUB:

CHX:

OAS:

SSC:	100124375(CYP2R1)

CFR:

CDK:

BACU:

LVE:

OOR:

DLE:

PCAD:

ECB:	100146381(CYP2R1)

EPZ:

EAI:

MYB:

MYD:

MNA:

HAI:

DRO:

PALE:

RAY:

MJV:

LAV:

TMU:

MDO:

SHR:

PCW:

OAA:

GGA:	423065(CYP2R1)

MGP:	100547524 104910999

CJO:

NMEL:

APLA:

ACYG:

TGU:

LSR:

SCAN:

GFR:

FAB:

PHI:

PMAJ:

CCW:

ETL:

FPG:

FCH:

CLV:

EGZ:

NNI:

ACUN:

PADL:

AAM:

ASN:

AMJ:	102564928(CYP2R1)

PSS:

CMY:

CPIC:

ACS:

PVT:

PBI:

PMUR:

TSR:

PMUA:

GJA:

XLA:	108713716(cyp2r1.L) 108715308(cyp2r1.S)

XTR:	100127674(cyp2r1)

NPR:

108802409

DRE:	563369(cyp2r1)

SRX:

107750548

SANH:

107688167 107700616

SGH:	107585944 107594282

CCAR:

109094296

IPU:	108271345(Cyp2r1)

PHYP:

AMEX:

EEE:

TRU:

TNG:	GSTEN00017808G001

LCO:

NCC:

MZE:

ONL:

OLA:

XMA:

XCO:

PRET:

CVG:

NFU:

KMR:

ALIM:

AOCE:

CSEM:

POV:

LCF:

SDU:

SLAL:

HCQ:

BPEC:

MALB:

SASA:

OTW:	112235131 112263818

SALP:

111969491 111975524

ELS:

105022275

SFM:	108934550 108940274

PKI:

111842773

LCM:

102347406

CMK:

103175087

RTP:	109926988 109927197

CSCU:

111619042 111629140 111629227

PCAN:

112556695

MRR:	Moror_16154

» show all

Reference

1 [PMID:12867411]

Authors

Cheng JB, Motola DL, Mangelsdorf DJ, Russell DW

Title

De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxilase.

Journal

J Biol Chem 278:38084-93 (2003)
DOI:10.1074/jbc.M307028200

Sequence

[hsa:120227] [mmu:244209]

Reference

2 [PMID:15465040]

Authors

Shinkyo R, Sakaki T, Kamakura M, Ohta M, Inouye K.

Title

Metabolism of vitamin D by human microsomal CYP2R1.

Journal

Biochem Biophys Res Commun 324:451-7 (2004)
DOI:10.1016/j.bbrc.2004.09.073

Sequence

[hsa:120227]

Reference

3 [PMID:18511070]

Authors

Strushkevich N, Usanov SA, Plotnikov AN, Jones G, Park HW

Title

Structural analysis of CYP2R1 in complex with vitamin D3.

Journal

J Mol Biol 380:95-106 (2008)
DOI:10.1016/j.jmb.2008.03.065

Sequence

[hsa:120227]

Reference

4 [PMID:22310641]

Authors

Zhu J, Deluca HF

Title

Vitamin D 25-hydroxylase - Four decades of searching, are we there yet?

Journal

Arch Biochem Biophys 523:30-6 (2012)
DOI:10.1016/j.abb.2012.01.013

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.24

IUBMB Enzyme Nomenclature:

1.14.14.24

ExPASy - ENZYME nomenclature database:

1.14.14.24

BRENDA, the Enzyme Database:

1.14.14.24

REACTION: R03611

Entry

R03611 Reaction

Name

calciol,NADPH-hemoprotein reductase:oxygen oxidoreductase (25-hydroxylating)

Definition

Calcidiol + [Oxidized NADPH---hemoprotein reductase] + H2O <=> Vitamin D3 + [Reduced NADPH---hemoprotein reductase] + Oxygen

Equation

C01561 + C03161 + C00001 <=> C05443 + C03024 + C00007

Reaction class

RC00963

C01561_C05443

Enzyme

1.14.14.24

Pathway

rn00100

Steroid biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00103

Cholecalciferol biosynthesis

Orthology

K07419

vitamin D 25-hydroxylase [EC:1.14.14.24]

Other DBs

RHEA:

32906

All links

Ontology (1)   
   KEGG BRITE (1)   
Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG ENZYME (1)   
   KEGG RCLASS (1)   
Gene (161)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (160)   
All databases (177)   

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