KEGG   ORTHOLOGY: K07540Help
Entry
K07540                      KO                                     

Name
E4.1.99.11
Definition
benzylsuccinate synthase [EC:4.1.99.11]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07540  E4.1.99.11; benzylsuccinate synthase
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Aromatics degradation
    M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
     K07540  E4.1.99.11; benzylsuccinate synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.99  Other carbon-carbon lyases
    4.1.99.11  benzylsuccinate synthase
     K07540  E4.1.99.11; benzylsuccinate synthase
BRITE hierarchy
Other DBs
RN: R05598
GO: 0018805
Genes
EBA: c2A303(bssC) c2A304(bssA) c2A305(bssB)
AZI: AzCIB_4504 AzCIB_4505 AzCIB_4506
GME: Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: Dalk_2198
DTO: TOL2_C01390(bssB) TOL2_C01400(bssA) TOL2_C01410(bssC)
DGI: Desgi_0659 Desgi_0660
AG: CAA05051(bssC) CAA05052(bssA) CAA05053(bssB)
TaxonomyKoalaUniProt
Reference
PMID:9632263
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol Microbiol 28:615-28 (1998)
DOI:10.1046/j.1365-2958.1998.00826.x
  Sequence

KEGG   ENZYME: 4.1.99.11Help
Entry
EC 4.1.99.11                Enzyme                                 

Name
benzylsuccinate synthase;
benzylsuccinate fumarate-lyase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
benzylsuccinate fumarate-lyase (toluene-forming)
Reaction(IUBMB)
benzylsuccinate = toluene + fumarate [RN:R05598]
Reaction(KEGG)
Substrate
benzylsuccinate [CPD:C09816]
Product
toluene [CPD:C01455];
fumarate [CPD:C00122]
Comment
A glycyl radical enzyme that is inhibited by benzyl alcohol, benzaldehyde, phenylhydrazine and is inactivated by oxygen.
History
EC 4.1.99.11 created 2000
Pathway
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K07540  benzylsuccinate synthase
Genes
EBA: c2A303(bssC) c2A304(bssA) c2A305(bssB)
AZI: AzCIB_4504 AzCIB_4505 AzCIB_4506
GME: Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: Dalk_2198
DTO: TOL2_C01390(bssB) TOL2_C01400(bssA) TOL2_C01410(bssC)
DGI: Desgi_0659 Desgi_0660
Taxonomy
Reference
1  [PMID:9765580]
  Authors
Beller HR, Spormann AM.
  Title
Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.
  Journal
J Bacteriol 180:5454-7 (1998)
Reference
2  [PMID:9632263]
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J.
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol Microbiol 28:615-28 (1998)
DOI:10.1046/j.1365-2958.1998.00826.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.11
IUBMB Enzyme Nomenclature: 4.1.99.11
ExPASy - ENZYME nomenclature database: 4.1.99.11
UM-BBD (Biocatalysis/Biodegradation Database): 4.1.99.11
BRENDA, the Enzyme Database: 4.1.99.11
CAS: 209264-18-6

KEGG   REACTION: R05598Help
Entry
R05598                      Reaction                               

Name
Benzylsuccinate fumarate-lyase
Definition
Toluene + Fumarate <=> Benzylsuccinate
Equation
Comment
BssA,BssB,BssC
Reaction class
RC01433  C00122_C09816
RC01434  C01455_C09816
Enzyme
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00418  Toluene degradation, anaerobic, toluene => benzoyl-CoA
Orthology
K07540  benzylsuccinate synthase [EC:4.1.99.11]
Other DBs
RHEA: 10419

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