KEGG   ORTHOLOGY: K07824
Entry
K07824                      KO                                     

Name
CYP53A1
Definition
benzoate 4-monooxygenase [EC:1.14.14.92]
Pathway
ko00362  Benzoate degradation
ko00627  Aminobenzoate degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K07824  CYP53A1; benzoate 4-monooxygenase
   00627 Aminobenzoate degradation
    K07824  CYP53A1; benzoate 4-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K07824  CYP53A1; benzoate 4-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.92  benzoate 4-monooxygenase
     K07824  CYP53A1; benzoate 4-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP53 family
   K07824  CYP53A1; benzoate 4-monooxygenase
Other DBs
RN: R01295
GO: 0018664
Genes
QSU: 112021863 112023807
NCR: NCU01086
NTE: NEUTE1DRAFT98143(NEUTE1DRAFT_98143)
SMP: SMAC_03160
PAN: PODANSg8616
TTT: THITE_2107910
MTM: MYCTH_2301715
MGR: MGG_13928
SSCK: SPSK_09137
FGR: FGSG_08079 FGSG_10451 FGSG_13445
MAW: MAC_01732
CMT: CCM_08604
MBE: MBM_02231
ANG: ANI_1_418084(An09g03500)
PBL: PAAG_11325(PAAG_01629)
PTE: PTT_11926
ZTR: MYCGRDRAFT_53213(CYP-69)
TASA: A1Q1_06680
ABP: AGABI1DRAFT115220(AGABI1DRAFT_115220) AGABI1DRAFT115340(AGABI1DRAFT_115340)
ABV: AGABI2DRAFT194181(AGABI2DRAFT_194181) AGABI2DRAFT194303(AGABI2DRAFT_194303)
 » show all
Reference
PMID:2250647
  Authors
van Gorcom RF, Boschloo JG, Kuijvenhoven A, Lange J, van Vark AJ, Bos CJ, van Balken JA, Pouwels PH, van den Hondel CA
  Title
Isolation and molecular characterisation of the benzoate-para-hydroxylase gene (bphA) of Aspergillus niger: a member of a new gene family of the cytochrome P450 superfamily.
  Journal
Mol Gen Genet 223:192-7 (1990)
DOI:10.1007/BF00265053
  Sequence

KEGG   ENZYME: 1.14.14.92
Entry
EC 1.14.14.92               Enzyme                                 

Name
benzoate 4-monooxygenase;
benzoic acid 4-hydroxylase;
benzoate 4-hydroxylase;
benzoic 4-hydroxylase;
benzoate-p-hydroxylase;
p-hydroxybenzoate hydroxylase;
CYP53A1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
benzoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
benzoate + [reduced NADPH---hemoprotein reductase] + O2 = 4-hydroxybenzoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R01295]
Reaction(KEGG)
R01295
Substrate
benzoate [CPD:C00180];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
4-hydroxybenzoate [CPD:C00156];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in Aspergillus fungi.
History
EC 1.14.14.92 created 1976 as EC 1.14.13.12, transferred 2018 to EC 1.14.14.92
Pathway
ec00362  Benzoate degradation
ec00627  Aminobenzoate degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K07824  benzoate 4-monooxygenase
Genes
QSU: 112021863 112023807
NCR: NCU01086
NTE: NEUTE1DRAFT98143(NEUTE1DRAFT_98143)
SMP: SMAC_03160
PAN: PODANSg8616
TTT: THITE_2107910
MTM: MYCTH_2301715
MGR: MGG_13928
SSCK: SPSK_09137
FGR: FGSG_08079 FGSG_10451 FGSG_13445
MAW: MAC_01732
CMT: CCM_08604
MBE: MBM_02231
ANG: ANI_1_418084(An09g03500)
PBL: PAAG_11325(PAAG_01629)
PTE: PTT_11926
ZTR: MYCGRDRAFT_53213(CYP-69)
TASA: A1Q1_06680
ABP: AGABI1DRAFT115220(AGABI1DRAFT_115220) AGABI1DRAFT115340(AGABI1DRAFT_115340)
ABV: AGABI2DRAFT194181(AGABI2DRAFT_194181) AGABI2DRAFT194303(AGABI2DRAFT_194303)
 » show all
Reference
1  [PMID:236777]
  Authors
Reddy CC, Vaidyanathan CS.
  Title
Purification, properties and induction of a specific benzoate-4-hydroxylase from Aspergillus niger (UBC 814).
  Journal
Biochim Biophys Acta 384:46-57 (1975)
DOI:10.1016/0005-2744(75)90094-7
Reference
2  [PMID:11594739]
  Authors
Faber BW, van Gorcom RF, Duine JA
  Title
Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger.
  Journal
Arch Biochem Biophys 394:245-54 (2001)
DOI:10.1006/abbi.2001.2534
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.92
IUBMB Enzyme Nomenclature: 1.14.14.92
ExPASy - ENZYME nomenclature database: 1.14.14.92
BRENDA, the Enzyme Database: 1.14.14.92
CAS: 39391-25-8

KEGG   REACTION: R01295
Entry
R01295                      Reaction                               

Name
benzoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
Definition
Benzoate + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 4-Hydroxybenzoate + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC00490  C00156_C00180
Enzyme
Pathway
rn00362  Benzoate degradation
rn00627  Aminobenzoate degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Orthology
K07824  benzoate 4-monooxygenase [EC:1.14.14.92]
Other DBs
RHEA: 18036

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