KEGG   ORTHOLOGY: K08685
Entry
K08685                      KO                                     

Name
qhpA
Definition
quinohemoprotein amine dehydrogenase [EC:1.4.9.1]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K08685  qhpA; quinohemoprotein amine dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.9  With a copper protein as acceptor
    1.4.9.1  methylamine dehydrogenase (amicyanin)
     K08685  qhpA; quinohemoprotein amine dehydrogenase
Other DBs
RN: R00606
GO: 0030058
Genes
PRE: PCA10_32510(peaA)
PCQ: PcP3B5_29870
PPU: PP_5602(peaA)
PPF: Pput_2307
PPG: PputGB1_2475
PPW: PputW619_2945
PPT: PPS_2940
PPB: PPUBIRD1_2330
PPI: YSA_11298
PPX: T1E_1613
PPUH: B479_14560
PPUT: L483_18160
PPUN: PP4_23960(peaA)
PPUD: DW66_3200
PMON: X969_14050
PMOT: X970_13695
PFL: PFL_4120(peaA)
PPRO: PPC_4221(peaA)
PMAN: OU5_1439
PEN: PSEEN2977
PKC: PKB_2912
PSEM: TO66_21570
PSOS: POS17_4220(peaA)
PSET: THL1_3176
PSIL: PMA3_20245
PKE: DLD99_19475(peaA)
PUM: HGP31_22220(peaA)
HVN: EI420_18585(peaA)
NCU: F0U83_05970(peaA) F0U83_06020(peaA)
PSE: NH8B_2195
BCEN: DM39_4450(peaA)
BCT: GEM_5082
BPSL: WS57_10400
HPSE: HPF_21410
EBA: ebA2235(qhpA) ebA5178(qhpA)
AZO: azo1239(qhpA)
AOA: dqs_1354
AZA: AZKH_3023(qhpA)
AZD: CDA09_16050(peaA)
AZQ: G3580_00160(peaA)
TCL: Tchl_2123
ABU: Abu_0465
ABT: ABED_0440
AELL: AELL_0619
ASUI: ASUIS_0583
AMOL: AMOL_1804
ARC: ABLL_0607
DTO: TOL2_C06430(qhpA)
BLAG: BLTE_16570
PDE: Pden_1703
PYE: A6J80_01745(peaA)
PZH: CX676_03775(peaA)
PARO: CUV01_13130(peaA)
PKD: F8A10_18985(peaA)
PPAN: ESD82_08405(peaA)
LEJ: ETW24_23980(peaA)
LAQU: R2C4_20365(peaA)
RHC: RGUI_4107
LABR: CHH27_09745(peaA)
LABP: FJ695_27525(peaA)
THAS: C6Y53_00400(peaA)
NAR: Saro_3028
NOT: C7W88_08020(peaA)
NOR: FA702_12465(peaA)
SSAN: NX02_15530
SPHR: BSY17_2364(peaA)
SHYD: CJD35_17865(peaA)
SYA: A6768_01720(peaA)
ALB: AEB_P3055
MAGX: XM1_2531
AZZ: DEW08_00570(peaA)
ECOG: FIV45_14145(peaA) FIV45_16005(peaA)
BACO: OXB_3506
NMK: CHR53_12040(peaA)
TALB: FTW19_10610(peaA)
 » show all

KEGG   ORTHOLOGY: K15228
Entry
K15228                      KO                                     

Name
mauA
Definition
methylamine dehydrogenase light chain [EC:1.4.9.1]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K15228  mauA; methylamine dehydrogenase light chain
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.9  With a copper protein as acceptor
    1.4.9.1  methylamine dehydrogenase (amicyanin)
     K15228  mauA; methylamine dehydrogenase light chain
Other DBs
RN: R00606
GO: 0030058
Genes
YMO: HRD69_07515
VFU: vfu_A02018
VFL: AL536_14010
PMK: MDS_4216
PRE: PCA10_23450(mauA) PCA10_34280(mauA)
PCQ: PcP3B5_30140(aauA)
PPG: PputGB1_2221
PEN: PSEEN2555
PKC: PKB_2656(mauA)
PSET: THL1_3125 THL1_3144
PAT: Patl_0157
GAI: IMCC3135_19700(aauA)
AXE: P40_02480
BCN: Bcen_3308
BCJ: BCAM2301
BCEO: I35_6192
BAM: Bamb_4469
BMU: Bmul_3583
BMK: DM80_4968(aauA)
BMUL: NP80_4958(aauA)
BCT: GEM_3383
BCED: DM42_5689(aauA)
BDL: AK34_3492(aauA)
BCON: NL30_01060
BTEI: WS51_08585
BSEM: WJ12_31395
BPSL: WS57_02725
BMEC: WJ16_29835
BSTG: WT74_30205
BPH: Bphy_3812
AXX: ERS451415_01938(aauA)
AMIM: MIM_c29740(mauA)
MPT: Mpe_A2653
MFA: Mfla_0551
MMB: Mmol_1572
AZO: azo2257(mauA)
AOA: dqs_2388
RBS: RHODOSMS8_03492(aauA)
MEA: Mex_1p2773(mauA)
MCH: Mchl_0562
METD: C0214_27025(mauA)
PHL: KKY_2942
FIL: BN1229_v1_3768(mauA)
FIY: BN1229_v1_3758(mauA)
PLEO: OHA_1_02005(MadB)
CAK: Caul_0560
PDE: Pden_4733
PYE: A6J80_22010(mauA)
PKD: F8A10_20020(mauA)
CID: P73_3335
HBA: Hbal_2737
SMAZ: LH19_18405
SPHD: HY78_09300
SPHM: G432_07310
SSAN: NX02_15540
SPHR: BSY17_2810(mauA)
GDI: GDI0273(mauA) GDI1807(mauA)
APK: APA386B_1939(mauA)
ASZ: ASN_2156(mauA)
APOM: CPF11_05025(mauA)
ATO: CIW82_01900(mauA)
ACET: DS739_02410(mauA)
AOY: EOV40_002260(mauA)
NTN: D5366_10145(mauA)
CPRE: Csp1_26450(mauA)
RER: RER_53630
REY: O5Y_25460
REQ: REQ_44990(mauA)
RHS: A3Q41_02066(mauA)
RRT: 4535765_00133(mauA)
RCR: NCTC10994_01857(mauA)
MIN: Minf_2000
MKC: kam1_317
 » show all
Reference
PMID:2121141
  Authors
Chistoserdov AY, Tsygankov YD, Lidstrom ME
  Title
Cloning and sequencing of the structural gene for the small subunit of methylamine dehydrogenase from Methylobacterium extorquens AM1: evidence for two  tryptophan residues involved in the active center.
  Journal
Biochem Biophys Res Commun 172:211-6 (1990)
DOI:10.1016/s0006-291x(05)80195-0
  Sequence

KEGG   ORTHOLOGY: K15229
Entry
K15229                      KO                                     

Name
mauB
Definition
methylamine dehydrogenase heavy chain [EC:1.4.9.1]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K15229  mauB; methylamine dehydrogenase heavy chain
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.9  With a copper protein as acceptor
    1.4.9.1  methylamine dehydrogenase (amicyanin)
     K15229  mauB; methylamine dehydrogenase heavy chain
Other DBs
RN: R00606
GO: 0030058
Genes
YMO: HRD69_07500
VFU: vfu_A02015
VFL: AL536_13995
PMK: MDS_4213
PRE: PCA10_23420(mauB) PCA10_34250(mauB)
PCQ: PcP3B5_30170(aauB)
PPG: PputGB1_2224
PEN: PSEEN2552
PKC: PKB_2659(mauB)
PSET: THL1_3122 THL1_3147
PAT: Patl_0160
GAI: IMCC3135_19715(aauB)
AXE: P40_02495
BCN: Bcen_3305
BCJ: BCAM2304
BCEO: I35_6195
BAM: Bamb_4472
BMU: Bmul_3580
BMK: DM80_4965
BMUL: NP80_4961
BCT: GEM_3380
BCED: DM42_5686
BDL: AK34_3489
BCON: NL30_01045
BTEI: WS51_08600
BSEM: WJ12_31410
BPSL: WS57_02710
BMEC: WJ16_29850
BSTG: WT74_30220
BPH: Bphy_3809
AXX: ERS451415_01935(aauB)
AMIM: MIM_c29770(mauB)
MFA: Mfla_0548
MMB: Mmol_1575
AZO: azo2260(maub)
AOA: dqs_2391
RBS: RHODOSMS8_03495(aauB)
MEA: Mex_1p2770(mauB)
MCH: Mchl_0565
METD: C0214_27040(mauB)
PHL: KKY_2939
FIL: BN1229_v1_3765(mauB)
FIY: BN1229_v1_3755(mauB)
PLEO: OHA_1_02008(MadA)
CAK: Caul_0557
PDE: Pden_4730
PYE: A6J80_21995(mauB)
PKD: F8A10_20035(mauB)
CID: P73_3338
HBA: Hbal_2741
SMAZ: LH19_18390
SPHD: HY78_09285
SPHM: G432_07325
SSAN: NX02_15555
SPHR: BSY17_2813
GDI: GDI0271(mauB) GDI1806(mauB)
APK: APA386B_1936(mauB)
ASZ: ASN_2153(mauB)
MIN: Minf_1997
MKC: kam1_320
 » show all
Reference
PMID:8021187
  Authors
Chistoserdov AY, Chistoserdova LV, McIntire WS, Lidstrom ME
  Title
Genetic organization of the mau gene cluster in Methylobacterium extorquens AM1: complete nucleotide sequence and generation and characteristics of mau mutants.
  Journal
J Bacteriol 176:4052-65 (1994)
DOI:10.1128/jb.176.13.4052-4065.1994
  Sequence

KEGG   ENZYME: 1.4.9.1
Entry
EC 1.4.9.1                  Enzyme                                 

Name
methylamine dehydrogenase (amicyanin);
amine dehydrogenase;
primary-amine dehydrogenase;
amine: (acceptor) oxidoreductase (deaminating);
primary-amine:(acceptor) oxidoreductase (deaminating)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a copper protein as acceptor
Sysname
methylamine:amicyanin oxidoreductase (deaminating)
Reaction(IUBMB)
methylamine + H2O + 2 amicyanin = formaldehyde + NH3 + 2 reduced amicyanin [RN:R00606]
Reaction(KEGG)
R00606
Substrate
methylamine [CPD:C00218];
H2O [CPD:C00001];
amicyanin [CPD:C19671]
Product
formaldehyde [CPD:C00067];
NH3 [CPD:C00014];
reduced amicyanin [CPD:C19672]
Comment
Contains tryptophan tryptophylquinone (TTQ) cofactor. The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines.
History
EC 1.4.9.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, transferred 2011 to EC 1.4.9.1
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08685  quinohemoprotein amine dehydrogenase
K15228  methylamine dehydrogenase light chain
K15229  methylamine dehydrogenase heavy chain
Genes
YMO: HRD69_07500 HRD69_07515
VFU: vfu_A02015 vfu_A02018
VFL: AL536_13995 AL536_14010
PMK: MDS_4213 MDS_4216
PRE: PCA10_23420(mauB) PCA10_23450(mauA) PCA10_32510(peaA) PCA10_34250(mauB) PCA10_34280(mauA)
PCQ: PcP3B5_29870 PcP3B5_30140(aauA) PcP3B5_30170(aauB)
PPU: PP_5602(peaA)
PPF: Pput_2307
PPG: PputGB1_2221 PputGB1_2224 PputGB1_2475
PPW: PputW619_2945
PPT: PPS_2940
PPI: YSA_11298
PPX: T1E_1613
PPUH: B479_14560
PPUT: L483_18160
PPUN: PP4_23960(peaA)
PPUD: DW66_3200
PMON: X969_14050
PMOT: X970_13695
PFL: PFL_4120(peaA)
PPRO: PPC_4221(peaA)
PMAN: OU5_1439
PKC: PKB_2656(mauA) PKB_2659(mauB) PKB_2912
PSEM: TO66_21570
PSOS: POS17_4220(peaA)
PSIL: PMA3_20245
PKE: DLD99_19475(peaA)
HVN: EI420_18585(peaA)
NCU: F0U83_05970(peaA) F0U83_06020(peaA)
PSE: NH8B_2195
BCEN: DM39_4450(peaA)
BMK: DM80_4965 DM80_4968(aauA)
BMUL: NP80_4958(aauA) NP80_4961
BCED: DM42_5686 DM42_5689(aauA)
BDL: AK34_3489 AK34_3492(aauA)
AMIM: MIM_c29740(mauA) MIM_c29770(mauB)
HPSE: HPF_21410
MPT: Mpe_A2653
EBA: ebA2235(qhpA) ebA5178(qhpA)
AZO: azo1239(qhpA) azo2257(mauA) azo2260(maub)
AZA: AZKH_3023(qhpA)
AZD: CDA09_16050(peaA)
AZQ: G3580_00160(peaA)
TCL: Tchl_2123
ABU: Abu_0465
ABT: ABED_0440
AELL: AELL_0619
ASUI: ASUIS_0583
AMOL: AMOL_1804
ARC: ABLL_0607
DTO: TOL2_C06430(qhpA)
MEA: Mex_1p2770(mauB) Mex_1p2773(mauA)
METD: C0214_27025(mauA) C0214_27040(mauB)
FIL: BN1229_v1_3765(mauB) BN1229_v1_3768(mauA)
FIY: BN1229_v1_3755(mauB) BN1229_v1_3758(mauA)
BLAG: BLTE_16570
PLEO: OHA_1_02005(MadB) OHA_1_02008(MadA)
PYE: A6J80_01745(peaA) A6J80_21995(mauB) A6J80_22010(mauA)
PZH: CX676_03775(peaA)
PARO: CUV01_13130(peaA)
PKD: F8A10_18985(peaA) F8A10_20020(mauA) F8A10_20035(mauB)
PPAN: ESD82_08405(peaA)
LEJ: ETW24_23980(peaA)
LAQU: R2C4_20365(peaA)
RHC: RGUI_4107
LABR: CHH27_09745(peaA)
LABP: FJ695_27525(peaA)
THAS: C6Y53_00400(peaA)
SPHR: BSY17_2364(peaA) BSY17_2810(mauA) BSY17_2813
ALB: AEB_P3055
GDI: GDI0271(mauB) GDI0273(mauA) GDI1806(mauB) GDI1807(mauA)
APK: APA386B_1936(mauB) APA386B_1939(mauA)
ASZ: ASN_2153(mauB) ASN_2156(mauA)
MAGX: XM1_2531
AZZ: DEW08_00570(peaA)
ECOG: FIV45_14145(peaA) FIV45_16005(peaA)
BACO: OXB_3506
NMK: CHR53_12040(peaA)
CPRE: Csp1_26450(mauA)
RER: RER_53630
REY: O5Y_25460
REQ: REQ_44990(mauA)
RHS: A3Q41_02066(mauA)
RRT: 4535765_00133(mauA)
RCR: NCTC10994_01857(mauA)
TALB: FTW19_10610(peaA)
 » show all
Reference
1  [PMID:6246962]
  Authors
de Beer R, Duine JA, Frank J, Large PJ.
  Title
The prosthetic group of methylamine dehydrogenase from Pseudomonas AM1: evidence for a quinone structure.
  Journal
Biochim Biophys Acta 622:370-4 (1980)
DOI:10.1016/0005-2795(80)90050-1
Reference
2  [PMID:4388687]
  Authors
Eady RR, Large PJ.
  Title
Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine.
  Journal
Biochem J 106:245-55 (1968)
DOI:10.1042/bj1060245
Reference
3  [PMID:5124384]
  Authors
Eady RR, Large PJ.
  Title
Microbial oxidation of amines. Spectral and kinetic properties of the primary amine dehydrogenase of Pseudomonas AM1.
  Journal
Biochem J 123:757-71 (1971)
DOI:10.1042/bj1230757
Reference
4  [PMID:18512962]
  Authors
Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M
  Title
Structural comparison of crystal and solution states of the 138 kDa complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus.
  Journal
Biochemistry 47:6560-70 (2008)
DOI:10.1021/bi7023749
Reference
5  [PMID:20873742]
  Authors
Meschi F, Wiertz F, Klauss L, Cavalieri C, Blok A, Ludwig B, Heering HA, Merli A, Rossi GL, Ubbink M
  Title
Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex.
  Journal
J Am Chem Soc 132:14537-45 (2010)
DOI:10.1021/ja105498m
Other DBs
ExplorEnz - The Enzyme Database: 1.4.9.1
IUBMB Enzyme Nomenclature: 1.4.9.1
ExPASy - ENZYME nomenclature database: 1.4.9.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.4.9.1
BRENDA, the Enzyme Database: 1.4.9.1

KEGG   REACTION: R00606
Entry
R00606                      Reaction                               

Name
methylamine:amicyanin oxidoreductase (deaminating)
Definition
Methylamine + H2O + 2 Amicyanin <=> Formaldehyde + Ammonia + 2 Reduced amicyanin + 2 H+
Equation
Reaction class
RC00189  C00067_C00218
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K08685  quinohemoprotein amine dehydrogenase [EC:1.4.9.1]
K15228  methylamine dehydrogenase light chain [EC:1.4.9.1]
K15229  methylamine dehydrogenase heavy chain [EC:1.4.9.1]
Other DBs
RHEA: 30210

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