KEGG   ORTHOLOGY: K08691Help
Entry
K08691                      KO                                     

Name
mcl
Definition
malyl-CoA/(S)-citramalyl-CoA lyase [EC:4.1.3.24 4.1.3.25]
Pathway
ko00630  Glyoxylate and dicarboxylate metabolism
ko00660  C5-Branched dibasic acid metabolism
ko00680  Methane metabolism
ko00720  Carbon fixation pathways in prokaryotes
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00346  Formaldehyde assimilation, serine pathway
M00373  Ethylmalonyl pathway
M00376  3-Hydroxypropionate bi-cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
   00660 C5-Branched dibasic acid metabolism
    K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
  09102 Energy metabolism
   00720 Carbon fixation pathways in prokaryotes
    K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
   00680 Methane metabolism
    K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.3  Oxo-acid-lyases
    4.1.3.24  malyl-CoA lyase
     K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
    4.1.3.25  (S)-citramalyl-CoA lyase
     K08691  mcl; malyl-CoA/(S)-citramalyl-CoA lyase
BRITE hierarchy
Other DBs
RN: R00237 R00473 R00934
COG: COG2301
GO: 0050083 0047777
Genes
MCA: MCA1739
MMAI: sS8_2223
BLEP: AL038_08990
RPJ: N234_12055
CNC: CNE_BB1p10140(mcl)
BGP: BGL_2c16890
BPLA: bpln_2g17560
BPH: Bphy_5924
BFN: OI25_5771 OI25_7353
BCAI: K788_0001579
CBAB: SMCB_1586
MPT: Mpe_A3254
METR: BSY238_253
PLA: Plav_3098
RBS: RHODOSMS8_01011(mcl)
BRAD: BF49_2901
XAU: Xaut_3870
AZC: AZC_1227
MDI: METDI2485(mcl) METDI4906
MAQU: Maq22A_c18385(citE) Maq22A_c19250(mcl)
MSL: Msil_1719
MTUN: MTUNDRAET4_3402(mcl)
HDN: Hden_1659
RVA: Rvan_1682
BVR: BVIR_1803
BLAG: BLTE_09690(mclA)
PLEO: OHA_1_00777(citE)
MCG: GL4_3371
HDI: HDIA_2467(mcl1_2)
RBM: TEF_05695
CCR: CC_2999
CAK: Caul_0731
CSE: Cseg_3490
SIL: SPO1565 SPO3608(mclA)
RSP: RSP_1771
RCP: RCAP_rcc03164(citE2)
JAN: Jann_0471
RDE: RD1_1001(citE) RD1_1160(mclA) RD1_4242(mclA)
RLI: RLO149_c002070(mcl1) RLO149_c036850(mcl2)
PDE: Pden_0799
DSH: Dshi_3050(citE2)
PSF: PSE_3192
PGD: Gal_00369
OTM: OSB_28710(mcl1)
PTP: RCA23_c05980(mclA)
CID: P73_4243
MALG: MALG_03008
RSU: NHU_02809
RHC: RGUI_3145
SPSE: SULPSESMR1_03496(mcl1)
RMM: ROSMUCSMR3_02149(mcl1)
LVS: LOKVESSMR4R_02838(mcl1)
AHT: ANTHELSMS3_00280(mcl1)
HNE: HNE_0078(mclA)
HBA: Hbal_0778
RRU: Rru_A0217
RRF: F11_01090
MAG: amb3977
MGRY: MSR1_29830(mcl1)
MAGX: XM1_1373
MAGN: WV31_14965
TMO: TMO_0350(citE)
TXI: TH3_02680
MAGQ: MGMAQ_0659
SCT: SCAT_4364
SALU: DC74_4078
SALL: SAZ_21750
STRE: GZL_04819
SLD: T261_4311
NCA: Noca_4393
NDK: I601_0974(mcl1)
PSIM: KR76_23920
AMD: AMED_0079(citE)
AMN: RAM_00395
AMM: AMES_0076(citE)
AMZ: B737_0077(citE)
AOI: AORI_0068(mcl)
AMQ: AMETH_0090(citE)
KAL: KALB_2473
ALO: CRK61727
CWO: Cwoe_4213
RCA: Rcas_0912
CAU: Caur_0174
CAG: Cagg_1328
 » show all
TaxonomyKoalaUniProt
Reference
PMID:6094488
  Authors
Fulton GL, Nunn DN, Lidstrom ME
  Title
Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph.
  Journal
J Bacteriol 160:718-23 (1984)
Reference
  Authors
Friedmann S, Alber BE, Fuchs G
  Title
Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.
  Journal
J Bacteriol 189:2906-14 (2007)
DOI:10.1128/JB.01620-06
Reference
  Authors
Herter S, Busch A, Fuchs G
  Title
L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation.
  Journal
J Bacteriol 184:5999-6006 (2002)
DOI:10.1128/JB.184.21.5999-6006.2002
  Sequence
[cau:Caur_0174]

KEGG   ORTHOLOGY: K19281Help
Entry
K19281                      KO                                     

Name
mcl
Definition
beta-methylmalyl-CoA/(S)-malyl-CoA lyase [EC:4.1.3.24]
Pathway
ko00630  Glyoxylate and dicarboxylate metabolism
ko00660  C5-Branched dibasic acid metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00740  Methylaspartate cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K19281  mcl; beta-methylmalyl-CoA/(S)-malyl-CoA lyase
   00660 C5-Branched dibasic acid metabolism
    K19281  mcl; beta-methylmalyl-CoA/(S)-malyl-CoA lyase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.3  Oxo-acid-lyases
    4.1.3.24  malyl-CoA lyase
     K19281  mcl; beta-methylmalyl-CoA/(S)-malyl-CoA lyase
BRITE hierarchy
Other DBs
RN: R00934
COG: COG2301
GO: 0050083
Genes
HMA: rrnAC0690(citE1)
HHI: HAH_1341(citE1)
HHN: HISP_06865
HAB: SG26_14710
HTA: BVU17_06535
HMU: Hmuk_2984
HALZ: E5139_02195
HTU: Htur_1590
HDA: BB347_04010
HJT: DVR14_02935
NMG: Nmag_3338(mcl)
NAT: NJ7G_3289
SALI: L593_04660
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Khomyakova M, Bukmez O, Thomas LK, Erb TJ, Berg IA
  Title
A methylaspartate cycle in haloarchaea.
  Journal
Science 331:334-7 (2011)
DOI:10.1126/science.1196544
  Sequence
[hma:rrnAC0690]

KEGG   ENZYME: 4.1.3.24Help
Entry
EC 4.1.3.24                 Enzyme                                 

Name
malyl-CoA lyase;
malyl-coenzyme A lyase;
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase;
mclA (gene name);
mcl1 (gene name);
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming);
L-malyl-CoA lyase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
(S)-malyl-CoA glyoxylate-lyase (acetyl-CoA-forming)
Reaction(IUBMB)
(1) (S)-malyl-CoA = acetyl-CoA + glyoxylate [RN:R00473];
(2) (2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate [RN:R00934]
Reaction(KEGG)
Substrate
(S)-malyl-CoA [CPD:C04348];
(2R,3S)-2-methylmalyl-CoA [CPD:C06027]
Product
acetyl-CoA [CPD:C00024];
glyoxylate [CPD:C00048];
propanoyl-CoA [CPD:C00100]
Comment
The enzymes from Rhodobacter species catalyse a step in the ethylmalonyl-CoA pathway for acetate assimilation [3,5]. The enzyme from halophilic bacteria participate in the methylaspartate cycle and catalyse the reaction in the direction of malyl-CoA formation [6]. The enzyme from the bacterium Chloroflexus aurantiacus, which participates in the 3-hydroxypropanoate cycle for carbon assimilation, also has the activity of EC 4.1.3.25, (3S)-citramalyl-CoA lyase [2,4].
History
EC 4.1.3.24 created 1972, modified 2014
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec00660  C5-Branched dibasic acid metabolism
ec00680  Methane metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08691  malyl-CoA/(S)-citramalyl-CoA lyase
K19281  beta-methylmalyl-CoA/(S)-malyl-CoA lyase
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
MCA: MCA1739
MMAI: sS8_2223
BLEP: AL038_08990
RPJ: N234_12055
CNC: CNE_BB1p10140(mcl)
BGP: BGL_2c16890
BPLA: bpln_2g17560
BPH: Bphy_5924
BFN: OI25_5771 OI25_7353
BCAI: K788_0001579
CBAB: SMCB_1586
MPT: Mpe_A3254
METR: BSY238_253
PLA: Plav_3098
RBS: RHODOSMS8_01011(mcl)
BRAD: BF49_2901
XAU: Xaut_3870
AZC: AZC_1227
MDI: METDI2485(mcl) METDI4906
MAQU: Maq22A_c18385(citE) Maq22A_c19250(mcl)
MSL: Msil_1719
MTUN: MTUNDRAET4_3402(mcl)
HDN: Hden_1659
RVA: Rvan_1682
BVR: BVIR_1803
BLAG: BLTE_09690(mclA)
PLEO: OHA_1_00777(citE)
MCG: GL4_3371
HDI: HDIA_2467(mcl1_2)
RBM: TEF_05695
CCR: CC_2999
CAK: Caul_0731
CSE: Cseg_3490
SIL: SPO1565 SPO3608(mclA)
RSP: RSP_1771
RCP: RCAP_rcc03164(citE2)
JAN: Jann_0471
RDE: RD1_1001(citE) RD1_1160(mclA) RD1_4242(mclA)
RLI: RLO149_c002070(mcl1) RLO149_c036850(mcl2)
PDE: Pden_0799
DSH: Dshi_3050(citE2)
PSF: PSE_3192
PGD: Gal_00369
OTM: OSB_28710(mcl1)
PTP: RCA23_c05980(mclA)
CID: P73_4243
MALG: MALG_03008
RSU: NHU_02809
RHC: RGUI_3145
SPSE: SULPSESMR1_03496(mcl1)
RMM: ROSMUCSMR3_02149(mcl1)
LVS: LOKVESSMR4R_02838(mcl1)
AHT: ANTHELSMS3_00280(mcl1)
HNE: HNE_0078(mclA)
HBA: Hbal_0778
RRU: Rru_A0217
RRF: F11_01090
MAG: amb3977
MGRY: MSR1_29830(mcl1)
MAGX: XM1_1373
MAGN: WV31_14965
TMO: TMO_0350(citE)
TXI: TH3_02680
MAGQ: MGMAQ_0659
SCT: SCAT_4364
SALU: DC74_4078
SALL: SAZ_21750
STRE: GZL_04819
SLD: T261_4311
NCA: Noca_4393
NDK: I601_0974(mcl1)
PSIM: KR76_23920
AMD: AMED_0079(citE)
AMN: RAM_00395
AMM: AMES_0076(citE)
AMZ: B737_0077(citE)
AOI: AORI_0068(mcl)
AMQ: AMETH_0090(citE)
KAL: KALB_2473
ALO: CRK61727
CWO: Cwoe_4213
RCA: Rcas_0912
CAU: Caur_0174
CAG: Cagg_1328
HHB: Hhub_3380(aceB)
HSU: HLASF_2007(aceB)
HSF: HLASA_1993(aceB)
HMA: rrnAC0690(citE1) rrnAC1965(aceB)
HHI: HAH_1341(citE1) HAH_2476(aceB)
NPH: NP_4430A(aceB)
NMO: Nmlp_2357(aceB)
HMU: Hmuk_2984
HWA: HQ_1720A(aceB)
HWC: Hqrw_1842(aceB)
HVO: HVO_1983(aceB1) HVO_B0200(aceB2)
HME: HFX_2078(aceB)
NMG: Nmag_3338(mcl) Nmag_3363(aceB)
NAT: NJ7G_3289
SALI: L593_04660
 » show all
Taxonomy
Reference
1  [PMID:14285256]
  Authors
TUBOI S, KIKUCHI G.
  Title
ENZYMIC CLEAVAGE OF MALYTL-COENZYME A INTO ACETYL-COENZYME A AND GLYOXYLIC ACID.
  Journal
Biochim Biophys Acta 96:148-53 (1965)
DOI:10.1016/0005-2787(65)90618-0
Reference
2  [PMID:12374834]
  Authors
Herter S, Busch A, Fuchs G.
  Title
L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation.
  Journal
J Bacteriol 184:5999-6006 (2002)
DOI:10.1128/JB.184.21.5999-6006.2002
  Sequence
[cau:Caur_0174]
Reference
3  [PMID:15687206]
  Authors
Meister M, Saum S, Alber BE, Fuchs G
  Title
L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus.
  Journal
J Bacteriol 187:1415-25 (2005)
DOI:10.1128/JB.187.4.1415-1425.2005
  Sequence
Reference
4  [PMID:17259315]
  Authors
Friedmann S, Alber BE, Fuchs G
  Title
Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.
  Journal
J Bacteriol 189:2906-14 (2007)
DOI:10.1128/JB.01620-06
Reference
5  [PMID:20047909]
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and  (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_1771]
Reference
6  [PMID:27920298]
  Authors
Borjian F, Han J, Hou J, Xiang H, Zarzycki J, Berg IA
  Title
Malate Synthase and beta-Methylmalyl Coenzyme A Lyase Reactions in the Methylaspartate Cycle in Haloarcula hispanica.
  Journal
J Bacteriol 199 (2017)
DOI:10.1128/JB.00657-16
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.24
IUBMB Enzyme Nomenclature: 4.1.3.24
ExPASy - ENZYME nomenclature database: 4.1.3.24
BRENDA, the Enzyme Database: 4.1.3.24
CAS: 37290-67-8

KEGG   REACTION: R00934Help
Entry
R00934                      Reaction                               

Name
L-erythro-3-methylmalyl-CoA glyoxylate-lyase (propanoyl-CoA-forming)
Definition
Propanoyl-CoA + Glyoxylate <=> L-erythro-3-Methylmalyl-CoA
Equation
Reaction class
RC00311  C00048_C06027
RC00407  C00100_C06027
Enzyme
Pathway
rn00630  Glyoxylate and dicarboxylate metabolism
rn00660  C5-Branched dibasic acid metabolism
rn00720  Carbon fixation pathways in prokaryotes
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00373  Ethylmalonyl pathway
M00376  3-Hydroxypropionate bi-cycle
M00740  Methylaspartate cycle
Orthology
K08691  malyl-CoA/(S)-citramalyl-CoA lyase [EC:4.1.3.24 4.1.3.25]
K19281  beta-methylmalyl-CoA/(S)-malyl-CoA lyase [EC:4.1.3.24]
Other DBs
RHEA: 38262

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