KEGG   ORTHOLOGY: K10256
Entry
K10256                      KO                                     

Name
FAD2
Definition
omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase) [EC:1.14.19.6 1.14.19.22]
Pathway
ko01040  Biosynthesis of unsaturated fatty acids
ko01100  Metabolic pathways
ko01212  Fatty acid metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   01040 Biosynthesis of unsaturated fatty acids
    K10256  FAD2; omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K10256  FAD2; omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.6  acyl-CoA (9+3)-desaturase
     K10256  FAD2; omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
    1.14.19.22  acyl-lipid omega-6 desaturase (cytochrome b5)
     K10256  FAD2; omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Lipid biosynthesis proteins [BR:ko01004]
 Desaturase
  Omega
   K10256  FAD2; omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
Other DBs
RN: R11043 R11044
GO: 0045485
Genes
BTAB: 109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
NVE: 5512103
EPA: 110243700
ADF: 107344154
AMIL: 114948134 114973367
PDAM: 113667909 113682750
SPIS: 111329485 111342409
ATH: AT3G12120(FAD2)
ALY: 9318834
CRB: 17890721
BRP: 103870276
BOE: 106295108
RSZ: 108862913
CPAP: 110811677
CIT: 102627239
GMX: 100780912(GMFAD2-2D) 100805040(GMFAD2-2B) 100805777(FAD2-1B) 100809290(GMFAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
LJA: Lj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ZJU: 107432351
CSV: 101222623
CMO: 103486770
MCHA: 111024845
RCU: 8267086
JCU: 105646514
POP: 7479530
VVI: 100252129
SIND: 105159686 105163532(FAD2)
OSA: 4330523
DOSA: Os02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700)
OBR: 102719739
ATS: 109756354(LOC109756354) 109768827(LOC109768827) 109774922(LOC109774922) 109774938(LOC109774938) 109781985(LOC109781985)
ZMA: 100282744
PDA: 103713931
EGU: 105050201
MUS: 103980416
ATR: 18439780
MNG: MNEG_7806
ERC: Ecym_2769
TDL: TDEL_0E01510(TDEL0E01510)
PIC: PICST_72518(ODD1)
CAL: CAALFM_C601110WA(FAD2)
SLB: AWJ20_1033(ODE1)
NCR: NCU02209(ods)
NTE: NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
MGR: MGG_01985
SSCK: SPSK_00128
MAW: MAC_06628
MAJ: MAA_07889
CMT: CCM_06866
ANI: AN1037.2
ANG: ANI_1_742074(An08g05160)
ABE: ARB_01637
TVE: TRV_05152
PTE: PTT_13901
CNE: CND02750
CNB: CNBD3600
LBC: LACBIDRAFT_292603(FAD12-5)
ABP: AGABI1DRAFT113988(AGABI1DRAFT_113988)
ABV: AGABI2DRAFT193825(AGABI2DRAFT_193825)
MGL: MGL_1345
MRT: MRET_4015
NGD: NGA_0081700(DES)
TCR: 429257.20
 » show all
Reference
PMID:7907506
  Authors
Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, Browse J
  Title
Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis.
  Journal
Plant Cell 6:147-58 (1994)
DOI:10.1105/tpc.6.1.147
  Sequence
[ath:AT3G12120]
Reference
  Authors
Petrini GA, Altabe SG, Uttaro AD
  Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
  Journal
Eur J Biochem 271:1079-86 (2004)
DOI:10.1111/j.1432-1033.2004.04005.x
  Sequence
Reference
  Authors
Kainou K, Kamisaka Y, Kimura K, Uemura H
  Title
Isolation of Delta12 and omega3-fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and alpha-linolenic acids in Saccharomyces cerevisiae.
  Journal
Yeast 23:605-12 (2006)
DOI:10.1002/yea.1378
  Sequence

KEGG   ENZYME: 1.14.19.6
Entry
EC 1.14.19.6                Enzyme                                 

Name
acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11043];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11044]
Reaction(KEGG)
R11043 R11044
Substrate
oleoyl-CoA [CPD:C00510];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-CoA [CPD:C21072]
Product
linoleoyl-CoA [CPD:C02050];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:C21000]
Comment
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
History
EC 1.14.19.6 created 2008, modified 2015
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
K22993  bifunctional Delta-12/omega-3 fatty acid desaturase
Genes
BTAB: 109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
NVE: 5512103
EPA: 110243700
ADF: 107344154
AMIL: 114948134 114973367
PDAM: 113667909 113682750
SPIS: 111329485 111342409
ATH: AT3G12120(FAD2)
ALY: 9318834
CRB: 17890721
BRP: 103870276
BOE: 106295108
RSZ: 108862913
CPAP: 110811677
CIT: 102627239
GMX: 100780912(GMFAD2-2D) 100805040(GMFAD2-2B) 100805777(FAD2-1B) 100809290(GMFAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
LJA: Lj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ZJU: 107432351
CSV: 101222623
CMO: 103486770
MCHA: 111024845
RCU: 8267086
JCU: 105646514
POP: 7479530
VVI: 100252129
SIND: 105159686 105163532(FAD2)
OSA: 4330523
DOSA: Os02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700)
OBR: 102719739
ATS: 109756354(LOC109756354) 109768827(LOC109768827) 109774922(LOC109774922) 109774938(LOC109774938) 109781985(LOC109781985)
ZMA: 100282744
PDA: 103713931
EGU: 105050201
MUS: 103980416
ATR: 18439780
MNG: MNEG_7806
ERC: Ecym_2769
TDL: TDEL_0E01510(TDEL0E01510)
PIC: PICST_72518(ODD1)
CAL: CAALFM_C601110WA(FAD2)
SLB: AWJ20_1033(ODE1)
NCR: NCU02209(ods) NCU09497
NTE: NEUTE1DRAFT116751(NEUTE1DRAFT_116751) NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
MAW: MAC_06628
MAJ: MAA_07889
ANG: ANI_1_3412014(An01g14970) ANI_1_742074(An08g05160) ANI_1_962124(An14g06980)
ABE: ARB_01637
TVE: TRV_05152
PTE: PTT_13901
CNE: CND02750
CNB: CNBD3600
LBC: LACBIDRAFT_292603(FAD12-5)
ABP: AGABI1DRAFT113988(AGABI1DRAFT_113988)
ABV: AGABI2DRAFT193825(AGABI2DRAFT_193825)
MGL: MGL_1345
MRT: MRET_4015
NGD: NGA_0081700(DES)
TCR: 429257.20
 » show all
Reference
1  [PMID:2248971]
  Authors
Borgeson CE, de Renobales M, Blomquist GJ.
  Title
Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.
  Journal
Biochim Biophys Acta 1047:135-40 (1990)
DOI:10.1016/0005-2760(90)90039-Z
Reference
2  [PMID:8900454]
  Authors
Lomascolo A, Dubreucq E, Galzy P.
  Title
Study of the delta 12-desaturase system of Lipomyces starkeyi.
  Journal
Lipids 31:253-9 (1996)
DOI:10.1007/BF02529871
Reference
3  [PMID:9829122]
  Authors
Tocher DR, Leaver MJ, Hodgson PA.
  Title
Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.
  Journal
Prog Lipid Res 37:73-117 (1998)
DOI:10.1016/S0163-7827(98)00005-8
Reference
4  [PMID:15009186]
  Authors
Petrini GA, Altabe SG, Uttaro AD
  Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
  Journal
Eur J Biochem 271:1079-86 (2004)
DOI:10.1111/j.1432-1033.2004.04005.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.6
IUBMB Enzyme Nomenclature: 1.14.19.6
ExPASy - ENZYME nomenclature database: 1.14.19.6
BRENDA, the Enzyme Database: 1.14.19.6

KEGG   REACTION: R11043
Entry
R11043                      Reaction                               

Name
oleoyl-CoA,ferrocytochrome-b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Definition
Oleoyl-CoA + 2 Ferrocytochrome b5 + Oxygen + 2 H+ <=> Linoleoyl-CoA + 2 Ferricytochrome b5 + 2 H2O
Equation
C00510 + 2 C00999 + C00007 + 2 C00080 <=> C02050 + 2 C00996 + 2 C00001
Reaction class
RC00917  C00510_C02050
Enzyme
Pathway
rn01040  Biosynthesis of unsaturated fatty acids
rn01212  Fatty acid metabolism
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase) [EC:1.14.19.6 1.14.19.22]
Other DBs
RHEA: 25859

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