KEGG   ORTHOLOGY: K10619
Entry
K10619                      KO                                     

Name
cmtAb
Definition
p-cumate 2,3-dioxygenase subunit alpha [EC:1.14.12.25]
Pathway
ko00622  Xylene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K10619  cmtAb; p-cumate 2,3-dioxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.25  p-cumate 2,3-dioxygenase
     K10619  cmtAb; p-cumate 2,3-dioxygenase subunit alpha
Other DBs
RN: R05247
COG: COG4638
GO: 0018570
Genes
RBD: ALSL_1087
PAE: PA2083
PAEV: N297_2148
PAEI: N296_2148
PAU: PA14_37570
PAG: PLES_32381
PAF: PAM18_2960
PNC: NCGM2_3063
PAEB: NCGM1900_4398
PDK: PADK2_15235
PAEP: PA1S_15265
PAEM: U769_14865
PAEL: T223_16545
PAEG: AI22_18505
PAEC: M802_2145
PAEO: M801_2147
PPF: Pput_2898
PPX: T1E_4248(cbdC)
PMON: X969_10845
PMOT: X970_10500
PSES: PSCI_1388
BXE: Bxe_A3556
BXB: DR64_1255
SMAZ: LH19_27420
SWI: Swit_1758
SPHD: HY78_16795
SECH: B18_22315
MHAS: MHAS_04026(cbdA_2)
ROP: ROP_46340(cmtA1)
RRT: 4535765_00187(cmtA1_1) 4535765_00375(cmtA1_2)
SALS: SLNWT_6907
PSIM: KR76_24915
AMQ: AMETH_1199(benA)
PDX: Psed_4287
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2898]

KEGG   ORTHOLOGY: K16303
Entry
K16303                      KO                                     

Name
cmtAc
Definition
p-cumate 2,3-dioxygenase subunit beta [EC:1.14.12.25]
Pathway
ko00622  Xylene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K16303  cmtAc; p-cumate 2,3-dioxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.25  p-cumate 2,3-dioxygenase
     K16303  cmtAc; p-cumate 2,3-dioxygenase subunit beta
Other DBs
RN: R05247
GO: 0018570
Genes
RBD: ALSL_1088
PAE: PA2085
PAEV: N297_2150
PAEI: N296_2150
PAU: PA14_37550
PAG: PLES_32361
PAF: PAM18_2958
PNC: NCGM2_3065
PAEB: NCGM1900_4396
PDK: PADK2_15225
PAEP: PA1S_15255
PAEM: U769_14855
PAEL: T223_16535
PAEG: AI22_18515
PAEC: M802_2147
PAEO: M801_2149
PPF: Pput_2897
PPX: T1E_4249
PMON: X969_10850
PMOT: X970_10505
PSES: PSCI_1386
BXE: Bxe_A3555
BXB: DR64_1254
SMAZ: LH19_27425
SWI: Swit_1757
SPHD: HY78_16800
SECH: B18_22325
MHAS: MHAS_04025(bnzB)
ROP: ROP_46330(cmtA2)
RRT: 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2)
SALS: SLNWT_6906
PSIM: KR76_24910
PDX: Psed_4289
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2897]

KEGG   ORTHOLOGY: K16304
Entry
K16304                      KO                                     

Name
cmtAd
Definition
p-cumate 2,3-dioxygenase ferredoxin component
Pathway
ko00622  Xylene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K16304  cmtAd; p-cumate 2,3-dioxygenase ferredoxin component
Other DBs
RN: R05247
Genes
PPF: Pput_2894
PPX: T1E_4252
PMON: X969_10865
PMOT: X970_10520
SPOI: IMCC21906_02717
BXE: Bxe_A3552
BXB: DR64_1251(bphA3)
BRK: CWS35_25080
NOR: FA702_19655
SWI: Swit_0969
SPHD: HY78_21670
ROP: ROP_46320(cmtA3)
SALS: SLNWT_6905
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2894]

KEGG   ORTHOLOGY: K18227
Entry
K18227                      KO                                     

Name
cmtAa
Definition
p-cumate 2,3-dioxygenase ferredoxin reductase component [EC:1.18.1.3]
Pathway
ko00622  Xylene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K18227  cmtAa; p-cumate 2,3-dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K18227  cmtAa; p-cumate 2,3-dioxygenase ferredoxin reductase component
Other DBs
RN: R05247
Genes
PPF: Pput_2899
PPX: T1E_4247(camA)
PMON: X969_10840
PMOT: X970_10495
BXE: Bxe_A3557
BXB: DR64_1256(bphA4)
MON: G8E03_15155
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2899]

KEGG   ENZYME: 1.14.12.25
Entry
EC 1.14.12.25               Enzyme                                 

Name
p-cumate 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
Reaction(IUBMB)
p-cumate + NADH + H+ + O2 = (2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+ [RN:R05247]
Reaction(KEGG)
R05247
Substrate
p-cumate [CPD:C06578];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate [CPD:C06579];
NAD+ [CPD:C00003]
Comment
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
History
EC 1.14.12.25 created 2016
Pathway
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10619  p-cumate 2,3-dioxygenase subunit alpha
K16303  p-cumate 2,3-dioxygenase subunit beta
Genes
RBD: ALSL_1087 ALSL_1088
PAE: PA2083 PA2085
PAEV: N297_2148 N297_2150
PAEI: N296_2148 N296_2150
PAU: PA14_37550 PA14_37570
PAG: PLES_32361 PLES_32381
PAF: PAM18_2958 PAM18_2960
PNC: NCGM2_3063 NCGM2_3065
PAEB: NCGM1900_4396 NCGM1900_4398
PDK: PADK2_15225 PADK2_15235
PPX: T1E_4248(cbdC) T1E_4249
MHAS: MHAS_04025(bnzB) MHAS_04026(cbdA_2)
ROP: ROP_46330(cmtA2) ROP_46340(cmtA1)
RRT: 4535765_00187(cmtA1_1) 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2) 4535765_00375(cmtA1_2)
 » show all
Reference
1  [PMID:845117]
  Authors
DeFrank JJ, Ribbons DW
  Title
p-cymene pathway in Pseudomonas putida: initial reactions.
  Journal
J Bacteriol 129:1356-64 (1977)
DOI:10.1128/JB.129.3.1356-1364.1977
Reference
2  [PMID:7204334]
  Authors
Wigmore GJ, Ribbons DW
  Title
p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs.
  Journal
J Bacteriol 143:816-24 (1980)
DOI:10.1128/JB.143.2.816-824.1980
Reference
3  [PMID:7592495]
  Authors
Eaton RW, Chapman PJ
  Title
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids.
  Journal
J Bacteriol 177:6983-8 (1995)
DOI:10.1128/JB.177.23.6983-6988.1995
Reference
4  [PMID:8631713]
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.25
IUBMB Enzyme Nomenclature: 1.14.12.25
ExPASy - ENZYME nomenclature database: 1.14.12.25
BRENDA, the Enzyme Database: 1.14.12.25

KEGG   REACTION: R05247
Entry
R05247                      Reaction                               

Name
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
Definition
p-Cumate + Oxygen + NADH + H+ <=> cis-2,3-Dihydroxy-2,3-dihydro-p-cumate + NAD+
Equation
Comment
p-cumate 2,3-dioxygenase
Reaction class
RC00001  C00003_C00004
RC00267  C06578_C06579
Enzyme
Pathway
rn00622  Xylene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Orthology
K10619  p-cumate 2,3-dioxygenase subunit alpha [EC:1.14.12.25]
K16303  p-cumate 2,3-dioxygenase subunit beta [EC:1.14.12.25]
K16304  p-cumate 2,3-dioxygenase ferredoxin component
K18227  p-cumate 2,3-dioxygenase ferredoxin reductase component [EC:1.18.1.3]
Other DBs
RHEA: 42347

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