KEGG   ORTHOLOGY: K10674
Entry
K10674                      KO                                     

Name
ectD
Definition
ectoine hydroxylase [EC:1.14.11.55]
Pathway
ko00260  Glycine, serine and threonine metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K10674  ectD; ectoine hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.55  ectoine hydroxylase
     K10674  ectD; ectoine hydroxylase
Other DBs
RN: R08050
Genes
PAO: Pat9b_4264
SALY: E8E00_13000
SKS: FCN78_00650
SCOT: HBA18_00655
PFS: PFLU_4269
PVR: PverR02_21760
PFF: PFLUOLIPICF717420
PSA: PST_0178
PSZ: PSTAB_0243
PSR: PSTAA_0241
PSTT: CH92_00965
PANR: A7J50_3931
PSED: DM292_17915(thpD)
PAT: Patl_1961
PART: PARC_a2189
PAGA: PAGA_a1714
MHC: MARHY1452(ectD) MARHY3849(ectD)
MAD: HP15_3697
MBS: MRBBS_1777(htxA)
AMAL: I607_20252
AMAE: I876_01340
AMAO: I634_01440
AMAI: I635_21544
HMI: soil367_13195(thpD) soil367_14180(thpD)
SDE: Sde_1259
MAH: MEALZ_3246(ectD)
CYQ: Q91_0890
CYY: CPC19_08285(thpD)
NOC: Noc_1560
NHL: Nhal_2706
NWA: Nwat_1573
NWR: E3U44_10635(thpD)
AEH: Mlg_0392
GAI: IMCC3135_09590(ectD_2)
HCH: HCH_01507
HAHE: ENC22_23015(thpD)
HEL: HELO_4008(ectD)
HHH: CLM76_02840(thpD)
HBE: BEI_0262(ectD1) BEI_2549(ectD2)
HAF: C8233_10080(thpD)
HALK: CUU95_09235(thpD)
HVN: EI420_16905(thpD)
HMD: CTT34_15390(thpD)
HAXI: HAALTHF_18370n(ectD)
KUY: FY550_02245(thpD)
ABO: ABO_0023
ADI: B5T_04304(ectD)
APAC: S7S_04430
AXE: P40_20965
NCU: F0U83_00805(thpD)
SALN: SALB1_2995
CHRB: DK843_11415(thpD)
CHRM: FYK34_14440(thpD)
CNC: CNE_BB2p03430(ectD)
BSTG: WT74_26330
BPAR: BN117_2970
BPA: BPP1891
BBR: BB3217
BPT: Bpet1984
BAV: BAV2371(thpD)
BHO: D560_0723(thpD)
BHM: D558_0712(thpD)
BTRM: SAMEA390648704056(thpD_2)
BOZ: DBV39_06675(thpD)
AXY: AXYL_02147(thpD)
ASW: CVS48_14530(thpD)
ACHR: C2U31_25480(thpD)
ACHB: DVB37_08455(thpD)
PUD: G9Q38_12440(thpD)
AMIM: MIM_c00990(ectD1) MIM_c17790(ectD2)
AFQ: AFA_12625(thpD)
PIG: EGT29_07190(thpD)
KGY: EHF36_15620(thpD)
POL: Bpro_1714
RTA: Rta_12710
HYC: E5678_08805(thpD)
HYN: F9K07_04935(thpD)
HAR: HEAR3377(ectD)
MMS: mma_3598
AHS: AHALO_1556(ectD)
AELL: AELL_2036(ectD)
AAQI: AAQM_0635(ectD)
ARC: ABLL_2145
PMOB: HG718_03635(thpD)
ABAW: D5400_10860(thpD)
BJA: blr7814
BRA: BRADO6432
BBT: BBta_1202
BRS: S23_63740
AOL: S58_65510
BRAD: BF49_3241
PHL: KKY_3694
DEI: C4375_19440(thpD)
YTI: FNA67_05985(thpD)
PZU: PHZ_c1338
RDE: RD1_0673
SAGU: CDO87_20350(thpD)
LIT: FPZ52_06940(thpD)
OCD: FHY55_00400(thpD)
HNE: HNE_1642
NOV: TQ38_019225(thpD)
SAL: Sala_2952
SPHK: SKP52_15805(ectD)
SPHP: LH20_15525
SGI: SGRAN_2348(ectD)
SPHO: C3E99_07240(thpD)
SPHX: E5675_00345(thpD)
SPHU: SPPYR_3379(ectD)
SPHI: TS85_04680
SSY: SLG_08070(ectD)
SPMI: K663_18946
SPHR: BSY17_1172(thpD)
SPHT: K426_26465
SHYD: CJD35_04525(thpD)
SYA: A6768_16850(thpD)
BLAS: BSY18_3438
SMIC: SmB9_21720
ALB: AEB_P3387
THAC: CSC3H3_05330(thpD)
NCB: C0V82_13055(thpD)
BCL: ABC3489
BPF: BpOF4_12395(ectD)
HHD: HBHAL_3406(ectD)
VIM: GWK91_05190(thpD)
GRC: GI584_06005(thpD)
RUE: DT065_11560(thpD)
SALE: EPH95_10530(thpD) EPH95_17035(thpD)
MMC: Mmcs_4193
MKM: Mkms_4259
MJL: Mjls_4420
MSM: MSMEG_3898(thpD)
MVA: Mvan_5271
MGI: Mflv_4835
MHAS: MHAS_00348(ectD)
MDU: MDUV_49230(thpD)
MCHT: MCHIJ_24920(thpD)
MAB: MAB_2264c
MABB: MASS_2187
MCHE: BB28_11340
MSTE: MSTE_02193
MMIN: MMIN_04010(ectD)
ASD: AS9A_2077(ectD)
MKR: MKOR_19740(thpD)
NFA: NFA_27190(ectD)
NCY: NOCYR_3186(ectD)
NNO: NONO_c56240(ectD)
NSR: NS506_03741(ectD)
NTP: CRH09_08825(thpD) CRH09_28080(thpD)
NOZ: DMB37_05120(thpD)
NOD: FOH10_10690(thpD)
ROP: ROP_44930(ectD) ROP_50010(ectD)
REQ: REQ_07850
RRZ: CS378_21165(thpD)
RBY: CEJ39_06835(thpD)
GOR: KTR9_4109
GOC: CXX93_02050(thpD)
GIT: C6V83_11400(thpD)
GOM: D7316_04338(ectD)
TPR: Tpau_3672
DIT: C3V38_09130(thpD)
TOY: FO059_13125(thpD)
SCO: SCO1867(SCI39.14)
SALB: XNR_4959
SMA: SAVERM_6395(ectD)
SGR: SGR_5632(ectD)
SGB: WQO_07155
SCB: SCAB_70711(ectD)
SCT: SCAT_1067(ectD)
SBH: SBI_06435 SBI_08130(ectD)
SVE: SVEN_0229
SALS: SLNWT_6006
SFI: SFUL_1437
SALU: DC74_2369
SALL: SAZ_13200
SLV: SLIV_28380(ectD)
SGU: SGLAU_08340(ectD)
STRE: GZL_06637
SAMB: SAM23877_1941(ectD)
SPRI: SPRI_5647
SLE: sle_52670(sle_52670)
SNR: SNOUR_30890(ectD)
STRD: NI25_29875
SMAL: SMALA_1826
SLAU: SLA_7411
SALF: SMD44_02016(ectD)
SALJ: SMD11_5132(ectD) SMD11_6819(ectD)
SNZ: DC008_07570(thpD)
SGE: DWG14_06535(ectD)
SLK: SLUN_09020(thpD)
SDX: C4B68_30980(thpD)
SALD: FVA74_05325(thpD)
AUL: DCC27_009910(thpD)
LMOI: VV02_20580
BLIN: BLSMQ_1388
KFL: Kfla_5740
NDA: Ndas_0138
NAL: B005_2939(thpD)
NGV: CDO52_05680(thpD)
ACTW: F7P10_37530(thpD)
SRO: Sros_5544
SEN: SACE_0486(ectD)
SACE: GIY23_02055(thpD)
SACG: FDZ84_14085(thpD)
AMD: AMED_8594(ectD)
AMN: RAM_44110
AMM: AMES_8463(ectD)
AMZ: B737_8464(ectD)
AOI: AORI_7380(ectD)
AJA: AJAP_02100(ectD)
AMQ: AMETH_6462(ectD)
AMYC: CU254_35655(thpD)
AMYY: YIM_03165
PDX: Psed_5823
PSEA: WY02_14010
PSEE: FRP1_28540
PAUT: Pdca_62800(ectD)
ACTI: UA75_01850
ACAD: UA74_01845
ACTA: C1701_09155(thpD)
SAQ: Sare_4561
ASE: ACPL_7613
AFS: AFR_38385
ACTS: ACWT_7483
SNA: Snas_5814
AEY: CDG81_13645(thpD)
CWO: Cwoe_3038
AVA: Ava_C0003
DOU: BMF77_01760(ectD)
RBA: RB7491
PSL: Psta_3174
PLS: VT03_03215(ptlH_1)
FMR: Fuma_00298(ptlH_1)
CPI: Cpin_5186
CHU: CHU_3622
NMR: Nmar_1343
AG: AAY29689(ectD)
 » show all
Reference
  Authors
Bursy J, Pierik AJ, Pica N, Bremer E.
  Title
Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase.
  Journal
J Biol Chem 282:31147-55 (2007)
DOI:10.1074/jbc.M704023200
  Sequence

KEGG   ENZYME: 1.14.11.55
Entry
EC 1.14.11.55               Enzyme                                 

Name
ectoine hydroxylase;
ectD (gene name);
ectoine dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
ectoine,2-oxoglutarate:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
ectoine + 2-oxoglutarate + O2 = 5-hydroxyectoine + succinate + CO2 [RN:R08050]
Reaction(KEGG)
R08050
Substrate
ectoine [CPD:C06231];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
5-hydroxyectoine [CPD:C16432];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme, found in bacteria, is specific for ectoine.
History
EC 1.14.11.55 created 2017
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K10674  ectoine hydroxylase
Genes
PAO: Pat9b_4264
SALY: E8E00_13000
SKS: FCN78_00650
SCOT: HBA18_00655
PFS: PFLU_4269
PVR: PverR02_21760
PFF: PFLUOLIPICF717420
PSA: PST_0178
PSZ: PSTAB_0243
PSR: PSTAA_0241
PSTT: CH92_00965
PANR: A7J50_3931
PSED: DM292_17915(thpD)
PAT: Patl_1961
PART: PARC_a2189
PAGA: PAGA_a1714
MHC: MARHY1452(ectD) MARHY3849(ectD)
MAD: HP15_3697
MBS: MRBBS_1777(htxA)
AMAL: I607_20252
AMAE: I876_01340
AMAO: I634_01440
AMAI: I635_21544
HMI: soil367_13195(thpD) soil367_14180(thpD)
SDE: Sde_1259
MAH: MEALZ_3246(ectD)
CYQ: Q91_0890
CYY: CPC19_08285(thpD)
NOC: Noc_1560
NHL: Nhal_2706
NWA: Nwat_1573
NWR: E3U44_10635(thpD)
AEH: Mlg_0392
GAI: IMCC3135_09590(ectD_2)
HCH: HCH_01507
HAHE: ENC22_23015(thpD)
HEL: HELO_4008(ectD)
HHH: CLM76_02840(thpD)
HBE: BEI_0262(ectD1) BEI_2549(ectD2)
HAF: C8233_10080(thpD)
HALK: CUU95_09235(thpD)
HVN: EI420_16905(thpD)
HMD: CTT34_15390(thpD)
HAXI: HAALTHF_18370n(ectD)
KUY: FY550_02245(thpD)
ABO: ABO_0023
ADI: B5T_04304(ectD)
APAC: S7S_04430
AXE: P40_20965
NCU: F0U83_00805(thpD)
SALN: SALB1_2995
CHRB: DK843_11415(thpD)
CHRM: FYK34_14440(thpD)
CNC: CNE_BB2p03430(ectD)
BSTG: WT74_26330
BPAR: BN117_2970
BPA: BPP1891
BBR: BB3217
BPT: Bpet1984
BAV: BAV2371(thpD)
BHO: D560_0723(thpD)
BHM: D558_0712(thpD)
BTRM: SAMEA390648704056(thpD_2)
BOZ: DBV39_06675(thpD)
AXY: AXYL_02147(thpD)
ASW: CVS48_14530(thpD)
ACHR: C2U31_25480(thpD)
ACHB: DVB37_08455(thpD)
PUD: G9Q38_12440(thpD)
AMIM: MIM_c00990(ectD1) MIM_c17790(ectD2)
AFQ: AFA_12625(thpD)
PIG: EGT29_07190(thpD)
KGY: EHF36_15620(thpD)
POL: Bpro_1714
RTA: Rta_12710
HYC: E5678_08805(thpD)
HYN: F9K07_04935(thpD)
HAR: HEAR3377(ectD)
MMS: mma_3598
AHS: AHALO_1556(ectD)
AELL: AELL_2036(ectD)
AAQI: AAQM_0635(ectD)
ARC: ABLL_2145
PMOB: HG718_03635(thpD)
ABAW: D5400_10860(thpD)
BJA: blr7814
BRA: BRADO6432
BBT: BBta_1202
BRS: S23_63740
AOL: S58_65510
BRAD: BF49_3241
PHL: KKY_3694
DEI: C4375_19440(thpD)
YTI: FNA67_05985(thpD)
PZU: PHZ_c1338
RDE: RD1_0673
SAGU: CDO87_20350(thpD)
LIT: FPZ52_06940(thpD)
OCD: FHY55_00400(thpD)
HNE: HNE_1642
NOV: TQ38_019225(thpD)
SAL: Sala_2952
SPHK: SKP52_15805(ectD)
SPHP: LH20_15525
SGI: SGRAN_2348(ectD)
SPHO: C3E99_07240(thpD)
SPHX: E5675_00345(thpD)
SPHU: SPPYR_3379(ectD)
SPHI: TS85_04680
SSY: SLG_08070(ectD)
SPMI: K663_18946
SPHR: BSY17_1172(thpD)
SPHT: K426_26465
SHYD: CJD35_04525(thpD)
SYA: A6768_16850(thpD)
BLAS: BSY18_3438
SMIC: SmB9_21720
ALB: AEB_P3387
THAC: CSC3H3_05330(thpD)
NCB: C0V82_13055(thpD)
BCL: ABC3489
BPF: BpOF4_12395(ectD)
HHD: HBHAL_3406(ectD)
VIM: GWK91_05190(thpD)
GRC: GI584_06005(thpD)
RUE: DT065_11560(thpD)
SALE: EPH95_10530(thpD) EPH95_17035(thpD)
MMC: Mmcs_4193
MKM: Mkms_4259
MJL: Mjls_4420
MSM: MSMEG_3898(thpD)
MVA: Mvan_5271
MGI: Mflv_4835
MHAS: MHAS_00348(ectD)
MDU: MDUV_49230(thpD)
MCHT: MCHIJ_24920(thpD)
MAB: MAB_2264c
MABB: MASS_2187
MCHE: BB28_11340
MSTE: MSTE_02193
MMIN: MMIN_04010(ectD)
ASD: AS9A_2077(ectD)
MKR: MKOR_19740(thpD)
NFA: NFA_27190(ectD)
NCY: NOCYR_3186(ectD)
NNO: NONO_c56240(ectD)
NSR: NS506_03741(ectD)
NTP: CRH09_08825(thpD) CRH09_28080(thpD)
NOZ: DMB37_05120(thpD)
NOD: FOH10_10690(thpD)
ROP: ROP_44930(ectD) ROP_50010(ectD)
REQ: REQ_07850
RRZ: CS378_21165(thpD)
RBY: CEJ39_06835(thpD)
GOR: KTR9_4109
GOC: CXX93_02050(thpD)
GIT: C6V83_11400(thpD)
GOM: D7316_04338(ectD)
TPR: Tpau_3672
DIT: C3V38_09130(thpD)
TOY: FO059_13125(thpD)
SCO: SCO1867(SCI39.14)
SALB: XNR_4959
SMA: SAVERM_6395(ectD)
SGR: SGR_5632(ectD)
SGB: WQO_07155
SCB: SCAB_70711(ectD)
SCT: SCAT_1067(ectD)
SBH: SBI_06435 SBI_08130(ectD)
SVE: SVEN_0229
SALS: SLNWT_6006
SFI: SFUL_1437
SALU: DC74_2369
SALL: SAZ_13200
SLV: SLIV_28380(ectD)
SGU: SGLAU_08340(ectD)
STRE: GZL_06637
SAMB: SAM23877_1941(ectD)
SPRI: SPRI_5647
SLE: sle_52670(sle_52670)
SNR: SNOUR_30890(ectD)
STRD: NI25_29875
SMAL: SMALA_1826
SLAU: SLA_7411
SALF: SMD44_02016(ectD)
SALJ: SMD11_5132(ectD) SMD11_6819(ectD)
SNZ: DC008_07570(thpD)
SGE: DWG14_06535(ectD)
SLK: SLUN_09020(thpD)
SDX: C4B68_30980(thpD)
SALD: FVA74_05325(thpD)
AUL: DCC27_009910(thpD)
LMOI: VV02_20580
BLIN: BLSMQ_1388
KFL: Kfla_5740
NDA: Ndas_0138
NAL: B005_2939(thpD)
NGV: CDO52_05680(thpD)
ACTW: F7P10_37530(thpD)
SRO: Sros_5544
SEN: SACE_0486(ectD)
SACE: GIY23_02055(thpD)
SACG: FDZ84_14085(thpD)
AMD: AMED_8594(ectD)
AMN: RAM_44110
AMM: AMES_8463(ectD)
AMZ: B737_8464(ectD)
AOI: AORI_7380(ectD)
AJA: AJAP_02100(ectD)
AMQ: AMETH_6462(ectD)
AMYC: CU254_35655(thpD)
AMYY: YIM_03165
PDX: Psed_5823
PSEA: WY02_14010
PSEE: FRP1_28540
PAUT: Pdca_62800(ectD)
ACTI: UA75_01850
ACAD: UA74_01845
ACTA: C1701_09155(thpD)
SAQ: Sare_4561
ASE: ACPL_7613
AFS: AFR_38385
ACTS: ACWT_7483
SNA: Snas_5814
AEY: CDG81_13645(thpD)
CWO: Cwoe_3038
AVA: Ava_C0003
DOU: BMF77_01760(ectD)
RBA: RB7491
PSL: Psta_3174
PLS: VT03_03215(ptlH_1)
FMR: Fuma_00298(ptlH_1)
CPI: Cpin_5186
CHU: CHU_3622
NMR: Nmar_1343
 » show all
Reference
1  [PMID:17636255]
  Authors
Bursy J, Pierik AJ, Pica N, Bremer E.
  Title
Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase.
  Journal
J Biol Chem 282:31147-55 (2007)
DOI:10.1074/jbc.M704023200
  Sequence
Reference
2  [PMID:18849444]
  Authors
Bursy J, Kuhlmann AU, Pittelkow M, Hartmann H, Jebbar M, Pierik AJ, Bremer E
  Title
Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses.
  Journal
Appl Environ Microbiol 74:7286-96 (2008)
DOI:10.1128/AEM.00768-08
  Sequence
[sco:SCO1867]
Reference
3  [PMID:20498719]
  Authors
Reuter K, Pittelkow M, Bursy J, Heine A, Craan T, Bremer E
  Title
Synthesis of 5-hydroxyectoine from ectoine: crystal structure of the non-heme iron(II) and 2-oxoglutarate-dependent dioxygenase EctD.
  Journal
PLoS One 5:e10647 (2010)
DOI:10.1371/journal.pone.0010647
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.55
IUBMB Enzyme Nomenclature: 1.14.11.55
ExPASy - ENZYME nomenclature database: 1.14.11.55
BRENDA, the Enzyme Database: 1.14.11.55

KEGG   REACTION: R08050
Entry
R08050                      Reaction                               

Definition
Ectoine + 2-Oxoglutarate + Oxygen <=> 5-Hydroxyectoine + Succinate + CO2
Equation
Reaction class
RC00661  C06231_C16432
Enzyme
Pathway
rn00260  Glycine, serine and threonine metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K10674  ectoine hydroxylase [EC:1.14.11.55]
Other DBs
RHEA: 45743

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