KEGG   ORTHOLOGY: K10814
Entry
K10814                      KO                                     

Name
hcnA
Definition
hydrogen cyanide synthase HcnA [EC:1.4.99.5]
Pathway
ko00460  Cyanoamino acid metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K10814  hcnA; hydrogen cyanide synthase HcnA
 09180 Brite Hierarchies
  09183 Protein families: signaling and cellular processes
   02042 Bacterial toxins
    K10814  hcnA; hydrogen cyanide synthase HcnA
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.99  With unknown physiological acceptors
    1.4.99.5  glycine dehydrogenase (cyanide-forming)
     K10814  hcnA; hydrogen cyanide synthase HcnA
Bacterial toxins [BR:ko02042]
 Not specified
  Hydrogen cyanide production
   K10814  hcnA; hydrogen cyanide synthase HcnA
Other DBs
RN: R00374 R05704
COG: COG0446
GO: 0050622
Genes
VHR: AL538_20020
VCY: IX92_17720
VCT: JV59_21350
SALY: E8E00_15310
SCOT: HBA18_15205
PAE: PA2193(hcnA)
PAEV: N297_2266
PAEI: N296_2266
PAU: PA14_36330(hcnA)
PAP: PSPA7_3103(hcnA)
PAG: PLES_31321(hcnA)
PAF: PAM18_2842(hcnA)
PNC: NCGM2_3175(hcnA)
PAEP: PA1S_14765
PAEM: U769_14365
PAEL: T223_15995
PAEU: BN889_02406(hcnA)
PAEG: AI22_19020
PAEC: M802_2263
PAEO: M801_2265
PFL: PFL_2577(hcnA)
PPRC: PFLCHA0_c26420(hcnA)
PPRO: PPC_2622(hcnA)
PFO: Pfl01_3516(hcnA)
PFE: PSF113_2367(hcnA)
PMAN: OU5_6068
PEN: PSEEN5522(hcnA)
PSEM: TO66_12560
PSEC: CCOS191_5265(hcnA)
PSOS: POS17_2580(hcnA)
PSEN: PNC201_13080(hcnA)
CVI: CV_1684(hcnA)
MES: Meso_3638
SMD: Smed_5072
AGC: BSY240_56
SPHR: BSY17_3519
 » show all
Reference
PMID:233722
  Authors
Castric PA
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
[pae:PA2193]
Reference
PMID:9620970
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence

KEGG   ORTHOLOGY: K10815
Entry
K10815                      KO                                     

Name
hcnB
Definition
hydrogen cyanide synthase HcnB [EC:1.4.99.5]
Pathway
ko00460  Cyanoamino acid metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K10815  hcnB; hydrogen cyanide synthase HcnB
 09180 Brite Hierarchies
  09183 Protein families: signaling and cellular processes
   02042 Bacterial toxins
    K10815  hcnB; hydrogen cyanide synthase HcnB
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.99  With unknown physiological acceptors
    1.4.99.5  glycine dehydrogenase (cyanide-forming)
     K10815  hcnB; hydrogen cyanide synthase HcnB
Bacterial toxins [BR:ko02042]
 Not specified
  Hydrogen cyanide production
   K10815  hcnB; hydrogen cyanide synthase HcnB
Other DBs
RN: R00374 R05704
COG: COG0446
GO: 0050622
Genes
VCY: IX92_17725
VCT: JV59_21345
SALY: E8E00_15315
SCOT: HBA18_15210
PAE: PA2194(hcnB)
PAEV: N297_2267
PAEI: N296_2267
PAU: PA14_36320(hcnB)
PAP: PSPA7_3102(hcnB)
PAG: PLES_31311(hcnB)
PAF: PAM18_2841(hcnB)
PNC: NCGM2_3176(hcnB)
PAEP: PA1S_14760
PAEM: U769_14360
PAEL: T223_15990
PAEU: BN889_02407(hcnB)
PAEG: AI22_19025
PAEC: M802_2264
PAEO: M801_2266
PFL: PFL_2578(hcnB)
PPRC: PFLCHA0_c26430(ooxA)
PPRO: PPC_2623(hcnB)
PFO: Pfl01_3515(hcnB)
PFE: PSF113_2368(hcnB)
PMAN: OU5_6069
PEN: PSEEN5521(hcnB)
PSEM: TO66_12565
PSEC: CCOS191_5264(hcnB)
PSOS: POS17_2581(hcnB)
PSEN: PNC201_13075(hcnB)
CVI: CV_1683(hcnB)
VEI: Veis_0754
 » show all
Reference
PMID:233722
  Authors
Castric PA
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
[pae:PA2194]
Reference
PMID:9620970
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence

KEGG   ORTHOLOGY: K10816
Entry
K10816                      KO                                     

Name
hcnC
Definition
hydrogen cyanide synthase HcnC [EC:1.4.99.5]
Pathway
ko00460  Cyanoamino acid metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K10816  hcnC; hydrogen cyanide synthase HcnC
 09180 Brite Hierarchies
  09183 Protein families: signaling and cellular processes
   02042 Bacterial toxins
    K10816  hcnC; hydrogen cyanide synthase HcnC
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.4  Acting on the CH-NH2 group of donors
   1.4.99  With unknown physiological acceptors
    1.4.99.5  glycine dehydrogenase (cyanide-forming)
     K10816  hcnC; hydrogen cyanide synthase HcnC
Bacterial toxins [BR:ko02042]
 Not specified
  Hydrogen cyanide production
   K10816  hcnC; hydrogen cyanide synthase HcnC
Other DBs
RN: R00374 R05704
COG: COG0665
GO: 0050622
Genes
LVE: 103087047
VEX: VEA_000037
VCY: IX92_17730
VCT: JV59_21340
SALY: E8E00_15320
SCOT: HBA18_15215
PAE: PA2195(hcnC)
PAEV: N297_2268
PAEI: N296_2268
PAU: PA14_36310(hcnC)
PAP: PSPA7_3101(hcnC)
PAG: PLES_31301(hcnC)
PAF: PAM18_2840(hcnC)
PNC: NCGM2_3177(hcnC)
PAEP: PA1S_14755
PAEM: U769_14355
PAEL: T223_15985
PAEU: BN889_02408(hcnC)
PAEG: AI22_19030
PAEC: M802_2265
PAEO: M801_2267
PFL: PFL_2579(hcnC)
PPRO: PPC_2624(hcnC)
PFO: Pfl01_3514(hcnC)
PFE: PSF113_2369(hcnC)
PMAN: OU5_6070
PEN: PSEEN5520(hcnC)
PSEM: TO66_12570
PSEC: CCOS191_5263(hcnC)
PSOS: POS17_2582(hcnC)
PSEN: PNC201_13070(hcnC)
CVI: CV_1682(hcnC)
BPAR: BN117_3795
BPA: BPP0901
BBR: BB0994
AXX: ERS451415_00042(thiO_2)
VEI: Veis_0204
RID: RIdsm_01233(hcnC)
 » show all
Reference
PMID:233722
  Authors
Castric PA
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
[pae:PA2195]
Reference
PMID:9620970
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence

KEGG   ENZYME: 1.4.99.5
Entry
EC 1.4.99.5                 Enzyme                                 

Name
glycine dehydrogenase (cyanide-forming);
hydrogen cyanide synthase;
HCN synthase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With unknown physiological acceptors
Sysname
glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)
Reaction(IUBMB)
glycine + 2 acceptor = hydrogen cyanide + CO2 + 2 reduced acceptor [RN:R05704]
Reaction(KEGG)
R05704 > R00374
Substrate
glycine [CPD:C00037];
acceptor [CPD:C00028]
Product
hydrogen cyanide [CPD:C01326];
CO2 [CPD:C00011];
reduced acceptor [CPD:C00030]
Comment
The enzyme from Pseudomonas sp. contains FAD. The enzyme is membrane-bound, and the 2-electron acceptor is a component of the respiratory chain. The enzyme can act with various artificial electron acceptors, including phenazine methosulfate.
History
EC 1.4.99.5 created 2002
Pathway
ec00460  Cyanoamino acid metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K10814  hydrogen cyanide synthase HcnA
K10815  hydrogen cyanide synthase HcnB
K10816  hydrogen cyanide synthase HcnC
Genes
LVE: 103087047
VEX: VEA_000037
VHR: AL538_20020
VCY: IX92_17720 IX92_17725 IX92_17730
VCT: JV59_21340 JV59_21345 JV59_21350
SALY: E8E00_15310 E8E00_15315 E8E00_15320
SCOT: HBA18_15205 HBA18_15210 HBA18_15215
PAE: PA2193(hcnA) PA2194(hcnB) PA2195(hcnC)
PAEV: N297_2266 N297_2267 N297_2268
PAEI: N296_2266 N296_2267 N296_2268
PAU: PA14_36310(hcnC) PA14_36320(hcnB) PA14_36330(hcnA)
PAP: PSPA7_3101(hcnC) PSPA7_3102(hcnB) PSPA7_3103(hcnA)
PAG: PLES_31301(hcnC) PLES_31311(hcnB) PLES_31321(hcnA)
PAF: PAM18_2840(hcnC) PAM18_2841(hcnB) PAM18_2842(hcnA)
PNC: NCGM2_3175(hcnA) NCGM2_3176(hcnB) NCGM2_3177(hcnC)
PAEU: BN889_02406(hcnA) BN889_02407(hcnB) BN889_02408(hcnC)
PFL: PFL_2577(hcnA) PFL_2578(hcnB) PFL_2579(hcnC)
PPRO: PPC_2622(hcnA) PPC_2623(hcnB) PPC_2624(hcnC)
PFO: Pfl01_3514(hcnC) Pfl01_3515(hcnB) Pfl01_3516(hcnA)
PFE: PSF113_2367(hcnA) PSF113_2368(hcnB) PSF113_2369(hcnC)
PEN: PSEEN5520(hcnC) PSEEN5521(hcnB) PSEEN5522(hcnA)
PSEC: CCOS191_5263(hcnC) CCOS191_5264(hcnB) CCOS191_5265(hcnA)
PSOS: POS17_2580(hcnA) POS17_2581(hcnB) POS17_2582(hcnC)
PSEN: PNC201_13070(hcnC) PNC201_13075(hcnB) PNC201_13080(hcnA)
CVI: CV_1682(hcnC) CV_1683(hcnB) CV_1684(hcnA)
BPAR: BN117_3795
BPA: BPP0901
BBR: BB0994
AXX: ERS451415_00042(thiO_2)
MES: Meso_3638
SMD: Smed_5072
AGC: BSY240_56
RID: RIdsm_01233(hcnC)
SPHR: BSY17_3519
 » show all
Reference
1  [PMID:234422]
  Authors
Wissing F.
  Title
Cyanide production from glycine by a homogenate from a Pseudomonas species.
  Journal
J Bacteriol 121:695-9 (1975)
DOI:10.1128/JB.121.2.695-699.1975
Reference
2  [PMID:233722]
  Authors
Castric PA.
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
Reference
3  [PMID:9620970]
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D.
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence
Reference
4  [PMID:10763748]
  Authors
Blumer C, Haas D.
  Title
Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis.
  Journal
Arch Microbiol 173:170-7 (2000)
DOI:10.1007/s002039900127
Other DBs
ExplorEnz - The Enzyme Database: 1.4.99.5
IUBMB Enzyme Nomenclature: 1.4.99.5
ExPASy - ENZYME nomenclature database: 1.4.99.5
BRENDA, the Enzyme Database: 1.4.99.5

KEGG   REACTION: R05704
Entry
R05704                      Reaction                               

Name
glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)
Definition
Glycine + 2 Acceptor <=> Hydrogen cyanide + CO2 + 2 Reduced acceptor
Equation
Reaction class
RC02808  C00037_C01326
Enzyme
Pathway
rn00460  Cyanoamino acid metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K10814  hydrogen cyanide synthase HcnA [EC:1.4.99.5]
K10815  hydrogen cyanide synthase HcnB [EC:1.4.99.5]
K10816  hydrogen cyanide synthase HcnC [EC:1.4.99.5]
Other DBs
RHEA: 15824

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