KEGG   ORTHOLOGY: K10852Help
Entry
K10852                      KO                                     

Name
PENDE
Definition
isopenicillin-N N-acyltransferase [EC:2.3.1.164]
Pathway
ko00311  Penicillin and cephalosporin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K10852  PENDE; isopenicillin-N N-acyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01002 Peptidases and inhibitors
    K10852  PENDE; isopenicillin-N N-acyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.164  isopenicillin-N N-acyltransferase
     K10852  PENDE; isopenicillin-N N-acyltransferase
Peptidases and inhibitors [BR:ko01002]
 Cysteine peptidases
  Family C45
   K10852  PENDE; isopenicillin-N N-acyltransferase
BRITE hierarchy
Other DBs
RN: R06361
GO: 0050640
Genes
ANI: AN2623.2
AOR: AO090038000545
AFV: AFLA_070880
PCS: Pc21g21370(AAT)
ABE: ARB_02224
TVE: TRV_00551
TaxonomyKoalaUniProt
Reference
PMID:2120195
  Authors
Tobin MB, Fleming MD, Skatrud PL, Miller JR
  Title
Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli.
  Journal
J Bacteriol 172:5908-14 (1990)
DOI:10.1128/JB.172.10.5908-5914.1990
  Sequence
[pcs:Pc21g21370] [ani:AN2623.2]

KEGG   ORTHOLOGY: K19200Help
Entry
K19200                      KO                                     

Name
IAL
Definition
isopenicillin-N N-acyltransferase like protein
Pathway
ko00311  Penicillin and cephalosporin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K19200  IAL; isopenicillin-N N-acyltransferase like protein
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01002 Peptidases and inhibitors
    K19200  IAL; isopenicillin-N N-acyltransferase like protein
Peptidases and inhibitors [BR:ko01002]
 Cysteine peptidases
  Family C45
   K19200  IAL; isopenicillin-N N-acyltransferase like protein
BRITE hierarchy
Genes
FCD: 110855381
QSU: 112011265 112027409
KLA: KLLA0_D15400g
KMX: KLMA_50451
PGU: PGUG_05167
YLI: YALI0E30833g
SLB: AWJ20_170
TTT: THITE_2108911
MTM: MYCTH_2302672
TMN: UCRPA7_2927
MAJ: MAA_07894
MBE: MBM_06424
ANI: AN6775.2
ANG: ANI_1_1226024(An02g08570)
ABE: ARB_03547
TVE: TRV_01006
PEN: PSEEN3262
PKC: PKB_2580
HAM: HALO1436
HBE: BEI_3518
SALN: SALB1_2512
BCJ: BCAM0426
BCEN: DM39_4689
BCEO: I35_4335
BCT: GEM_5292
BCED: DM42_4724
BSEM: WJ12_22165
BPSL: WS57_11465
BMEC: WJ16_21225
AAA: Acav_3440
DAS: Daes_1137
HOH: Hoch_4555
MLO: mll9373
SHUM: STHU_05230
TMO: TMO_b0043
CBEI: LF65_02863
DAE: Dtox_2635
ROP: ROP_43950
SGR: SGR_3008
SBH: SBI_08788
SVE: SVEN_0046
SALU: DC74_7901
SALL: SAZ_40780
SPRI: SPRI_1017
SMAL: SMALA_6283
SFK: KY5_4632
AAU: AAur_1233
BLIN: BLSMQ_3434
NAL: B005_4501
SEN: SACE_5325
SNA: Snas_2859
CWO: Cwoe_4995
CYB: CYB_0863
MRB: Mrub_0051
FLM: MY04_2521
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Garcia-Estrada C, Vaca I, Ullan RV, van den Berg MA, Bovenberg RA, Martin JF
  Title
Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum.
  Journal
BMC Microbiol 9:104 (2009)
DOI:10.1186/1471-2180-9-104
  Sequence
Reference
  Authors
Sprote P, Hynes MJ, Hortschansky P, Shelest E, Scharf DH, Wolke SM, Brakhage AA
  Title
Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster.
  Journal
Mol Microbiol 70:445-61 (2008)
DOI:10.1111/j.1365-2958.2008.06422.x
  Sequence
[ani:AN6775.2]

KEGG   ENZYME: 2.3.1.164Help
Entry
EC 2.3.1.164                Enzyme                                 

Name
isopenicillin-N N-acyltransferase;
acyl-coenzyme A:isopenicillin N acyltransferase;
isopenicillin N:acyl-CoA: acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acyl-CoA:isopenicillin N N-acyltransferase
Reaction(IUBMB)
phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate [RN:R04868]
Reaction(KEGG)
R04868;
(other) R06361
Show
Substrate
phenylacetyl-CoA [CPD:C00582];
isopenicillin N [CPD:C05557];
H2O [CPD:C00001]
Product
CoA [CPD:C00010];
penicillin G [CPD:C05551];
L-2-aminohexanedioate [CPD:C00956]
Comment
Proceeds by a two stage mechanism via 6-aminopenicillanic acid. Different from EC 3.5.1.11, penicillin amidase.
History
EC 2.3.1.164 created 2002
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K10852  isopenicillin-N N-acyltransferase
Genes
ANI: AN2623.2
AOR: AO090038000545
AFV: AFLA_070880
PCS: Pc21g21370(AAT)
ABE: ARB_02224
TVE: TRV_00551
Taxonomy
Reference
1  [PMID:2120195]
  Authors
Tobin MB, Fleming MD, Skatrud PL, Miller JR.
  Title
Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli.
  Journal
J Bacteriol 172:5908-14 (1990)
DOI:10.1128/JB.172.10.5908-5914.1990
  Sequence
[ani:AN2623.2] [pcs:Pc21g21370]
Reference
2  [PMID:8396910]
  Authors
Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ.
  Title
Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry.
  Journal
Biochem J 294 ( Pt 2):357-63 (1993)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.164
IUBMB Enzyme Nomenclature: 2.3.1.164
ExPASy - ENZYME nomenclature database: 2.3.1.164
BRENDA, the Enzyme Database: 2.3.1.164
CAS: 54576-90-8

KEGG   REACTION: R06361Help
Entry
R06361                      Reaction                               

Name
Acyl-CoA:isopenicillin N N-acyltransferase
Definition
Isopenicillin N + Acyl-CoA + H2O <=> Penicillin + CoA + L-2-Aminoadipate
Equation
Comment
specific reaction: R04868
Reaction class
RC00004  C00010_C00040
RC00090  C00956_C05557
RC02908  C00395_C05557
Enzyme
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00672  Penicillin biosynthesis, aminoadipate + cycteine + valine => penicillin
Orthology
K10852  isopenicillin-N N-acyltransferase [EC:2.3.1.164]

DBGET integrated database retrieval system