KEGG   ORTHOLOGY: K10944
Entry
K10944                      KO                                     

Name
pmoA-amoA
Definition
methane/ammonia monooxygenase subunit A [EC:1.14.18.3 1.14.99.39]
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
   00910 Nitrogen metabolism
    K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.3  methane monooxygenase (particulate)
     K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
   1.14.99  Miscellaneous
    1.14.99.39  ammonia monooxygenase
     K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
Other DBs
RN: R00148 R09518
Genes
MCA: MCA1797(pmoA) MCA2854(pmoA2)
METU: GNH96_03570 GNH96_09690
MMT: Metme_0304
MDN: JT25_004545 JT25_012445
MDH: AYM39_18580 AYM39_19770
MKO: MKLM6_3758 MKLM6_3997
METL: U737_00525 U737_22530
MAH: MEALZ_0515(pmoA)
MBUR: EQU24_19305
MPSY: CEK71_17435
NOC: Noc_2502
NHL: Nhal_0676
NWA: Nwat_0632
NTT: TAO_1521(amoA)
NTG: NSCAC_1278(amoA)
HPSE: HPF_13160(pmoA1)
NEU: NE0944(amoA1) NE2063(amoA2)
MSC: BN69_0203(pmoA2) BN69_2827(pmoA) BN69_3534(pmoA)
MIN: Minf_1507(pmoA) Minf_1510(pmoA) Minf_1590(pmoA)
MKC: kam1_1359(pmoA3) kam1_1509(pmoA2) kam1_1512(pmoA1) kam1_1972(pmoA4)
MEAP: MTHMO_0894(pmoA1) MTHMO_0897(pmoA2)
NIO: NITINOP_1853(amoA)
MOX: DAMO_2450(pmoA)
NMR: Nmar_1500
NID: NPIRD3C_2147(amoA)
NIN: NADRNF5_0394(amoA)
NCT: NMSP_0259
NGA: Ngar_c25350(amoA)
NVN: NVIE_027270(amoA)
NEV: NTE_00961
NFN: NFRAN_0675(amoA)
NCV: NCAV_0492(amoA)
NBV: T478_0302
NDV: NDEV_1777(amoA)
AG: CUQ66826(amoA)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
PMID:7590173
  Authors
Holmes AJ, Costello A, Lidstrom ME, Murrell JC
  Title
Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.
  Journal
FEMS Microbiol Lett 132:203-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07834.x
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ORTHOLOGY: K10945
Entry
K10945                      KO                                     

Name
pmoB-amoB
Definition
methane/ammonia monooxygenase subunit B
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10945  pmoB-amoB; methane/ammonia monooxygenase subunit B
   00910 Nitrogen metabolism
    K10945  pmoB-amoB; methane/ammonia monooxygenase subunit B
Other DBs
RN: R00148 R09518
Genes
MCA: MCA1796(pmoB) MCA2853(pmoB2)
METU: GNH96_03575 GNH96_09695
MMT: Metme_0305
MDN: JT25_004540 JT25_012450
MDH: AYM39_18585 AYM39_19765
MKO: MKLM6_3759 MKLM6_3996
METL: U737_00530 U737_22525
MAH: MEALZ_0516(pmoB)
MBUR: EQU24_19300
MPSY: CEK71_17440
NOC: Noc_2501
NHL: Nhal_0677
NWA: Nwat_0633
NTT: TAO_1520(amoB)
NTG: NSCAC_1277(amoB)
HPSE: HPF_13155(pmoB1)
NEU: NE0943(amoB1) NE2062(amoB2)
MSC: BN69_0204(pmoB2) BN69_2828(pmoB) BN69_3535(pmoB)
MIN: Minf_1506(pmoB) Minf_1509(pmoB) Minf_1589(pmoB)
MKC: kam1_1358(pmoB3) kam1_1508(pmoB2) kam1_1511(pmoB1)
MEAP: MTHMO_0895(pmoB1) MTHMO_0898(pmoB2)
NIO: NITINOP_1854(amoB)
MOX: DAMO_2448(pmoB)
NMR: Nmar_1503
NID: NPIRD3C_2150(amoB)
NIN: NADRNF5_0391(amoB)
NCT: NMSP_0256
NGA: Ngar_c25230(amoB)
NVN: NVIE_028430(amoB)
NEV: NTE_01090
NFN: NFRAN_0671(amoB)
NCV: NCAV_0489(amoB)
NBV: T478_0298
NDV: NDEV_1780(amoB)
AG: CUQ66827(amoB)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ORTHOLOGY: K10946
Entry
K10946                      KO                                     

Name
pmoC-amoC
Definition
methane/ammonia monooxygenase subunit C
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10946  pmoC-amoC; methane/ammonia monooxygenase subunit C
   00910 Nitrogen metabolism
    K10946  pmoC-amoC; methane/ammonia monooxygenase subunit C
Other DBs
RN: R00148 R09518
Genes
MCA: MCA0295(pmoC3) MCA1798(pmoC1) MCA2855
METU: GNH96_01890 GNH96_03565 GNH96_09685
MMT: Metme_0303
MDN: JT25_004535 JT25_012440
MDH: AYM39_18590 AYM39_19775
MKO: MKLM6_3760 MKLM6_3998
METL: U737_00535 U737_22535
MAH: MEALZ_0514(pmoC)
MBUR: EQU24_19310
MPSY: CEK71_17430
NOC: Noc_2503
NHL: Nhal_0675
NWA: Nwat_0631
NTT: TAO_1522(amoC)
NTG: NSCAC_1280(amoC)
HPSE: HPF_13165
NEU: NE0945(amoC1) NE1411(amoC3) NE2064(amoC2)
MSC: BN69_0202(pmoC2) BN69_0727 BN69_0852(pmoC) BN69_2826(pmoC) BN69_3533(pmoC)
MIN: Minf_1500(pmoC) Minf_1508(pmoC) Minf_1511(pmoC) Minf_1591(pmoC)
MKC: kam1_1360(pmoC3) kam1_1507(pmoC5) kam1_1510(pmoC2) kam1_1513(pmoC1) kam1_1973(pmoC4)
MEAP: MTHMO_0893(pmoC1) MTHMO_0896(pmoC2)
NIO: NITINOP_0766(amoC3) NITINOP_1259(amoC2) NITINOP_1852(amoC1)
MOX: DAMO_2339(pmoC2) DAMO_2451(pmoC)
NMR: Nmar_1502
NID: NPIRD3C_2149(amoC)
NIN: NADRNF5_0392(amoC)
NCT: NMSP_0257
NGA: Ngar_c25120(amoC1) Ngar_c33570(amoC2)
NVN: NVIE_000540(amoC1) NVIE_002330(amoC2) NVIE_011560(amoC3) NVIE_019080(amoC4) NVIE_024080(amoC5) NVIE_028540(amoC6)
NCV: NCAV_0490(amoC)
NBV: T478_0300
NDV: NDEV_1779(amoC)
AG: CUQ66825(amoC1)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ENZYME: 1.14.18.3
Entry
EC 1.14.18.3                Enzyme                                 

Name
methane monooxygenase (particulate)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
methane,quinol:oxygen oxidoreductase
Reaction(IUBMB)
methane + quinol + O2 = methanol + quinone + H2O [RN:R09518]
Reaction(KEGG)
R09518
Substrate
methane [CPD:C01438];
quinol [CPD:C15603];
O2 [CPD:C00007]
Product
methanol [CPD:C00132];
quinone [CPD:C15602];
H2O [CPD:C00001]
Comment
Contains copper. It is membrane-bound, in contrast to the soluble methane monooxygenase (EC 1.14.13.25).
History
EC 1.14.18.3 created 2011
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
Orthology
K10944  methane/ammonia monooxygenase subunit A
Genes
MCA: MCA1797(pmoA) MCA2854(pmoA2)
METU: GNH96_03570 GNH96_09690
MMT: Metme_0304
MDN: JT25_004545 JT25_012445
MDH: AYM39_18580 AYM39_19770
MKO: MKLM6_3758 MKLM6_3997
METL: U737_00525 U737_22530
MAH: MEALZ_0515(pmoA)
MBUR: EQU24_19305
MPSY: CEK71_17435
NOC: Noc_2502
NHL: Nhal_0676
NWA: Nwat_0632
NTT: TAO_1521(amoA)
NTG: NSCAC_1278(amoA)
HPSE: HPF_13160(pmoA1)
NEU: NE0944(amoA1) NE2063(amoA2)
MSC: BN69_0203(pmoA2) BN69_2827(pmoA) BN69_3534(pmoA)
MIN: Minf_1507(pmoA) Minf_1510(pmoA) Minf_1590(pmoA)
MKC: kam1_1359(pmoA3) kam1_1509(pmoA2) kam1_1512(pmoA1) kam1_1972(pmoA4)
MEAP: MTHMO_0894(pmoA1) MTHMO_0897(pmoA2)
NIO: NITINOP_1853(amoA)
MOX: DAMO_2450(pmoA)
NMR: Nmar_1500
NID: NPIRD3C_2147(amoA)
NIN: NADRNF5_0394(amoA)
NCT: NMSP_0259
NGA: Ngar_c25350(amoA)
NVN: NVIE_027270(amoA)
NEV: NTE_00961
NFN: NFRAN_0675(amoA)
NCV: NCAV_0492(amoA)
NBV: T478_0302
NDV: NDEV_1777(amoA)
 » show all
Reference
1  [PMID:7646068]
  Authors
Shiemke AK, Cook SA, Miley T, Singleton P
  Title
Detergent solubilization of membrane-bound methane monooxygenase requires plastoquinol analogs as electron donors.
  Journal
Arch Biochem Biophys 321:421-8 (1995)
DOI:10.1006/abbi.1995.1413
  Sequence
Reference
2  [PMID:12379148]
  Authors
Basu P, Katterle B, Andersson KK, Dalton H
  Title
The membrane-associated form of methane mono-oxygenase from Methylococcus capsulatus (Bath) is a copper/iron protein.
  Journal
Biochem J 369:417-27 (2003)
DOI:10.1042/BJ20020823
  Sequence
Reference
3  [PMID:16101279]
  Authors
Kitmitto A, Myronova N, Basu P, Dalton H
  Title
Characterization and structural analysis of an active particulate methane monooxygenase trimer from Methylococcus capsulatus (Bath).
  Journal
Biochemistry 44:10954-65 (2005)
DOI:10.1021/bi050820u
  Sequence
Reference
4  [PMID:17444606]
  Authors
Balasubramanian R, Rosenzweig AC
  Title
Structural and mechanistic insights into methane oxidation by particulate methane monooxygenase.
  Journal
Acc Chem Res 40:573-80 (2007)
DOI:10.1021/ar700004s
Other DBs
ExplorEnz - The Enzyme Database: 1.14.18.3
IUBMB Enzyme Nomenclature: 1.14.18.3
ExPASy - ENZYME nomenclature database: 1.14.18.3
BRENDA, the Enzyme Database: 1.14.18.3

KEGG   REACTION: R09518
Entry
R09518                      Reaction                               

Name
methane,quinol:oxygen oxidoreductase
Definition
Methane + Hydroquinone + Oxygen <=> Methanol + Quinone + H2O
Equation
Reaction class
RC00173  C00132_C01438
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Orthology
K10944  methane/ammonia monooxygenase subunit A [EC:1.14.18.3 1.14.99.39]
K10945  methane/ammonia monooxygenase subunit B
K10946  methane/ammonia monooxygenase subunit C
Other DBs
RHEA: 30358

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