KEGG   ORTHOLOGY: K10944
Entry
K10944                      KO                                     

Name
pmoA-amoA
Definition
methane/ammonia monooxygenase subunit A [EC:1.14.18.3 1.14.99.39]
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
   00910 Nitrogen metabolism
    K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.18  With another compound as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.18.3  methane monooxygenase (particulate)
     K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
   1.14.99  Miscellaneous
    1.14.99.39  ammonia monooxygenase
     K10944  pmoA-amoA; methane/ammonia monooxygenase subunit A
Other DBs
RN: R00148 R09518
Genes
MCA: MCA1797(pmoA) MCA2854(pmoA2)
METU: GNH96_03570 GNH96_09690
MMT: Metme_0304
MDN: JT25_004545 JT25_012445
MDH: AYM39_18580 AYM39_19770
MKO: MKLM6_3758 MKLM6_3997
METL: U737_00525 U737_22530
MAH: MEALZ_0515(pmoA)
MBUR: EQU24_19305
MPSY: CEK71_17435
NOC: Noc_2502
NHL: Nhal_0676
NWA: Nwat_0632
NTT: TAO_1521(amoA)
HPSE: HPF_13160(pmoA1)
NEU: NE0944(amoA1) NE2063(amoA2)
MSC: BN69_0203(pmoA2) BN69_2827(pmoA) BN69_3534(pmoA)
MIN: Minf_1507(pmoA) Minf_1510(pmoA) Minf_1590(pmoA)
MKC: kam1_1359(pmoA3) kam1_1509(pmoA2) kam1_1512(pmoA1) kam1_1972(pmoA4)
NIO: NITINOP_1853(amoA)
MOX: DAMO_2450(pmoA)
NMR: Nmar_1500
NID: NPIRD3C_2147(amoA)
NIN: NADRNF5_0394(amoA)
NCT: NMSP_0259
NGA: Ngar_c25350(amoA)
NVN: NVIE_027270(amoA)
NEV: NTE_00961
NFN: NFRAN_0675(amoA)
NCV: NCAV_0492(amoA)
NBV: T478_0302
NDV: NDEV_1777(amoA)
AG: CUQ66826(amoA)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
PMID:7590173
  Authors
Holmes AJ, Costello A, Lidstrom ME, Murrell JC
  Title
Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.
  Journal
FEMS Microbiol Lett 132:203-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07834.x
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ORTHOLOGY: K10945
Entry
K10945                      KO                                     

Name
pmoB-amoB
Definition
methane/ammonia monooxygenase subunit B
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10945  pmoB-amoB; methane/ammonia monooxygenase subunit B
   00910 Nitrogen metabolism
    K10945  pmoB-amoB; methane/ammonia monooxygenase subunit B
Other DBs
RN: R00148 R09518
Genes
MCA: MCA1796(pmoB) MCA2853(pmoB2)
METU: GNH96_03575 GNH96_09695
MMT: Metme_0305
MDN: JT25_004540 JT25_012450
MDH: AYM39_18585 AYM39_19765
MKO: MKLM6_3759 MKLM6_3996
METL: U737_00530 U737_22525
MAH: MEALZ_0516(pmoB)
MBUR: EQU24_19300
MPSY: CEK71_17440
NOC: Noc_2501
NHL: Nhal_0677
NWA: Nwat_0633
NTT: TAO_1520(amoB)
HPSE: HPF_13155(pmoB1)
NEU: NE0943(amoB1) NE2062(amoB2)
MSC: BN69_0204(pmoB2) BN69_2828(pmoB) BN69_3535(pmoB)
MIN: Minf_1506(pmoB) Minf_1509(pmoB) Minf_1589(pmoB)
MKC: kam1_1358(pmoB3) kam1_1508(pmoB2) kam1_1511(pmoB1)
NIO: NITINOP_1854(amoB)
MOX: DAMO_2448(pmoB)
NMR: Nmar_1503
NID: NPIRD3C_2150(amoB)
NIN: NADRNF5_0391(amoB)
NCT: NMSP_0256
NGA: Ngar_c25230(amoB)
NVN: NVIE_028430(amoB)
NEV: NTE_01090
NFN: NFRAN_0671(amoB)
NCV: NCAV_0489(amoB)
NBV: T478_0298
NDV: NDEV_1780(amoB)
AG: CUQ66827(amoB)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ORTHOLOGY: K10946
Entry
K10946                      KO                                     

Name
pmoC-amoC
Definition
methane/ammonia monooxygenase subunit C
Pathway
ko00680  Methane metabolism
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K10946  pmoC-amoC; methane/ammonia monooxygenase subunit C
   00910 Nitrogen metabolism
    K10946  pmoC-amoC; methane/ammonia monooxygenase subunit C
Other DBs
RN: R00148 R09518
Genes
MCA: MCA0295(pmoC3) MCA1798(pmoC1) MCA2855
METU: GNH96_01890 GNH96_03565 GNH96_09685
MMT: Metme_0303
MDN: JT25_004535 JT25_012440
MDH: AYM39_18590 AYM39_19775
MKO: MKLM6_3760 MKLM6_3998
METL: U737_00535 U737_22535
MAH: MEALZ_0514(pmoC)
MBUR: EQU24_19310
MPSY: CEK71_17430
NOC: Noc_2503
NHL: Nhal_0675
NWA: Nwat_0631
NTT: TAO_1522(amoC)
HPSE: HPF_13165
NEU: NE0945(amoC1) NE1411(amoC3) NE2064(amoC2)
MSC: BN69_0202(pmoC2) BN69_0727 BN69_0852(pmoC) BN69_2826(pmoC) BN69_3533(pmoC)
MIN: Minf_1500(pmoC) Minf_1508(pmoC) Minf_1511(pmoC) Minf_1591(pmoC)
MKC: kam1_1360(pmoC3) kam1_1507(pmoC5) kam1_1510(pmoC2) kam1_1513(pmoC1) kam1_1973(pmoC4)
NIO: NITINOP_0766(amoC3) NITINOP_1259(amoC2) NITINOP_1852(amoC1)
MOX: DAMO_2339(pmoC2) DAMO_2451(pmoC)
NMR: Nmar_1502
NID: NPIRD3C_2149(amoC)
NIN: NADRNF5_0392(amoC)
NCT: NMSP_0257
NGA: Ngar_c25120(amoC1) Ngar_c33570(amoC2)
NVN: NVIE_000540(amoC1) NVIE_002330(amoC2) NVIE_011560(amoC3) NVIE_019080(amoC4) NVIE_024080(amoC5) NVIE_028540(amoC6)
NCV: NCAV_0490(amoC)
NBV: T478_0300
NDV: NDEV_1779(amoC)
AG: CUQ66825(amoC1)
 » show all
Reference
  Authors
Stolyar S, Costello AM, Peeples TL, Lidstrom ME
  Title
Role of multiple gene copies in particulate methane monooxygenase activity in the methane-oxidizing bacterium Methylococcus capsulatus Bath.
  Journal
Microbiology 145 ( Pt 5):1235-1244 (1999)
DOI:10.1099/13500872-145-5-1235
  Sequence
Reference
  Authors
Norton JM, Alzerreca JJ, Suwa Y, Klotz MG
  Title
Diversity of ammonia monooxygenase operon in autotrophic ammonia-oxidizing bacteria.
  Journal
Arch Microbiol 177:139-49 (2002)
DOI:10.1007/s00203-001-0369-z

KEGG   ENZYME: 1.14.99.39
Entry
EC 1.14.99.39               Enzyme                                 

Name
ammonia monooxygenase;
AMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
ammonia,donor:oxygen oxidoreductase (hydroxylamine-producing)
Reaction(IUBMB)
NH3 + a reduced acceptor + O2 = NH2OH + an acceptor + H2O [RN:R09519]
Reaction(KEGG)
R09519 > R00148;
(other) R09156
Substrate
NH3 [CPD:C00014];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
NH2OH [CPD:C00192];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
The enzyme catalyses the first reaction in the pathway of ammonia oxidation to nitrite. It contains copper [1], iron [5] and possibly zinc [9]. The enzyme requires two electrons, which are derived indirectly from the quinone pool via a membrane-bound donor.
History
EC 1.14.99.39 created 2010
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10944  methane/ammonia monooxygenase subunit A
Genes
MCA: MCA1797(pmoA) MCA2854(pmoA2)
METU: GNH96_03570 GNH96_09690
MMT: Metme_0304
MDN: JT25_004545 JT25_012445
MDH: AYM39_18580 AYM39_19770
MKO: MKLM6_3758 MKLM6_3997
METL: U737_00525 U737_22530
MAH: MEALZ_0515(pmoA)
MBUR: EQU24_19305
MPSY: CEK71_17435
NOC: Noc_2502
NHL: Nhal_0676
NWA: Nwat_0632
NTT: TAO_1521(amoA)
HPSE: HPF_13160(pmoA1)
NEU: NE0944(amoA1) NE2063(amoA2)
MSC: BN69_0203(pmoA2) BN69_2827(pmoA) BN69_3534(pmoA)
MIN: Minf_1507(pmoA) Minf_1510(pmoA) Minf_1590(pmoA)
MKC: kam1_1359(pmoA3) kam1_1509(pmoA2) kam1_1512(pmoA1) kam1_1972(pmoA4)
NIO: NITINOP_1853(amoA)
MOX: DAMO_2450(pmoA)
NMR: Nmar_1500
NID: NPIRD3C_2147(amoA)
NIN: NADRNF5_0394(amoA)
NCT: NMSP_0259
NGA: Ngar_c25350(amoA)
NVN: NVIE_027270(amoA)
NEV: NTE_00961
NFN: NFRAN_0675(amoA)
NCV: NCAV_0492(amoA)
NBV: T478_0302
NDV: NDEV_1777(amoA)
 » show all
Reference
1  [PMID:8458839]
  Authors
Ensign SA, Hyman MR, Arp DJ
  Title
In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper.
  Journal
J Bacteriol 175:1971-80 (1993)
DOI:10.1128/JB.175.7.1971-1980.1993
Reference
2  [PMID:8085841]
  Authors
Hyman MR, Page CL, Arp DJ
  Title
Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea.
  Journal
Appl Environ Microbiol 60:3033-5 (1994)
DOI:10.1128/AEM.60.8.3033-3035.1994
Reference
3  [PMID:7980540]
  Authors
Bergmann DJ, Hooper AB
  Title
Sequence of the gene, amoB, for the 43-kDa polypeptide of ammonia monoxygenase of Nitrosomonas europaea.
  Journal
Biochem Biophys Res Commun 204:759-62 (1994)
DOI:10.1006/bbrc.1994.2524
  Sequence
Reference
4  [PMID:7590173]
  Authors
Holmes AJ, Costello A, Lidstrom ME, Murrell JC
  Title
Evidence that particulate methane monooxygenase and ammonia monooxygenase may be  evolutionarily related.
  Journal
FEMS Microbiol Lett 132:203-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07834.x
Reference
5  [PMID:8941709]
  Authors
Zahn JA, Arciero DM, Hooper AB, DiSpirito AA
  Title
Evidence for an iron center in the ammonia monooxygenase from Nitrosomonas europaea.
  Journal
FEBS Lett 397:35-8 (1996)
DOI:10.1016/S0014-5793(96)01116-7
Reference
6  [PMID:8654570]
  Authors
Moir JW, Crossman LC, Spiro S, Richardson DJ
  Title
The purification of ammonia monooxygenase from Paracoccus denitrificans.
  Journal
FEBS Lett 387:71-4 (1996)
DOI:10.1016/0014-5793(96)00463-2
Reference
7  [PMID:11004450]
  Authors
Whittaker M, Bergmann D, Arciero D, Hooper AB.
  Title
Electron transfer during the oxidation of ammonia by the chemolithotrophic bacterium Nitrosomonas europaea.
  Journal
Biochim Biophys Acta 1459:346-55 (2000)
DOI:10.1016/S0005-2728(00)00171-7
Reference
8  [PMID:12209257]
  Authors
Arp DJ, Sayavedra-Soto LA, Hommes NG.
  Title
Molecular biology and biochemistry of ammonia oxidation by Nitrosomonas europaea.
  Journal
Arch Microbiol 178:250-5 (2002)
DOI:10.1007/s00203-002-0452-0
Reference
9  [PMID:19453274]
  Authors
Gilch S, Meyer O, Schmidt I
  Title
A soluble form of ammonia monooxygenase in Nitrosomonas europaea.
  Journal
Biol Chem 390:863-73 (2009)
DOI:10.1515/BC.2009.085
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.39
IUBMB Enzyme Nomenclature: 1.14.99.39
ExPASy - ENZYME nomenclature database: 1.14.99.39
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.99.39
BRENDA, the Enzyme Database: 1.14.99.39

KEGG   REACTION: R00148
Entry
R00148                      Reaction                               

Name
ammonia,ubiquinol:oxygen oxidoreductase;
ammonia monooxygenase
Definition
Ammonia + Oxygen + Ubiquinol <=> Hydroxylamine + H2O + Ubiquinone
Equation
Reaction class
RC00061  C00390_C00399
RC02797  C00014_C00192
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00528  Nitrification, ammonia => nitrite
M00804  Complete nitrification, comammox, ammonia => nitrite => nitrate
Orthology
K10944  methane/ammonia monooxygenase subunit A [EC:1.14.18.3 1.14.99.39]
K10945  methane/ammonia monooxygenase subunit B
K10946  methane/ammonia monooxygenase subunit C

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