KEGG   ORTHOLOGY: K12635Help
Entry
K12635                      KO                                     

Name
dmpM
Definition
O-demethylpuromycin O-methyltransferase [EC:2.1.1.38]
Pathway
ko00998  Biosynthesis of various secondary metabolites - part 2
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various secondary metabolites - part 2
    K12635  dmpM; O-demethylpuromycin O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.38  O-demethylpuromycin O-methyltransferase
     K12635  dmpM; O-demethylpuromycin O-methyltransferase
BRITE hierarchy
Other DBs
RN: R05676
GO: 0030739
Genes
AG: AAB00531(dmpM)
TaxonomyKoalaUniProt
Reference
PMID:1756982
  Authors
Lacalle RA, Ruiz D, Jimenez A
  Title
Molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase from Streptomyces alboniger.
  Journal
Gene 109:55-61 (1991)
DOI:10.1016/0378-1119(91)90588-3
  Sequence

KEGG   ENZYME: 2.1.1.38Help
Entry
EC 2.1.1.38                 Enzyme                                 

Name
O-demethylpuromycin O-methyltransferase;
O-demethylpuromycin methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:O-demethylpuromycin O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + O-demethylpuromycin = S-adenosyl-L-homocysteine + puromycin [RN:R03633]
Reaction(KEGG)
R03633;
(other) R05676
Show
Substrate
S-adenosyl-L-methionine [CPD:C00019];
O-demethylpuromycin [CPD:C02864]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
puromycin [CPD:C01610]
Comment
Puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.
History
EC 2.1.1.38 created 1972
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K12635  O-demethylpuromycin O-methyltransferase
Reference
1  [PMID:5773275]
  Authors
Rao MM, Rebello PF, Pogell BM.
  Title
Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin.
  Journal
J Biol Chem 244:112-8 (1969)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.38
IUBMB Enzyme Nomenclature: 2.1.1.38
ExPASy - ENZYME nomenclature database: 2.1.1.38
BRENDA, the Enzyme Database: 2.1.1.38
CAS: 37257-04-8

KEGG   REACTION: R05676Help
Entry
R05676                      Reaction                               

Definition
N-Acetyl-O-demethylpuromycin + S-Adenosyl-L-methionine <=> N-Acetylpuromycin + S-Adenosyl-L-homocysteine
Equation
Comment
DmpM
Ref.1. The Journal of Biological chemistry; vol.271, No.3, Jan 19,
pp.1579-1590, 1996
Reaction class
RC00003  C00019_C00021
RC00392  C07031_C07032
Enzyme
Pathway
rn00998  Biosynthesis of various secondary metabolites - part 2
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K12635  O-demethylpuromycin O-methyltransferase [EC:2.1.1.38]

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