KEGG   ORTHOLOGY: K12676Help
Entry
K12676                      KO                                     

Name
pah
Definition
proclavaminate amidinohydrolase [EC:3.5.3.22]
Pathway
ko00331  Clavulanic acid biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00674  Clavaminate biosynthesis, arginine + glyceraldehyde-3P => clavaminate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00331 Clavulanic acid biosynthesis
    K12676  pah; proclavaminate amidinohydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.3  In linear amidines
    3.5.3.22  proclavaminate amidinohydrolase
     K12676  pah; proclavaminate amidinohydrolase
BRITE hierarchy
Other DBs
RN: R05357
GO: 0033972
Genes
SFA: Sfla_0557
STRP: F750_6322
SCLF: BB341_07815
SFK: KY5_8016c
KAB: B7C62_18895
KAU: B6264_26745
SVI: Svir_33400
TaxonomyKoalaUniProt
Reference
PMID:7601835
  Authors
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
  Title
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
  Journal
J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995

KEGG   ENZYME: 3.5.3.22Help
Entry
EC 3.5.3.22                 Enzyme                                 

Name
proclavaminate amidinohydrolase;
PAH;
proclavaminate amidino hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines
BRITE hierarchy
Sysname
amidinoproclavaminate amidinohydrolase
Reaction(IUBMB)
amidinoproclavaminate + H2O = proclavaminate + urea [RN:R05357]
Reaction(KEGG)
Substrate
amidinoproclavaminate [CPD:C06657];
H2O [CPD:C00001]
Product
proclavaminate [CPD:C06658];
urea [CPD:C00086]
Comment
Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. It carries out an intermediary reaction between the first reaction of EC 1.14.11.21, clavaminate synthase, and the second and third reactions of that enzyme. Requires Mn2+.
History
EC 3.5.3.22 created 2003
Pathway
ec00331  Clavulanic acid biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K12676  proclavaminate amidinohydrolase
Genes
SFA: Sfla_0557
STRP: F750_6322
SCLF: BB341_07815
SFK: KY5_8016c
KAB: B7C62_18895
KAU: B6264_26745
SVI: Svir_33400
Taxonomy
Reference
1  [PMID:1998687]
  Authors
Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA.
  Title
Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction.
  Journal
Biochemistry 30:2281-92 (1991)
Reference
2  [PMID:11472170]
  Authors
Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI.
  Title
Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities.
  Journal
J Am Chem Soc 123:7388-98 (2001)
DOI:10.1021/ja004025+
Reference
3  [PMID:12413541]
  Authors
Townsend CA.
  Title
New reactions in clavulanic acid biosynthesis.
  Journal
Curr Opin Chem Biol 6:583-9 (2002)
DOI:10.1016/S1367-5931(02)00392-7
Reference
4  [PMID:7601835]
  Authors
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA.
  Title
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
  Journal
J Bacteriol 177:3714-20 (1995)
DOI:10.1128/JB.177.13.3714-3720.1995
Other DBs
ExplorEnz - The Enzyme Database: 3.5.3.22
IUBMB Enzyme Nomenclature: 3.5.3.22
ExPASy - ENZYME nomenclature database: 3.5.3.22
BRENDA, the Enzyme Database: 3.5.3.22

KEGG   REACTION: R05357Help
Entry
R05357                      Reaction                               

Name
amidinoproclavaminate amidinohydrolase
Definition
Guanidinoproclavaminic acid + H2O <=> Proclavaminic acid + Urea
Equation
Comment
pah gene
Reaction class
RC00024  C06657_C06658
RC00329  C00086_C06657
Enzyme
Pathway
rn00331  Clavulanic acid biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00674  Clavaminate biosynthesis, arginine + glyceraldehyde-3P => clavaminate
Orthology
K12676  proclavaminate amidinohydrolase [EC:3.5.3.22]
Reference
1  [PMID:9614345]
  Authors
McGowan SJ, Bycroft BW, Salmond GP.
  Title
Bacterial production of carbapenems and clavams: evolution of beta-lactam antibiotic pathways.
  Journal
Trends Microbiol 6:203-8 (1998)
DOI:10.1016/S0966-842X(98)01251-7
Other DBs
RHEA: 17004

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