KEGG   ORTHOLOGY: K12692
Entry
K12692            Tight     KO                                     

Name
H6H
Definition
hyoscyamine (6S)-dioxygenase [EC:1.14.11.11]
Pathway
ko00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00960 Tropane, piperidine and pyridine alkaloid biosynthesis
    K12692  H6H; hyoscyamine (6S)-dioxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.11  hyoscyamine (6S)-dioxygenase
     K12692  H6H; hyoscyamine (6S)-dioxygenase
Other DBs
RN: R03812
GO: 0047998
Genes
AG: BAA05630(H6H)
Reference
PMID:8066129
  Authors
Kanegae T, Kajiya H, Amano Y, Hashimoto T, Yamada Y
  Title
Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle.
  Journal
Plant Physiol 105:483-90 (1994)
DOI:10.1104/pp.105.2.483
  Sequence

KEGG   ENZYME: 1.14.11.11
Entry
EC 1.14.11.11               Enzyme                                 

Name
hyoscyamine (6S)-dioxygenase;
hyoscyamine 6beta-hydroxylase;
hyoscyamine 6beta-dioxygenase;
hyoscyamine 6-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
L-hyoscyamine,2-oxoglutarate:oxygen oxidoreductase [(6S)-hydroxylating]
Reaction(IUBMB)
L-hyoscyamine + 2-oxoglutarate + O2 = (6S)-hydroxyhyoscyamine + succinate + CO2 [RN:R03812]
Reaction(KEGG)
R03812
Substrate
L-hyoscyamine [CPD:C02046];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(6S)-hydroxyhyoscyamine [CPD:C03325];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate.
History
EC 1.14.11.11 created 1989
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12692  hyoscyamine (6S)-dioxygenase
Reference
1  [PMID:16664866]
  Authors
Hashimoto T, Yamada Y.
  Title
Hyoscyamine 6beta-Hydroxylase, a 2-Oxoglutarate-Dependent Dioxygenase, in Alkaloid-Producing Root Cultures.
  Journal
Plant Physiol 81:619-625 (1986)
DOI:10.1104/pp.81.2.619
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.11
IUBMB Enzyme Nomenclature: 1.14.11.11
ExPASy - ENZYME nomenclature database: 1.14.11.11
BRENDA, the Enzyme Database: 1.14.11.11
CAS: 103865-33-4

KEGG   REACTION: R03812
Entry
R03812                      Reaction                               

Name
L-Hyoscyamine,2-oxoglytarate:oxygen oxidoreductase ((6S)-hydroxylating)
Definition
L-Hyoscyamine + 2-Oxoglutarate + Oxygen <=> (6S)-Hydroxyhyoscyamine + Succinate + CO2
Equation
Reaction class
RC00661  C02046_C03325
Enzyme
Pathway
rn00960  Tropane, piperidine and pyridine alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K12692  hyoscyamine (6S)-dioxygenase [EC:1.14.11.11]
Other DBs
RHEA: 12632

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