KEGG   ORTHOLOGY: K12712
Entry
K12712                      KO                                     

Name
novP, cloP, couP
Definition
demethyldecarbamoylnovobiocin O-methyltransferase [EC:2.1.1.285]
Pathway
ko00401  Novobiocin biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12712  novP, cloP, couP; demethyldecarbamoylnovobiocin O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.285  demethyldecarbamoylnovobiocin O-methyltransferase
     K12712  novP, cloP, couP; demethyldecarbamoylnovobiocin O-methyltransferase
Other DBs
RN: R06767 R06771 R06774
Genes
NGV: CDO52_02225
AG: AAF67509(novP) AAG29794(couP) AAN65238(cloP)
Reference
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
Reference
  Authors
Pojer F, Li SM, Heide L.
  Title
Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster: new insights into the biosynthesis of aminocoumarin antibiotics.
  Journal
Microbiology 148:3901-11 (2002)
DOI:10.1099/00221287-148-12-3901
  Sequence
Reference
  Authors
Freel Meyers CL, Oberthur M, Heide L, Kahne D, Walsh CT
  Title
Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.
  Journal
Biochemistry 43:15022-36 (2004)
DOI:10.1021/bi048457z

KEGG   ENZYME: 2.1.1.285
Entry
EC 2.1.1.285                Enzyme                                 

Name
demethyldecarbamoylnovobiocin O-methyltransferase;
NovP
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:demethyldecarbamoylnovobiocin 4''-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + demethyldecarbamoylnovobiocin = S-adenosyl-L-homocysteine + decarbamoylnovobiocin [RN:R06771]
Reaction(KEGG)
R06771
Substrate
S-adenosyl-L-methionine [CPD:C00019];
demethyldecarbamoylnovobiocin [CPD:C12475]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
decarbamoylnovobiocin [CPD:C12476]
Comment
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.
History
EC 2.1.1.285 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12712  demethyldecarbamoylnovobiocin O-methyltransferase
Genes
NGV: CDO52_02225
Reference
1  [PMID:14694473]
  Authors
Freel Meyers CL, Oberthur M, Xu H, Heide L, Kahne D, Walsh CT
  Title
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring.
  Journal
Angew Chem Int Ed Engl 43:67-70 (2004)
DOI:10.1002/anie.200352626
  Sequence
Reference
2  [PMID:19857499]
  Authors
Gomez Garcia I, Stevenson CE, Uson I, Freel Meyers CL, Walsh CT, Lawson DM
  Title
The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily.
  Journal
J Mol Biol 395:390-407 (2010)
DOI:10.1016/j.jmb.2009.10.045
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.285
IUBMB Enzyme Nomenclature: 2.1.1.285
ExPASy - ENZYME nomenclature database: 2.1.1.285
BRENDA, the Enzyme Database: 2.1.1.285

KEGG   REACTION: R06771
Entry
R06771                      Reaction                               

Name
S-adenosyl-L-methionine:demethyldecarbamoylnovobiocin 4''-O-methyltransferase
Definition
Demethyldecarbamoylnovobiocin + S-Adenosyl-L-methionine <=> Decarbamoylnovobiocin + S-Adenosyl-L-homocysteine
Equation
Reaction class
RC00003  C00019_C00021
RC00466  C12475_C12476
Enzyme
Pathway
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K12712  demethyldecarbamoylnovobiocin O-methyltransferase [EC:2.1.1.285]
Other DBs
RHEA: 36658

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