KEGG   ORTHOLOGY: K12747Help
Entry
K12747                      KO                                     

Name
cefG
Definition
deacetylcephalosporin-C acetyltransferase [EC:2.3.1.175]
Pathway
ko00311  Penicillin and cephalosporin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K12747  cefG; deacetylcephalosporin-C acetyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.175  deacetylcephalosporin-C acetyltransferase
     K12747  cefG; deacetylcephalosporin-C acetyltransferase
BRITE hierarchy
Other DBs
RN: R03064
GO: 0033813
Genes
AG: AAA32673(cefG)
TaxonomyKoalaUniProt
Reference
PMID:1569032
  Authors
Gutierrez S, Velasco J, Fernandez FJ, Martin JF
  Title
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.
  Journal
J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992
  Sequence

KEGG   ORTHOLOGY: K01060Help
Entry
K01060                      KO                                     

Name
cah
Definition
cephalosporin-C deacetylase [EC:3.1.1.41]
Pathway
ko00311  Penicillin and cephalosporin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00311 Penicillin and cephalosporin biosynthesis
    K01060  cah; cephalosporin-C deacetylase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.1  Carboxylic-ester hydrolases
    3.1.1.41  cephalosporin-C deacetylase
     K01060  cah; cephalosporin-C deacetylase
BRITE hierarchy
Other DBs
RN: R03062
COG: COG3458
GO: 0047739
Genes
CATE: C2869_07180
MMT: Metme_1885
MDN: JT25_021245
MDH: AYM39_10145
MKO: MKLM6_2137
METL: U737_14500
MPSY: CEK71_05805
MMAI: sS8_2978 sS8_3683
TVI: Thivi_2764
TEE: Tel_08835
MLO: mlr6993
ARA: Arad_9051
RLE: RL2908
RLG: Rleg_2440
MGM: Mmc1_2518
BSU: BSU03180(cah)
BSR: I33_0363
BSL: A7A1_1880
BSH: BSU6051_03180(cah)
BSUT: BSUB_00367(cah)
BSUL: BSUA_00367(cah)
BSUS: Q433_01915
BSS: BSUW23_01625(cah)
BST: GYO_0523
BSO: BSNT_06647(cah)
BSQ: B657_03180(cah)
BSX: C663_0308(cah)
BLI: BL01713(cah)
BLD: BLi00379(cah)
BLH: BaLi_c03950(cah)
BAQ: BACAU_0285(cah)
BYA: BANAU_0284(cah)
BAMP: B938_01465
BAML: BAM5036_0301(cah)
BAMA: RBAU_0315(cah)
BAMN: BASU_0300(cah)
BAMB: BAPNAU_0285(cah)
BAMT: AJ82_01875
BAMY: V529_03050(cah)
BMP: NG74_00324(cah)
BAO: BAMF_0291(cah)
BAZ: BAMTA208_01460(cah)
BQL: LL3_00299(cah)
BXH: BAXH7_00302(cah)
BQY: MUS_0300(cah)
BAMI: KSO_017950
BAMC: U471_03020
BAMF: U722_01760
BHA: BH3326
BCL: ABC1769(cah)
BPU: BPUM_0297
BPUM: BW16_01880
BPUS: UP12_01885
BJS: MY9_0327
BACW: QR42_01840
BACP: SB24_08095
BACB: OY17_04375
BACY: QF06_00545
BACL: BS34A_03730(cah)
BALM: BsLM_0331
BGY: BGLY_0386(cah)
BBEV: BBEV_0471(cah)
AXL: AXY_21800(axe) AXY_22340(axe)
BSE: Bsel_0980
PPM: PPSC2_18660(cah3) PPSC2_24180(cah5)
PPO: PPM_3754(cah3) PPM_4801(cah5)
PSWU: SY83_03310
COHN: KCTCHS21_15190(cah) KCTCHS21_52180(axe1)
LLK: LLKF_1447(axe)
LLX: NCDO2118_1409(axe)
LLR: llh_7440
LLI: uc509_1372(axe)
LLW: kw2_1343
LLJ: LG36_1029(axe)
SPN: SP_1695
SPD: SPD_1506
SPR: spr1538(axe1)
SPW: SPCG_1668(axe1)
SJJ: SPJ_1589
SPX: SPG_1602(axe1)
SPV: SPH_1800
SPP: SPP_1712
SNP: SPAP_1703
SAG: SAG0041
SAN: gbs0040
SAK: SAK_0074
SGC: A964_0040
SAGM: BSA_740
SAGR: SAIL_770
SAGE: EN72_00375
SAGG: EN73_00375
SAGN: W903_0076(axe1)
SSA: SSA_0070(axe1)
SEZ: Sez_0253
SEQ: SZO_17040
SEQU: Q426_07955
SUB: SUB1651
SGT: SGGB_0215(axe1)
SMB: smi_0600
SOR: SOR_0547
STB: SGPB_0158(axe1)
SCF: Spaf_1050
SMN: SMA_192(cah)
STRN: SNAG_0541(axeA)
EFA: EF1236
EFL: EF62_1680
EFS: EFS1_1059
EFQ: DR75_300
ENE: ENT_06680
CPE: CPE1596
CPF: CPF_1848(axe1)
CBV: U729_2266(axe1)
CTH: Cthe_3063
HSC: HVS_10845(cah)
CCE: Ccel_0210
CSS: Cst_c14440(cah)
CSD: Clst_1394
CCEL: CCDG5_2025
BPRO: PMF13cell1_02357(cah)
DSY: DSY0351
DHD: Dhaf_0284
SGY: Sgly_0270
TIT: Thit_0191
CSC: Csac_2436
ATE: Athe_0152
TTM: Tthe_0984
SCO: SCO7057(SC4G1.23c)
SALB: XNR_3892
SMA: SAVERM_1302(axe1) SAVERM_1457(axe2)
SGB: WQO_23395
SHY: SHJG_2291
SVE: SVEN_6682
SALS: SLNWT_7151
SFI: SFUL_4907
SALU: DC74_6913
SALL: SAZ_35900
SLV: SLIV_03965(axeA)
SGU: SGLAU_03235(axeA)
STRE: GZL_01842
SLD: T261_1053
SAMB: SAM23877_1034(axeA)
SRW: TUE45_01052(axeA)
SLE: sle_63340(sle_63340)
SRN: A4G23_05285(axeA)
SNR: SNOUR_08235(axeA)
SMAL: SMALA_1210
SGE: DWG14_02113(axeA_1) DWG14_07762(axeA_2)
KSK: KSE_04670(axe1) KSE_10120(axe2)
CMS: CMS0795
CMC: CMN_00165
MOO: BWL13_02328(axeA)
ART: Arth_3303
ARR: ARUE_c34390(cah)
ARM: ART_3104
AAU: AAur_3303
ACH: Achl_3101
JDE: Jden_1761
XCE: Xcel_2723
IDO: I598_3177(axeA)
CFL: Cfla_0926
PACC: PAC1_01355
PACH: PAGK_0284
CACN: RN83_01750
ACIJ: JS278_00650(axeA)
KFL: Kfla_0274
STRR: EKD16_07995(axeA)
SRO: Sros_2720
AMI: Amir_1984
SESP: BN6_23910
AHG: AHOG_17940(axeA)
ALO: CRK56630
SAQ: Sare_3004
ASE: ACPL_5484(axe1)
ACTN: L083_3285(axe1)
ACTS: ACWT_5353
SNA: Snas_3115
TBI: Tbis_1965
APV: Apar_0931
OLS: Olsu_1284
RCA: Rcas_1231
HAU: Haur_0886
ATM: ANT_24920
PBF: CFX0092_B0571(axeA)
TTR: Tter_0201
PBAS: SMSP2_02919(axeA)
LIE: LIF_A1498
LIS: LIL_12147
LBF: LBF_1671
LST: LSS_18918
BRM: Bmur_1237
BPW: WESB_0039
BIP: Bint_2614
BXY: BXY_25990
FLN: FLA_1626
MUC: MuYL_4073
TMW: THMA_0073
TMQ: THMB_0073
TMX: THMC_0073
TPT: Tpet_0847
TRQ: TRQ2_0870
TNA: CTN_0615
TNP: Tnap_0707
TLE: Tlet_0356
DTU: Dtur_0265
 » show all
TaxonomyKoalaUniProt

KEGG   ENZYME: 2.3.1.175Help
Entry
EC 2.3.1.175                Enzyme                                 

Name
deacetylcephalosporin-C acetyltransferase;
acetyl-CoA:deacetylcephalosporin-C acetyltransferase;
DAC acetyltransferase;
cefG;
deacetylcephalosporin C acetyltransferase;
acetyl coenzyme A:DAC acetyltransferase;
acetyl-CoA:DAC acetyltransferase;
CPC acetylhydrolase;
acetyl-CoA:DAC O-acetyltransferase;
DAC-AT
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C [RN:R03064]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
deacetylcephalosporin C [CPD:C03112]
Product
CoA [CPD:C00010];
cephalosporin C [CPD:C00916]
Comment
This enzyme catalyses the final step in the biosynthesis of cephalosporin C.
History
EC 2.3.1.175 created 2005
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K12747  deacetylcephalosporin-C acetyltransferase
Reference
1  [PMID:1368946]
  Authors
Matsuyama K, Matsumoto H, Matsuda A, Sugiura H, Komatsu K, Ichikawa S.
  Title
Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.
  Journal
Biosci Biotechnol Biochem 56:1410-2 (1992)
DOI:10.1271/bbb.56.1410
Reference
2  [PMID:1569032]
  Authors
Gutierrez S, Velasco J, Fernandez FJ, Martin JF.
  Title
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.
  Journal
J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992
  Sequence
Reference
3  [PMID:1632779]
  Authors
Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K.
  Title
Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum.
  Journal
Biochem Biophys Res Commun 186:40-6 (1992)
DOI:10.1016/S0006-291X(05)80772-7
  Sequence
Reference
4  [PMID:9421924]
  Authors
Gutierrez S, Velasco J, Marcos AT, Fernandez FJ, Fierro F, Barredo JL, Diez B, Martin JF.
  Title
Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum.
  Journal
Appl Microbiol Biotechnol 48:606-14 (1997)
Reference
5  [PMID:9895280]
  Authors
Velasco J, Gutierrez S, Campoy S, Martin JF.
  Title
Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits.
  Journal
Biochem J 337 ( Pt 3):379-85 (1999)
Reference
6  [PMID:7847890]
  Authors
Martin JF, Gutierrez S, Fernandez FJ, Velasco J, Fierro F, Marcos AT, Kosalkova K.
  Title
Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.
  Journal
Antonie Van Leeuwenhoek 65:227-43 (1994)
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.175
IUBMB Enzyme Nomenclature: 2.3.1.175
ExPASy - ENZYME nomenclature database: 2.3.1.175
BRENDA, the Enzyme Database: 2.3.1.175
CAS: 57827-76-6

KEGG   ENZYME: 3.1.1.41Help
Entry
EC 3.1.1.41                 Enzyme                                 

Name
cephalosporin-C deacetylase;
cephalosporin C acetyl-hydrolase;
cephalosporin C acetylase;
cephalosporin acetylesterase;
cephalosporin C acetylesterase;
cephalosporin C acetyl-esterase;
cephalosporin C deacetylase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
cephalosporin-C acetylhydrolase
Reaction(IUBMB)
cephalosporin C + H2O = deacetylcephalosporin C + acetate [RN:R03062]
Reaction(KEGG)
Substrate
cephalosporin C [CPD:C00916];
H2O [CPD:C00001]
Product
deacetylcephalosporin C [CPD:C03112];
acetate [CPD:C00033]
Comment
Hydrolyses the acetyl ester bond on the 10-position of the antibiotic cephalosporin C.
History
EC 3.1.1.41 created 1976
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K01060  cephalosporin-C deacetylase
Genes
CATE: C2869_07180
MMT: Metme_1885
MDN: JT25_021245
MDH: AYM39_10145
MKO: MKLM6_2137
METL: U737_14500
MPSY: CEK71_05805
MMAI: sS8_2978 sS8_3683
TVI: Thivi_2764
TEE: Tel_08835
MLO: mlr6993
ARA: Arad_9051
RLE: RL2908
RLG: Rleg_2440
MGM: Mmc1_2518
BSU: BSU03180(cah)
BSR: I33_0363
BSL: A7A1_1880
BSH: BSU6051_03180(cah)
BSUT: BSUB_00367(cah)
BSUL: BSUA_00367(cah)
BSUS: Q433_01915
BSS: BSUW23_01625(cah)
BST: GYO_0523
BSO: BSNT_06647(cah)
BSQ: B657_03180(cah)
BSX: C663_0308(cah)
BLI: BL01713(cah)
BLD: BLi00379(cah)
BLH: BaLi_c03950(cah)
BAQ: BACAU_0285(cah)
BYA: BANAU_0284(cah)
BAMP: B938_01465
BAML: BAM5036_0301(cah)
BAMA: RBAU_0315(cah)
BAMN: BASU_0300(cah)
BAMB: BAPNAU_0285(cah)
BAMT: AJ82_01875
BAMY: V529_03050(cah)
BMP: NG74_00324(cah)
BAO: BAMF_0291(cah)
BAZ: BAMTA208_01460(cah)
BQL: LL3_00299(cah)
BXH: BAXH7_00302(cah)
BQY: MUS_0300(cah)
BAMI: KSO_017950
BAMC: U471_03020
BAMF: U722_01760
BHA: BH3326
BCL: ABC1769(cah)
BPU: BPUM_0297
BPUM: BW16_01880
BPUS: UP12_01885
BJS: MY9_0327
BACW: QR42_01840
BACP: SB24_08095
BACB: OY17_04375
BACY: QF06_00545
BACL: BS34A_03730(cah)
BALM: BsLM_0331
BGY: BGLY_0386(cah)
BBEV: BBEV_0471(cah)
AXL: AXY_21800(axe) AXY_22340(axe)
BSE: Bsel_0980
PPM: PPSC2_18660(cah3) PPSC2_24180(cah5)
PPO: PPM_3754(cah3) PPM_4801(cah5)
PSWU: SY83_03310
COHN: KCTCHS21_15190(cah) KCTCHS21_52180(axe1)
LLK: LLKF_1447(axe)
LLX: NCDO2118_1409(axe)
LLR: llh_7440
LLI: uc509_1372(axe)
LLW: kw2_1343
LLJ: LG36_1029(axe)
SPN: SP_1695
SPD: SPD_1506
SPR: spr1538(axe1)
SPW: SPCG_1668(axe1)
SJJ: SPJ_1589
SPX: SPG_1602(axe1)
SPV: SPH_1800
SPP: SPP_1712
SNP: SPAP_1703
SAG: SAG0041
SAN: gbs0040
SAK: SAK_0074
SGC: A964_0040
SAGM: BSA_740
SAGR: SAIL_770
SAGE: EN72_00375
SAGG: EN73_00375
SAGN: W903_0076(axe1)
SSA: SSA_0070(axe1)
SEZ: Sez_0253
SEQ: SZO_17040
SEQU: Q426_07955
SUB: SUB1651
SGT: SGGB_0215(axe1)
SMB: smi_0600
SOR: SOR_0547
STB: SGPB_0158(axe1)
SCF: Spaf_1050
SMN: SMA_192(cah)
STRN: SNAG_0541(axeA)
EFA: EF1236
EFL: EF62_1680
EFS: EFS1_1059
EFQ: DR75_300
ENE: ENT_06680
CPE: CPE1596
CPF: CPF_1848(axe1)
CBV: U729_2266(axe1)
CTH: Cthe_3063
HSC: HVS_10845(cah)
CCE: Ccel_0210
CSS: Cst_c14440(cah)
CSD: Clst_1394
CCEL: CCDG5_2025
BPRO: PMF13cell1_02357(cah)
DSY: DSY0351
DHD: Dhaf_0284
SGY: Sgly_0270
TIT: Thit_0191
CSC: Csac_2436
ATE: Athe_0152
TTM: Tthe_0984
SCO: SCO7057(SC4G1.23c)
SALB: XNR_3892
SMA: SAVERM_1302(axe1) SAVERM_1457(axe2)
SGB: WQO_23395
SHY: SHJG_2291
SVE: SVEN_6682
SALS: SLNWT_7151
SFI: SFUL_4907
SALU: DC74_6913
SALL: SAZ_35900
SLV: SLIV_03965(axeA)
SGU: SGLAU_03235(axeA)
STRE: GZL_01842
SLD: T261_1053
SAMB: SAM23877_1034(axeA)
SRW: TUE45_01052(axeA)
SLE: sle_63340(sle_63340)
SRN: A4G23_05285(axeA)
SNR: SNOUR_08235(axeA)
SMAL: SMALA_1210
SGE: DWG14_02113(axeA_1) DWG14_07762(axeA_2)
KSK: KSE_04670(axe1) KSE_10120(axe2)
CMS: CMS0795
CMC: CMN_00165
MOO: BWL13_02328(axeA)
ART: Arth_3303
ARR: ARUE_c34390(cah)
ARM: ART_3104
AAU: AAur_3303
ACH: Achl_3101
JDE: Jden_1761
XCE: Xcel_2723
IDO: I598_3177(axeA)
CFL: Cfla_0926
PACC: PAC1_01355
PACH: PAGK_0284
CACN: RN83_01750
ACIJ: JS278_00650(axeA)
KFL: Kfla_0274
STRR: EKD16_07995(axeA)
SRO: Sros_2720
AMI: Amir_1984
SESP: BN6_23910
AHG: AHOG_17940(axeA)
ALO: CRK56630
SAQ: Sare_3004
ASE: ACPL_5484(axe1)
ACTN: L083_3285(axe1)
ACTS: ACWT_5353
SNA: Snas_3115
TBI: Tbis_1965
APV: Apar_0931
OLS: Olsu_1284
RCA: Rcas_1231
HAU: Haur_0886
ATM: ANT_24920
PBF: CFX0092_B0571(axeA)
TTR: Tter_0201
PBAS: SMSP2_02919(axeA)
LIE: LIF_A1498
LIS: LIL_12147
LBF: LBF_1671
LST: LSS_18918
BRM: Bmur_1237
BPW: WESB_0039
BIP: Bint_2614
BXY: BXY_25990
FLN: FLA_1626
MUC: MuYL_4073
TMW: THMA_0073
TMQ: THMB_0073
TMX: THMC_0073
TPT: Tpet_0847
TRQ: TRQ2_0870
TNA: CTN_0615
TNP: Tnap_0707
TLE: Tlet_0356
DTU: Dtur_0265
 » show all
Taxonomy
Reference
1  [PMID:4519146]
  Authors
Fujisawa Y, Shirafugi H, Kida M, Nara K, Yoneda M, Kanzaki T.
  Title
New findings on cephalosporin C biosynthesis.
  Journal
Nat New Biol 246:154-5 (1973)
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.41
IUBMB Enzyme Nomenclature: 3.1.1.41
ExPASy - ENZYME nomenclature database: 3.1.1.41
BRENDA, the Enzyme Database: 3.1.1.41
CAS: 52227-71-1

KEGG   REACTION: R03062Help
Entry
R03062                      Reaction                               

Name
Cephalosporin-C acetylhdrolase
Definition
Cephalosporin C + H2O <=> Deacetylcephalosporin C + Acetate
Equation
Comment
Ref.1 : Appl. Environ. Microbiol. June 1995, Vol61, No6, p2224-2229
Reaction class
RC00020  C00033_C00916
RC00041  C00916_C03112
Enzyme
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K01060  cephalosporin-C deacetylase [EC:3.1.1.41]
Other DBs
RHEA: 22599

KEGG   REACTION: R03064Help
Entry
R03064                      Reaction                               

Name
acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Definition
Cephalosporin C + CoA <=> Deacetylcephalosporin C + Acetyl-CoA
Equation
Reaction class
RC00004  C00010_C00024
RC00041  C00916_C03112
Enzyme
Pathway
rn00311  Penicillin and cephalosporin biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K12747  deacetylcephalosporin-C acetyltransferase [EC:2.3.1.175]
Other DBs
RHEA: 23863

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