KEGG   ORTHOLOGY: K13032
Entry
K13032                      KO                                     

Name
PH
Definition
prunasin beta-glucosidase [EC:3.2.1.118]
Pathway
ko00460  Cyanoamino acid metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K13032  PH; prunasin beta-glucosidase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.2  Glycosylases
   3.2.1  Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
    3.2.1.118  prunasin beta-glucosidase
     K13032  PH; prunasin beta-glucosidase
Other DBs
RN: R02558
GO: 0050224
CAZy: GH1
Genes
PPER: 18773049 18773316 18773594 18773978 18775073 18783761
PMUM: 103326467 103341808 103343774
PAVI: 110750143 110764648
MDM: 103447738
PXB: 103930715 103944961 103954605
Reference
  Authors
Zhou J, Hartmann S, Shepherd BK, Poulton JE
  Title
Investigation of the microheterogeneity and aglycone specificity-conferring residues of black cherry prunasin hydrolases.
  Journal
Plant Physiol 129:1252-64 (2002)
DOI:10.1104/pp.010863

KEGG   ENZYME: 3.2.1.118
Entry
EC 3.2.1.118                Enzyme                                 

Name
prunasin beta-glucosidase;
prunasin hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
prunasin beta-D-glucohydrolase
Reaction(IUBMB)
(R)-prunasin + H2O = D-glucose + mandelonitrile [RN:R02558]
Reaction(KEGG)
R02558
Substrate
(R)-prunasin [CPD:C00844];
H2O [CPD:C00001]
Product
D-glucose [CPD:C00031];
mandelonitrile [CPD:C00561]
Comment
Highly specific; does not act on amygdalin, linamarin or gentiobiose. (cf. EC 3.2.1.21 beta-glucosidase).
History
EC 3.2.1.118 created 1989
Pathway
ec00460  Cyanoamino acid metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13032  prunasin beta-glucosidase
Genes
PPER: 18773049 18773316 18773594 18773978 18775073 18783761
PMUM: 103326467 103341808 103343774
PAVI: 110750143 110764648
MDM: 103447738
PXB: 103930715 103944961 103954605
Reference
1
  Authors
Kuroki, G., Lizotte, P.A. and Poulton, J.E.
  Title
L-beta-Glycosidases from Prunus serotina EHRH and Davallia trichomanoides.
  Journal
Z Natursforsch C: Biosci 39:232-239 (1984)
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.118
IUBMB Enzyme Nomenclature: 3.2.1.118
ExPASy - ENZYME nomenclature database: 3.2.1.118
BRENDA, the Enzyme Database: 3.2.1.118
CAS: 9023-41-0

KEGG   REACTION: R02558
Entry
R02558                      Reaction                               

Name
(R)-Prunasin beta-D-glucohydrolase
Definition
Prunasin + H2O <=> Mandelonitrile + D-Glucose
Equation
Reaction class
RC00397  C00561_C00844
RC00746  C00031_C00844
Enzyme
3.2.1.21        3.2.1.118
Pathway
rn00460  Cyanoamino acid metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K01188  beta-glucosidase [EC:3.2.1.21]
K05349  beta-glucosidase [EC:3.2.1.21]
K05350  beta-glucosidase [EC:3.2.1.21]
K13032  prunasin beta-glucosidase [EC:3.2.1.118]
Other DBs
RHEA: 16492

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