KEGG   ORTHOLOGY: K13306Help
Entry
K13306                      KO                                     

Name
fcd
Definition
dTDP-4-dehydro-6-deoxyglucose reductase [EC:1.1.1.266]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13306  fcd; dTDP-4-dehydro-6-deoxyglucose reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.266  dTDP-4-dehydro-6-deoxyglucose reductase
     K13306  fcd; dTDP-4-dehydro-6-deoxyglucose reductase
BRITE hierarchy
Other DBs
RN: R05687
GO: 0050573
Genes
AG: AAS99161(fcf1) ABM68324(fcd)
TaxonomyKoalaUniProt
Reference
  Authors
Zayni S, Steiner K, Pfostl A, Hofinger A, Kosma P, Schaffer C, Messner P
  Title
The dTDP-4-dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T.
  Journal
Glycobiology 17:433-43 (2007)
DOI:10.1093/glycob/cwl084
  Sequence

KEGG   ENZYME: 1.1.1.266Help
Entry
EC 1.1.1.266                Enzyme                                 

Name
dTDP-4-dehydro-6-deoxyglucose reductase;
dTDP-4-keto-6-deoxyglucose reductase;
dTDP-D-fucose:NADP+ oxidoreductase;
Fcf1;
dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
dTDP-alpha-D-fucopyranose:NAD(P)+ oxidoreductase
Reaction(IUBMB)
dTDP-alpha-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD(P)H + H+ [RN:R05687]
Reaction(KEGG)
Substrate
dTDP-alpha-D-fucopyranose [CPD:C07277];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
dTDP-4-dehydro-6-deoxy-alpha-D-glucose [CPD:C11907];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzymes from the Gram-negative bacteria Aggregatibacter actinomycetemcomitans and Escherichia coli O52 are involved in activation of fucose for incorporation into capsular polysaccharide O-antigens [1,3]. The enzyme from the Gram-positive bacterium Anoxybacillus tepidamans (Geobacillus tepidamans) is involved in activation of fucose for incorporation into the organism's S-layer [2]. The enzyme from Escherichia coli O52 has a higher catalytic efficiency with NADH than with NADPH [3].
History
EC 1.1.1.266 created 2001, modified 2013
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13306  dTDP-4-dehydro-6-deoxyglucose reductase
Reference
1  [PMID:10358040]
  Authors
Yoshida Y, Nakano Y, Nezu T, Yamashita Y, Koga T.
  Title
A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose.
  Journal
J Biol Chem 274:16933-9 (1999)
DOI:10.1074/jbc.274.24.16933
Reference
2  [PMID:17202151]
  Authors
Zayni S, Steiner K, Pfostl A, Hofinger A, Kosma P, Schaffer C, Messner P
  Title
The dTDP-4-dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T.
  Journal
Glycobiology 17:433-43 (2007)
DOI:10.1093/glycob/cwl084
  Sequence
Reference
3  [PMID:19019146]
  Authors
Wang Q, Ding P, Perepelov AV, Xu Y, Wang Y, Knirel YA, Wang L, Feng L
  Title
Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52.
  Journal
Mol Microbiol 70:1358-67 (2008)
DOI:10.1111/j.1365-2958.2008.06449.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.266
IUBMB Enzyme Nomenclature: 1.1.1.266
ExPASy - ENZYME nomenclature database: 1.1.1.266
BRENDA, the Enzyme Database: 1.1.1.266

KEGG   REACTION: R05687Help
Entry
R05687                      Reaction                               

Name
dTDP-4-keto-6-Deoxyglucose reductase
Definition
dTDP-D-fucose + NADP+ <=> dTDP-4-oxo-6-deoxy-D-glucose + NADPH + H+
Equation
Reaction class
RC00001  C00005_C00006
RC00182  C07277_C11907
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K13306  dTDP-4-dehydro-6-deoxyglucose reductase [EC:1.1.1.266]
Other DBs
RHEA: 36586

DBGET integrated database retrieval system